Materials Today Bio
·
2024
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IF 8.7
Hui Yang,Sirui Huang,Xinwei Zhu,Yasi Chen,Chunming Xu,Ruohan Li,Pan Bu,Yufan Jiang,Changwei Li,Jie Yang,Zhenyi Chen,Weijie Peng,Lin Liu
Critical-sized bone defects present a formidable challenge in tissue engineering, necessitating innovative approaches that integrate osteogenesis and angiogenesis for effective repair. Inspired by the hierarchical porous structure of natural bone, this study introduces a novel method for the scalable production of ultra-long, copper-doped hydroxyapatite (Cu-HAp) fibers, utilizing the rapid gelation properties of guar gum (GG) under controlled conditions. These fibers serve as foundational units to fabricate three-dimensional porous scaffolds with a biomimetic hierarchical architecture. The scaffolds exhibit a broad pore size distribution (1–500 μm) and abundant nanoporous features, mimicking the native bone extracellular matrix. Physicochemical characterization and in vitro assays demonstrated that the copper doping significantly enhanced osteogenic and angiogenic activities, with optimized concentrations (0.8 % and 1.2 % Cu) facilitating the upregulation of osteogenesis-related genes and proteins, as well as promoting endothelial cell proliferation. In vivo studies further confirmed the scaffolds' efficacy, with the 1.2Cu-HAp group showing a remarkable increase in bone regeneration (bone volume/total volume ratio: 35.7 ± 1.87 %) within the defect site. This research offers a promising strategy for the rapid fabrication of multifunctional scaffolds that not only support bone tissue repair but also actively accelerate the healing process through enhanced vascularization.
研究领域:
生物材料学
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组织工程学
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医学
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DOI
PubMed
高影响因子
Biomaterials Science
·
2020
·Vol. 8
·期 8
·2164-2174
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IF 6.183
Yuqing Niu,Guochang Liu,Chuangbi Chen,Ming Fu,Wen Fu,Zhang Zhao,Huimin Xia,Florian J. Stadler
Reconstruction and functional rehabilitation of the long urethra in males is one of the difficult tasks in urological treatment. Although many kinds of tissue-engineered urethra scaffold grafts have been successfully used in animals and even clinical research of urethra reconstruction, they all have the disadvantages of slow vascularization in scaffolds, which may lead to complications such as stricture and blockage of the urethra. Here, an amphiphilic polyurethane tubular nanofiber scaffold with a hierarchical structure was designed as a urethral scaffold. The scaffold can regulate the phenotypic expression of epithelial cells (ECs) and smooth muscle cells (SMCs) in vitro and in vivo. Upon transplantation into the Beagle puppy's defective urethral site, the engineered PU-ran tubular scaffold graft, rich in seeded cell–matrix bio-interfaces, could induce local neo-vascularization in a controlled way, which facilitated lumen epithelialization and functional rehabilitation. This is favorable for urethral tissue-oriented reconstruction. These findings suggest the pivotal role of nano-topographical and biochemical features in the vascularized biomimetic scaffold design for efficacious urethral reconstruction.
研究领域:
组织工程
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泌尿外科
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生物材料
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再生医学
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产品关联
DOI
PubMed
高影响因子
Science China-Chemistry
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2013
·Vol. 56
·期 12
·1701-1709
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IF 1.327
Chen ZhiPing,Wang Wei,Guo Lei,Yu YanYan,Yuan Zhi
For the requirement of preliminary vascularization, the scaffolds for thick tissue engineering should possess not only good cell affinity, but also anticoagulant ability. In this paper, an enzymatically crosslinked hydrogel scaffold based on sulfated chitosan (SCTS) was prepared. Firstly, sulfated chitosan-hydroxyphenylpionic acid (SCTS-HPA) conjugate was synthesized, and its structure was identified by FITR and 1 H NMR. And then an enzymatically crosslinked hydrogel was prepared in the presence of horseradish peroxidase (HRP) and hydrogen peroxide (H 2 O 2 ). The gelation time, mechanical property, morphology and cytotoxicity to human umbilical vein endothelial cells (HUVECs) of the hydrogel was evaluated in vitro , the tissue compatibility of SCTS scaffold was studied in vivo . The results showed that the gelation time, mechanical property, morphology of the dehydrated hydrogel could be controlled by the HRP and H 2 O 2 concentration. The cytotoxicity test showed that the hydrogel extracts had no cytotoxicity to HUVECs. The in vivo assay indicated that SCTS-HPA scaffold showed good tissue compatibility, and no thrombus formation. All these results indicated that the SCTS-HPA scaffold could be used as thick tissue engineering scaffold.
研究领域:
生物材料学
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组织工程学
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再生医学
✓ 已验证
DOI
高影响因子
Stem Cell Research & Therapy
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2020
·Vol. 11
·期 1
·1-11
·
IF 5.116
Yang Zhi,Song Zhicheng,Nie Xin,Guo Kaijin,Gu Yan
Background Biological composite scaffolds are increasingly being used in abdominal wall reconstruction but still have certain shortcomings. The present study describes here a novel three-dimensional (3D) scaffold fabricated by combining 3D printing (3DP) and electrospinning (ESP). Methods Biological composite scaffolds are composed of integrated 3DP interconnected macrofiber and random ESP microfiber networks. The 3DP scaffold retains intact 3D architecture and mechanical properties, while the ESP network serves as a cell entrapment system at the extracellular matrix (ECM) scale. Biological composite scaffolds are implanted in a defective rat abdominal wall to detect if it could induce early vascularization and reconstruction of the tissue defect. Results SEM analysis reveals a pore diameter of 424.47 ± 58.49 μm and a porosity of 70.46 ± 2.48% for biological composite scaffolds. In the in vitro test of cell proliferation, biological composite scaffolds do not affect rat dermal fibroblast proliferation in a time- and dose-dependent manner. The animal experiments show tissue remodeling and early angiogenesis as compared to 3DP scaffolds. Conclusions Our experiment prepares a biological scaffold with both a macro- and microscale structure by ESP and 3DP technology. Thus, the integration of 3DP and ESP techniques provides a new set of smart scaffolds for abdominal wall defect and hernia repair.
研究领域:
生物材料学
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组织工程学
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再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Bioengineering and Biotechnology
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2022
·
IF 6.064
Yiding Shen,Kai Fang,Yun Xiang,Keyuan Xu,Liang Yu,Jiaquan Chen,Pingping Ma,Kaiyong Cai,Xinkun Shen,Jinsong Liu
Titanium (Ti) implants have been widely used for the treatment of tooth loss due to their excellent biocompatibility and mechanical properties. However, modifying the biological properties of these implants to increase osteointegration remains a research challenge. Additionally, the continuous release of various metal ions in the oral microenvironment due to fluid corrosion can also lead to implant failure. Therefore, simultaneously improving the bioactivity and corrosion resistance of Ti-based materials is an urgent need. In recent decades, micro-arc oxidation (MAO) has been proposed as a surface modification technology to form a surface protective oxide layer and improve the comprehensive properties of Ti. The present study doped nano silicon nitride (Si 3 N 4 ) particles into the Ti surface by MAO treatment to improve its corrosion resistance and provide excellent osteoinduction by enhancing alkaline phosphatase activity and osteogenic-related gene expression. In addition, due to the presence of silicon, the Si 3 N 4 -doped materials showed excellent angiogenesis properties, including the promotion of cell migration and tubule formation, which play essential roles in early recovery after implantation.
研究领域:
生物材料
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生物医学工程
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
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2018
·
IF 5.08
Danyu Yao,Zhiyong Qian,Jin Zhou,Ge Peng,Gang Zhou,Haifeng Liu,Yubo Fan
Rapid neovascularization within scaffolds is critical for the regeneration of thick complex tissues. The surface immobilization of peptides and other active molecules have been explored to improve the vascularization capacity of implants. However, the rapid degradation of these molecules, the reaction conditions and cross-linking usually result in decreased vascularization capability. Here, we introduced a temperate, all-aqueous process to achieve bulk porous silk fibroin (SF) scaffolds. A temperature controlled process was used to induce the water stable structure by SF self-assembly. Arg-Glu-Asp-Val (REDV) peptides were added into SF solution and fixed within SF scaffolds during the self-assembly process. The results showed that the functionalized scaffolds markedly promoted the adhesion of endothelial cells in vitro . Moreover, the in vivo studies indicated enhanced cell infiltration in the bulk functionalized SF scaffolds and impressive vascularization at 4 weeks post-implantation. The functionalized scaffolds demonstrated excellent vascularization capability, providing an exciting biomaterial option for thick tissue regeneration.
研究领域:
生物材料
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组织工程
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再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
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2022
·
IF 7.392
Yi Wang,Linhao Hou,Yiqian Huang,Pengfei Wei,Liya Sun,Yan Zhang,Xueqiao Yu,Shiqing Ma,Wei Jing,Bo Zhao,Huachong Ma
Chronic wounds in diabetes take a lifetime risk of developing into diabetic foot ulcers (DFUs). Conventional wound dressings and commercial products fail for ideal tissue regeneration in DFUs due to the incapacity to promote vascularization and prevent bacterial infections during wound repair. In this study, the small intestinal submucosa (SIS) with enriched vascularized bioactive factors, was chemically decorated by 2-methacryloyloxy ethyl phosphorylcholine (MPC) through Michael-type addition to be endowed anti-fouling ability, in order to treat chronic wound regeneration in diabetes. In the diabetic skin injury experiment, we established the animal model by 5-mm diameter defects in the SD rats and implanted the bio-patches to the located sites. The SIS-MPC bio-patch showed superior advantages in bacterial anti-adhesion (100-fold foul resistance than commercial patches), while maintained affinity for fibroblast growth and collagen formation, as demonstrated by immunohistochemical staining from the local tissues, outperforming commercial wound dressings in tissue regeneration, which was attributed to acceleration in neovascularization and inflammation modulation through TGF-β and MAPK pathway. This novel SIS-MPC bio-patch would be a promising candidate for medical translations into diabetes and chronic wound treatments.
研究领域:
生物医学工程
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组织工程
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伤口愈合
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糖尿病研究
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2025
·
IF 4.9
Han Yan,Yuhao Liao,Weixiong Chen,Junnan Luo,Dongdong Li,Jilong Wang,Faxin Liu
Recently, osteoporosis has been a significant clinical challenge for the failed bone regeneration and vascularization. In this study, we developed a multifunctional nanoparticle system to stimulate bone regeneration and vascularization. Poly(D, L-lactide) (PLA)-based nanoparticles were fabricated to encapsulate icariin, which is renowned for its osteogenic potential. Subsequently, these nanoparticles were coated with red blood cell membranes to enhance biocompatibility. To further improve targeting specificity, a bone-targeted polymer-lipid consisting of alendronate-modified PEG-DSPE was synthesized and incorporated into the cell membrane coating. The delivery systems were designed to modulate bone marrow mesenchymal stem cells (BMSCs), thereby enhancing both osteogenesis and angiogenesis. Our findings demonstrate that the therapeutic system could enhance bone formation and vascularization in vitro , indicating its potential as an advanced treatment strategy for osteoporosis. This study provides a promising nanoplatform for the development of multifunctional nanomedicines aimed at improving bone health and vascular integration.
研究领域:
药学
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生物医学工程
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骨科学
✓ 已验证
产品关联
DOI
高影响因子
Science Bulletin
·
2025
·
IF 21.1
Zhikun Huan,Jingbo Li,Xinyue Cao,Minhui Lu,Yunru Yu,Ling Li
Current research on islet transplantation is aimed at prolonging the survival time and enhancing the functionality of the transplantation system. Owing to the good biocompatibility of hydrogels and their ability to provide mechanical support and immunological isolation of islet cells, hydrogel encapsulation of islets offers an innovative solution for islet transplantation. In this work, inspired by the extracellular matrix structure of the pancreatic islets, an injectable self-healing hydrogel loaded with islet-integrated microfiber scaffolds was constructed for islet transplantation. The short microfiber was fabricated by introducing a gaseous phase into microfluidic spinning, causing the continuous microfibers to break into short segments due to bubble rupture. These short microfibers provide mechanical support for pancreatic islets and can be integrated into a self-healing matrix to form a vascularized hydrogel through Schiff base bonding of oxidized sodium alginate aldehyde groups with carboxymethyl chitosan amino groups. With the loading of human umbilical vein endothelial cells and vascular endothelial growth factor, this composite is both injectable and provides mechanical support for the grafts, extends islet survival time, and improves islet function. In vivo experiments further confirm that this bioinspired composite system minimizes implantation-associated trauma while promoting neovascularization at the graft site in diabetic rodents, thereby achieving prolonged glycemic control compared to non-vascularized systems. These findings demonstrate that this hierarchically structured multifunctional graft platform has substantial research value and extensive therapeutic potential in the fields of cell therapy and tissue engineering for the treatment of diabetes and related diseases.
研究领域:
生物材料学
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细胞治疗
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糖尿病研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2019
·
IF 5.122
Wang Shoubao,Xiong Yao,Chen Jingting,Ghanem Abdulsamad,Wang Yinmin,Yang Jun,Sun Binbin
Full-thickness skin wounds are common and could be a heavy physical and economic burden. With the development of three dimensional (3D) printing technology, skin-like constructs have been fabricated for skin wound healing and regeneration. Although the 3D printed skin has great potential and enormous advantages before vascular networks can be well-constructed, living cells are not recommended for 3D skin printing for in vivo applications. Herein, we designed and printed a bilayer membrane (BLM) scaffold consisting of an outer poly (lactic-co-glycolic acid) (PLGA) membrane and a lower alginate hydrogel layer, which respectively mimicked the skin epidermis and dermis. The multi-porous alginate hydrogel of the BLM scaffolds promoted cell adhesion and proliferation in vitro, while the PLGA membrane prevented bacterial invasion and maintained the moisture content of the hydrogel. Skin regeneration using the bilayer scaffold was compared with that of PLGA, alginate hydrogel and the untreated defect in vivo. Tissue samples were analyzed using histopathological and immunohistochemical staining of CD31. In addition, mRNA expression levels of collagen markers [collagen type 1 alpha 1 (COL1a1) and collagen type 3 alpha 1 (COL3a1)] and inflammatory markers [interleukin-1β (IL-1β), as well as tumor necrosis factor (TNF-α)] were measured. Conclusively, the application of BLM scaffold resulted in highest levels of best skin regeneration by increasing neovascularization and boosting collagen I/III deposition. Taken together, the 3D-printed BLM scaffolds can promote wound healing, and are highly suitable for a wide range of applications as wound dressings or skin substitutes.
研究领域:
生物医学工程
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组织工程
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再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Bio Materials
·
2025
·
IF 4.7
Kai Chen,Liu Luo,Ruolan Tao,Muzi Li,Shuqi Qu,Xiaofang Wu,Xinyue Zhang,Haiyan Feng,Ziqiang Zhu,Dekun Zhang
The application of a three-dimensional (3D)-printed biological functional scaffold in the repair of bone defects is a promising strategy. In this study, strontium-containing hydroxyapatite (SrHA) powder was synthesized by the hydrothermal method, and then poly(ε-caprolactone) (PCL)/HA and PCL/SrHA composite scaffolds were prepared by the high-temperature melt extrusion 3D printing technology. The basic physical and chemical properties, in vitro biological properties, osteogenesis, and angiogenesis abilities of the scaffold were studied. The results showed that HA and SrHA were uniformly embedded in the composite scaffold, and the scaffold exhibited a 3D interconnected porous structure and rough microsurface. The in vitro release curve showed that Sr2+ and Ca2+ were continuously released from the PCL/SrHA scaffold. In order to verify the performance of the composite scaffold in bone regeneration, the proliferation and osteogenic differentiation of mouse embryonic osteoblasts (MC3T3E1) grown on the scaffold were evaluated. The experimental results showed that the incorporation of SrHA significantly promoted cell proliferation. Compared with the PCL/HA scaffold, the PCL/SrHA scaffold could better promote cell osteogenic differentiation. Deferoxamine (DFO) was loaded on the surface of the PCL/SrHA scaffold. By studying the proliferation, angiogenesis, and expression of osteogenesis and angiogenesis-related genes of human umbilical vein endothelial cells (HUVECs) on PCL/SrHA@DFO scaffold, it was verified that DFO had the ability to promote angiogenesis. It could induce angiogenesis in vitro in combination with Sr2+. Therefore, we believe that the composite scaffold has potential application prospects in the field of bone tissue engineering.
研究领域:
生物材料学
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组织工程学
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医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Ming Jiang,Yuanzhong Pan,Yuanda Liu,Kai Dai,Qinghao Zhang,Jing Wang
Bone regeneration and vascularization have presented a clinical challenge for decades. Considering the importance of stem cells, such as mesenchymal stem cells (MSCs), in bone regeneration, endothelial progenitor cells (EPCs) are crucial during bone repair . This paper presented sulfated chitosan (SCS)-based hydrogel scaffolds to accelerate bone tissue regeneration , vascularization enhancement, and improve bone repair. Thus, these scaffolds played a crucial role in the regeneration of blood vessels, with the increased presentation of epithelial progenitors and immune cells in this microenvironment. In vivo experiments showed that the biological impact of SCS was critical for angiogenesis and vascularization, in conjunction with bone morphogenetic protein-2 (BMP-2) and MSCs. Therefore, the BMP-2-/hydrogel system established in this study promoted angiogenesis, stimulated MSC proliferation , and enhanced bone tissue formation. In addition, this paper highlighted the angiogenic role of SCS in creating a micro-environment for effective bone repair and provides insight into the future development of new bone regeneration material.
研究领域:
生物材料学
·
组织工程学
·
再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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