BIOSENSORS & BIOELECTRONICS
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2013
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IF 5.437
Xiaoyu Mu,Li Qi,Ping Dong,Juan Qiao,Jian Hou,Zongxiu Nie,Huimin Ma
Gold nanoclusters (Au NCs) possess outstanding physical and chemical attributes that make them excellent scaffolds for the construction of novel chemical and biological sensors. In this study, a simple one-pot synthesis method, employing l -proline as the stabilizer, was presented for preparation of fluorescent Au NCs. This strategy allowed the generation of water-soluble Au NCs within a short time of 15 min. The as-prepared Au NCs exhibited a bluish fluorescence emission at 440 nm and a quantum yield of 2.94%. Based on the aggregation-induced fluorescence quenching mechanism, the Au NCs provided favorable biocompatibility, high sensitivity and good selectivity for the measurement of ferric ion (Fe 3+ ). Furthermore, serum samples were analyzed for the serum iron contents by using this proposed biocompatible fluorescent sensor, indicating the potential value of this Au NCs-based fluorescent sensor for application in biological and clinical analysis.
研究领域:
分析化学
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生物医学
✓ 已验证
DOI
高影响因子
JOURNAL OF LUMINESCENCE
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2015
·
IF 2.719
ShaoZhen Wang,Jianguo Zhang,Hongqi Chen,Lun Wang
A new serum ferritin (SF) biosensor based on fluorescence resonance energy transfer (FRET) from Mn 2+ -doped NaYF 4 :Yb,Tm up-converting rare-earth nanophosphors (UCNPs, donor) to gold nanorods (GNRs, acceptor) was developed in the near-infrared (NIR) region. The as-prepared Mn 2+ -doped NaYF 4 :Yb,Tm UCNPs exhibited a strong emission at 807 nm in the NIR region and were modified with poly-(acrylic acid) (PAA) before conjugating with anti-SF (Ab 1 ). The negatively charged donor (Ab 1 -UCNPs) and the positively charged acceptor (GNRs) were mixed in close proximity due to electrostatic interactions, which quenched the fluorescence emission of the Mn 2+ -doped NaYF 4 :Yb,Tm UCNPs. However, added SF combines with the anti-SF because the binding affinity between SF and Ab 1 is stronger than the electrostatic interactions. Thus, the acceptor separated from the donor, and the fluorescence was restored. Under optimal conditions, the restored fluorescence exhibited a linear relationship with SF concentrations ranging from 0.23 to 16.88 ng/mL and a limit of detection as low as 0.12 ng/mL. The proposed method was also used to monitor SF in human serum samples.
研究领域:
生物化学
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纳米医学
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
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IF 6.2
Jianglan Yuan,Congxin Lei,Yuchen Duan,Hanxin Zeng,Junzhe Ma,Dongliang Chen,Xu Kang
Heme is widely recognized as an effective iron supplement. In vitro experiments suggested a relatively low bioaccessibility of free heme due to its strong hydrophobicity. The study prepared a β-lactoglobulin-heme nanocomplex (β-Lg-He) to improve the bioaccessibility of heme. The results indicated that heme could spontaneously bind to β-Lg mainly through hydrogen bonds and van der Waals forces to form water-soluble and uniform nanocomplexes. Simulated gastrointestinal digestion experiments showed that bioaccessibility of heme in β-Lg-He was more than 80%, while that of free heme was only about 8.0%, and the serum iron levels of mice in β-Lg-He groups were higher than those in heme groups and control groups. The results of in vivo experiments revealed that β-Lg-He could have a more beneficial effect on the intestinal microbiota of mice than free heme, which was an additional function for the high-efficiency iron supplement.
研究领域:
食品科学
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营养生物化学
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分子生物学
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纳米技术
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yuying He,Biyang Zhu,Huamai Qiu,Lijun You,Lianzhu Lin,Sandra Pedisić
Iron dextran complex is commonly used to treat iron deficiency anemia (IDA). In this work, dextran was degraded and oxidized by UV/H 2 O 2 treatment, and the product Dex-90 was chelated with FeCl 3 to prepare the iron dextran complex (Dex-90-Fe). Results showed that UV/H 2 O 2 treatment could significantly decrease the molecular weight of dextran and increase its carboxyl content, making it easier to chelate with iron (III). Moreover, Dex-90-Fe had a high iron content (30.77 % ± 0.87 %) and good thermal stability, which might be formed by Dex-90 with iron (III) via Fe O bond. Furthermore, the therapeutic effects of Dex-90-Fe on IDA were investigated in rats. After oral administration, Dex-90-Fe significantly improved hematological parameters, increased serum iron and serum ferritin, facilitated iron accumulation in the liver and spleen, and regulated the gene expressions of iron homeostasis. In summary, the iron dextran complex Dex-90-Fe prepared from UV/H 2 O 2 -degraded dextran could be developed as an iron supplement.
研究领域:
药剂学
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生物无机化学
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营养学
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血液学
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分子药理学
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药物代谢
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纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2024
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IF 4.9
Jun-Xi Zhou,Ting Wu,Qing-Yin Ni,Bo-Yi Yuan,Shu-Chang Zhao,Yun-Yun Sun,Jing Huang,Min-Lin Zou,Rui-Lin Liu
No reports disclosed that whether the plastic centrifuge tube with intrinsic photoenzyme activity can be used for the specific sensing of pharmaceuticals and biomarkers in biofluids so far. We herein report the plastic eppendorf (EP) tube as both reaction vessel and sunlight-activated plastic mimic enzyme for colorimetric/smartphone dual-mode detection of D-penicillamine (D-PA) and iron (Ⅲ) ions in goat and rabbit serums accordingly. The photooxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) combined with simulated sunlight-activated EP tube was selectively inhibited by adding D-PA, and the absorbance at 652 nm of oxidized TMB decreased. Good linearity between the absorbance variation (or R/(R + G + B)) and D-PA level ranging from 10 to 300 µM was obtained, and the limits of detection (LOD) were determined as 8.50 μM (colorimetric) and 6.92 μM (smartphone). Furthermore, the polyvinyl alcohol hydrogel encapsulated TMB in EP tube as a smartphone biosensor had been triumphantly used to monitor D-PA level in goat serum. Interestingly, the adsorption band of photo-oxidized EP tube-TMB system inhibited by D-PA gradually increased as the enhancement of iron (Ⅲ) ions concentration. Based on the “on–off-on” effect, a sensitive and selective method was further used for selectively sensing iron (Ⅲ) in rabbit serum. Notably, the dual-mode sensing platforms exhibited outstanding detection performance, with LOD values as low as 0.50 µM and 0.65 µM respectively. In short, this work not only excavated a photoactivated-plastic mimics, but also developed a robust, low-cost, simple and sensitive biosensor for simultaneous detection of D-PA and Fe (Ⅲ) ions in biofluids. The current photoactivated sensor does not requires the introduction of exogenous catalyst except for simulated sunlight, plastic tube and chromogenic substrate.
研究领域:
分析化学
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生物化学
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材料科学
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生物技术
✓ 已验证
产品关联
DOI
高影响因子
Pharmaceuticals
·
2022
·Vol. 15
·期 10
·1177
·
IF 5.215
Yini Dang,Qing He,Siyu Yang,Huaiqing Sun,Yin Liu,Wanting Li,Yi Tang,Yu Zheng,Ting Wu
Objectives: Despite significant advances in neuroscience, the mechanisms of AD are not fully understood. Single-cell RNA sequencing (scRNA-seq) techniques provide potential solutions to analyze cellular composition of complex brain tissue and explore cellular and molecular biological mechanisms of AD. Methods: We investigated cellular heterogeneity in AD via utilization of bioinformatic analysis of scRNA-seq in AD patients and healthy controls from the Gene Expression Omnibus (GEO) database. The “GOplot” package was applied to explore possible biological processes in oligodendrocytes, astrocytes, and oligodendrocyte progenitor cells (OPCs). Expression patterns and biological functions of differentially expressed genes (DEGs) from scRNA-seq data were validated in RNA sequencing data. DEGs in astrocytes interacted with ferroptosis-related genes in FerrDb. CCK-8 and EdU assays were performed to measure cell proliferation ability. ROS, Fe2+level, mitochondrial membrane potentials, iron concentrations, and total iron binding capacity (TIBC) in serum were evaluated. Y-maze and elevated maze were used to measure anxiety-like behavior. Autonomous and exploration behaviors or learning and memory ability in mice were analyzed using open field test and novel object recognition test. Results: Multiple clusters were identified, including oligodendrocytes, astrocytes, OPCs, neurons, microglia, doublets, and endothelial cells. Astrocytes were significantly decreased in AD, while oligodendrocytes and OPCs increased. Cell-to-cell ligand–receptor interaction analysis revealed that astrocytes, neurons, and OPCs mainly established contacts with other cells via the NRG3–ERBB4 ligand–receptor pair. GO and KEGG analyses found that astrocytes were enriched in the ferroptosis pathway. FTH1 and SAT1 in astrocytes were identified as hub mRNAs associated with ferroptosis. Serum iron concentration of 5xFAD mice was higher than that of WT, and emotional and cognitive function were significantly impaired as compared to WT. Serum iron concentration was negatively correlated with number of astrocytes and percentage of time spent entering the novelty arm in the Y-maze test, while it was positively correlated with percentage of time spent in the central area. Meanwhile, number of astrocytes was negatively correlated with percentage of time spent in the central area, while it was positively correlated with percentage of time spent entering the novelty arm. Conclusions: Through scRNA-seq analysis, we found that ferroptosis was activated in astrocytes and may contribute to the pathophysiological process in the entorhinal cortex. FTH1 and SAT1 were identified to impact astrocyte ferroptosis. Emotional and cognitive impairment in AD was associated with astrocyte ferroptosis. Our findings provide clues to reveal the pathophysiological processes following AD at the cellular level and highlight potential drug targets for the treatment of AD.Keywords:Alzheimer’s disease;single-cell RNA sequencing;astrocytes;ferroptosis
研究领域:
神经科学
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分子生物学
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生物信息学
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神经退行性疾病
✓ 已验证
DOI
PubMed
高影响因子
Nutrition & Metabolism
·
2022
·Vol. 19
·期 1
·1-9
·
IF 4.654
Qiu Fubin,Li Rui,Gu Siyu,Zhao Yimin,Yang Linxue
Background Iron and vitamin D (VD) is essential to health. Previous studies have shown that iron homeostasis has a potential effect on VD metabolism, but the mechanism is not fully understood.ObjectivesTo explore the relationship between VD metabolism and iron metabolism, as well as the regulatory mechanism of iron on VD metabolism.Methods 40 male rats were fed adaptively for 7 days and randomly divided into control (C, n = 6 normal diet) group and model (M, n = 24 iron deficient diet) by simple randomization, the latter was used to establish iron deficiency anemia (IDA) model. After 6 weeks of feeding, the M group was randomly divided into: iron deficiency group (DFe), low iron group (LFe), medium iron group (MFe) and high iron group (HFe) by block randomization. Different doses of iron dextran (based on iron content (100 g·bw·d)): 0, 1.1, 3.3 and 9.9 mg) were given respectively. After 4 weeks, the rats were anesthetized with 8% chloral hydrate, Blood (collected from the abdominal aorta), liver and kidney tissues were collected. The serum and tissues were separately packed and frozen at -80℃ for testing.Results The results showed that the levels of hemoglobin (Hb), red blood cell (RBC), serum iron (SI), liver iron, and kidney iron in DFe group were lower than those in the other four groups, while the levels of total iron-binding capacity (TIBC), transferrin (TF) and transferrin receptor (Tfr) in DFe group were higher than those in other groups; The serum levels of 25-(OH)D3 and 1,25-(OH)2D3 in DFe group were significantly lower than those in C group (P < 0.05). The correlation analysis showed that the levels of 25-(OH)D3 and 1,25-(OH)2D3 were negatively correlated with TIBC, TF and Tfr no correlation with SI. Western blotting, immunofluorescence, and q-PCR results showed that compared with C group, the protein and gene expressions of CYP2R1, CYP27A1, and CYP24A1 in DFe group were down-regulated, and the expression of CYP27B1 protein and gene was up-regulated in DFe group.Conclusion Iron may be involved in the metabolism of VD3 by regulating the expression of VD3 hydroxylase, suggesting that appropriate iron supplementation might promote the activation of VD3.
研究领域:
营养学
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生物化学
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药理学
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动物生理学
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DOI
PubMed
高影响因子
Nutrients
·
2023
·Vol. 15
·期 6
·1522
·
IF 5.9
Jiyong Yin,Jiangping Niu,Junsheng Huo,Jing Sun,Jian Huang,Chaoqun Sun
We attempted to construct and evaluate a novel detection method to realize simultaneous detection based on a multiplex liquid protein chip technique for nine nutrition-and-health-related protein markers to meet the requirement of an accurate, simultaneous and comprehensive analysis of the proteomics of nutrition and health. The lower limits of detection, biological limits of detection and regression equations of serum ferritin (SF), soluble transferrin receptor (sTfR), c-reactive protein (CRP), retinol-binding protein4 (RBP4), apolipoprotein B (ApoB), alpha-fetoprotein (AFP), prealbumin (PA), carcino-embryonic antigen (CEA) and D-Dimmer (D-D) were determined after a series of optimal experiments. Then, the results of the methodological evaluation for this novel method indicated that the accuracies were between 70.12% and 127.07%, the within-run precisions were between 0.85% and 7.31%, the between-run precisions were between 3.53% and 19.07%, the correlation coefficients between this method and other methods were above 0.504 (p< 0.05), and the direct bilirubin (DBIL) of low concentration and the indirect bilirubin (IBIL) of high concentration could not interfere with the detected results of nine indicators. The novel multiplex detection method, which can increase accuracy and improve the ability of comprehensive analysis, can basically meet the requirement of detection and the diagnosis of the proteomics of nutrition and health.Keywords:liquid protein chip;nutrition and health;protein marker;multiplex detection
研究领域:
蛋白质组学
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临床生化
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营养科学
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医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Jing Jing,Zhijun Zhang,Zhiyong Hu,Xuemei Ma,Jianlan Cui,Hailin Zhu,Xueli Bai,Lijun Zhai
Iron deficiency anemia (IDA) is the most common nutritional disease worldwide. In this study, a low methoxyl pectin (LMP)‑iron(III) complex was prepared. The physicochemical and structural properties were characterized by HPSEC, HPIC, CV, FTIR, 1 H NMR, XRD, SEM and CD. The results showed that iron increased the molecular weight of the LMP‑iron(III) from 11.50 ± 0.32 to 12.70 ± 0.45 kDa and improved its crystallinity. Moreover, the findings demonstrated that -OH and -COOH groups in LMP coordinate with Fe 3+ to form β -FeOOH. The water-holding capacity, emulsion stability, and antioxidant activities of the LMP‑iron(III) were lower than those of LMP. Furthermore, the therapeutic effects of LMP‑iron(III) on IDA were investigated in rats. Following LMP‑iron(III) supplementation, compared with the model group, the administration of LMP‑iron(III) significantly increased the body weight, hemoglobin concentration, and serum iron concentration as well as decreased free erythrocyte protoporphyrin concentration. Therefore, the LMP‑iron(III) can potentially treat IDA in rats experiments, providing a theoretical basis for the development of a promising iron supplement.
研究领域:
营养学
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生物化学
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药学
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动物科学
✓ 已验证
DOI
PubMed
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2021
·
IF 7.328
Lu Jia,Zukun Yang,Liuxu Sun,Qian Zhang,Yu Guo,Yulian Chen,Yang Dai,Yang Xia
Scaffold implantation for the repair of oral bone defects involves an interplay between the scaffold biomaterial and the microenvironment. However, previous studies on this subject have only considered the effects of the immune system and largely ignored those of the oral microbiota. Accordingly, in the present study, we prepared composite scaffolds comprising a three-dimensional poly( l -lactide- co -glycolide) matrix with a superparamagnetic iron oxide nanoparticle (SPION) coating and used a rat model to evaluate their palate-bone-regenerating effects and their interaction with the oral microbiota. It was found that the SPION coated scaffold induced better bone regeneration than that achieved by the controls. Furthermore, it significantly decreased the operational taxonomic units (OTU) numbers as determined by 16 s rRNA gene sequencing, and also resulted in decreased Chao and ACE alpha diversity indexes compared with those of the controls. However, it had no effect on beta diversity. SPION coated scaffolds caused a shift in oral bacterial composition characterized by a decrease in the Clostridium spp. population, and the dominant flora being Proteobacteria . Furthermore, SPION coated scaffolds upregulated the concentration of serum iron, hepcidin, and P1NP. Thus, SPION coated scaffolds enhanced bone regeneration, and this effect was partly related to alteration of the oral microbiota by the antibacterial effects of SPION. Our findings provide a better understanding of the role of oral microbiota in oral bone regeneration and how SPION coated scaffolds can be used to enhance it.
研究领域:
生物材料
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组织工程
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口腔生物学
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微生物学
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再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Immunology
·
2022
·
IF 8.786
Lifang Ye,Yu Zuo,Fang Chen,Yuetong Xu,Puli Zhang,Hongxia Yang,Qinglin Peng,Guochun Wang,Xiaoming Shu
Objective To evaluate adrenomedullin mRNA levels in the peripheral blood mononuclear cells (PBMCs) of patients with dermatomyositis (DM) as well as their correlation with the severity of interstitial lung disease (ILD). Methods A total of 41 DM patients and seven immune-mediated necrotizing myopathy (IMNM) patients were recruited, in addition to 21 healthy controls (HCs). The adrenomedullin mRNA levels in PBMCs were measured via quantitative reverse-transcription real-time polymerase chain reaction (qRT-PCR). The associations between adrenomedullin expression levels and major clinical, laboratory, pulmonary function parameters and the prognosis of patients with DM-related ILD (DM-ILD) were analyzed. Immunohistochemical analysis was performed on lung tissues of DM-ILD patients to determine adrenomedullin expression. Results Adrenomedullin mRNA levels in PBMCs were significantly higher in DM patients than in IMNM patients and HCs (p = 0.022 and p<0.001, respectively). Among DM patients, the levels were significantly higher in those with rapidly progressive ILD (RP-ILD) than in those with chronic ILD (p = 0.002) or without ILD (p < 0.001). The adrenomedullin mRNA levels in DM-ILD were positively correlated with serum ferritin (r =0.507, p =0.002), lactate dehydrogenase (LDH) (r =0.350, p =0.045), and lung visual analog scale (VAS) (r=0.392, p=0.021) and were negatively correlated with pulmonary function test parameters, including predicted forced vital capacity (FVC)% (r = −0.523, p = 0.025), forced expiratory volume in 1 s (FEV1)% (r = -0.539, p = 0.020), and diffusing capacity of carbon monoxide (DLco)% (r = -0.495, p = 0.036). Immunohistochemical analysis of adrenomedullin confirmed higher expression in the alveolar epithelial cells and macrophages of DM patients with RP-ILD. Among the DM patients with ILD, the six decedents exhibited higher adrenomedullin levels than the 28 survivors (p = 0.042). The cumulative survival rate was significantly lower (62.5% vs . 100%, P = 0.005) in patients with an adrenomedullin level > 0.053 than in those with a level <0.053. Conclusions Adrenomedullin levels are upregulated in DM patients with RP-ILD and are associated with ILD severity and poor prognosis. Adrenomedullin may be a potential prognostic biomarker in DM patients with ILD, although need further investigation.
研究领域:
风湿病学
·
肺科学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）