Scientific Reports
·
2025
·Vol. 15
·期 1
·1-14
·
IF 3.9
Yue Qiulin,Liu Yongxuan,Li Fujia,Hong Tao,Guo Shousen,Cai Mengrui,Zhao Lin,Su Le,Zhang Song,Zhao Chen,Li Kunlun
Fucoidan is a fucose-rich sulfated polysaccharide that has gained attention owing to its various biological activities. In this study, fucoidan was isolated from Saccharina japonica using an enzyme-assisted method, and its antioxidant and anti-hepatocarcinoma effects were evaluated. The fucoidan was a 112.8 kDa polysaccharide comprising seven monosaccharides: fucose, xylose, glucuronic acid, rhamnose, glucose, mannose, and galactose. The main chain residues were (1 → 3)-α-L-Fuc p and (1 → 4)-α-L-Fuc p units with sulfate groups at the C-2/C-4 positions of the (1 → 3)-α-L-Fuc p residues. S. japonica fucoidans showed excellent antioxidant potency with values of 1.02 mg TE/g and 5.39 mg TE/g for the ABTS and FRAP assays, respectively. Additionally, they exerted antitumor efficacy and low systemic toxicity in H22 tumor-bearing mice, with a tumor inhibition rate of 42.93%. Furthermore, it significantly inhibited tumor angiogenesis and reduced pro-inflammatory cytokines levels (IL-1β, IL-6, and TNF-α). Our results suggest that fucoidan isolated from S. japonica possesses potent antioxidant and anticancer properties and may be used as a potential agent for hepatocellular carcinoma treatment.
研究领域:
海洋生物学
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药理学
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOTECHNOLOGY LETTERS
·
2019
·Vol. 41
·期 10
·1187-1200
·
IF 2.154
Huang Guiyuan,Wen Shunhua,Liao Siming,Wang Qiaozhen,Pan Shihan,Zhang Rongcan,Lei Fu,Liao Wei,Feng Jie,Huang Shushi
Objectives Bifunctional alginate lyase can efficiently saccharify alginate biomass and prepare functional oligosaccharides of alginate. Results A new BP-2 strain that produces alginate lyase was screened and identified from rotted Sargassum . A new alginate lyase, Alg17B, belonging to the polysaccharide lyase family 17, was isolated and purified from BP-2 fermentation broth by freeze-drying, dialysis, and ion exchange chromatography. The enzymatic properties of the purified lyase were investigated. The molecular weight of Alg17B was approximately 77 kDa, its optimum reaction temperature was 40–45 °C, and its optimum reaction pH was 7.5–8.0. The enzyme was relatively stable at pH 7.0–8.0, with a temperature range of 25–35 °C, and the specific activity of the purified enzyme reached 4036 U/mg. A low Na + concentration stimulated Alg17B enzyme activity, but Ca 2+ , Zn 2+ , and other metal ions inhibited it. Substrate specificity analysis, thin-layer chromatography, and mass spectrometry showed that Alg17B is an alginate lyase that catalyses the hydrolysis of sodium alginate, polymannuronic acid (polyM) and polyguluronic acid to produce monosaccharides and low molecular weight oligosaccharides. Alg17B is also bifunctional, exhibiting both endolytic and exolytic activities toward alginate, and has a wide substrate utilization range with a preference for polyM. Conclusions Alg17B can be used to saccharify the main carbohydrate, alginate, in the ethanolic production of brown algae fuel as well as in preparing and researching oligosaccharides.
研究领域:
海洋生物学
·
酶学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2022
·
IF 11.889
Weiming Li,Lihui Lu,Chi Cheng,Nanqi Ren,Shang-Tian Yang,Meng Liu
In this study, mannitol and mannitol-rich seaweed were fermented to investigate the relationship between substrate reduction degree and hydrogen production performance. The results showed that acetate was required in mannitol fermentation with an optimum acetate/mannitol mass ratio of 1:5. Hydrogen production and yield of mannitol fermentation reached 123.76 mL and 2.12 mol/mol-mannitol, respectively, 42.02 % and 26.95 % higher than that of glucose, respectively. The acetate was fully assimilated and the butyrate selectivity reached 100 % in the effluent. Redox potential and electron distribution showed that mannitol increased the overall electron input from mannitol and acetate, leading to the increase in hydrogen and butyrate generation. Hydrogen yield reached 2.33 mol/mol-mannitol with brown algae hydrolysate, which was the highest ever reported. This study demonstrated that substrate with a higher reduction degree could yield higher hydrogen and showed the great application potential of brown algae fermentation for the co-production of hydrogen and butyrate.
研究领域:
微生物学
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可再生能源
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Jiaying Ren,Yujing Lu,Yu Liu,Xu Huang,Kailing Sun,Hang Qi
Carotenoids, recognized for their antioxidant and anti-aging properties, are commonly used in functional foods. To enhance the application of fucoxanthin (FX) in the food industry, this study employed the ion gel method for encapsulating FX and combined it with raw materials such as Undaria pinnatifida homogenate and apple pieces to create freeze-dried crunchy chunks. The study evaluated the effects of encapsulated-FX on the functional and structural characteristics of the Undaria pinnatifida and apple freeze-dried chunks over accelerated storage period under high temperature and humidity. Various analyses were conducted, including physicochemical properties, texture analysis, color evaluation, sensory assessment, and simulated digestion analysis. The results demonstrated that the FX-rich freeze-dried crunchy chunks exhibited favorable structural properties and appealing flavor. Notably, after the accelerated storage period, the encapsulated-FX maintained significant antioxidant activity, along with excellent thermal and light stability, indicating high storage stability. Additionally, the main ingredients, sodium alginate (SAA) and pectin (PE), significantly enhanced the stability of FX during in vitro digestion. This study provided a straightforward approach for producing freeze-dried snack foods rich in stabled-FX, contributing to the diversity of value-added algae products. Furthermore, it laid a theoretical foundation and reference for the future development of nutritional products.
研究领域:
食品科学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Qianni Chen,Lianzhu Lin,Mouming Zhao
Fucoidan possesses a variety of biological activities beneficial for human wellness. It is a good candidate for ameliorating obesity and diabetes. However, high-purity fucoidan products are limited due to the co-extraction of impurities i.e. alginate, protein and small molecular substances. In this study, the optimal preparation strategy for high-purity fucoidan from a template brown alga ( Saccharina japonica ) was screened out by the exploration of key and auxiliary steps. The key step was confirmed as moderate-temperature acid extraction and the auxiliary steps were fixed on ethanol refluxing and ball milling pretreatment. The fucose content of S. japonica fucoidan increased by 7.15 times and the sulfate group content increased by 4.87 times compared with those of fucoidans prepared by classic high-temperature acid extraction. Ball milling-assisted moderate-temperature acid extraction performed great effectiveness and universality in the extraction of high-purity fucoidans derived from four brown algae including S. japonica , Undaria pinnatifida, Sargassum fusiforme, and Fucus vesiculosus . Glycolipid-lowering activities of four fucoidans were confirmed by their interactions with intestinal bile acid, cholesterol micellar, glucose and probiotics including butyrate-producing bacteria, Bacteroides , Bifidobacterium , and Lactobacillus . Our study provided an efficient and universal extraction process for high-purity fucoidan with glycolipid-lowering activity from typical brown algae.
研究领域:
海洋生物学
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食品科学
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生物化学
✓ 已验证
产品关联
DOI
高影响因子
Food Bioscience
·
2022
·
IF 5.318
Fen Yan,Jinfu Zhong,Junying Chen,Wenjing Liu,Xiaojie Chen
Alginate oligosaccharide (AOS) was enzymatically prepared and had a degree of polymerization (DP) of 2–6. AOS exhibited evident antioxidant efficacy in ABTS + scavenging activity, hydroxyl radical scavenging capacity, and total reducing power. The freshness of the prawns, which were treated with AOS and ε-polylysine (ε-PL), was evaluated by measuring indicators such as total viable bacteria (TVC), pH, total volatile basic nitrogen (TVB-N), sensory, texture, and melanosis during storage period. The texture properties and sensory scores of prawns treated with AOS and ε-PL were significantly improved ( P < 0.05) in comparison with the control group. Meanwhile, the increase in TVC, pH, TVB-N, and melanosis scores of prawns treated with AOS and ε-PL were effectively delayed ( P < 0.05) in comparison with control group. Moreover, the quality of prawns treated with 8 g/L AOS was better than that of prawns treated with 6 g/L. AOS could prolong the shelf life of prawns by 2 days at least. Therefore, AOS is a natural biological preservative in providing an effective and safe way to preserve freshness of prawns.
研究领域:
食品科学
·
海洋生物学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2016
·
IF 4.052
Mei-Jia Shi,Xiaoyi Wei,Jie Xu,Bing-Jie Chen,De-Yin Zhao,Shuai Cui,Tao Zhou
In order to improve the bioactivities of the polysaccharide from Enteromorpha prolifera (PE), crude PE (Mw 1400 kDa) was degraded to low molecular weight polysaccharide (44 kDa) in the presence of hydrogen peroxide/ascorbic acid, followed by carboxymethylation. The reaction conditions for carboxymethylation of degraded polysaccharide (DPE) were optimized by Response Surface Methodology. The carboxymethyled degraded polysaccharide (CDPE) obtained under optimized conditions, with a degree of carboxymethylation of 0.849, was characterized by FT-IR and 13 C NMR. The molecular weight of CDPE was measured to be 53.7 kDa. CDPE was evaluated for its antioxidant activity by determining the ability to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl and superoxide anion radicals, and by determining the ferric reducing power. The antioxidant activity of CDPE was found to be greatly improved in comparison with degraded polysaccharide (DPE) and crude polysaccharide from Enteromorpha prolifera (PE).
研究领域:
生物化学
·
食品科学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
·
2024
·
IF 5.9
Hailan Wang,Fu Gu,Xiulian Ren,Qifeng Wei
The utilization of algal ingredients for producing functional products has garnered significant attention. Furthermore, increasing the yield of polysaccharides has become a crucial point. In this work, the efficiencies of two-step extraction and co-extraction were compared, and dimethylamine solution was selected as the optimal extractant. Under the optimum extraction conditions (temperature of 75.00 °C, time of 3.15 h, liquid-solid ratio of 35.00 mL/g) determined by response surface methodology, the yield of alginate was 16.05 ± 0.37 % and the yield of fucoidan was 2.85 ± 0.08 %. Kinetic analysis of the extraction process was conducted using the shrinking core model, elucidating the rate and mechanism of product release. Additionally, it was demonstrated that the dimethylamine solution could be recycled for five times with stable extraction performance. The extraction mechanism was further analyzed by quantum chemical calculations, providing theoretical support for the extraction process of the products. The above results demonstrated that the higher yield of products extracted with dimethylamine was confirmed through experimental and quantum chemical analysis. Overall, this study provides a new perspective on the extraction of alginate and fucoidan from seaweed.
研究领域:
海洋生物学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
PLoS One
·
2014
·Vol. 9
·期 4
·e95242
·
IF 3.534
Hui-Xi Zou,Nan Li,Li-Hua Wang,Ping Yu,Xiu-Feng Yan
A fundamental investigation of the biosorption of Cd 2+ from aqueous solution by the edible seaweed Sargassum fusiforme was performed under batch conditions. The influences of experimental parameters, such as the initial pH, sorption time, temperature, and initial Cd 2+ concentration, on Cd 2+ uptake by S. fusiforme were evaluated. The results indicated that the biosorption of Cd 2+ depended on the initial Cd 2+ concentration, as well as the pH. The uptake of Cd 2+ could be described by the Langmuir isotherm model, and both the Langmuir biosorption equilibrium constant and the maximum biosorption capacity of the monolayer decreased with increasing temperature, thereby confirming the exothermic character of the sorption process. The biosorption kinetics follows the pseudo-second-order kinetic model, and intraparticle diffusion is the sole rate-limiting step for the entire biosorption period. These fundamental equilibrium and kinetic results can support further studies to the removal of cadmium from S. fusiforme harvested from cadmium-polluted waters.
研究领域:
环境科学
·
海洋生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SOLAR ENERGY MATERIALS AND SOLAR CELLS
·
2018
·
IF 5.018
Mingjun Pan,Shan Zhao,Long Ma,Nan Wu,Debao Xiao
Two novel all-in-one electrochromic devices have been fabricated on the basis of low-cost and environmentally benign marine brown algae laminaria japonica, and jellyfish, which were both utilized as electronic component (gel electrolytes) in combination with electrochromic viologen bis(3-hydroxypropyl) viologen dibromide, and electron mediators 1,1′-ferrocene dicarboxylic acid and 1,1′-ferrocenedimethanol. The electrochromic performance of the as-fabricated devices was tested. The two biological ECDs exhibited driving voltages as low as 1.1 V, which is superior to many traditional viologen-based ECDs. Moreover, following the principles of green chemistry, no waste and organic solvents were introduced during the room-temperature device assembly. Based on abundant content of biological tissues, the device can be presented as a proof-of-concept to find potential applications in the fields of low-cost, green and large-scale ECDs.
研究领域:
材料科学
·
生物学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2023
·Vol. 12
·期 16
·3000
·
IF 5.2
Jialiang Chen,Zheng Zhou,Ping Li,Shuhong Ye,Wei Li,Ming Li,Lin Zhu,Yan Ding
Type 2 diabetes mellitus (T2DM) has developed into an important health concern worldwide. The discovery of phlorotannins and their efficacy in the treatment of T2DM has become a hotspot for research in various fields. In this study, the potential phlorotannins and mechanism of six brown algae against T2DM were in-depth investigated using biological activity assays, LC-MS, and network pharmacology. First, the ethyl acetate fraction (EA frac.) showed high polyphenolic content and possessed significantly antioxidant and enzyme inhibitory abilities. Further, a total of fifty-nine peaks were obtained from six EA fracs. via UPLC-QE-MS/MS analysis, and fifteen of them were identified as phlorotannins and their isomers or derivatives. In detail, the chemical structures of six phlorotannins were inferred as dibenzodioxine-1,3,6,8-tetraol, bifuhalol, dioxinodehydroeckol, eckol, fucofurodiphlorethol, and fucotriphlorethol; three phlorotannin isomers were deduced to be fucophlorethol, trifucol, triphlorethol A, or triphlorethol B; and the phlorotannin derivative ofm/z263 was determined to be dibenzodioxine-1,2,3,6,8-pentanol or dibenzodioxine-1,2,4,5,7-pentanol. Moreover, 43 T2DM-related targets acted on by these chemicals were identified, and the function of phlorotannin to prevent and treat T2DM was elucidated in a holistic way based on the established compound-target-disease network, and GO function and KEGG pathway enrichment analysis.Keywords:phlorotannins;T2DM;UPLC-QE-MS/MS;network pharmacology
研究领域:
药理学
·
生物化学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE
·
2024
·Vol. 90
·期 1
·e17647
·
IF 3.2
Jingyu Gao,Fan Wu,Mingzhu Yan,Xuejiang Wang,Yan Chi,Yu Zhang,Yu Peng,Mo Li,Yuanying Ni,Xin Wen
Brown seaweed oligosaccharides (BSO) have demonstrated potential as nutraceuticals with cholesterol-lowering, anti-obesity, and anti-constipation properties. In this study, we initially examined how BSO impact body weight, blood lipid levels, and adipose tissue in a rat model of obesity induced by a high-fat diet. Our findings revealed that BSO administration significantly attenuated body weight gain, ameliorated dyslipidemia, and reduced visceral adiposity. Additionally, we evaluated the prophylactic efficacy of BSO in a loperamide hydrochloride-induced constipation mouse model by assessing defecation parameters and gastrointestinal motility. Our results indicated that BSO intervention significantly increased fecal pallet output and fecal water content, as well as improved intestinal transit. In conclusion, this study lays the foundation for a deeper understanding of how BSO may influence obesity and constipation, offering novel insights into its potential safe and efficacious clinical applications.
研究领域:
营养学
·
代谢生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）