Materials Advances
·
2021
·Vol. 2
·期 15
·5150-5159
·
IF 0
Xingxia Zhang,Zhifang Ma,Yue Ke,Yu Xia,Xiaodong Xu,Jingchuan Liu,Yumei Gong,Qiang Shi,Jinghua Yin
Biocompatibility, hemostatic performance and wound healing capability are key limitations for the currently available hemostatic agents. To overcome these problems, a hydrogel inspired by a platelet coagulation mediator is developed in this work as a new class of hemostatic adhesive with improved performance and wound healing capability. The hydrogel is prepared using highly biocompatible serotonin and chondroitin sulfate (CS), both of which are natural components of the body. The structural, physical and biological and hemostatic properties of the hydrogel are characterized in detail. It is demonstrated that serotonin acts as a crosslinker to form adhesive hydrogels and as a blood clotting mediator for rapid hemostasis. Chondroitin sulfate regulates cell behaviors and fates to facilitate wound healing. The serotonin-conjugated chondroitin sulfate hydrogel exhibits improved hemostatic capability in vivo and rapid wound healing after hemostasis. In addition, the wound healing capability of the hydrogel is further improved with the aloe vera powder, confirming the versatility of the hydrogel system. Therefore, chondroitin sulfate–serotonin hydrogels exhibit the potential for effective hemostasis and wound healing.
研究领域:
生物材料
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医学工程
·
再生医学
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产品关联
DOI
ACS Omega
·
2021
·
IF 3.512
Li Zhao,Guiming Niu,Fucheng Gao,Kaida Lu,Zhiwei Sun,Hui Li,Martina Stenzel,Chao Liu,Yanyan Jiang
The development of novel electrode materials for rapid and sensitive detection of neurotransmitters in the human body is of great significance for early disease diagnosis and personalized therapy. Herein, gold nanorod@zeolitic imidazolate framework-8 (AuNR@ZIF-8) core–shell nanostructures were prepared by controlled encapsulation of gold nanorods within a ZIF-8 assembly. The designed AuNR@ZIF-8 nanostructures have uniform morphology, good dispersion, a large specific surface area, and an average size of roughly 175 nm. Compared with individual ZIF-8 and AuNR-modified electrodes, the obtained core–shell-structured AuNR@ZIF-8 nanocomposite structure-modified electrode shows excellent electrocatalytic performance in the determination of dopamine (DA) and serotonin (ST). The designed AuNR@ZIF-8 exhibited a wide linear range of 0.1–50 μM and low detection limit (LOD, 0.03 μM, S/N = 3) for the determination of DA, as well as a linear range of 0.1–25 μM and low LOD (0.007 μM, S/N = 3) for monitoring ST. The improved performance is attributed to the synergistic effect of the high conductivity of AuNRs and multiple catalytic sites of ZIF-8. The good electroanalytical ability of AuNR@ZIF-8 for detection of DA and ST can provide a guide to efficiently and rapidly monitor other neurotransmitters and construct novel electrochemical sensors.
研究领域:
材料科学
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生物医学工程
·
分析化学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION
·
2017
·
IF 1.444
Yi Wu,Wenhui Liu,Qi Li,Yafei Li,Yali Yan,Fang Huang,Xin Wu,Quancheng Zhou,Xugang Shu,Zheng Ruan
Chlorogenic acid (CGA) has many biological properties, including antibacterial, antioxidant and anti‐inflammatory properties, and is one of the most abundant phenolic acids available in the human diet. The aim of this study was to investigate the effects of CGA on regulation of the gut microbiota, and on the levels of free amino acids and 5-hydroxytryptamine (5-HT, serotonin). Ninety-six healthy growing pigs were randomly assigned to two treatment groups: the Ctrl group (control group, standard feed) and the CGA group [standard feed plus 0.05% 3-caffeoylquinic acid (3-CQA)] for 60 days. The diversity of the gut microbiota was increased after CGA supplementation. Changes in these microbes were significantly associated with the serum free amino acid levels and colonic 5-HT level. Compared with the Ctrl group, the levels of serum aspartic acid, threonine, alanine, arginine, and colonic 5-HT were significantly increased (p < .05). These data suggest important roles for CGA in regulating the gut microbiota and increasing the serum free amino acid levels.
研究领域:
营养学
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微生物学
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动物科学
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产品关联
DOI
PubMed
高影响因子
Micromachines
·
2022
·Vol. 13
·期 3
·488
·
IF 3.523
Yun Wang,Mixia Wang,Yuchuan Dai,Yilin Song,Yiding Wang,Botao Lu,Yinghui Li,Xinxia Cai
Research on the intracerebral mechanism of insomnia induced by serotonin (5-HT) deficiency is indispensable. In order to explore the effect of 5-HT deficiency-induced insomnia on brain regions related to memory in rats, we designed and fabricated a microelectrode array that simultaneously detects the electrical activity of the dorsal raphe nucleus (DRN) and hippocampus in normal, insomnia and recovery rats in vivo. In the DRN and hippocampus of insomnia rats, our results showed that the spike amplitudes decreased by 40.16 and 57.92%, the spike repolarization slope decreased by 44.64 and 48.59%, and the spiking rate increased by 66.81 and 63.40%. On a mesoscopic scale, the increased firing rates of individual neurons led to an increased δ wave power. In the DRN and hippocampus of insomnia rats, the δ wave power increased by 57.57 and 67.75%. Furthermore, two segments’ δ wave slopes were also increased in two brain regions of the insomnia rats. Our findings suggest that 5-HT deficiency causes the hyperactivity of neurons in the hippocampus and DRN; the DRN’s firing rate and the hippocampal neuronal amplitude reflect insomnia in rats more effectively. Further studies on alleviating neurons affected by 5-HT deficiency and on achieving a highly effective treatment for insomnia by the microelectrode array are needed.Keywords:microelectrode array;insomnia;electrophysiology;dorsal raphe nucleus;serotonin
研究领域:
神经科学
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电生理学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
METABOLIC ENGINEERING
·
2024
·
IF 6.8
Qingchen Li,Chenxi Li,Jie Zhong,Yukun Wang,Qinghua Yang,Bingmei Wang,Wenjin He,Jianzhong Huang,Shengyuan Lin,Feng Qi
N-methylserotonin (NMS) is a valuable indole alkaloid with therapeutic potential for psychiatric and neurological disorders, and it is used in health foods, cosmetics, and weight loss supplements. However, environmental challenges and low reaction efficiencies significantly hinder cost-effective, large-scale production of NMS in plants or through chemical synthesis. Herein, we have successfully engineered Escherichia coli strains to enhance NMS production from L-tryptophan using whole-cell catalysis. We developed multiple biosynthesis pathways incorporating modules for serotonin (5-hydroxytryptamine, 5-HT), tetrahydromonapterin (MH₄), and S-adenosylmethionine (SAM) synthesis. To enhance MH₄ availability, we employed a high-activity Bacillus subtilis FolE and minimized carbon flux loss through targeted gene knockouts in competitive metabolic pathways, improving 5-HT production. Additionally, we constructed a comprehensive SAM biosynthesis module to facilitate transmethylation by a selected N-methyltransferase fused with ProS2. These engineered modules were coexpressed in two plasmids within the optimized strain NMS-19, producing 128.6 mg/L of NMS in a 5-L bioreactor using fed-batch cultivation—a 92-fold increase over the original strain. This study introduces a viable strategy for NMS production and provides insights into the biosynthesis of SAM-dependent methylated tryptamine derivatives.
研究领域:
生物技术
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代谢工程
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微生物学
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药理学
✓ 已验证
DOI
PubMed
高影响因子
GERONTOLOGY
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2024
·
IF 3.1
Liang Yu,Zhou Yiming,Zhou Can,Cai Xinqi,Liu Li,Wei Fang,Li Guolin
Introduction: While several antidepressants have been identified as potential geroprotectors, the effect and mechanism of sertraline on healthspan remain to be elucidated. Here, we explored the role of sertraline in the lifespan and healthspan of Caenorhabditis elegans. Methods: The optimal effect concentration of sertraline was first screened in wild-type N2 worms under heat stress conditions. Then, we examined the effects of sertraline on lifespan, reproduction, lipofuscin accumulation, mobility, and stress resistance. Finally, the expression of serotonin signaling and aging-related genes was investigated to explore the underlying mechanism, and the lifespan assays were performed in ser-7 RNAi strain, daf-2, daf-16, and aak-2 mutants. Results: Sertraline extended the lifespan in C. elegans with concomitant extension of healthspan as indicated by increasing mobility and reducing fertility and lipofuscin accumulation, as well as enhanced resistance to different abiotic stresses. Mechanistically, ser-7 orchestrated sertraline-induced longevity via the regulation of insulin and AMPK pathways, and sertraline-induced lifespan extension in nematodes was abolished in ser-7 RNAi strain, daf-2, daf-16, and aak-2 mutants. Conclusion: Sertraline promotes health and longevity in C. elegans through ser-7-insulin/AMPK pathways
研究领域:
生物化学
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分子生物学
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衰老研究
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DOI
PubMed
高影响因子
Scientific Reports
·
2016
·Vol. 6
·期 1
·1-8
·
IF 5.228
Li Yu-Tao,Tang Li-Na,Ning Yong,Shu Qing,Liang Feng-Xia,Wang Hua,Zhang Guo-Jun
Acupuncture treatment is amazing but controversial. Up to now, the mechanism of treating diseases by acupuncture and moxibustion is still unclear, especially the occurrence of the molecular events in local acupoints. Herein, we report an extremely stable microsensor by modifying carbon nanotube (CNT) to the tip surface of acupuncture needle and applying this CNT-modified acupuncture needle for real time monitoring of serotonin (5-HT) in vivo . To stabilize CNT modification on the needle tip surface, poly(3,4-ethylenedioxythiophene)(PEDOT) was employed as glue water to stick CNT on the needle. The detection limit of the CNT-modified needle was found to be approximately 50 nM and 78 nM in the PBS and the cell medium, respectively. In addition, the needle showed good selectivity to some inflammatory mediators and some electroactive molecules. For the first time, the CNT-modified needle could be directly probed into rat body for real time monitoring of 5-HT in vivo , showing a great potential for better understanding the mechanism of acupuncture treatment.
研究领域:
生物医学工程
·
神经科学
✓ 已验证
DOI
PubMed
高影响因子
ENVIRONMENTAL TOXICOLOGY
·
2018
·Vol. 34
·期 1
·22-29
·
IF 2.491
Yi Zhang,Cuiyuan Jin,Xiaoyu Wang,Manlu Shen,Jiajie Zhou,Sisheng Wu,Zhengwei Fu,Yuanxiang Jin
Propamocarb (PM) is a type of fungicide, which is widely used in the greenhouse-based production of vegetables and fruits globally. It has been considered to have generally low toxicity. However, the teratogenicity or neurotoxicity for mammals remains unclear. In this study, we aimed to explore its effect on the secretion of neurotransmitters and behavioral impairments. Male adult mice were exposed to 10 and 40 mg/L PM for 2 weeks (acute exposure) or 3 and 10 mg/L PM for 10 weeks (chronic exposure). It was observed that acute or chronic exposure to PM changed the levels of serotonin (5-HT) and dopamine in the serum and colon and the transcription of TPH2 and DRD2 in the colons of mice. In addition, the locomotor test, the open field test, and the Morris water maze analysis also showed that acute exposure to PM caused behavioral impairments to some extent. The results obtained in the present study indicated that PM has the potential to induce neurotoxicity in animals.
研究领域:
毒理学
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神经科学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Danfeng Sun,Hongji Li,Mingji Li,Cuiping Li,Hongli Dai,Dazhi Sun,Baohe Yang
A novel NiO/carbon nanotube (CNT)/poly(3,4-ethylenedioxythiophene) (PEDOT) composite with a coaxial tubular nanostructure was successfully deposited on a glassy carbon electrode (GCE) through electrochemical processes. The outer PEDOT matrix has a high affinity for biomolecules, while the CNT inner layer has a strong electron-transport capacity. Hence, the NiO/CNT/PEDOT/GCE was used for the simultaneous determination of dopamine (DA), serotonin (5-HT), and tryptophan (Trp). Electrochemical responses of the NiO/CNT/PEDOT/GCE sensor were investigated by cyclic voltammetry, electrochemical impedance spectroscopy, and differential pulse voltammetry. Three well-separated oxidation peaks were observed in a mixed system containing DA, 5-HT, and Trp; the 5-HT–DA and Trp–5-HT potential separations were 160 and 324 mV, respectively, owing to the synergistic effect of NiO, CNT, and PEDOT. Linear responses were obtained for DA, 5-HT, and Trp in the 0.03–20, 0.3–35, and 1–41 μM concentration ranges, with detection limits of 0.026, 0.063, and 0.210 μM, respectively. In addition, it was possible to simultaneously determine the three analytes using the NiO/CNT/PEDOT composite, which suggests that the sensor has promising applicability.
研究领域:
分析化学
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生物医学工程
·
材料科学
✓ 已验证
DOI
高影响因子
Carbon Letters
·
2023
·Vol. 33
·期 7
·2129-2139
·
IF 4.5
Tang Shanshan,Liang Axin,Liu Miao,Wang Wei,Zhang Fulai,Luo Aiqin
A glassy carbon electrode modified with a composite consisting of electrodeposited chitosan and carboxylated multi-walled carbon nanotubes (e-CS/MWCNTs/GCE) was used as a working electrode for simultaneous determination of dopamine (DA), serotonin (5-HT) and melatonin (MT), which were related to circadian rhythms. The electrochemical characterizations of the working electrode were carried out via electrochemical impedance spectroscopy and chronocoulometry. It was found that electrochemical modification method, that was cyclic voltammetry, may can cause continuous CS polymerization on MWCNTs surface to form a dense membrane with more active sites on the electrode, and the electrochemically active surface area of e-CS/MWCNTs/GCE obtained was about 7 times that of GCE. The electrochemical behaviour of DA, 5-HT and MT on working electrode were carried out via differential pulse voltammetry and cyclic voltammetry. The results showed that e-CS/MWCNTs/GCE solved the problem that the bare electrode could not detect three substances simultaneously, and can catalyze oxidation potential difference as low as 0.17 V of two substances reaction at the same time, indicating very good electrocatalytic activity. By optimizing the detection conditions, the sensor showed a good linear response to DA, 5-HT and MT in the range of 20-1000 μmol/L, 9-1000 μmol/L and 20-1000 μmol/L, and the detection limits were 12 μmol/L, 10 μmol/L and 22 μmol/L (S/N = 3), respectively. In addition, the proposed sensor was successfully applied to the simultaneous detection of DA, 5-HT and MT in human saliva samples.
研究领域:
分析化学
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生物医学工程
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
PLoS One
·
2014
·Vol. 9
·期 3
·e91775
·
IF 3.534
Renyu Zhang,Xiang Li,Yanan Shi,Yufeng Shao,Kaoxiang Sun,Aiping Wang,Fengying Sun,Wanhui Liu,Di Wang,Jingji Jin,Youxin Li
Triple reuptake inhibitors (TRIs) are currently being developed as a new class of promising antidepressants that block serotonin (5-HT), dopamine (DA) and norepinephrine (NE) transporters, thereby increasing extracellular monoamine concentrations. The purpose of this study was to investigate the effects of LPM570065, a novel TRI and a desvenlafaxine prodrug, on extracellular 5-HT, DA and NE levels in the rat striatum after acute and chronic administration relative to desvenlafaxine, using High Performance Liquid Chromatography (HPLC) and microdialysis. Acute administration was performed by providing rodents with oral solutions (0.06 mmol·kg −1 p.o.), oral suspensions (0.06 mmol·kg −1 p.o.) and intravenous solutions (0.04 mmol·kg −1 i.v.) of LPM570065 and desvenlafaxine. Oral suspensions (0.06 mmol·kg −1 ·day −1 ) of the two drugs were also administered for a 14-day chronic period. HPLC analysis revealed that LPM570065 rapidly penetrated the rat striatum, converted into desvenlafaxine and exhibited larger total exposure compared with the administration of desvenlafaxine. Microdialysis revealed that acute and chronic administration of oral suspension of LPM570065 increased the 5-HT, DA and NE levels more than the relative administration of desvenlafaxine. Unlike desvenlafaxine, acute administration of an intravenous LPM570065 solution did not induce the undesirable 90% decrease in extracellular 5-HT levels. In contrast to the fully dose-dependent elevation of 5-HT induced by desvenlafaxine, the acute administration of LPM570065 showed a capped increase in extracellular 5-HT levels when combined with WAY-100635. Additionally, forced swim test demonstrated that acute and chronic administration of LPM570065 reduced the immobility time more than the relative administration of desvenlafaxine. These data suggest that LPM570065 may have greater efficacy and/or a more rapid onset of antidepressant action than desvenlafaxine and also counterbalance the harmful effects of desvenlafaxine on 5-HT neurotransmission related to 5-HT 1A autoreceptors. Thus, this new class of drugs, TRIs has the potential to provide a new therapeutic mechanism for treating depression.
研究领域:
神经科学
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药理学
·
精神病学
✓ 已验证
DOI
PubMed
高影响因子
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN
·
2020
·Vol. 93
·期 11
·1393-1400
·
IF 4.488
Mool-am-kha Pijika,Themsirimongkon Suwaphid,Saipanya Surin,Saianand Gopalan,Tuantranont Adisorn,Karuwan Chanpen,Jakmunee Jaroon
The design and development of facile, rapid, selective, and sensitive electroanalytical methods for the simultaneous determination of serotonin (ST) along with other interfering species are in huge demand. To address this, herein, we report an efficient electrochemical sensing platform for the individual and simultaneous determination of ST and dopamine (DA) in human serum. The sensing system was judicially constructed by hybrid electrocatalytic nanocomposites composed of carbon nanotubes/nickel oxide/carbon black/Nafion modified screen-printed carbon electrode (CNT-NiO-CB/Nafion/SPCE). The structural, morphological, and electrochemical properties of the as-synthesized/fabricated materials and electrodes were thoroughly analyzed through appropriate instrumentations (scanning/transmission electron microscopy, electron microscopy, X-ray photoelectron spectroscopy, electrochemical impedance spectroscopy, etc.). Besides, the experimental parameters of electrochemical measurements, such as varying amounts (CNT-NiO-CB/Nafion), pH, conditioning potential, time, modulation amplitude, and step potential, have been carefully optimized. The as-fabricated newly designed sensor exhibits superior analytical performance such as wide linear range (0.06–7.50 µM ST and 0.08–6.00 µM DA), low limit detection of (11 nM ST and 11 nM DA), high reproducibility (RSD = 5.74% ST and 7.74% DA) indicating excellent selectivity, stability, sensitivity along with good recovery range.
研究领域:
分析化学
·
生物医学工程
·
材料科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）