JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2018
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IF 3.235
Yang Yuan,Jiaxin Wang,Xinjiong Ni,Yuhua Cao
A biosensor for determination of 3-chloro-1, 2-propandiol (3-MCPD) was developed based on the electrochemically catalytic reduction by hemoglobin (Hb). Hb was concentrated and immobilized with magnetic molecularly imprinted nanoparticles (MMIPs NPs). The MMIP NPs had a core-shell structure, with magnetic Fe 3 O 4 NPs as the core and SiO 2 as the shell. MMIPs NPs were modified on the surface of a magnetic electrode to constitute the Hb enzyme-like sensor. The biosensor could catalyze the electrochemical reduction of 3-MCPD, and a direct electrochemical determination of 3-MCPD could be achieved for the first time. The biosensor, where Hb immobilized with MMIPs NPs had high electrochemical response for 3-MCPD, increased by 37.5% compared with Hb immobilized with magnetic non-imprinted nanoparticle (MNIPs NPs). The biosensor increased the response by 19.56% again under an external magnetic field . The reduction peak current had a linear relationship with 3-MCPD concentration ranging from 1.0 mg·L −1 to 160 mg·L −1 with a detection limit (S/N = 3) of 0.25 mg·L −1 . The biosensor was applied to the determination of 3-MCPD in soy sauce samples successfully with recoveries between 97.4% and 99.6%.
研究领域:
生物传感器
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分析化学
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食品科学
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电化学
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分子生物学
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纳米医学
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COLLOIDS AND SURFACES B-BIOINTERFACES
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2025
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IF 5.6
Fang Zhao,Wukun Zhong,Chenyu Ding,Han Xiao,Tong Lv,Qitian Lin,Xiao Fang,Chunsen Li,Dezhi Kang,Chunhua Lu
Carbon monoxide (CO) is a gaseous signaling molecule, which can induce mitochondrial dysfunction and tumor cell apoptosis by increasing reactive oxygen species (ROS), making it an attractive therapeutic agent for tumors. However, achieving the optimal therapeutic dose of CO is often difficult due to the limited drug loading capacity of delivery systems. Moreover, the CO therapeutic effect is compromised by the overexpression of glutathione (GSH) in tumor cells. Here, we report a nanodrug constructed by self-assembly of CO-releasing molecule Mn(CO) 5 Br and Cy5-modified cystine transporter antisense oligonucleotides. This nanodrug dissociates and releases CO and antisense oligonucleotide in response to overexpressed H 2 O 2 in tumor microenvironment, resulting in the increase of ROS and the decrease of GSH respectively, thus synergistically inducing tumor cell apoptosis. The self-assembled nanodrug shows satisfactory therapeutic effect of inhibiting tumor growth in tumor cells and animal models, and provide a promising strategy for multifunctional anti-tumor synergistic therapy.
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ELECTROANALYSIS
·
2026
·Vol. 38
·期 1
·e70098
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IF 2.3
Qiu Jin,Xuyuan Chen,Xiaoying Wang
Excessive oligomeric Amyloid- β (A β Os) in the brain induces strong neurotoxicity and are a major causative marker of Alzheimer's disease (AD). Herein, an exemplary electrochemiluminescence (ECL) aptasensor for A β Os that coupled a triple-helix switchable system (THSS) with gold-doped Ru(bpy) 3 2+ /copper luminescent-nanospheres (RuCu@AuLNs) was successfully developed. In brief, the anti-A β Os aptamer (Apt) with two symmetric arms and RuCu@AuLN-marked signal transduction DNA (RuCu@AuLNs-ST) perfectly constructed the THSS on a gold electrode surface via hybridization reaction. At this point, the THSS was closed, and a strong initial ECL signal ( I ECL0 ) output was generated. Once A β Os appeared, they specifically bound with Apt and opened the THSS, releasing RuCu@AuLNs-ST from electrode surface, and degressive ECL signal ( I ECL1 ) was obtained. Thus, the aptasensor detected A β Os-dependent I ECL1 change (Δ I ECL1 = I ECL0 − I ECL1 ) with a concentration range of 1 fM to 1 pM with a low detection limit of 0.5 fM, and demonstrated good reliability and practicality in human serum. Additionally, the reconfigurability of the sensor allows it to be continuously reused up to 5 times at a concentration of 1 fM A β Os. The developed ECL aptasensor could be used as a cost-effective tool for early diagnosis of AD. Moreover, the strategy has promising application in the bioassays of various analytes.
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CARBOHYDRATE POLYMERS
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2026
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IF 12.5
Yuxuan Yang,Sufeng Zhang,Lijing Zhang,Nan Zhang,Liwei Qian,Wenqi Song
To address the low binding-site density and mass-transfer limitations of conventional protein-molecularly imprinted polymers (P-MIPs), this study proposes a synergistic strategy combining a custom-synthesized imidazolium ionic liquid monomer, 1-vinyl-3-diacetamidoimidazolium chloride ([VDAIM]Cl), with a bacterial-cellulose@UiO-66 (BC@UiO-66) hybrid scaffold. Spectral characterization and molecular docking simulations revealed multiple strong interactions between [VDAIM]Cl and bovine serum albumin (BSA), enhancing the stability of the pre-polymer complex compared to conventional monomers. Meanwhile, benefiting from the structural advantages of BC, the template immobilization-surface imprinting strategy (TIS- co -SIT) was employed to construct a high-affinity imprinted membrane. The resulting BC@UiO-66@MIPs exhibited fast adsorption equilibrium within 50 min, a large binding capacity of 502.5 ± 27 ( n = 3) mg g −1 , and a high imprinting factor ( IF ) of 6.27 ± 0.84 (n = 3). Furthermore, BC@UiO-66@MIPs also demonstrated excellent reusability, retaining over 90% of its initial binding capacity after ten adsorption-desorption cycles. Notably, the materials exhibited outstanding selectivity, with a competitive adsorption factor ( α ) of 5.57 ± 0.62 ( n = 3) in binary BSA/BHb mixtures, and a high permeation selectivity coefficient ( β ) of 6.37. The membrane successfully isolated BSA from complex calf serum, confirming its practical potential for protein purification. This study constructs an efficient molecular imprinting system by integrating rational monomer design with advanced porous supports to enhance protein recognition and purification.
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Exploration
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2026
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IF 30.4
Liang Ma,Hongchuan Wang,Weidong Liang,Tao Xu,Jie Lei,Li Zhang,Shutao Gao,Xiaoyu Cai,Qian Li,Wenzhao Wang,Chuanhui Xun,Weibin Sheng,Cao Yang,Yingsong Wang
Repairing infectious tissues, particularly bone and wound infections, poses significant challenges to clinicians due to the lack of effective treatment modalities. Sonodynamic therapy, which utilizes sonosensitizers to generate reactive oxygen species (ROS) under ultrasound (US) energy for antibacterial purposes, still lacks suitable sonosensitizers with good biocompatibility that can simultaneously promote the repair of various infected tissues. In this study, we constructed a novel sonosensitizer composed of hemoglobin derived from nature and zeolitic imidazolate framework (ZIF)-8, which exhibits excellent biocompatibility and activity. This sonosensitizer enables early-stage high-power US to generate substantial ROS for antibacterial effects and late-stage low-power sono-ionic therapy for tissue repair. We employed bone, wound infection, and wound biofilm models to evaluate the therapeutic efficacy in different infected tissues. We utilized microneedles to assemble nanoparticles to match the different disease models, achieving deep biofilm clearance. Furthermore, to investigate the repair mechanisms, we developed a bioadhesive-derived hydrogel loaded with nanoparticles for wound treatment. RNA sequencing revealed that sono-ionic therapy promotes tissue repair through the peroxisome proliferator-activated receptor signaling pathway. Therefore, our platform holds great potential for broad-spectrum treatment of infectious tissue repair.
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Advanced Science
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2026
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IF 14.1
Yan Xu,Weitao Yang,Yanjing Yun,Hui Wang,Zhuoyao Wu,Youyi Yu,Bingbo Zhang
Non-invasive visualization of glucose metabolism in living organisms remains a major challenge, as existing techniques cannot specifically detect glucose molecules with deep-tissue penetration and without ionizing radiation. To overcome this, we developed an enzyme-activated magnetic resonance imaging strategy (eaMRI) using a biodegradable nanoprobe, CrGOx@Lip, that integrates endogenous Cr 3+ ions with glucose oxidase for glucose-responsive imaging. Our approach leverages GOx not only as a catalytic engine to specifically oxidize glucose but also as a structural template to guide the in-situ synthesis of a potent MRI reporter (paramagnetic chromium gluconate). This design enables direct, specific amplification of MRI signals in proportion to local glucose concentration, achieving an 8.28-fold relaxivity increase. We validated this method for sensitive glucose mapping in vivo, including delineating Warburg-effect-driven tumors and quantifying pathological glucose accumulation in metabolic dysfunction-associated fatty liver disease (ΔSNR% = 18.51 ± 3.72 vs. 1.14 ± 1.39 in controls; p <0.001), and further demonstrated its utility in monitoring therapeutic efficacy via glucose-responsive signal changes. By synergizing enzymatic precision with nanomaterial engineering, our CrGOx@Lip-mediated eaMRI platform provides a glucose-specific, radiation-free, and non-invasive strategy for sensitive metabolic diagnostics in precision medicine.
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CARBON
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2026
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IF 12.7
Xinyu Yang,Xiaorui Yang,Dan Zhao,Rong Wang,Xincai Xiao
The excellent optical properties, enzyme-mimicking activities and unique surface functional groups of carbon dots (CDs) offer promising potential for fluorescent/colourimetric dual-mode detection of Hg 2+ . This paper reports the preparation of yellow-green fluorescent carbon dots (Fe-CDs) with outstanding peroxidase (POD) and superoxide dismutase (SOD) activities via a one-step hydrothermal method, achieving a quantum yield of 20.6%. Kinetic analysis revealed that the Michaelis constants ( K m ) of the Fe-CDs’ POD-like activity for OPD and H 2 O 2 were 0.15 mM and 2.36 mM, respectively. Furthermore, Fe-CDs demonstrated SOD-like activity, with half-maximal inhibitory concentration (IC 50 ) values of 0.1 mg/mL for O 2 •− and 4 μg/mL for DPPH radicals. Fe-CDs were also capable of reacting with ABTS radical cations (ABTS •+ ), generating a purple product with a characteristic absorption peak at 540 nm. Based on the inhibitory effect of Hg 2+ on the enzymatic activity of Fe-CDs—which reduces the formation of ABTS •+ , thereby restoring fluorescence intensity and causing the fading of the solution's colour—a dual-channel fluorescent/colourimetric detection platform was developed. This study not only achieved the synthesis of CDs with multi-enzyme activities but also established a selective detection method.
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BME Frontiers
·
2026
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IF 9.1
Xingfang Qiao,Yiwen Hao,Yinglan Yu,Zhe Qiang,Xingjian Wen,Yanlei Guo,Zhen Wu,Hao Shao,Lei Luo
Objective: This study aims to engineer a tigecycline–hemoglobin nanoparticle (TIG–HBNP) system to enhance the targeting effect of tigecycline (TIG) on Klebsiella pneumoniae , thereby improving its antibacterial efficacy. Impact Statement: This study represents the first report of loading TIG onto hemoglobin (HB) nanoparticles. This system utilizes the iron dependence of K. pneumoniae to enhance the targeting ability of TIG and increase the drug concentration at the infection site, thereby enhancing the antibacterial activity and providing a promising strategy for combating drug-resistant gram-negative bacterial pneumonia. Introduction: K. pneumoniae is a gram-negative bacillus that causes severe primary pneumonia with high pathogenicity and increasing drug resistance. TIG is a key therapeutic option, but its clinical effectiveness is limited by extensive systemic distribution and insufficient drug concentration at infectious foci. HB serves as a promising protein nanocarrier and provides iron, an essential nutrient for K. pneumoniae growth, suggesting great potential for targeted antibiotic delivery. Methods: Molecular docking was performed to analyze the binding affinity and interaction between HB and TIG. TIG–HBNP was fabricated via a drug–protein self-assembly approach. The nanoparticles were characterized in terms of particle size, zeta potential, drug-loading efficiency, morphology using transmission electron microscopy and atomic force microscopy, stability, and biocompatibility. Targeting capability was evaluated. Pharmacokinetic profiles and antibacterial activity were further assessed. Results: Molecular docking verified stable binding between TIG and HB. TIG–HBNP exhibited a uniform spherical morphology, a particle size of approximately 200 nm, a negative surface charge, and a drug-loading efficiency exceeding 20%. The nanoparticles showed favorable stability and safety. Enhanced targeting to K. pneumoniae was confirmed in both in vitro and in vivo models. Improved pharmacokinetic behavior and enhanced antibacterial activity against K. pneumoniae were also observed. Conclusion: TIG–HBNP enhances TIG’s therapeutic efficacy against K. pneumoniae infections. Furthermore, HB holds promise as a versatile carrier for diverse antibiotics, offering a scalable platform to combat multidrug-resistant pathogens.
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ACS Applied Polymer Materials
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2025
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IF 4.7
Huanping Wu,Xiaoqiang Wu,Xuyang Liu,Zulpiyam Ahmatjan,He Teng,Chenchen Zhang,Jianlong Ye,Jianqiang Meng
The preparation of molecularly imprinted polymers by simple and universally applicable methods for the rapid and efficient purification of proteins has always been a significant challenge within the realm of biological separation. In this work, interconnected porous molecularly imprinted polyampholyte hydrogels (IPMIPs) were prepared by one-step free radical polymerization of monomers bearing opposite charges by using high internal phase emulsions as templates for lysozyme (Lyz) separation. By changing the concentration of surfactant Tween 20 and hydrophilic silica nanoparticles N20, the pore interconnectivity and pore size of the imprinted hydrogels could be finely adjusted, thereby affecting the swelling process. Owing to the well-defined and interconnected porous structure, IPMIPs were capable of achieving adsorption equilibrium within a remarkably short time frame of 45 min. The monolayer adsorption capacities of IPMIPs for Lyz reached an impressive 242.5 mg/g with an imprinting factor (IF) of 3.01 for Lyz. Through electrostatic complementation and hydrogen bond interactions, IPMIPs showed Lyz adsorption capacities significantly higher than those of other reference proteins, and the selectivity coefficient (α) to ovalbumin (OVA) was as high as 7.17. The real sample separation and regeneration experiments further demonstrated the excellent specific recognition ability and reliability of the IPMIPs. This strategy presents a highly promising alternative for the Lyz separation process and has the potential to be extended to other protein molecules.
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Journal of Materials Chemistry B
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2025
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IF 5.7
Siyue Wang,Xiaotian Guan,Shuqing Sun
C-reactive protein (CRP) is a well-established biomarker whose plasma levels increase significantly during inflammatory and infectious episodes. It plays a vital role in the diagnosis of bacterial infections, autoimmune diseases, and cardiovascular risk assessment. However, conventional detection techniques often struggle to balance high sensitivity with a broad dynamic range. Here, we developed and validated a novel fluorescence-based aptasensor named AuCA (AuNP-enhanced CRISPR Aptasensor), which integrates nucleic acid aptamers, magnetic beads (MBs), gold nanoparticles (AuNPs), and the CRISPR/Cas12a system for robust CRP quantification. In this system, MBs functionalized with aptamer 1 selectively captured the target protein, while AuNPs co-modified with aptamer 2 and a Cas12a activation sequence (Trigger) enabled efficient signal transduction. Upon target binding, the Trigger activated the Cas12a/crRNA complex, initiating trans-cleavage of fluorescent reporters and producing a markedly amplified signal. The optimized AuCA platform achieved a low detection limit of 60 ng/mL and a quantifiable range of 0.1 -150 µg/mL. It exhibited excellent specificity and resistance to biological interference, ensuring reliable measurements even in complex sample matrices. When applied to clinical human plasma, AuCA demonstrated strong concordance with results from a commercial immunoturbidimetric assay. AuCA allowed the simultaneous detection of both standard and hypersensitive CRP (hsCRP), supporting comprehensive full-range CRP (frCRP) analysis with strong potential for clinical application.
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BIOSENSORS & BIOELECTRONICS
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2025
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IF 10.5
Zhenjie Zhang,Wei Wei,Anne Ailina Li,Chenhao Wang,Chengchen Tang,Qing Wang,Lianqun Zhou,Hui Kong
Here, we report a wearable device integrating a sweat collection unit and a molecularly imprinted polymer (MIP) - based sensor for the non-invasive detection of adrenaline (AD). In this paper, a new MIP method is proposed, which uses AD molecule as template, and electropolymerizes aniline and 3-aminophenylboronic acid (3-APBA) to form molecular imprinting. Surface modifications, incorporating carbon nanotubes (CNT) and MIP optimization, enhanced the device's specific surface area by 166.45% (± 2.82%), yielding a wide linear detection range of AD (10 -6 μmol L -1 to 10 3 μmol L -1 ). In this study, a three-dimensional response model was constructed by analyzing the current values corresponding to sweat pH levels (4.0-8.0) and AD concentrations, enabling a linear response and accurate prediction of AD levels in sweat. The integrated flexible electronic platform supports real-time data transmission via Bluetooth or NFC to a mobile application, facilitating efficient detection. Comprehensive integration and validation, including artificial sweat testing and correlation with ELISA results, underscore the sensor’s potential as a robust and versatile solution for wearable health monitoring.
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ANALYTICAL CHEMISTRY
·
2026
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IF 7.3
Qingfei He,Lingjun Cheng,Yuanyuan Yang,Zhiyan Zou,Canping Su,Xuan Shi,Jiaming Chen,Weirong Huang,Zhenyu Lin,Guolin Hong
Gold nanoclusters (AuNCs) are emerging electrochemiluminescence (ECL) emitters with unique luminescence and high stability. However, conventional AuNC-based ECL systems, which rely on single-signal regulation, often exhibit weak signal responses and limited stability. To address the challenge of detecting extremely low concentrations of the Alzheimer’s disease (AD) blood biomarker Aβ40, this study presents a novel ECL-based biosensing platform that leverages dual-signal regulation of AuNCs via glutathione (GSH) and H2O2 serving as ECL quenchers. Based on the peroxidase-like activity of the Aβ40–hemin complex, this system restores the ECL signal by catalyzing the consumption reaction between GSH and H2O2. Using this mechanism, a dual-regulation ECL platform was developed, demonstrating a linear detection range of 0.001–100 ng/mL for Aβ40 with a detection limit of 0.18 pg/mL (S/N = 3). This approach not only offers high sensitivity and specificity but also reduces the complexity of surface modification steps. Overall, this study advances ECL technology through dual regulation and provides a sensitive, practical, efficient, and reliable analytical method for early AD screening.
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