Water
·
2025
·Vol. 17
·期 3
·392
·
IF 3
Jing Dai,Bei Song,Ruyi Sha,Zhenzhen Wang,Jianwei Mao
Different pollutants often coexist in natural environments, making it crucial to monitor and study the ecotoxicological effects of composite pollutants in aquatic environments. Nanoplastics and heavy metals are emerging environmental pollutants that can affect the health of aquatic organisms and threaten human health via the food chain. In this study, zebrafish was employed as a model organism to explore the effects of short-term exposure to polystyrene nanoplastics (PS-NPs) and heavy metal copper ions (Cu2+) either individually or in combination on fish behavior. First, the single and combined toxicity of Cu2+and PS-NPs to adult zebrafish was investigated to obtain the LC50values of the two pollutants at 24, 48, 72, and 96 h. Then, the effects of sub-lethal concentrations of Cu2+(0.06, 0.15, and 0.3 mg/L), PS-NPs (5, 10, and 15 mg/L) and binary mixtures containing Cu2+and PS-NPs (0.06 mg/L + 10 mg/L, 0.15 mg/L + 10 mg/L, and 0.3 mg/L + 10 mg/L) on the swim speed and individual distance of zebrafish within 4 h were studied. The results show that the LC50value for single exposure of zebrafish to Cu2+decreased with the increase in the exposure time, while PS-NPs showed no significant acute toxicity to zebrafish when the concentration was less than 20 mg/L and the exposure time was less than 96 h. The combined exposure of zebrafish to Cu2+and PS-NPs resulted in a 3.1–32.2% reduction in the LC50value at different time points compared with Cu2+alone. In the behavioral study, both single and combined exposure to Cu2+and PS-NPs induced hyperactivity and aggregation phenomena in the zebrafish at different levels; the duration of these two phenomena was correlated with the concentration of the pollutants. The combined exposure to Cu2+and PS-NPs exacerbated the behavioral changes in zebrafish compared with exposure to Cu2+alone, reducing their hyperactivity time, average swim speed and aggregation time by 30.7–41.0%, 13.6–15.4%, and 28.3–28.8%, respectively. Therefore, this study indicates that the combined short-term exposure to PS-NPs and Cu2+can exacerbate the toxicity of pollutants, and also proves the feasibility for early warning of combined NPs and heavy metals pollution based on adult zebrafish behavioral indicators.
研究领域:
环境科学
·
生态毒理学
✓ 已验证
产品关联
DOI
高影响因子
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
·
2024
·
IF 3.9
Jie Tang,Anqi Liu,Kun Chen,Yanhong Shi,Xuchun Qiu
Amitriptyline (AMI), one of the widely used tricyclic antidepressants (TCAs), has become a pharmaceutical contaminant frequently detected in aquatic ecosystems. However, the impacts of AMI exposure and underlying mechanisms on fish are still limited. In this study, adult zebrafish (F0) were exposed to AMI at 0 (control), 0.8, and 8 μg/L for 14 days. Subsequently, the exposed zebrafish were paired for spawning, and their offspring (F1) were reared in an AMI-free medium until 5 days post-fertilization (dpf). This study aimed to assess variations in behaviors and neurotransmitter levels in both the F0 (at the end of the 14-day exposure) and F1 generations (at 5 dpf). As a result, waterborne AMI exposure significantly reduced the locomotor activity, frequency of body contact, and duration of chase in F0 zebrafish, and resulted in notable changes in monoamine neurotransmitter levels in their brains. Parental exposure to AMI significantly elevated the heart rate and eye movement but reduced the locomotor activity in the F1 zebrafish, also along with significant changes in monoamine neurotransmitters and acetylcholine. Furthermore, significant correlations between the changes in behavioral traits and neurotransmitter levels were identified in both F0 and F1 generations. Our findings confirm the critical role of monoamine modulation in the neurobehavioral toxicity of AMI on zebrafish and their offspring, and emphasize the importance of paying attention to its multigenerational effects on fish.
研究领域:
水生毒理学
·
药理学
·
神经科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nutrients
·
2024
·Vol. 16
·期 18
·3048
·
IF 4.8
Zhe Chen,Naiyan Lu,Xu Li,Qingrun Liu,Yujie Li,Xiyue Li,Ximiao Yu,Haotian Zhao,Chang Liu,Xue Tang,Xun Wang,Weisun Huang
Nicotine dependence is an important cause of excessive exposure to tobacco combustion compounds in most smokers. Nicotine replacement therapy is the main method to treat nicotine dependence, but it still has its shortcomings, such as the inability to mitigate withdrawal effects and limited applicability. It has been hypothesized that a combination of low-dose nicotine and caffeine could achieve the same psychological stimulation effect as a high dose of nicotine without causing nicotine withdrawal effects. To establish a model of nicotine dependence, male C57BL/6J mice were subcutaneously injected four times a day with nicotine (2 mg/kg) for 15 days and fed with water containing nicotine at the same time. They were randomly divided into four groups. After 24 h of withdrawal, different groups were injected with saline, nicotine (0.25 mg/kg or 0.1 mg/kg), or nicotine (0.1 mg/kg) and caffeine (20 mg/kg). Behavioral and physiological changes were evaluated by an assessment of physical signs, open field tests, elevated plus maze experiments, forced swimming tests, hot plate tests, and new-object-recognition tests. The changes in dopamine release in the prefrontal cortex (PFC) and ventral tegmental area (VTA) in the midbrain were analyzed using ELISA. The results showed that a combination of caffeine and nicotine could effectively relieve nicotine withdrawal syndrome, increase movement ability and pain thresholds, reduce anxiety and depression, enhance memory and cognitive ability, and increase the level of dopamine release in the PFC and VTA. Thus, caffeine combined with nicotine has potential as a stable and effective treatment option to help humans with smoking cessation.
研究领域:
药理学
·
神经科学
·
行为科学
·
成瘾医学
✓ 已验证
DOI
PubMed
高影响因子
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