ACS Applied Nano Materials
·
2026
·
IF 5.8
Yao Zhang,Hui Zhang,Ziyao Jia,Chunya Li,Lianqing Chen,Shiwei Jin
Surface-enhanced Raman scattering (SERS) holds significant potential for rapid food safety screening; however, its application is often constrained by substrate sensitivity, stability, and cost. Here, we developed a multifunctional SERS substrate by integrating a 3D-printed microstructured template with a slippery liquid-infused porous surface (SLIPS) and Ag/UiO-66 metal–organic framework (MOF) nanoparticles for food safety monitoring. The nearly pinning-free SLIPS interface enabled evaporation-driven enrichment of nanoparticles at microcone tips, generating dense, spatially confined hotspots with an enhancement factor (EF) of 1.44 × 108. The Ag/UiO-66@SLIPS substrate demonstrated ultrasensitive SERS detection of illicit sildenafil (SD) and food-dye additives (using rhodamine 6G as a model). The substrate achieved limits of detection (LOD) for SD of 100 pg/mL (2.11 × 10–10 M) in ethanol and 1 ng/mL (2.11 × 10–9 M) in health wine using only 10 μL samples. For rhodamine 6G (R6G), an ultralow LOD of 1 fg/mL (2.09 × 10–15 M) was obtained. Furthermore, for photocatalytically degradable dyes, the inherent catalytic activity of Ag/UiO-66 provided a self-cleaning pathway to mitigate signal carryover and enabled repeated measurements. The substrate maintained 83.5% signal intensity over 150 days and can be refreshed for continued use. This work combines biomimetic structures with functionalized nanomaterials, enabling the simple, cost-effective development of robust, uniform SERS substrates for multiscenario trace molecular sensing in food safety.
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高影响因子
ANALYST
·
2021
·Vol. 146
·期 12
·4080-4086
·
IF 4.616
Hao Gu,Kang Ma,Weiqian Zhao,Lirong Qiu,Wei Xu
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) has been widely applied for the analysis of large biomolecules. The emergence of inorganic material substrates and new organic matrices extends the use of MALDI MS for small molecule analyses. However, there are usually preferred matrices for different types of analytes. Here, an organic compound, 4-hydroxy-3-nitrobenzonitrile, was found to be a general purpose matrix for the analyses of small organic, peptide and protein molecules. In particular, 4-hydroxy-3-nitrobenzonitrile has a strong UV absorption property, and it provides a clean background in the low mass range. Its analytical performances as a UV-laser matrix were demonstrated for different types of analytes, including organic drugs, peptides, proteins, mouse brain tissue and bacteria. Compared with commercial matrices, this new matrix has better performances when analyzing small molecules, such as drugs, peptides and lipids, while it has similar performances when analyzing proteins.
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PubMed
高影响因子
JOURNAL OF PHYSICS D-APPLIED PHYSICS
·
2022
·Vol. 55
·期 48
·485202
·
IF 3.409
Kun Liu,Xiaohan Zhang,Xiongfeng Zhou,Xinming Huo,Xiaohao Wang,Kostya (Ken) Ostrikov
This work reported an air atmospheric pressure plasma jet served as an ion source for nonvolatile solid analysis, which allows a unique capability to achieve thermal desorption and ionization simultaneously without complex devices, inert gas, or solvents. Owing to precise amount of heat generated by plasma jet, nonvolatile analytes were thermally released from solid surfaces without the assistance of secondary desorption equipment. According to the mass spectra and Fourier transform infrared spectrum, [M + H]+ and [M + NO3]− ions were the major analyte ions in the positive-ion and negative-ion modes, respectively. The applied voltages, discharge currents, and gas temperatures of plasma jet were measured, and found to be positively correlated with the mass spectra signal intensity of the samples, while the added resistances and gas flow rate were negatively correlated with the signal intensity. The influence mechanisms of analytes natures coupling physical parameters of plasma jet on detection performance were revealed. The prospect of quantitative testing was confirmed by the linear relationship between the peak intensity and sample mass.
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高影响因子
ASIAN JOURNAL OF ANDROLOGY
·
2023
·
IF 2.9
Yang Yi-Ting,Yan Bin,Li Yu-Hua,Guo Li-Na,Wang Wei-Wei,Liu Li-Jie,Yu He-Guo,Diao Hua
Phosphodiesterase (PDE) inhibitors can improve sperm motility in patients with asthenozoospermia. However, the most commonly reported nonselective PDE inhibitor pentoxifylline and PDE5 inhibitor sildenafil have the disadvantages of requiring a high concentration and destroying sperm integrity. We examined the PDE10A inhibitor PF-2545920 to compare its ability to promote sperm motility with that of pentoxifylline and sildenafil . After seminal plasma was discarded, several semen samples were subjected to four treatments (control, PF-2545920, pentoxifylline , and sildenafil ) to evaluate their ability to affect motility, viability, and spontaneous acrosome reactions. Intracellular calcium and adenosine triphosphate (ATP), mitochondrial membrane potential, and penetration through viscous medium were assessed by flow cytometry, luciferase, and hyaluronic acid after treatment with PF-2545920. Statistical analyses were performed using the analysis of variance statistical test. PF-2545920 elevated the percentage of motile spermatozoa compared to the control, pentoxifylline , and sildenafil groups at 10 µmol l −1 ( P < 0.01). It is less toxic to GC-2spd mouse spermatocytes cells and spermatozoa and causes fewer spontaneous acrosomal reactions ( P < 0.05). PF-2545920 also increased mitochondrial membrane potential ( P < 0.001) and altered intracellular calcium ( P < 0.05) in a dose-dependent manner, including increasing sperm hyaluronic acid penetrating ability ( P < 0.05). Therefore, PF-2545920 might be an excellent choice for stimulating the sperm motility .
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PubMed
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2019
·
IF 0.931
Ting-Ting ZOU,Jin-Yi YANG,Zhen-Lin XU,Hong WANG,Yuan-Ming SUN,Hong-Tao LEI,Xue-Cai TAN,Yu-Dong SHEN
To monitor illegal addition of tadalafil and its analogues in health food, a new polyclonal antibody was prepared by immunizing New Zealand white rabbits with a common skeleton structure hapten combined with an unsaturated linear alkyl arm hapten strategy, which could simultaneously recognize tadalafil, amino tadalafil and N -Desmethyl tadalafil. A microwell lateral flow immune- chromatographic assay ( mw LFIA) method combined with simple sample pretreatment was developed. The mw LFIA showed that the LOD for three tadalafil drugs was between 0.05 ng mL −1 and 0.30 ng mL −1 , the cut-off values were between 50 ng mL −1 and 100 ng mL −1 . Cross-reactivity (CR) results showed no interference from substances with similar structures, indicating a good specificity against tadalafil, amino tadalafil and N -Desmethyl tadalafil. The average recoveries of three drugs in spiked health wine and oral liquid ranged from 78.7% to 117.8% with an RSD of less than 15%, showing a good aggreement with the results of HPLC-MS/MS method. The developed mw LFIA strip was suitable for the rapid detection of tadalafil and its analogues in health wine and oral liquid samples.
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高影响因子
Food Bioscience
·
2024
·
IF 4.8
Tian Wei,Yongyi Zhang,Jianxin Huang,Jinyi Yang,Yuanxin Tian,Yudong Shen
In recent years, phosphodiesterase type 5 enzyme inhibitors, including tadalafil, sildenafil, and their analogues have emerged as illegal ingredients in dietary supplements. It can cause serious clinical side effects in consumers, resulting in diverse symptoms. To rapidly screen illegal drugs in dietary supplements, a dual-labeled probe time-resolved fluorescence immunochromatography assay (DL-TRFICA) based on two broad-spectrum antibodies labeled with time-resolved fluorescent microspheres was established for the simultaneous qualitative and quantitative detection of nine sildenafil-like and three tadalafil-like adulterants. The half-maximal inhibitory concentration (IC 50 ) of sildenafil-like and tadalafil-like adulterants were 2.49–5.88 and 2.15–2.78 ng mL −1 , the limit of detection were 0.08–1.66 and 0.59–1.85 ng mL −1 , and the visualization value (cut-off value) were 31.25–250 and 50–62.50 ng mL −1 , respectively. Notably, the potential practical applications of DL-TRFICA evaluated by conducting a quantitative evaluation of sildenafil and tadalafil adulterants in dietary supplement samples (herbal spirit drinks, healthcare oral liquids, and herbal medicine pills) demonstrated a recovery rate ranging from 80.0% to 125.8% with a coefficient of variation of less than 15%, which concluded that the DL-TRFICA approach could be used in real sample detection.
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高影响因子
危险品化学品经营许可证（带存储）