LUMINESCENCE
·
2021
·Vol. 36
·期 7
·1584-1591
·
IF 2.464
Yaning Qin,Zhaoyue Yan,Rui Liu,Chaojie Qu,Haichen Mao,Lingbo Qu,Ran Yang
In this work, novel types of nitrogen-doped carbon dots (N-CDs) were prepared from citric acid and glycine (GLY) as precursors through a simple pyrolysis method. The GLY-CDs showed strong fluorescence with a fluorescence quantum yield as high as 33.34% and good water solubility. The fluorescence of GLY-CDs could be selectively quenched by iron(III) ion (Fe 3+ ) resulting in the non-fluorescent complex. Due to the high affinity of Fe 3+ to adenosine-5′-triphosphate (ATP), the fluorescence of the GLY-CDs in GLY-CDs-Fe 3+ could be recovered by ATP. Thereby, quantitatively fluorescent turn-on detection of ATP could be achieved. The fluorescence recovery ratio was linearly proportional to the concentration of ATP with a detection limit as low as 15.0 nM, indicating the CDs have high sensitivity. The GLY-CDs were successfully employed in the detection of ATP in serum and cell lysates.
研究领域:
分析化学
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生物化学
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材料科学
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DOI
PubMed
高影响因子
BIOMATERIALS
·
2016
·
IF 8.387
Gao-Na Shi,Chuang-Nian Zhang,Rong Xu,Jin-Feng Niu,Hui-Juan Song,Xiu-Yuan Zhang,Wei-Wei Wang,Yan-Ming Wang,Chen Li,Xiao-Qing Wei,De-Ling Kong
Whole tumor cell lysates (TCL) have been implemented as tumor antigens for cancer vaccine development, although clinical outcomes of TCL-based antitumor immunotherapy remain unsatisfactory. In order to improve the efficacy of TCL-based vaccines, biomaterials have been employed to enhance antigen delivery and presentation. Here, we have developed chitosan nanoparticles (CTS NPs) with surface mannose (Man) moieties for specific dendritic cells (DCs) targeting (Man-CTS NPs). The Man-CTS NPs were then loaded with TCL generated from B16 melanoma cells (Man-CTS-TCL NPs) for in vitro and in vivo assessment. Potency of the Man-CTS-TCL NPs as cancer vaccine was also assessed in vivo by immunization of mice with Man-CTS-TCL NPs followed by re-challenge with B16 melanoma cell inoculation. We have shown here that Man-CTS-TCL NPs promote bone marrow-derived dendritic cells (BMDCs) maturation and antigen presentation in vitro . In vivo evaluation further demonstrated that the Man-CTS-TCL NPs were readily taken up by endogenous DCs within the draining lymph node (DLN) following subcutaneous administration accompanied by increasing in serum IFN-γ and IL-4 levels. Tumor growth was also significantly delayed in mice primed with Man-CTS-TCL NPs vaccine, attributable at least in part to cytotoxic T lymphocytes response. Moreover, Man-CTS-TCL NPs vaccine also exhibited therapeutic effects in mice with melanoma. Thus, we report here the Man-CTS-TCL NPs as effective anti-tumor vaccine for cancer immunotherapy .
研究领域:
免疫学
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肿瘤学
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生物材料科学
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产品关联
DOI
PubMed
高影响因子
CHEMOMETRICS AND INTELLIGENT LABORATORY SYSTEMS
·
2021
·
IF 3.491
Min-Xi Li,Yao Chen,Zeng-Ping Chen,Ru-Qin Yu
Alkaline phosphatase (ALP) is an important diagnostic indicator of many human diseases. Its detection is of great importance in fields such as clinical diagnosis and biomedical research. In this contribution, a simple label-free fluorescence sensing method for ALP detection was designed by integrating ALP triggered hydrolysis, terminal deoxynucleotidyl transferase assisted DNA polymerization and complex formation between G-quadruplex and thioflavin T together. Furthermore, in order to mitigate the influence of matrix effects and background interference effects on the quantitative results of the proposed fluorescence sensing method encountered in the analysis of real-world complex biological samples, the probe technique-based generalized multivariate standard addition strategy (GMSA probe ) was specifically modified, and combined with the proposed fluorescence sensing method to achieve satisfactory quantitative results for ALP in cell lysate samples with accuracy comparable to that of a commercial ALP assay kit. Its limit of detection and limit of quantification values were about 0.02 and 0.07 U/L, respectively, more than 20 times lower than those of the commercial ALP assay kit. Due to its merits of simplicity, sensitivity, specificity, as well as robustness to matrix effects and background interference effects, the combination of the proposed fluorescence sensing method and the modified GMSA probe has a great potential for ALP assay in biochemical research and clinical diagnosis.
研究领域:
生物化学
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临床诊断
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医学研究
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产品关联
DOI
高影响因子
Stem Cells International
·
2021
·
IF 5.443
Zhipeng Huang,Zhe Zhao,Jun Lang,Wantao Wang,Yinsheng Fu,Wenbo Wang
Super activated platelet lysate (sPL) is a derivative of platelet-rich plasma (PRP) that contains high levels of several growth factors. In this study, we synthesized a temperature-sensitive hydrogel that contained temperature-sensitive Poly(DL-lactide-glycolide-glycolide acid) (PLGA), SrCl2, and HA, and loaded it with different concentrations of sPL. The hydrogel showed satisfactory encapsulation efficiency and release of the growth factors in a sustained manner, indicating its suitability as a drug carrier. The sPL-loaded hydrogel was inserted into the necrotic femoral head of a rat model and core decompression was applied and resulted in significantly accelerated bone repair and regeneration. Therefore, encapsulation of sPL in a hydrogel scaffolding may be an effective strategy for treating femoral head necrosis.
研究领域:
医学
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生物材料学
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骨科
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Junjie Hu,Fei Liu,Nan Feng,Huangxian Ju
The unraveling of enzymatic reactions, especially identification of enzymatic substrates or products, is important to elucidate biological processes. Here a selenium-isotopic signature for mass spectrometric identification of enzymatic-related species is demonstrated by using selenium-containing peptides (SePeps) as substrates. Thus a strategy is proposed for rapid and precise assay of multiple enzyme activity. These SePeps can be synthesized by introduction of one selenomethionine residue in the sequence and simply identified in the full-scan mode with the feature of distinctive selenium-isotopic distribution without MS/MS verifications, which proposes a novel solution to the specific identification of enzyme-related species, allows to exclude the interferences of species with tiny mass differences in bio-samples, and meanwhile can offer a judgement on data accuracy for the analysis of enzyme activities. As a proof-of-concept, a method for multiple analysis of two representative enzymes in MCF-7 cell lysate has been developed with the isotopic peak areas of either SePep substrates or enzymatic products with the top intensities. These results could be the foundation to extend the method for more complicated enzyme systems. The selenium-isotopic signature provides a powerful protocol for high-throughput assays of peptide-metabolizing enzymes with enhanced confidence and can be extended to screen enzymatic reaction-related substrates.
研究领域:
分析化学
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生物化学
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生物技术
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分子生物学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 2
·1-10
·
IF 5.479
Zhang Kaina,Hu Dehua,Deng Suimin,Han Meng,Wang Xiangfeng,Liu Hailing,Liu Yuan,Xie Mengxia
A novel magnetic nanomaterial for use in metal ion based affinity chromatography is described. It is based on the chelation between the phosphate groups of phytic acid (PA) and Ti(IV) ions. Due to the large number (6) of phosphate groups of PA, it has a large capacity for Ti(IV) ions. PA was first immobilized on magnetite nanoparticles (PA-MNPs) and then loaded with Ti(IV) ions to obtain the sorbent (Ti-PA-MNPs). The fraction of Ti(IV) ions on the surface of PA-MNPs that is exposed to the solution binds the phosphate groups of phosphopeptides. The bound phosphopeptides can then be magnetically separated. The method was applied to the enrichment of the phosphopeptides in a β-casein tryptic digest. A tryptic digest of bovine serum albumin (BSA) was added at a molar ratio (β-casein to BSA) of 1:2000 to study selectivity. The phosphopeptides were quantified by mass spectrometry. The limit of detection can be as low as 8 × 10 −10 mol L −1 . This sorbent has a high absorption capacity (53.5 μg mg -1 ) and shows good recoveries (90%). As many as 2145 phosphopeptides were isolated from 500 μg tryptic digest of a rat liver lysate after enrichment by Ti-PA-MNPs. This is superior to that (1568 phosphopeptides) of commercial TiO 2 kit.
Schematic presentation of fabrication for a novel modified magnetic nanomaterial (Ti-PA-MNPs) based on the chelation of phytic acid (PA) with Ti(IV) ions. Ti-PA-MNPs were successfully applied to enriching low abundance phosphopeptides from biosamples in mass spectrometric analysis.
研究领域:
分析化学
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生物化学
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纳米技术
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质谱分析
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CONTROLLED RELEASE
·
2023
·
IF 10.8
Xiangwu Chen,Zeyu Jiang,Yang Lin,Cancan Yu,Xinxin Nie,Guixiang Xu,Wei Xu,Yue Jiang,Yuxia Luan
T cell-based immunotherapy (TCBI) is an emerging approach to combat tumors. However, the outcome of TCBI is still far from satisfaction clinically, owing to stumbling blocks from insufficient immunogenicity , T cell exhaustion and immune evasion from programmed death-1/programmed death-ligand 1 (PD-1/PD-L1) pathway. Herein, an injectable tumor lysates-constructed hydrogel is reported to address these issues. Chemically modified tumor lysates are, for the first time, designed as the gelator to intratumorally construct hydrogel, achieving a robust antigen reservoir to induce strong immunogenicity. Meanwhile, hydrogel-encapsulated nicotinamide riboside and SB415286 enable strong mitophagy in T cells to prevent their exhaustion as well as powerfully genetical suppression of PD-1 expression to regulate immune evasion. Thus, our injectable hydrogel creates a robust immune niche within tumor, enabling to significantly potentiate TCBI. Our strategy pharmacologically regulates body's own T cells in situ, demonstrating potent immunotherapeutic effects and offering a conceptually new approach for TCBI.
研究领域:
肿瘤学
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免疫学
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药学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2021
·
IF 4.759
Kaina Zhang,Yun Hao,Dehua Hu,Suimin Deng,Yuhao Jin,Xiangfeng Wang,Hailing Liu,Yuan Liu,Mengxia Xie
A hydrophilic metal-organic network based on Ti 4+ and dual natural ligand, tannic acid (TA) and phytic acid (PA), has been developed to enrich phosphopeptides from complex bio-samples prior to liquid chromatography-mass spectrometric analysis. Due to the strong chelation ability of TA and PA, abundant Ti 4+ can be immobilized in the material, forming hydrophilic network by one-step coordination-driven self-assembly approach. The sorbent, [email protected] 3 O 4 , exhibited satisfactory selectivity for the phosphopeptides in the tryptic digest of β-casein, and can eliminate the interference components in 1000-fold excess of bovine serum albumin. The adsorption capacity of the sorbents for phosphopeptides was up to 35.2 mg g −1 and the adsorbing equilibrium can be reached in 5 min. The adsorbing mechanism has been investigated and the results indicated that the Ti 4+ in forms of [Ti(f-TA)(H 2 O) 4 ] 2+ , [Ti(f-PA)(H 2 O) 4 ] 2+ and Ti(f-PA) 2 (H 2 O) 2 may play an important role in the adsorption process. The sorbent of the [email protected] 3 O 4 has been applied to enrichment of the phosphopeptides in tryptic digest of rat liver lysate, and 3408 phosphopeptides have been identified, while the numbers of the identified phosphopeptides were 2730 and 1217 when the sample was enriched by the commercial TiO 2 and Fe 3+ -IMAC kit, respectively. This work provides a strategy to enrich phosphopeptides from complex samples and shows great potential application in phosphoproteome research.
研究领域:
分析化学
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生物化学
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蛋白质组学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
You Dou,Ran Yang,Yue Xiao,Jiao Wu,Lingbo Qu,Yuanqiang Sun,Zhaohui Li
The development of light-driven organic nanozymes possessing photoactivity is of great significance in biomedical research and biosensing given that they exhibit prominent absorption and fascinating photo-controllability in the visible range. However, the number and availability of such nanozymes is quite low. In this work, N-arylpyridinium thiazolothiazole (N, N′-dichlorophenzyled dipyridinium thiazolo[5,4- d ]thiazole difluoride, TTz-Cl 2+ ), which exhibits inherent light-driven oxidase-mimicking activity, was synthesized and it was found that 3,3′,5,5′-tetramethylbenzidine (TMB) could be oxidized under visible light, and also underwent corresponding changes in color and absorption. Moreover, the enzymatic activity of TTz-Cl 2+ could be modulated precisely by switching the visible light on and off. Glutathione (GSH), an important biological antioxidation regulator, was shown to be capable of successfully impeding the catalytic functions of TTz-Cl 2+ . Thus, it proved possible to fabricate a facile photo-triggered colorimetric sensing system for detection of GSH based on the use of the TTz-Cl 2+ and TMB system. The sensor exhibited satisfactory analytical performance with a linear range of 1–17 μM and a detection limit of 0.47 μM and may be used to determine GSH in HeLa cell lysates with good selectivity and high sensitivity. This work not only extends the availability of organic nanozymes but also broadens the applications base of thiazolothiazole derivatives in biomedical analysis.
研究领域:
生物医学工程
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分析化学
·
材料科学
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产品关联
DOI
高影响因子
Small
·
2025
·
IF 12.1
Weitao Wang,Haitao Wu,Xuan Zhang,Yang Hong,Shi Tao,Xiangjing Cao,Shipeng Wang,Lisha Zha,Zhengbao Zha
Using entire tumor cells or tissues that display both common and patient-specific antigens can potentially trigger a comprehensive and long-lasting anti-tumor immune response. However, the limited immunogenicity, low uptake efficiency, and susceptibility to degradation of whole-component antigens present significant challenges. In this study, we employed tumor lysates (TLs) as whole-component antigens, in conjunction with MgAl-layered double hydroxide (MA) as nanoadjuvants and Mn 2+ as immunostimulants, to create personalized MMAT (Mn 2+ -MA-TLs) nanovaccines. After subcutaneous injection of MMAT nanovaccines, the high local concentrations of TLs and Mn 2+ facilitated the recruitment and activation of antigen-presenting cells (APCs), thereby inducing a robust adaptive immune response. Remarkably, MMAT nanovaccines enabled lysosomal escape, enhanced antigen cross-presentation, and activated the cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) pathway in APCs. Furthermore, MMAT nanovaccines, when combined with the anti-TIGIT monoclonal antibody (aTIGIT), an immune checkpoint inhibitor, not only stimulated T-cell-based adaptive anti-tumor immune responses but also activated the NK-cell-based innate anti-tumor immunity, effectively suppressing tumor growth, recurrence, and metastasis. Thus, the ternary MMAT nanovaccines developed here introduced a pioneered paradigm for the rapid preparation of whole-component tumor antigens with nanoadjuvants and immunostimulants into nanovaccines, offering new prospects for clinical immunotherapies.
研究领域:
癌症免疫疗法
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免疫学
·
纳米医学
·
肿瘤生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2014
·
IF 2.305
Deependra Tyagi,Javier Batista Perez,Amita Nand,Cheng Zhiqiang,Peizhe Wang,Jie Na,Jingsong Zhu
Surface plasmon resonance imaging (SPRi) has emerged as a versatile biosensor to detect a wide range of biomolecular interactions with divergent potential applications. However, the use of this advanced-level technology for stem cell lysate study is still not much explored. Cell lysates are significant biological analytes used for disease diagnostics and proteomic studies, but their complex nature limits their use as an analyte for SPRi biosensors. Here, we review the problems associated with the use of SPRi for stem cell lysate study and examine the role of surface chemistry, running buffer, and blocking solution in order to minimize nonspecific adsorption (NSA). We detect the expression of Oct4, Sox2, Nanog , Rex1 , and Lin28 biomarkers present in mouse embryonic stem cell (mESC) lysate against their corresponding antibodies immobilized on the sensor surface with reduced NSA. The current study shows that the conjunction of SPRi and microarray can be used as a label-free, high-throughput, and rapid technique for detection of biomarkers and their relative abundance in stem cell lysate study.
研究领域:
生物传感
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干细胞生物学
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蛋白质组学
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分析化学
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生物技术
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产品关联
DOI
PubMed
高影响因子
ACS Biomaterials Science & Engineering
·
2024
·
IF 5.4
Pengcheng Wang,Wenjin Zhong,Qiaozhen Huang,Yuejia Zhu,Liquan Chen,Kai Ye
Treatment with immune checkpoint inhibitors (ICIs) has shown efficacy in some patients with Lynch syndrome-associated colon cancer, but some patients still do not benefit from it. In this study, we adopted a combination strategy of tumor vaccines and ICIs to maximize the benefits of immunotherapy. Here, we obtained tumor-antigen-containing cell lysate (TCL) by lysing MC38Mlh1 KD cells and prepared liposome nanoparticles (Lipo-PEG) with a typical spherical morphology by thin-film hydration. Anti-PD-L1 was coupled to the liposome surface by the amidation reaction. As observed, anti-PD-L1/TCL@Lipo-PEG was not significantly toxic to mouse intestinal epithelial cells (MODE-K) in the safe concentration range and did not cause hemolysis of mouse red blood cells. In addition, anti-PD-L1/TCL@Lipo-PEG reduced immune escape from colon cancer cells (MC38Mlh1 KD) by the anti-PD-L1 antibody, restored the killing function of CD8+ T cells, and targeted more tumor antigens to bone marrow-derived dendritic cells (BMDCs), which also expressed PD-L1, to stimulate BMDC antigen presentation. In syngeneic transplanted Lynch syndrome-associated colon cancer mice, the combination of anti-PD-L1 and TCL provided better cancer suppression than monoimmunotherapy, and the cancer suppression effect of anti-PD-L1/TCL@Lipo-PEG treatment was even better than that of the free drug. Meanwhile anti-PD-L1/TCL@Lipo-PEG enhanced the immunosuppressive tumor microenvironment. In vivo fluorescence imaging and H&E staining showed that the nanomedicine was mainly retained in the tumor site and had no significant toxic side effects on other major organs. The anti-PD-L1/TCL@Lipo-PEG prepared in this study has high efficacy and good biosafety in alleviating the progression of Lynch syndrome-associated colon cancer, and it is expected to be a therapeutic candidate for Lynch syndrome-associated colon cancer.
研究领域:
纳米医学
·
癌症免疫疗法
·
分子生物学
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药学
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）