Analytical Methods
·
2017
·Vol. 9
·期 44
·6286-6292
·
IF 1.9
Lingxian Ye,Guangying Zhao,Wenchao Dou
Developing portable and low-cost methods for quantitative detection of Cronobacter sakazakii (C. sakazakii) from powdered infant formula plays a significant role in controlling and preventing a range of serious neonatal infections. Here, we reported a novel methodology for C. sakazakii detection using a low-cost, simple, and widely accessible personal glucose meter (PGM) as the readout. The electrochemical immunoassay strategy was based on antibody and glucose oxidase coated silica nanoparticles (SiNP–GOX–IgG) and antibody functionalized silica-coated magnetic nanoparticles (MNP–IgG). In the process of detecting a positive sample, a sandwich immunocomplex was formed between the MNP–IgG, C. sakazakii and SiNP–GOX–IgG. Then, the above sandwich composites were dispersed in glucose solution, and the concentration of glucose was measured using the PGM. Under optimal conditions, a linear relationship between the decrease of glucose concentration and the logarithm of C. sakazakii concentration was obtained in the concentration range from 9.0 × 102 to 9.0 × 107 CFU mL−1 with a detection limit of 4.2 × 101 CFU mL−1 (S/N = 3). This simple immunoassay holds great potential as an efficient, sensitive and selective point-of-care testing (POCT) tool for C. sakazakii detection.
研究领域:
微生物学
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分析化学
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医学诊断
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Xiaofang Liu,Qun Wang,Huan Liu,Yong Zhang,Mei Yang,Danqun Huo,Changjun Hou
Timely detection of breast cancer not only positively influences patient treatment and life quality, but also possesses far-reaching implications for public health and medical research. And miRNAs, as emerging breast cancer-related biomarkers, demonstrate heightened sensitivity to disease progression. In this study, the same precursor was utilized to prepare micromaterials with large and small cavities, this was the safeguard for sensing probes and signals. And the signal amplification process was accomplished with strand displacement assistance by target excitation. Encouragingly, it was successfully and accurately detected miRNA-21 and miRNA-155 within 10 fmol/L - 10 nmol/L. Due to the unique sensing design, the signal can be read out not only by small fluorescent devices, but also by portable glucometers. The biosensor features several excellent working performances and illuminates resource-poor areas by equipping the most common glucometers. So, it is a sensitive, portable and promising biosensor.
研究领域:
分子生物学
·
医学检测
·
生物传感技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2021
·
IF 6.057
Yuemeng Yang,Tingting Wu,Li-Ping Xu,Xueji Zhang
Staphylococcus aureus is one of the most important food-borne bacterial pathogens and causes numerous illnesses. In this work, we report a sensitive and highly selective magnetic-aptamer biosensor based on a personal glucose meter (PGM) platform for the detection of Staphylococcus aureus . The aptamer for Staphylococcus aureus was immobilized on the magnetic bead by hybridization with the capture probe P. In the presence of Staphylococcus aureus , the aptamer was dissociated from the magnetic bead. Then the capture probe was exposed and could be hybridized with a biotinylated probe to trigger the DNA hybridization chain reaction (HCR), thus achieving the signal amplification. The concentration of streptavidin-labeled invertase can be read by PGM, thus can lead to the portable quantitative detection of Staphylococcus aureus . After optimization of various conditions, 5 μM probe P, the MB-P reaction time for 36 h, the competition time for 60 min, 0.5 μM H1 & H2, 0.5 M sucrose and the sucrose invertase catalytic reaction time for 50 min was chosen to achieve the better sensor performance. Under the optimal conditions, the fabricated sensor offers high sensitivity with the limit of detection about 2 CFU/mL. This sensitive PGM based sensor could successfully evaluate the Staphylococcus aureus concentration in real food samples, and the results are consistent with those obtained by using plate counting methods. Moreover, the PGM sensor can greatly reduce the required time compared to the plate counting methods. The fabricated sensor supplies an ideal solution for rapid portable detection of bacterial pathogens and holds its potential use in the quality control for agriculture and food enterprises, entry-exit inspection and quality testing for food.
研究领域:
食品安全检测
·
生物传感技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ELECTROANALYSIS
·
2021
·Vol. 34
·期 2
·294-301
·
IF 3.223
Chang Wang,Jingyuan Yu,Lijuan Qi,Jiaxue Yu,Meiting Yang,Yan Du
We realized the user-friendly and field-based point-of-care detection on a self-made portable device with the aid of personal glucose meter (PGM) for single nucleotide polymorphisms (SNPs) of melanoma and colon cancer marker BRAF V600E with good sensitivity and selectivity by integrating the loop-mediated isothermal amplification (LAMP) reaction and the strand exchange signal transduction. The device is equipped with a rotating magnetic field as well as a heating element, under which the pre-added magnetic nanochains (MNCs) help to homogenize the reaction mixture and overcome the low diffusion efficacy of the reactant and the required temperature for different reactions can be met.
研究领域:
生物技术
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医疗器械
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癌症研究
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纳米技术
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分子诊断
✓ 已验证
产品关联
DOI
高影响因子
Biosensors-Basel
·
2022
·Vol. 12
·期 12
·1084
·
IF 5.743
Tao Tian,Wei-Yi Zhang,Hang-Yu Zhou,Li-Jing Peng,Xi Zhou,Hao Zhang,Feng-Qing Yang
In this study, the personal glucose meter (PGM) was first used as a fast and user-friendly meter for analyzing catechol (CA) based on the reduction of the mediator K3[Fe(CN)6] to K4[Fe(CN)6] in the glucose test strip. Then, an easy, low-cost, and convenient PGM-based method for detecting tyrosinase (TYR) activity and sodium benzoate (SBA) was developed on the basis of the TYR-catalyzed reaction. In this method, CA is oxidized to formo-benzoquinone by TYR, thereby reducing the residual amount of CA and the PGM readout. On the other hand, SBA can inhibit the oxidation of CA catalyzed by TYR and increase the residual amount of CA after the enzymatic reaction. Therefore, the activity of TYR is proportional to the difference in the PGM readout of CA, and the concentration of SBA is positively correlated with the residual amount of CA. After the relevant experimental conditions were systematically optimized, the proposed PGM-based method for the detection of TYR and SBA was successfully validated. The liner ranges are 1.0–103.3 U/mL and 6.25–1000 ppm, and the quantification limits are 1.0 U/mL and 6.25 ppm for TYR and SBA, respectively. Moreover, the spiked recovery tests in normal human serum and carbonate beverages (i.e., Cola, Sprite, and Fanta) were performed, and the recoveries (91.6–106.8%) further confirm the applicability of the PGM-based method in real sample analysis.Keywords:tyrosinase;sodium benzoate;personal glucose meter;enzyme sensor;carbonate beverages sample
研究领域:
分析化学
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生物化学
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Lilin Ge,Bin Li,Houxi Xu,Wenyuan Pu,Hang Fai Kwok
A simple and feasible electrochemical immunosensing protocol with glucometer readout was designed for the detection of low-abundance disease-related biomarker (alpha-fetoprotein; AFP) on the basis of backfilling rolling cycle amplification (RCA) with invertase-DNA 2 conjugates on the detection antibody. The assay consisted of the immunoreaction, RCA reaction, DNA 2 -invertase hybridization and glucose measurement. Initially, a sandwiched immunoreaction was carried out between anti-AFP capture antibody-coated microplate between nanogold-labeled pAb 2 detection antibody conjugated with DNA 1 primer (DNA 1 -AuNP-pAb 2 ) in the presence of target ATP . Thereafter, the carried primers triggered the RCA reaction in the presence of circular DNA template, polymerase and dNTP, to produce numerous repeated oligonucleotide sequences for hybridization with many invertase-DNA 2 conjugates. The carried invertase molecules accompanying the hybridization reaction hydrolyzed sucrose into glucose, thereby resulting in the amplification of the detectable signal on a handheld personal glucometer (PGM). Under optimum conditions, the developed immunoassay exhibited high sensitivity for the quantitative screening of AFP within a dynamic range of 0.1–100 ng mL −1 at a low detection limit of 0.087 ng mL −1 . Other biomarkers and proteins did not interfere the signals of this system. In addition, this method was utilized to determine human serum samples containing target AFP, and received well-matched results with the referenced enzyme-linked immunosorbent assay (ELISA) method.
研究领域:
生物技术
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医学诊断
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电化学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2017
·Vol. 409
·期 17
·4139-4147
·
IF 3.431
Luo Yiheng,Dou Wenchao,Zhao Guangying
In this article, a facile and sensitive electrochemical method for quantification of Salmonella Pullorum and Salmonella Gallinarum ( S. Pullorum and S. Gallinarum) was established by monitoring glucose consumption with a personal glucose meter (PGM). Antibody-functionalized magnetic nanoparticles (IgG-MNPs) were used to capture and enrich S. Pullorum and S. Gallinarum, and IgG-MNPs- S. Pullorum and IgG-MNPs- S. Gallinarum complexes were magnetically separated from a sample using a permanent magnet. The trace tag was prepared by loading polyclonal antibodies and high-content glucose oxidase on amino-functionalized silica nanoparticles (IgG-SiNPs-GOx). With a sandwich-type immunoassay format, IgG-SiNPs-GOx were added into the above mixture solution and conjugated to the complexes, forming sandwich composites IgG-MNPs/ S. Pullorum and S. Gallinarum/IgG-SiNPs-GOx. The above sandwich composites were dispersed in glucose solution. Before and after the hydrolysis of glucose, the concentration of glucose was measured using PGM. Under optimal conditions, a linear relationship between the decrease of glucose concentration and the logarithm of S. Pullorum and S. Gallinarum concentration was obtained in the concentration range from 1.27 × 10 2 to 1.27 × 10 5 CFU mL −1 , with a detection limit of 7.2 × 10 1 CFU mL −1 (S/ N = 3). This study provides a portable, low-cost, and quantitative analytical method for bacteria detection; thus, it has a great potential in the prevention of disease caused by S. Pullorum and S. Gallinarum in poultry.
A schematic illustration of the fabrication process of IgG-SiNPs-GOD nanomaterials ( A ) and IgG-MNPs ( B ) and experimental procedure of detection of S. Pullorum and S. Gallinarum using GOD-functionalized silica nanospheres as trace tags based on PGM ( C ).
研究领域:
微生物学
·
免疫学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ELECTROANALYSIS
·
2021
·Vol. 34
·期 2
·246-251
·
IF 3.223
Ling Li,Dong Liang,Wuhua Guo,Dianping Tang,Yongyi Zeng
Methods based on immunoassays have been developed for cancer biomarker alpha-fetoprotein (AFP), but most involve complicated or stripping procedures and are unfavourable for routine use. Herein we report the proof-of-concept of simple and point-of-care (POC) immunoassay for AFP in hepatocellular carcinoma on a portable personal glucometer (PGM) by using antibody-invertase cross-linkage nanoparticles as the signal-generation tags. Antibody-invertase cross-linkage nano tags were synthesized by using reverse micellar method with glutaraldehyde. The POC immunoassay was carried out on monoclonal anti-AFP capture antibody-coated microplate with a sandwich-type reaction mode. Introduction of invertase nano labels accompanying target AFP could hydrolyse sucrose into glucose and fructose. The produced glucose molecules could be determined on a portable personal glucometer. Relative to unimolecular invertase labelling, improved analytical features were acquired with antibody-invertase nano labelling. With the nano labels, PGM-based immunoassay exhibited good electrochemical responses for the detection of AFP, and allowed detection of AFP at a concentration as low as 5.4 pg mL −1 . Moreover, a good repeatability and intermediate precision could be found down to 12.68 %. Good well-matched results were obtained for analysis of human serum specimens between POC immunoassay and commercial human AFP ELISA kit.
研究领域:
生物技术
·
医学诊断
·
纳米技术
·
肿瘤学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2022
·
IF 6.556
Biyun Fang,Zhenzhen Jia,Cui Liu,Kangsheng Tu,Mingzhen Zhang,Lei Zhang
Pathogen nucleic acid detection is of great significance to control the spread of diseases caused by the viruses. Nevertheless, traditional methods for nucleic acid detection such as polymerase chain reaction (PCR) and oligonucleotide microarrays require bulky instruments, which restrain their point-of-care (POC) testing application. Here, we proposed a POC method enabling sensitive detection of pathogen nucleic acids by combining the clustered regularly interspaced short palindromic repeat (CRISPR) Cas12a-based assay and personal glucometer readout (PGM). The quantification of target pathogen DNA by PGM was achieved based on pathogen DNA activates Cas12a ssDNase to cleave magnetic bead-DNA-invertase reporter probe, and separated free invertase to catalyze hydrolysis of sucrose to glucose. Without using nucleic acid amplification technology, we demonstrated here dual signal amplifications based on Cas12a and invertase-mediated catalytic reactions, making it possible to sensitively detect HIV-related DNA or SARS-CoV-2 pseudovirus with the limits of detection of 11.0 fM and 50 copies/μL, respectively. This strategy also showed excellent selectivity as well as potential applicability for detection of HIV in human serum samples or of SARS-CoV-2 in saliva samples. Therefore, our CRISPR-PGM-based dual signal amplifications detection platform might offer a great promise in POC diagnosis of pathogen nucleic acids.
研究领域:
分子生物学
·
医学诊断
·
生物技术
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Yayun An,Dafeng Jiang,Nan Zhang,Wei Jiang
Amyloid β oligomer (AβO) is an important biomarker for Almerzheimer's disease (AD) early diagnosis. In present study, cascade primer exchange reaction (PER) based amplification strategy was proposed for sensitive and portable detection of AβO using personal glucose meters (PGM). Two PER processes were employed here. In the primary PER, the hairpin template 1 (HT1) was designed with a primer binding domain, a primer extending domain and a blocking extending domain. The primers were designed to be modified on magbeads surface. Initially, the primer binding domain in HT1 was locked by AβO aptamer . When target AβO was present, aptamer bound with AβO and dissociated from HT1 to initiate the primary PER. The products acted as the primer of the secondary PER to hybridize with another hairpin template 2 (HT2), initiating the secondary PER and producing numerous ssDNA with repeated DNA-invertase binding sites. After binding with DNA-invertase, the obtained conjugates were magnetically separation to catalyze the conversion of sucrose to glucose, which were detected by a PGM. The strategy achieved a limit of detection of 0.22 pM with a linear ranged from 1 pM to 250 pM. Satisfactory reproducibility results were obtained in actual samples. This strategy provided a superior tool for sensitive and convenient detection of AβO, and showing a great potential in the early diagnosis of AD.
研究领域:
生物化学
·
生物技术
·
诊断学
·
神经退行性疾病
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Aggregate
·
2024
·
IF 13.9
Yi Long,Shurui Tao,Dongni Shi,Xingyu Jiang,Tian Yu,Yingxi Long,Libing Song,Guozhen Liu
Developing the sensitive point-of-care testing (POCT) of oncogenic nucleic acids from human papillomavirus (HPV) infection is essential in preventing cervical cancer, especially in resource-limited settings. Rolling circle amplification (RCA) is attractive in achieving POCT via nucleic acid-based aggregation under isothermal conditions. However, the influence of RCA product structure on the aggregation remains unexplored resulting in limited sensitivity. Here, a minimum secondary structured RCA technique (MSS-RCA) is developed by designing a unique circular template, demonstrating significantly enhanced detection sensitivity with only one amplification step and one primer under isothermal conditions. The amplification efficiency of MSS-RCA could be kinetically manipulated by controlling the secondary structure of the circular template. Introducing the invertase probe to MSS-RCA, HPV16 E6/E7 nucleic acid target was detected with a personal glucose meter (PGM) with a sensitivity of 5 f m (50 zmol in 10 µL). This integrated MSS-RCA-PGM detection system was successfully applied to detect HPV16 E6/E7 mRNA extracted from 54 cervical swab samples reaching a positive predictive value of 100.00% and negative predictive values of 96.00% (77.77% to 99.40%, 95% CI). MSS-RCA-PGM provides a sensitive POCT platform for the detection of nucleic acid biomarkers for screening of cervical cancer or other diseases.
研究领域:
分子生物学
·
癌症筛查
·
即时诊断
·
核酸扩增
✓ 已验证
产品关联
DOI
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2022
·
IF 3.705
Tao Tian,Hao Zhang,Feng-Qing Yang
A rapid, portable, and cost-effective method using personal glucose meter (PGM) for quantitative analysis of hydrogen peroxide (H 2 O 2 ) was established based on ascorbate oxidase (AAO)-catalyzed reaction for the first time. Ascorbic acid (AA) can rapidly reduce ferricyanide (K 3 [Fe(CN) 6 ]) to ferrocyanide (K 4 [Fe(CN) 6 ]) in the glucose test strip and transfer electron to the electrode to generating a PGM detectable signal. Thus, the concentration of AA can be directly determined by the PGM as simple as measuring the blood glucose. On the other hand, AAO can catalyze the reduction of H 2 O 2 and produce an enzyme-peroxide complex, which decreases the yields of dehydroascorbic acid formed by the oxidation of AA, resulting in the increase in PGM detectable signal of residual ascorbic acid (re-AA). Therefore, the concentration of H 2 O 2 is proportional to the concentration of re-AA. After optimization of the experimental conditions, the developed method can be used to detect H 2 O 2 at linear range of 2.5–5 × 10 3 μM with the quantification limit of 2.5 μM. In addition, the satisfactory spiked recoveries (95.3–108.9 %) of real samples (i.e., tap water, contact lens solution, medical hydrogen peroxide, and normal human serum) confirm its feasibility for practical applications. In short, this study provides a feasible PGM-based method for H 2 O 2 detection with simple operations.
研究领域:
分析化学
·
生物化学
·
环境科学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）