JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Muhammad Noman,Azizullah,Temoor Ahmed,Yizhou Gao,Hui Wang,Xiaohui Xiong,Jiajing Wang,Jiajun Lou,Dayong Li,Fengming Song
The ubiquitin–proteasome system (UPS) regulates protein quality or control and plays essential roles in several biological and biochemical processes in fungi. Here, we present the characterization of two UPS components, FonDoa1 and FonDoa4, in watermelon Fusarium wilt fungus, Fusarium oxysporum f. sp. niveum (Fon), and their biological functions. FonDoa1 localizes in both the nucleus and cytoplasm, while FonDoa4 is predominantly present in the cytoplasm. Both genes show higher expression in germinating macroconidia at 12 h. Deletion of FonDoa1 or FonDoa4 affects vegetative growth, conidiation, conidial germination/morphology, apoptosis, and responses to environmental stressors. FonDoa1, but not FonDoa4, positively regulates autophagy. The targeted disruption mutants exhibit significantly attenuated pathogenicity on watermelon due to defects in the infection process and invasive fungal growth. Further results indicate that the WD40, PFU, and PUL domains are essential for the function of FonDoa1 in Fon pathogenicity and environmental stress responses. These findings demonstrate the previously uncharacterized biological functions of FonDoa1 and FonDoa4 in phytopathogenic fungi, providing potential targets for developing strategies to control watermelon Fusarium wilt.
研究领域:
真菌学
·
植物病理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2024
·
IF 3.7
Lu Wang,Fengyu Li,Lingtao Meng,Kai Wang,Wenying Li,Fangming Fan,Xiaobing Zhang,Xinyue Jiang,Wei Mu,Xiuyu Pang
During production, agricultural products are often susceptible to potential harm caused by residual traces of pesticides. Oxine-copper is a broad spectrum and efficient protective fungicide widely used in the production of fruits and vegetables. The present study was carried out to profile the dissipation behaviors and residues of oxine-copper on cucumber and watermelon using QuEChERS pretreatment and UPLC–MS/MS. Its storage stability and dietary risk assessment were also estimated. The method validation displayed good linearity (R2 ≥ 0.9980), sensitivity (limits of quantification ≤0.01 mg/kg), and recoveries (75.5–95.8%) with relative standard deviations of 2.27–8.26%. According to first-order kinetics, the half-lives of oxine-copper in cucumber and watermelon were 1.77–2.11 and 3.57–4.68 d, respectively. The terminal residues of oxine-copper in cucumber and watermelon samples were within <0.01–0.264 and <0.01–0.0641 mg/kg, respectively. Based on dietary risk assessment, the estimated long-term dietary risk probability value of oxine-copper in cucumber and watermelon is 64.11%, indicating that long-term consumption of cucumber and watermelon contaminated with oxine-copper would not pose dietary risks to the general population. The results provide scientific guidance for the rational utilization of oxine-copper in field ecosystems of cucumber and watermelon.
研究领域:
农业科学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PEST SCIENCE
·
2024
·Vol. 97
·期 4
·2019-2034
·
IF 4.3
Zhang Yongqian,Zhang Yue,Chen Aiqing,Huo Ran,Yan Hongyu,Zhang Zhiyong,Guo Honggang
Cotton aphids ( Aphis gossypii Glover) are important pests of cucurbit crops. Plant defenses at different cell layers, including the leaf surface, mesophyll cells, and phloem, are employed to defend aphids. Here, we assessed differences in aphid resistance among six watermelon varieties and elucidated the defense mechanisms underlying aphid-resistant/susceptible watermelon varieties. The population abundance, offspring number per female, and meantime of phloem-feeding (E2 phase) of aphids were the highest on XiNong (XN), followed by JingXin (JX), TianWang (TW), ZaoJia (ZJ), and MeiFuLai (MFL), and these parameters were the lowest on JinMeiDu (JMD). Further analyses showed that there was no correlation between the aphid resistance of six watermelon varieties and defenses at the leaf surface. For defenses at mesophyll cells, aphid infestation increased salicylic acid (SA) content at 48 h post-infestation (hpi) and ROS accumulation at 6 and 12 hpi in six watermelon varieties. For phloem defenses, aphid infestation increased callose content in JMD plants but decreased callose content in JX, TW, ZJ, MFL, and XN plants at 6, 12, 24, and 48 hpi. Moreover, callose deposition suppressed by 2-deoxy-d-glucose (2-DDG) neutralized the resistance of JMD plants and exacerbated the susceptibility of XN plants to aphids, which exhibited a higher population abundance and E2 phase time. Collectively, phloem defenses regulated by aphid-induced callose deposition were responsible for differences in aphid resistance among the watermelon varieties.
研究领域:
植物学
·
昆虫学
·
植物生理学
✓ 已验证
DOI
高影响因子
RSC Advances
·
2019
·Vol. 9
·期 27
·15289-15297
·
IF 3.049
Ye Liu,Huanlu Song,Xiao Yang,Congcong He
Environmental factors (heat, pH, oxygen, light) can induce significant quality changes in watermelon juice during processing. To ascertain the effect of such factors on the quality of watermelon juice, the total soluble solids (TSSs), turbidity, lycopene content, color, and flavor were evaluated during different treatments. The pH had a slight impact on the content of lycopene, but had an obvious impact on turbidity. Heat, oxygen, and illumination had considerable effects on the color of watermelon juice, and the results were visible. The content of aldehydes [hexanal, nonanal, (E)-2-noneal, (Z)-6-nonenal] and ketones (6-methyl-5-hepten-2-one, geranylacetone, β-ionone) decreased in treated watermelon juices, while those of 1-nonanol, (Z)-3-nonen-1-ol and (E,Z)-3,6-nonadien-ol increased during illumination. The order of influence of environmental factors on watermelon-juice quality was light > pH > oxygen > heat.
研究领域:
食品科学
·
植物学
✓ 已验证
DOI
PubMed
高影响因子
Nature Communications
·
2024
·Vol. 15
·期 1
·1-13
·
IF 14.7
Liu Qinglu,Tang Tang,Tian Ziyu,Ding Shiwen,Wang Linqin,Chen Dexin,Wang Zhiwei,Zheng Wentao,Lee Husileng,Lu Xingyu,Miao Xiaohe,Liu Lin,Sun Licheng
Ion-solvating membranes have been gaining increasing attention as core components of electrochemical energy conversion and storage devices. However, the development of ion-solvating membranes with low ion resistance and high ion selectivity still poses challenges. In order to propose an effective strategy for high-performance ion-solvating membranes, this study conducted a comprehensive investigation on watermelon skin membranes through a combination of experimental research and molecular dynamics simulation. The micropores and continuous hydrogen-bonding networks constructed by the synergistic effect of cellulose fiber and pectin enable the hypodermis of watermelon skin membranes to have a high ion conductivity of 282.3 mS cm −1 (room temperature, saturated with 1 M KOH). The negatively charged groups and hydroxyl groups on the microporous channels increase the formate penetration resistance of watermelon skin membranes in contrast to commercially available membranes, and this is crucial for CO 2 electroreduction. Therefore, the confinement of proton donors and negatively charged groups within three-dimensional microporous polymers gives inspiration for the design of high-performance ion-solvating membranes.
研究领域:
材料科学
·
电化学
·
分子动力学模拟
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Sustainable Systems
·
2022
·Vol. 6
·期 10
·2200215
·
IF 6.737
Heng Wang,Shijie Liu,Yujian He,Xiangxin Tian,Jingquan Liu
Efficient utilization of solar energy for solar steam generation is an effective way to tackle the shortage of fresh water resources. However, the utilization of solar steam evaporators often suffers from a series of problems, such as high cost, complex processes, and low conversion rate, which seriously hinders the development of solar steam devices. Here, a polypyrrole/watermelon pulp (WPPY) evaporator, which is based on soaking freeze-dried lumpy watermelon pulp (WP) in pyrrole solution, initiating polymerization in the ice bath, followed by heat-treatment to achieve an efficient solar steam generation (SSG) device, is designed and produced. The WPPY with a pyrrole to WP mass ratio of 1:2 (WPPY-3) exhibits a developed porous structure, superior hydrophilicity, relatively small thermal conductivity (0.0375 W m −1 K −1 , a simple one-step fabrication process, and superior light absorption (94.7%). Its evaporation rate reaches 1.64 kg m −2 h −1 without additional insulation, while the light-to-heat conversion efficiency reaches 96.1% under one solar irradiation (1 kW m −2 ) at the same time. Additionally, it also shows good durability. This environmentally friendly and stable photothermal conversion composite material that shows good cycle stability has great potential for applications in seawater desalination via SSG.
研究领域:
材料科学
·
能源技术
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
ENVIRONMENTAL MICROBIOLOGY
·
2021
·Vol. 24
·期 3
·1200-1220
·
IF 5.491
Yizhou Gao,Xiaohui Xiong,Hui Wang,Jiajing Wang,Yan Bi,Yuqing Yan,Zhongye Cao,Dayong Li,Fengming Song
Summary Fusarium oxysporum f. sp. niveum ( Fon ) is a soil-borne fungus causing vascular Fusarium wilt on watermelon; however, the molecular network regulating Fon virulence remains to be elucidated. Here, we report the function and mechanism of nucleotide sugar transporters (Nsts) in Fon . Fon genome harbours nine FonNst genes with distinct functions in vegetative growth, asexual production, cell wall stress response and virulence. FonNst2 and FonNst3 are required for full virulence of Fon on watermelon and FonNst2 is mainly involved in fungal colonization of the plant tissues. FonNst2 and FonNst3 form homo- or hetero-dimers but function independently in Fon virulence. FonNst2, which has UDP-galactose transporter activity in yeast, interacts with FonEro1 and FonPdi1, both of which are required for full virulence of Fon . FonNst2, FonPdi1 and FonEro1 target to endoplasmic reticulum (ER) and are essential for ER homeostasis and function. FonEro1-FonPdi1 module catalyses the dimerization of FonNst2, which is critical for Fon virulence. Undimerized FonNst2 is unstable and degraded via ER-associated protein degradation in vivo . These data demonstrate that FonEro1-FonPdi1 module-catalysed dimerization of FonNst2 is critical for Fon virulence on watermelon and provide new insights into the regulation of virulence in plant fungal pathogens via disulfide bond formation of key pathogenicity factors.
研究领域:
植物病理学
·
分子生物学
·
真菌遗传学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Artificial Intelligence in Agriculture
·
2025
·
IF 12.4
Zhizhong Sun,Jie Yang,Yang Yao,Dong Hu,Yibin Ying,Junxian Guo,Lijuan Xie
Visible/near-infrared (Vis/NIR) spectroscopy technology has been extensively utilized for the determination of soluble solids content (SSC) in fruits. Nonetheless, the spectral distortion resulting from temperature variations in the sample leads to a decrease in detection accuracy. To mitigate the influence of temperature fluctuations on the accuracy of SSC detection in fruits, using watermelon as an example, this study presents a knowledge-guided temperature correction method utilizing one-dimensional convolutional neural networks (1D-CNN). This method consists of two stages: the first stage involves utilizing 1D-CNN models and gradient-weighted class activation mapping (Grad-CAM) method to acquire gradient-weighted features correlating with temperature. The second stage involves mapping these features and integrating them with the original Vis/NIR spectrum, and then train and test the partial least squares (PLS) model. This knowledge-guided method can identify wavelength bands with high temperature correlation in the Vis/NIR spectra, offering valuable guidance for spectral data processing. The performance of the PLS model constructed using the 15 °C spectrum guided by this method is superior to that of the global model, and can reduce the root mean square error of the prediction set (RMSEP) to 0.324°Brix, which is 32.5 % lower than the RMSEP of the global model (0.480°Brix). The method proposed in this study has superior temperature correction effects than slope and bias correction, piecewise direct standardization, and external parameter orthogonalization correction methods. The results indicate that the knowledge-guided temperature correction method based on deep learning can significantly enhance the detection accuracy of SSC in watermelon, providing valuable reference for the development of PLS calibration methods.
研究领域:
食品科学
·
光谱学
·
人工智能
✓ 已验证
产品关联
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2018
·
IF 3.52
Ye Liu,Congcong He,Huanlu Song
Qualitative and quantitative analysis and odor active value (OAV) calculation were applied to identify the aroma-active compounds of fresh watermelon juice in five varieties: Seedless, Jingxin, Lianfa, Qilin, and Texiaofeng. Principal component analysis and cluster analysis were selected to compare the similarity and difference among watermelons juices. Fifty-five volatiles were identified in watermelon juice in five varieties, among which 6 volatiles were identified for the first time in watermelon. ( Z )-6-nonenal, ( E , Z )-2,6-nonadienal, ( E )-2-nonenal, and ( E , E )-2,4-nonadienal contributed greater to aroma profiles than alcohols due to lower threshold through odor active values. It was indicated by principal component analysis and cluster analysis that watermelon juices were separated into three groups, Jingxin, Lianfa-Texiaofeng, and Seedless-Qilin, whose characteristic volatiles were aldehydes, alcohols, and ketones respectively. The average distance between JX and the other varieties was higher than that of others through cluster analysis, indicating that the aroma of Jingxin differed from that of the other four varieties greatly.
研究领域:
食品科学
·
植物学
✓ 已验证
DOI
高影响因子
Food Analytical Methods
·
2018
·Vol. 11
·期 6
·1677-1689
·
IF 2.245
Liu Ye,He Congcong,Song HuanLu
The aim of the study was to compare the extraction efficiency of headspace and solvent extraction methods on watermelon juice flavor. Solid-phase microextraction (SPME) and solvent-assisted flavor evaporation (SAFE) techniques were employed to extract the volatiles from watermelon juice. Aroma compounds were separated and analyzed through gas chromatography-olfactometry-mass spectrometry (GC-MS/O) equipped with DB-5 and DB-Wax capillary columns. Forty-seven compounds were identified by SPME (30) and SAFE (28). These compounds included 7 aldehydes, 10 olefinic aldehydes, 14 alcohols, 7 sulfur compounds, 5 ketones, and 4 miscellaneous compounds. SPME extracted the saturated aldehydes, olefinic aldehydes, alcohols, and ketones effectively, whereas SAFE exerted better efficiency to extract sulfur compounds. ( Z,Z )-3,6-Nonadienal, ( Z )-6-nonen-1-ol, ( E )-2-hexenal, ( E )-2-nonenal, hexanal, nonanal, ( Z )-3-nonen-1-ol, and ( E )-2-octenal were identified as the key aroma compounds through odor activity value (OAV) and aroma extract dilution analysis (AEDA). Also, the aroma compounds can be extracted comprehensively by the combination of both SPME and SAFE techniques.
研究领域:
食品科学
·
分析化学
✓ 已验证
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2017
·
IF 3.981
Zan Li,Jun Jia,Mingwei Wang,Hua Zhang,Hongyuan Yan,Fengxia Qiao
A C 8 -and-amino-bifunctionalized ordered mesoporous organosilica (C 8 -AMS) was prepared in a deep eutectic solvent—a green solvent—that was used as the reaction medium for this procedure instead of organic solvents or other catalyst for the first time. This method provided a nontoxic way for C 8 grafting and obtaining an ordered mesoporous organosilica material with both hydrophilic and hydrophobic groups. Besides advantages such as its large surface area, regular and uniform pore size, and hydrothermal stability, the obtained bifunctionalized C 8 -AMS also have two main functional groups in the obtained structure, C 8 and amino, which enriched the adsorption mechanism of the material and as a result improved the adsorption selectivity of C 8 -AMS. Its performance as an adsorbent was estimated in extracting trace triazine herbicides from watermelon using solid-phase extraction (SPE) followed by high-performance liquid chromatography (HPLC). The proposed method provided satisfactory features in terms of linearity (0.01–2.00 μg g −1 ), limits of detection (2.9–5.1 ng g −1 ), accuracy (recovery: 85.7–101.7%), and precision (intra-day and inter-day RSDs: 1.68–2.60% and 3.05–4.01%).
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Quality and Safety
·
2022
·
IF 3.949
Liu Yang,Xu Xinxin,Liu Liqiang,Xu Liguang,Kuang Hua,Xu Chuanlai
As a typical triazole fungicide, penconazole (PEN) is widely used in agriculture but has been proven to be toxic. In this study, we designed a new hapten to prepare a highly sensitive and specific anti-PEN monoclonal antibody (mAb) and established a gold nanoparticle-based lateral-flow immunoassay (LFIA) for the detection of PEN residues in watermelon and cucumber. The 50% inhibitory concentration (IC50) of the mAb was 0.42 ng/mL and the LFIA strip had a visual limit of detection (vLOD) of 2.5 ng/g and a cut-off value of 10 ng/g in watermelon and cucumbers. The calculated limit of detection (LOD) of the LFIA strip was 0.36 ng/g for watermelon and 0.29 ng/g for cucumber. The LFIA strip also gave a recovery rate of 92.5%–109.0% for watermelon samples and 92.5%–106.7% for cucumber samples. These results using the LFIA strip are highly consistent with those seen using liquid chromatography–tandem mass spectrometry. Thus our developed LFIA strip represents a potentially reliable tool for the rapid on-site screening for PEN in watermelons and cucumbers.
研究领域:
农业科学
·
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）