FOOD CHEMISTRY
·
2021
·
IF 7.514
Zheng-Hao Yin,Yuan-Fei Li,Hong-Xia Gan,Nan Feng,Yong-Ping Han,Li-Mei Li
Sanggenone C, oxyresveratrol, catechin and l -epicatechin exist in Morus and Hulless Barley as natural polyphenols with antityrosinase activity. Little research on their synergistic and structure–function relationships of them has been reported in recent years. In this paper, the inhibition mechanisms of these four plant polyphenols were investigated by enzyme kinetics, HPLC, fluorescence spectra, and molecular docking methods. The results showed that oxyresveratrol (IC 50 = 1.096 ± 0.048 μg/mL), sanggenone C (IC 50 = 13.360 ± 1.029 μg/mL), l -epicatechin (IC 50 = 55.730 ± 1.762 μg/mL), and catechin (IC 50 = 148.500 ± 3.355 μg/mL) exhibited tyrosinase inhibition activity. When sangenone C (14 μg/mL) was mixed with l -epicatechin (56 μg/mL) at 4:1 (40 μL + 10 μL), the highest tyrosinase inhibition was achieved. Molecular docking showed that the number and position of phenolic hydroxyls of polyphenols were the key for tyrosinase inhibition activity. This study provided new ideas for the application of these four plant polyphenols from Hulless Barley and Morus as tyrosinase inhibitors in food preservation.
研究领域:
食品科学
·
植物化学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2019
·
IF 3.714
Qing Li,Shihua Yang,Yongqiang Li,Yonghua Huang,Jianping Zhang
Phenolic compounds associated with dietary fibre are present in free, soluble conjugated and insoluble-bound forms. In this study, total soluble, free, esterified, etherified and insoluble-bound phenolic fractions were extracted from the insoluble dietary fibre (IDF) and soluble dietary fibre (SDF) of hulless barley. Total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activities of five phenolic fractions of IDF and SDF were evaluated. Major phenolic acids and flavonoids were analyzed by high performance liquid chromatography. The results revealed that the TPC and TFC of five phenolic fractions of IDF were 2.42–7.33 and 3.08–10.00 times, respectively, which were higher than those in SDF. Insoluble-bound phenolic fractions had the highest TPC and TFC among the five phenolic fractions. A significant positive linear correlation between TPC and antioxidant activity was observed in each of the five phenolic fractions from IDF and SDF ( P < 0.05). Furthermore, ferulic acid was an abundant phenolic compound in total soluble and insoluble-bound phenolic fractions among IDF and SDF. This study indicates that IDF and SDF are rich in phenolic compounds which can be considered as functional food ingredients or nutraceuticals.
研究领域:
食品科学
·
营养学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
STARCH-STARKE
·
2023
·
IF 2.3
Yijuan Xu,Weifeng Hua,Haoran Xu,Harold Corke,Wuyang Huang,Zhongquan Sui
Hulless barley (HB) grass is a potential resource of functional food. In this study, the proliferation inhibitory effect against cancer cells of an alkaline extracted polysaccharide (APP) from HB grass (HBG) is analyzed. The yield of crude alkaline extracted polysaccharide (APC) reaches 15.1%. After purification, the purity and polydispersity index (PDI) of the APP are 98.3% and 1.38, respectively. The molecular weight of APP is 5.1 × 10 5 Da, whereas the number-average molecular weight of APP is 3.7 × 10 5 Da. APP is composed of arabinose (37.8%), xylose (41.3%), glucose (4.6%), and galactose (16.3%). According to the infrared spectral analysis, APP is found to contain β-glycosidic bonds. Moreover, APP possesses significant cell proliferation inhibitory effects against various cancer cell lines, such as 4T1, HT29, Caco-2, and CT26.WT in a dose-dependent manner. Notably, the IC50 value of 4T1 reaches 7.56 mg mL −1 , which indicates that APP from HBG has a potential anti-cancer effect in vitro. It may be further investigated as an ingredient or nutraceutical for development and utilization in functional foods to prevent or treat cancer and related diseases.
研究领域:
食品科学
·
癌症研究
·
药理学
·
植物化学
✓ 已验证
产品关联
DOI
高影响因子
ACS Omega
·
2020
·
IF 2.87
Bianling Jiang,Fuyun Geng,Ran Chang,Mengting Ruan,Ying Bian,Lisheng Xu,Fan Feng,Qiong Chen
The present study was executed to analyze the functional phytochemicals of hulless barley grass grown under different intensities of ultraviolet stress. The wheat seedlings were imposed to 0.5, 1.0, 1.5, 2.0, and 2.5 h ultraviolet radiation and harvested in different times at vegetative stage. Specifically, the contents of total polyphenols, total flavonoids, total triterpenes, total polysaccharides, proanthocyanidins, and chlorophyll were determined and antioxidants capacity was evaluated by OH• and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) scavenging ability. A mathematical model (Technique for Order Preference by Similarity to Ideal Solution, TOPSIS) was also employed for the comprehensive evaluation of functional components of hulless barley grass at different growth stages. The results showed that the UV stress could efficiently improve/preserve the contents of total polyphenols, total flavonoids, total triterpenes, total polysaccharides, proanthocyanidins, chlorophyll a, chlorophyll b, and total chlorophyll, as well as the OH• and ABTS scavenging capacity. TOPSIS evaluation revealed that the highest phytochemical contents were yield on the 15th day under 1.0 h ultraviolet treatment.
研究领域:
植物生理学
·
植物化学
·
农学
·
环境胁迫生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）