Cells
·
2023
·Vol. 12
·期 5
·771
·
IF 6
Kui Kang,Mengyi Zhang,Lei Yue,Weiwen Chen,Yangshuo Dai,Kai Lin,Kai Liu,Jun Lv,Zhanwen Guan,Shi Xiao,Wenqing Zhang
Plants produce diverse secondary compounds as natural protection against microbial and insect attack. Most of these compounds, including bitters and acids, are sensed by insect gustatory receptors (Grs). Although some organic acids are attractive at low or moderate levels, most acidic compounds are potentially toxic to insects and repress food consumption at high concentrations. At present, the majority of the reported sour receptors function in appetitive behaviors rather than aversive taste responses. Here, using two different heterologous expression systems, the insectSf9cell line and the mammalian HEK293T cell line, we started from crude extracts of rice (Oryza sativa) and successfully identified oxalic acid (OA) as a ligand ofNlGr23a, a Gr in the brown planthopperNilaparvata lugensthat feeds solely on rice. The antifeedant effect of OA on the brown planthopper was dose dependent, andNlGr23amediated the repulsive responses to OA in both rice plants and artificial diets. To our knowledge, OA is the first identified ligand of Grs starting from plant crude extracts. These findings on rice–planthopper interactions will be of broad interest for pest control in agriculture and also for better understanding of how insects select host plants.Keywords:ligand identification;gustatory receptor;Nilaparvata lugens;oxalic acid;antifeedant
研究领域:
昆虫学
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植物科学
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农业科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Jie Chen,Qin Liu,Longyu Yuan,Wenzhong Shen,Qingxing Shi,Guojun Qi,Ting Chen,Zhenfei Zhang
The brown planthopper (BPH) is the most serious pest causing yield losses in rice. MicroRNAs (miRNAs) are emerging as key modulators of plant–pest interactions. In the study, we found that osa-miR162a is induced in response to BPH attack in the seedling stage and tunes rice resistance to the BPH via the α-linolenic acid metabolism pathway as indicated by gas chromatography/liquid chromatography-mass spectrometry analysis. Overexpression of osa-miR162a inhibited the development and growth of the BPH and simultaneously reduced the release of 3-hexenal and 3-hexen-1-ol to block host recognition in the BPH. Moreover, knockdown of OsDCL1, which is targeted by osa-miR162a, inhibited α-linolenic acid metabolism to enhance the resistance to the BPH, which was similar to that in miR162a-overexpressing plants. Our study revealed a novel defense mechanism mediated by plant miRNAs developed during the long-term evolution of plant–host interaction, provided new ideas for the identification of rice resistance resources, and promoted a better understanding of pest control.
研究领域:
植物学
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遗传学
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害虫防治
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2023
·Vol. 25
·期 1
·386
·
IF 5.6
Ziyuan Deng,Chengling Lai,Jun Zhang,Fan Sun,Danting Li,Peiying Hao,Xuping Shentu,Kun Pang,Xiaoping Yu
The brown planthopperNilaparvata lugens(Stål) (BPH) is a main rice pest in China and many other Asian countries. In the control of BPH, the application of insect-resistant rice has proven to be quite effective. Secondary metabolites are essential weapons in plants’ defense against phytophagous insects. Studies have found that differences in the content of secondary metabolites play a crucial role in determining whether rice exhibits resistance or susceptibility to BPH. Simultaneously, symbionts are essential to the BPH. Nevertheless, there is limited research on the impact of secondary metabolites on the symbionts within BPH. Therefore, investigating the influence of secondary metabolites on both BPH and their symbionts is significant for the control of BPH. In this experiment, newly emerged female adults of BPH were fed artificial diets containing 10 different secondary metabolites. The results indicated that methyl jasmonate had inhibitory effects on the survival rate, weight gain, and reproductive capacity of BPH. Using qPCR methods, it was discovered that the number of symbiotic fungi (Ascomycetessymbionts) within BPH significantly decreased under methyl jasmonate stress. In conclusion, this experiment has preliminarily revealed the inhibitory effects of methyl jasmonate on BPH and its symbionts, demonstrating its potential for controlling BPH.Keywords:brown planthopper;secondary metabolites;symbionts;methyl jasmonate
研究领域:
植物科学
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昆虫学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Plants-Basel
·
2024
·Vol. 13
·期 11
·1494
·
IF 4
Kaiming Mao,Chengzhe Li,Huacai Zhai,Yuying Wang,Yonggen Lou,Wenhua Xue,Guoxin Zhou
Green leaf volatiles (GLVs) play pivotal roles in plant anti-herbivore defense. This study investigated whether the rice 13-lipoxygense geneOsRCI-1is involved in GLV production and plant defense in rice. The overexpression ofOsRCI-1(oeRCIlines) in rice resulted in increased wound-induced levels of two prominent GLVs,cis-3-hexen-1-ol andcis-3-hexenal. In a previous study, we found that the overexpression ofOsRCI-1reduced the colonization by the rice brown planthopper (BPH,Nilaparvata lugens) but increased the attractiveness to the egg parasitoidAnagrus nilaparvataecompared to wild-type (WT) plants. This study found that whencis-3-hexen-1-ol, but notcis-3-hexenal, was added to WT plants, it could change the BPH’s colonization preference, i.e., more BPHs preferred to colonize theoeRCIlines. The exogenous application ofcis-3-hexen-1-ol orcis-3-hexenal to BPH-infested WT plants could weaken or overturn the preference ofA. nilaparvataeforoeRCIlines. However, field experiments revealed that onlycis-3-hexenal was attractive to the parasitoid and increased the parasitism rates of BPH eggs. These results indicate thatOsRCI-1is involved in rice GLV production and therefore modulates both direct and indirect defense in rice.
研究领域:
植物学
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昆虫学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CROP PROTECTION
·
2024
·
IF 2.5
Zhong-Yan Zhu,Xiao-Bao Wu,Jia-jia An,Qian-Qian Deng,Jun Wang,Li-Na Chen,Mao Ye
This investigation assessed the impact of calcium chloride (CaCl 2 ) seed priming on the direct and indirect defense mechanisms of rice ( Oryza sativa L.) against the brown planthopper (BPH), Nilaparvata lugens (Stål). Our findings revealed that seeds primed with CaCl 2 significantly curtailed the survival and developmental rates of BPH nymphs and diminished the honeydew secretion by adult females. The feeding behaviour of BPH on CaCl 2 -pretreated rice showed an extended non-proboscis handling (np) duration, while the phloem ingestion time (N4-b) and xylem ingestion time (N5) were reduced. Additionally, BPH nymphs exhibited a marked preference for H 2 O-pretreated plants over CaCl 2 -primed ones for both feeding and oviposition. Conversely, the natural enemy Cytorhinus lividipennis displayed a heightened preference for preying on BPH nymphs and eggs on CaCl 2 -primed rice. The treatment with calcium led to an increased release of a diverse range of volatile compounds following BPH infestation, compared to H 2 O pretreatment. Notably, the volatiles Methyl salicylate and 1-octen-3-ol were identified as the most effective deterrents for BPH and attractants for C. lividipennis , respectively, highlighting their potential role in mediating plant-insect interactions.
研究领域:
植物保护
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昆虫学
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农业科学
✓ 已验证
DOI
高影响因子
MOLECULES
·
2020
·Vol. 25
·期 9
·2044
·
IF 3.267
Xiubing Gao,Can Guo,Ming Li,Rongyu Li,Xiaomao Wu,Anlong Hu,Xianfeng Hu,Feixu Mo,Shuai Wu
The brown planthopper (BPH), Nilaparvata lugens (Stål), is the most notorious rice insect pest. In order to repel BPH effectively while being environmentally friendly, a new film based on guar gum incorporated with citral (GC film) was formulated. A toxicity bioassay of citral and guar gum at different proportions (ratios of 3:1, 2:1, 1:1, 1:2, and 1:3 inw/w) of GC film-forming emulsion to BPH was performed with the rice stem dipping method. Results showed that the most effective ratio of citral to guar gum was 1:1 with the median lethal concentration (LC50) of 4.30 mg/mL, far below the LC50of guar gum (GG)/citral individual (141.51 and 44.38 mg/mL, respectively). The mortality of BPH adults and nymphs in the third instar treated with different dilution multiples of GC film-forming emulsion ranged from 46.67% to 82.22% and from 37.78% to 71.11%, respectively. These indicated that GC film-forming emulsion had a direct toxicity on BPH, and the mixture of citral and GG had synergistic interactions. Subsequently, Fourier-transform infrared spectroscopy showed that the incorporation of guar gum with citral was successful and did not result in the formation of new chemical bonds. The GC film exhibited a darker color and rougher surface topography with larger apertures and deeper gullies (Ra = 1.42 nm, Rq = 2.05 nm, and Rmax = 25.40 nm) compared to the guar gum film (GG film) (Ra = 1.00 nm, Rq = 1.33 nm, and Rmax = 16.40 nm), as determined by transmission electron microscopy and atomic force microscopy. The GC film exhibited a 50.4% lower solubility in water (30.30% vs. 15.00%) and 71.3% oxygen permeability (8.26 × 10−9vs. 2.37 × 10−9cm3/m2·d·Pa) (p <0.05) but did not demonstrate any significant difference in mechanical properties, such as thickness (39.10 vs. 41.70 mm), tensile strength (41.89 vs. 38.30 N/mm2), and elongation at break (1.82% vs. 2.03%) (p <0.05) compared to the GG film. Our findings established a link between physicochemical properties and bioactivity, which can provide useful information on developing and improving GC films and may offer an alternative approach for the control of BPH in the near future.
研究领域:
农业昆虫学
·
材料科学
✓ 已验证
DOI
高影响因子
JOURNAL OF PEST SCIENCE
·
2023
·Vol. 96
·期 3
·1213-1227
·
IF 4.8
Zhang Yan-Chao,Yu Zhi-Tao,Gao Yang,Ye Wen-Nan,Peng Yu-Xuan,Zhang Shuai,Gao Cong-Fen
The brown planthopper (BPH), Nilaparvata lugens (Stål), is a major insect pest of rice in Asia and is mainly controlled with insecticides. Dinotefuran has been commercialized for control of N. lugens in China since 2013; however, the characteristics and mechanisms of resistance to dinotefuran are still unclear. Here, we conducted a long-term (from 2013 to 2021) and large geographical scale (11 provinces) resistance monitoring program for dinotefuran in the BPH in China. Before 2020, field populations of N. lugens have developed moderate resistance to dinotefuran, while since 2020, most field populations of N. lugens have developed high resistance. To develop a better resistance management strategy, a dinotefuran-resistant (Din-R) strain of N. lugens was selected in the laboratory to investigate the inheritance, possible mechanisms of resistance and potential fitness costs. This Din-R strain developed resistance to imidacloprid, nitenpyram and thiamethoxam. The dinotefuran resistance was controlled by multiple genes. RNA interference (RNAi)-mediated suppression of NlCYP6ER1 , NlCYP6CS1 and NlCYP314A1 expression increased the susceptibility of N. lugens to dinotefuran. Transgenic lines of Drosophila melanogaster expressing NlCYP6ER1 or NlCYP314A1 were less susceptible to all four neonicotinoids. Furthermore, the Din-R strain had a relative fitness of 0.64 with a substantially decreased survival rate, emergence rate and fecundity. Collectively, our results showed that dinotefuran resistance was mediated by multiple CYP genes. The high expression of these genes was related to the cross-resistance of N. lugens to other neonicotinoids. This study provided valuable information for facilitating the development of neonicotinoids resistance management strategies.
研究领域:
昆虫学
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害虫管理
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分子生物学
✓ 已验证
产品关联
DOI
高影响因子
PEST MANAGEMENT SCIENCE
·
2022
·Vol. 79
·期 1
·125-133
·
IF 4.462
Bin Zeng,Ya-Ting Liu,Ze-Rui Feng,Fu-Rong Chen,Shun-Fan Wu,Chris Bass,Cong-Fen Gao
Background Buprofezin, an insect growth regulator, has been widely used to control brown planthopper (BPH), Nilaparvata lugens , one of the most destructive pests of rice crops in Asia. The intensive use of this compound has resulted in very high levels of resistance to buprofezin in the field, however, the underpinning mechanisms of resistance have not been fully resolved. Results Insecticide bioassays using the P450 inhibitor piperonyl butoxide significantly synergized the toxicity of buprofezin in two resistant strains of BPH (BPR and YC2017) compared to a susceptible strain (Sus), suggesting P450s play a role in resistance to this compound. Whole transcriptome profiling identified 1110 genes that were upregulated in the BPR strain compared to the Sus strain, including 13 cytochrome P450 genes, eight esterases and one glutathione S -transferase. Subsequently, qPCR validation revealed that four of the P450 genes, CYP6ER1vA , CYP6CW1 , CYP4C77 , and CYP439A1 were significantly overexpressed in both the BRP and YC2017 strains compared with the Sus strain. Further functional analyses showed that only suppression of CYP6ER1vA , CYP6CW1 , and CYP439A1 gene expression by RNA interference significantly increased the toxicity of buprofezin against BPH. However, only transgenic Drosophila melanogaster expressing CYP6ER1vA and CYP439A1 exhibited significant resistance to buprofezin. Finally, the BPR strain was found to exhibit modest but significant levels of resistance to acetamiprid, dinotefuran and pymetrozine. Conclusions Our findings provide strong evidence that the overexpression of CYP6ER1vA and CYP439A1 contribute to buprofezin resistance in BPH, and that resistance to this compound is associated with low-level resistance to acetamiprid, dinotefuran and pymetrozine. These results advance understanding of the molecular basis of BPH resistance to buprofezin and will inform the development of management strategies for the control of this highly damaging pest. © 2022 Society of Chemical Industry.
研究领域:
昆虫学
·
分子生物学
·
害虫管理
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Agronomy-Basel
·
2024
·Vol. 14
·期 11
·2566
·
IF 3.3
Ao Li,Peiwen Zhu,Deyan Kong,Lei Wang,Anning Zhang,Yi Liu,Xinqiao Yu,Lijun Luo,Feiming Wang
Rice is a major global staple crop, but rising temperatures and freshwater shortages have made drought one of the most severe abiotic stresses affecting agriculture. Additionally, rice blast disease and brown planthopper infestations significantly impact yields. Therefore, developing water-saving, drought-resistant, high-yielding, and disease-resistant rice varieties is critical for sustainable rice production. The new water-saving and drought-resistant (WDR) rice ‘Huhan 1516’, bred using marker-assisted selection (MAS) and marker-assisted backcrossing (MABC) techniques, addresses these challenges. This variety is highly adaptable to drought-prone and water-scarce regions such as the Yangtze and Huai River basins. With its high yield, drought tolerance, and broad-spectrum resistance to rice blast (conferred by thePi2gene) and brown planthopper (BPH), ‘Huhan 1516’ is suitable for various farming systems and environments. Field trials show that this variety outperforms control varieties by 2.2% in yield and exhibits moderate resistance to both rice blast and brown planthopper. ‘Huhan 1516’ has been recognized as a new water-saving and drought-resistant rice variety by the state, and as a released cultivar, it has great potential for market promotion and application.
研究领域:
植物学
·
遗传学
·
农学
✓ 已验证
DOI
高影响因子
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
·
2018
·Vol. 115
·期 20
·5175-5180
·
IF 9.504
Peng-Lu Pan,Yu-Xuan Ye,Yi-Han Lou,Jia-Bao Lu,Chen Cheng,Yan Shen,Bernard Moussian,Chuan-Xi Zhang
Cuticle, mainly composed of chitin and cuticular proteins (CPs), is a multifunctional structure of arthropods. CPs usually account for >1% of the total insect proteins. Why does an insect encode so many different CP genes in the genome? In this study, we use comprehensive large-scale technologies to study the full complement of CPs (i.e., the CP-ome) of the brown planthopper (BPH), Nilaparvata lugens, a major rice plant pest. Eight CP families (CPR, CPF, TWDL, CPLCP, CPG, CPAP1, CPAP3, and CPAPn) including 140 proteins in BPH, in which CPAPn is a CP family that we discovered. The CPG family that was considered to be restricted to the Lepidoptera has also been identified in BPH. As reported here, CPLCP family members are characterized by three conserved sequence motifs. In addition, we identified a testis protein family with a peritrophin A domain that we named TPAP. We authenticated the real existence of 106 proteins among the 140 CPs. RNA interference (RNAi) experiments were conducted against 135 CP genes in early- and late-instar nymphs and newly emerged female adults, demonstrating that 32 CPs were essential for BPH normal development or egg production. Combined RNAi experiments suggested redundant and complementary functions of the large number of CPs. Transcriptomic data revealed that the CP genes were expressed in a tissue-specific manner, and there were four clusters of developmental expression patterns. This study gives a comprehensive understanding of the roles of CPs in an insect cuticle.
研究领域:
昆虫学
·
基因组学
·
蛋白质组学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Frontiers in Physiology
·
2019
·
IF 3.201
Shentu Xuping,Wang Xiaolong,Xiao Yin,Yu Xiaoping
Yeast-like symbiotes (YLS), harbored in the abdomen fat-body cells of the rice brown planthopper (BPH), Nilaparvata lugens Stål (Hemiptera: Delphacidae), are vital to the growth and reproduction of their host. It is feasible to manipulate BPH infestation on rice by inhibiting YLS using fungicide. In this study, the fungicide propiconazole was injected into the hemolymph of BPH thorax via microinjection to investigate its effect on YLS, especially the dominant species, Hypomyces chrysospermus, and their host BPH. Propiconazole markedly reduced the total number of YLS and H. chrysospermus in BPH hemolymph and fat body, thereby leading to an obvious higher mortality and lower fecundity of BPH than the negative control (PBS, phosphate buffer solution). After microinjecting propiconazole, the survival rate of BPH nymphs at the 5th instar was significantly lower than that obtained after PBS treatment. Eight days after propiconazole microinjection, the BPH survival rate dropped to 40%, only half of BPH survival rate treated with PBS microinjection. For female adults (1-day-old), there were significant differences in the survival rates between BPHs treated with propiconazole and those treated with PBS at days 5–8. The fecundity of BPH decreased significantly by microinjecting propiconazole and averaged only 229 eggs per female, which was 20% less than that of the negative control. Furthermore, we reared BPH on the susceptible variety TN1 sprayed with propiconazole to prove the feasibility manipulating field occurrence of BPH by inhibiting YLS using fungicides. The number of YLS and H. chrysospermus in BPH obviously declined. Subsequently, the survival rate and fecundity of BPH significantly decreased after feeding on rice treated with propiconazole. Meanwhile, the propiconazole residue was detected in the hemolymph and gut of BPH by HPLC analysis within 1 day of feeding. Inhibiting YLS using fungicides was a novel and effective way to control BPH infestation.
研究领域:
昆虫学
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植物保护
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2021
·Vol. 77
·期 9
·4128-4137
·
IF 4.845
Li-Xiang Wang,Sha Tao,Yan Zhang,Ya-Long Jia,Shun-Fan Wu,Cong-Fen Gao
BACKGROUND Pymetrozine is commonly used for the control of Nilaparvata lugens , and resistance to pymetrozine has been frequently reported in the field populations in recent years. However, the mechanism of brown planthopper resistance to pymetrozine is still unknown. RESULTS In this study, a pymetrozine-resistant strain (PMR) was established, and the potential biochemical resistance mechanism of N. lugens to pymetrozine was investigated. Pymetrozine was synergized by the inhibitor piperonyl butoxide (PBO) in the PMR with 2.83-fold relative synergistic ratios compared with the susceptible strain (Sus). Compared with the Sus, the cytochrome P450 monooxygenase activity of PMR was increased by 1.7 times, and two P450 genes ( NlCYP6CS1 and NlCYP301B1 ) were found to be significantly overexpressed more than 6.0-fold in the PMR. Pymetrozine exposure induced upregulation of NlCYP6CS1 expression in the Sus, but the expression of NlCYP301B1 did not change significantly. In addition, RNA interference (RNAi)-mediated suppression of NlCYP6CS1 gene expression dramatically increased the toxicity of pymetrozine against N. lugens . Moreover, transgenic lines of Drosophila melanogaster expressing NlCYP6CS1 were less susceptible to pymetrozine, and had a stronger ability to metabolize pymetrozine. CONCLUSIONS Taken together, our findings indicate that the overexpression of NlCYP6CS1 is one of the key factors contributing to pymetrozine resistance in N. lugens . And this result is helpful in proposing a management strategy for pymetrozine resistance.
研究领域:
昆虫学
·
分子生物学
·
害虫管理
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）