Foods
·
2022
·Vol. 11
·期 6
·876
·
IF 5.561
Yu Li,Siyuan Fei,Deyang Yu,Lijuan Zhang,Jiaxuan Li,Ronggang Liu,Mingqian Tan
Pickering emulsions stabilized from natural sources are often used to load unstable bio-active ingredients, such as astaxanthin (AXT), to improve their functionality. In this study, AXT-loaded Pickering emulsions were successfully prepared by 2,2,6,6-tetramethy-1-piperidine oxide (TEMPO)-oxidized cellulose nanofibers (TOCNFs) fromUndaria pinnatifida. The morphology analysis showed that TOCNFs had a high aspect ratio and dispersibility, which could effectively prevent the aggregation of oil droplets. The stable emulsion was obtained after exploring the influence of different factors (ultrasonic intensity, TOCNFs concentration, pH, and ionic strength). As expected, AXT-loaded Pickering emulsions showed good stability at 50 °C and 14 days of storage. The results of simulatedin vitrodigestion showed that the emulsions exhibited higher release of free fatty acids (FFAs) and bioaccessibility of AXT than those in sunflower oil. Hence, our work brought new insights into the preparation of Pickering emulsions and their applications in protection and sustained, controlled release of AXT.Keywords:Undaria pinnatifida;nanocellulose;Pickering emulsion;stability;astaxanthin
研究领域:
食品科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF APPLIED PHYCOLOGY
·
2022
·Vol. 34
·期 3
·1709-1719
·
IF 3.404
Li Jing,Pang Shaojun,Su Li,Shan Tifeng
Phlorotannins are among the most important defensive molecules in kelp. They have been majorly identified in brown algae so far. Type III polyketide synthase (PKS) is regarded as one of the enzymes catalyzing a major step in the biosynthetic pathway of phlorotannins. In this study, a type III PKS gene ( sjPKS1 ) is characterized from the economic brown alga Saccharina japonica sporophyte. It contains 7 exons and 6 introns. The sjPKS1 open reading frame consists of 1245 nucleotides encoding a protein of 414 amino acids. The sjPKS1 are 87%, 83%, and 82% identical to that of Ectocarpus siliculosus , Sargassum binderi , and Sargassum fusiforme at the nucleotide level, respectively. While the amino acid sequences of sjPKS1 share more than 90% identity to that of the three brown algae species. The sjPKS1 expression at 200 µmol photons m −2 s −1 was significantly higher than at 20 and 50 µmol photons m −2 s −1 in sporophytic seedlings, so did the content of soluble phlorotannins inside the plants. Seven motifs in response to light are identified in the 5′ flanking region of sjPKS1 and two potential transcription factors are predicted by screening yeast one-hybrid library.
研究领域:
海洋植物学
·
分子生物学
·
遗传学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Qianni Chen,Jiaqi Fan,Lianzhu Lin,Mouming Zhao
The combination of polysaccharides is an effective way to develop prebiotics with stable performance during processing and digestion for human wellness. However, there is little information on optimal screening and complementary regulation of compound polysaccharides. This study aimed to optimally select a combination of Lycium barbarum L. polysaccharide (LBP) and Laminaria japonica polysaccharide (LJP) as a highly efficient prebiotic to regulate the gut probiotics and their metabolites. Two LBPs characterized as rhamnogalacturonan I enriched pectins and two LJPs characterized as fucoidans were obtained by enzyme-assisted acid extraction at moderate and dramatic temperatures and combined in pairs to obtain 4 groups containing 4 proportional combinations. All combinations showed better prebiotic effects than individual LJP. The combination of LBP and LJP extracted at 50 °C at a ratio of 4:1 exhibited the strongest prebiotic effect. The optimal compound polysaccharide achieved superior effect and complementary function via LBP-targeted proliferation of Bifidobacterium , Lactobacillus , and Bacteroides and production of SCFAs and non-SCFA health-associated metabolites, LJP-targeted accumulation of butyrate-producing bacteria and corresponding metabolites, as well as synergistic effect of LJP and LBP at exact proportion. Our study provided theoretical and methodological guidance for optimal screening of compound polysaccharides as new prebiotics.
研究领域:
食品科学
·
微生物学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2024
·Vol. 59
·期 3
·1724-1737
·
IF 2.6
Yihan Sun,Jiaying Ren,Xiang Li,Kailing Sun,Dingding Feng,Xiuping Dong,Hang Qi
Summary Rich in numerous nutrients, undaria pinnatifida ( UP ) exhibited a broad spectrum of biological properties, including anti-inflammatory, anti-bacterial, anti-cancer, and antioxidant properties. A sexual pigment protein complex known as phycocyanin (PC) was the perfect photosensitiser and had no negative effects. Due to their superior qualities, hydrogels were also widely employed in tissue engineering, medication administration, and food packaging at the same time. The aim of this work was to investigate the impact of UVB irradiation on the gel characteristics of PC- UP hydrogels using UVB irradiation technology rather than chemical crosslinking approach. The results showed that the G ′ value of PC- UP hydrogel increased from 114.17 ± 0.58 Pa to 156.72 ± 0.82 Pa and the G ″ value increased from 36.80 ± 0.79 Pa to 58.05 ± 1.06 Pa after UVB irradiation. At the same time, the texture properties of hydrogel were significantly improved, and the hardness, adhesion and chewiness were increased to 214.02 ± 3.85, 198.51 ± 4.25 and 195.81 ± 5.69, respectively. After UVB irradiation, hydrogen bond was formed in PC- UP hydrogel to improve its thermal stability. In conclusion, the results show that PC decomposes with alginic acid, potassium salt, and sodium salt in UP under UVB irradiation to form a gel network structure, which enhances its gel properties. This study provides guidance for the photo response of PC- UP hydrogels, provides ideas for the application of PC- UP hydrogels, and broadens the application field of future hydrogels.
研究领域:
食品科学
·
生物材料学
·
海洋生物学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Functional Foods
·
2025
·
IF 4
Biao Yu,Baojun Xu,Karsoon Tan,Saiyi Zhong,Kit-Leong Cheong
Undaria pinnatifida oligosaccharides (UPO) are recognized for their multifaceted bioactive properties. Nevertheless, further investigation is required to establish the protective effects of UPO in mitigating UC. In this study, the structural characteristics of UPO were elucidated as 1,4-linked α-L-fucopyranose units with minor 1,2-linked β- d -galactopyranose units through NMR spectroscopy. UPO treatment improved the structural integrity of the intestinal barrier in DSS-induced colitis mice. Additionally, UPO administration induced the polarization of macrophages towards the anti-inflammatory M2 phenotype, evidenced by the downregulation of M1 macrophage marker (CD68) and upregulation of M2 macrophage marker (CD163). UPO treatment, significant alterations in the composition and diversity of bacterial and fungal microbiota were observed compared to colitis mice. UPO boosted the concentrations of short-chain fatty acids in fecal samples. These findings indicate that UPO could alleviate DSS-induced colitis in mice, potentially offering insights into its application as a novel therapeutic option for colitis treatment.
研究领域:
食品科学
·
免疫学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Zhao-Rong Li,Rui-Bo Jia,Xueyuan Cai,Donghui Luo,Chong Chen,Mouming Zhao
The current study was aimed to enhance the solubility, dispersibility and biotransformation efficacy of ellagic acid (EA) by preparing food-derived ellagic acid- Undaria pinnatifida polysaccharides solid dispersion (EA/UPP SD). The results demonstrated that the solubility of EA/UPP SD was improved from 0.014 mg/mL to 0.383 mg/mL, and the enhancement was related to converting to a more amorphous state and restraining its self-aggregation during the mechanochemical process. The structure of EA/UPP SDs was mostly maintained by hydrogen bonds and hydrophobic interactions between EA and UPP . Moreover, the result of in vitro anaerobic incubations showed the biotransformation process was improved with EA/UPP SD addition to substrate due to the advance of microbial accessibility in EA dispersion. Altogether, these results indicated that the EA/UPP SDs expanded the application of EA by increasing the solubility and dispersity, and provided a theoretical basis for bioconversion efficiency enhancement.
研究领域:
食品科学
·
药学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Oceanology and Limnology
·
2022
·Vol. 41
·期 1
·300-313
·
IF 1.554
Ke Songze,Zhang Bo,Yu Yanlei,Wang Sijia,Jin Weihua,Wu Jian,Chen Jianwei,Zhang Huawei,Wei Bin,Wang Hong
A sulfated galactofucan designated as UpG was obtained from the brown algae Undaria pinnatifida by calcium chloride extraction. Chemical analyses showed that UpG is composed of galactose and fucose at a high sulfation level. Low-molecular weight UpGP-0.5 was prepared from UpG through acid hydrolysis for structure characterization. The backbones of UpG are determined to be α-(1,3)-Fuc, α-(1,4)-Gal, α-(1,3)-Gal, and α-(1,6)-Gal by GC-MS, FT-IR, NMR, and LC-MS analyses. Sulfate groups are modified at C2 and/or C4 of fucose and C3 and/or C4 of galactose. UpG could partially lower blood sugar and serum lipid levels in type 2 diabetic mice. Moreover, UpG treatment regulates the abundance of some specific gut microbiota, such as enriching the abundance of Muribaculum and Christensenellaceae, and reducing that of Bilophila , Tannerellaceae, Candidatus Saccharimonas and Anaerotruncus . The findings characterized the detailed structure of a sulfated galactofucan and investigated its potential for the treatment of type 2 diabetes mellitus.
研究领域:
海洋生物学
·
药理学
·
营养学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Ping Shao,Haoya Zhang,Ben Niu,Ligang Jiang
It was previously found that R-(+)-Limonene emulsion could be stabilized by Ulva fasciata polysaccharide . In the present study, emulsions of R-(+)-Limonene were developed for coating of strawberries to improve the shelf life and microbiological safety. Edible coatings with at least 0.15% w/w of R-(+)-Limonene improved the microbial stability of the strawberries, resulted effective in the decontamination of external pathogens such as Escherichia coli , Bacillus subtilis and Staphylococcus aureus . Changes in weight loss, total soluble solid (TSS), pH, total phenolic, ascorbic acid , textural properties and surface color of strawberries during storage were evaluated. R-(+)-Limonene emulsions were effective for decreasing water loss of all the fruit samples tested. The decrease in TSS values was higher in the control strawberries. Initial decreases followed by increases of pH during storage of strawberries. The amount of total phenols detected in control fruit sharply increased during the first 6 days then remained steady, whereas the total soluble phenols content of treated strawberries gradually increased during storage. Strawberries coated with emulsion had higher L* and a* values as compared to control fruits. The R-(+)-Limonene coatings have demonstrated the potential to inhibit foodborne pathogen contamination of strawberries, and prolong their shelf life.
研究领域:
食品科学
·
植物化学
·
微生物学
✓ 已验证
DOI
高影响因子
Antioxidants
·
2023
·Vol. 12
·期 5
·1015
·
IF 7
Xue-Wu Chen,Yu-Yun Zheng,Jian-Ming Ouyang
The clinical manifestation of primary hyperoxaluria includes hyperoxaluria and recurrent urinary calculi. In this study, an oxidative damage model was constructed based on oxalate damage to the human renal proximal tubular epithelial cells (HK-2), and a comparative study was carried out on four different sulfated levels ofUndaria pinnatifidapolysaccharides (UPP0, UPP1, UPP2, and UPP3 with sulfate group [–OSO3−] contents of 1.59%, 6.03%, 20.83%, and 36.39%, respectively) on the repair of oxidatively damaged HK-2 cells. The results showed that after repair by UPPs, cell viability was enhanced, healing ability was improved, the intracellular superoxide dismutase level and mitochondrial membrane potential were increased, malondialdehyde, reactive oxygen species, and intracellular Ca2+levels were reduced, cellular autophagy was reduced; lysosomal integrity was improved, and cytoskeleton and cell morphology were restored. The ability of repaired cells to endocytose nano-calcium oxalate dihydrate crystals (nano−COD) was enhanced. The activity of UPPs was closely related to their –OSO3−content. A too high or too low –OSO3−content was not conducive to polysaccharide activity, and only UPP2 exhibited the best cell repair ability and strongest ability to promote the cell endocytosis of crystals. UPP2 may be used as a potential agent to inhibit CaOx crystal deposition caused by high oxalate concentration.Keywords:Undaria pinnatifidapolysaccharide;acidic groups;cell repair;crystal endocytosis;primary hyperoxaluria
研究领域:
肾病学
·
药理学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food & Function
·
2022
·Vol. 13
·期 5
·2581-2593
·
IF 6.317
Panpan Zhang,Jinhui Jia,Pingrui Jiang,Weiyun Zheng,Xiangfei Li,Shuang Song,Chunqing Ai
Brown seaweed is rich in polysaccharides including sulfated polysaccharides and alginate, both of which provide health benefits to the host but whose differences have not received sufficient attention. In this study, alginate from Undaria pinnatifida (UPA) and sulfated polysaccharides from U. pinnatifida (UPSP) were isolated, and their action was analyzed in high fat diet-fed mice. UPA and UPSP improved body composition, fat deposition in body tissues and organs, lipid abnormality and inflammatory response in mice, and compound polysaccharides from U. pinnatifida (UPP: UPA + UPSP) had a better effect on some physiological indexes, which could be attributed to the differences in the gut microbiota. Both UPSP and UPA modulated diet-induced microbiota dysbiosis, and UPP had better effect on changes in the gut microbiota, including an increase in Bacteroidales and reduction in both Clostridiales and Lactobacillales that had positive correlations with the improvement of the physiological status. The in vitro model of bacterial culture revealed that the use of Bacteroides on UPA and UPSP was species dependent, and UPP can better maintain the diversity and stability of the Bacteroidales community. This study indicated that polysaccharides from edible brown seaweed can benefit host health by improving the intestinal microecology, which can be helpful for the application of edible brown seaweed in health foods.
研究领域:
营养学
·
微生物学
·
食品科学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Chun-yan Peng,Quan-yuan Xie,Xing Xie,Lin-yi Tang,Tian-xin Ma,Dai-wei Ke,Zong-cai Tu,Lu Zhang
Undaria pinnatifida (Up) is an edible seaweed known for its abundant nutrients and active compounds. In this research, six different methods, including acid hydrolysis extraction (AHE), alkaline hydrolysis extraction (LHE), enzymatic hydrolysis extraction (EHE), as well as their combinations with ultrasonic assisted extraction (AHE-U, LHE-U, EHE-U), were applied to extract non-extractable polyphenols from Up (UNEPPs). Results revealed that LHE-U was the most effective way to extract UNEPPs, it gave the highest yield (1.26 %) and total phenolics content (29.88 μg GAE/mg E), as well as considerable antioxidant and in vitro hypoglycemic effects. HPLC-Q-TOF-MS/MS analysis revealed the identification of 36 compounds from ULNEPPs, ferulic acid, p-coumaric acid and ferulic acid 4-O-glucuronide were the major compounds. In vivo study found that ULNEPPs could reduce fasting blood glucose (FBG) level, ameliorate abnormal glucose metabolism and dyslipidemia, repair insulin resistance and pancreas islet damage in type 2 diabetes (T2D) mice. Additionally, RT-qPCR analysis revealed that ULNEPPs improved glucose metabolism through the up-regulation of gene expression levels of Pi3k , Glut4 , Akt , Ampk and the down-regulation of gene expression levels of Foxo1 , Pgc-1α , Gsk-3β , Glut4 , and G6pc . These results evidence that has the potential as dietary ingredients for preventing and treating T2D.
研究领域:
药理学
·
营养学
·
生物化学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Pingrui Jiang,Weiyun Zheng,Xiaona Sun,Guoping Jiang,Shuang Wu,Yuxin Xu,Shuang Song,Chunqing Ai
Undaria pinnatifida was shown to reduce serum lipids and fat accumulation and produce beneficial effect on type 2 diabetes, but its effect on intestinal micro-ecology remains unclear. This study showed that sulfated polysaccharides from U. pinnatifida (UPSP) reduced weight gain, fat accumulation and metabolic disorders in mice fed with high fat diet (HFD). UPSP not only alleviated HFD-induced microbiota dysbiosis indicated as increased abundances of some Bacteroidales members that had positive correlations with the improvement of physiological indexes, but also maintained gut barrier integrity and reduced metabolic endotoxemia. A dose-effect relationship was observed between the dose of UPSP and its effect on some physiological indexes, gut microbiota community and nutrient utilization. The in vitro result showed that the use of Bacteroides species within Bacteroidales on UPSP was species-dependent, and the dose of UPSP affected the growth properties of some Bacteroides species. It implied that UPSP can be considered as prebiotic agent to prevent gut dysbiosis and obesity-related diseases in obese individuals.
研究领域:
营养学
·
微生物学
·
药理学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）