Yifan Zhang,Shengxiang Yi,Jie Zhu,Wenjie Yu,Tao Liu,Qiuning Wang,Jingze Cao,Xueyong Huang,Liping Luo
The application of proteomics in the analysis of honey is currently limited. This study systematically investigated the protein characteristics and anti-inflammatory properties of Tibet Plateau Berberis vulgaris honey (BH). Proteomics identified 80 proteins in BH, which showed higher abundance and diversity than low diastase activity kinds of honey, consistent with its diastase activity [36.96 mL/(g·h)]. Metabolomics identified the alkaloid sinomenine as a potential biomarker of BH among 116 compounds. BH extracts (500 μg/mL) significantly inhibited NO release (−31.97 %) and reduced interleukin-1β (IL-1β, −72.16 %) and interleukin-10 (IL-10, −48.87 %) levels in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Cellular metabolomics identified 36 differential metabolites and enriched in the TCA cycle pathway. Molecular docking demonstrated that alkaloid lobeline was associated with high binding energies to isocitrate dehydrogenase (IDH, −8.063 kcal/mol) and pyruvate carboxylase (PC, −8.012 kcal/mol). These findings indicated that BH possessed rich protein diversity and mediated anti-inflammatory effects by modulation the TCA cycle.
Tibetan highland barley fiber (T-Fiber) contains high dietary fiber (DF) and beta-glucose. Highland barley has been shown to reduce the risk of diseases associated with high-fat diets (HFD). In the present research, we studied whether T-Fiber could ameliorate HFD-induced obesity in mice and explored its regulatory effects on intestinal microbes . Our findings revealed that T-Fiber feeding significantly reduced weight gain and dyslipidemia , improved glucose tolerance and insulin resistance, alleviated chronic systemic inflammation, and increased short-chain fatty acids (SCFA) in feces in mice. Histological observations confirmed that T-Fiber alleviated Epididymal fat (EF) and lipid accumulation in the liver. In addition, 16s rRNA sequencing analysis of mice cecum contents showed that T-Fiber restored the HFD-induced gut microbiota. T-Fiber supplementation increased the abundance of potentially beneficial bacteria Muribaculaceae , Akkermansiaceae , and Lachnospiraceae_NK4A136_groups , while significantly reducing the abundance of Bacteroidaceae , Prevotellaceae , Rikenellaceae , and Alloprevotella . Furthermore, the increase in the production of SCFA may be related to the abundance of Lachnospiraceae_NK4A136_group . In conclusion, T-Fiber has some anti-obesity abilities under high-fat feeding and can be developed as a relevant anti-obesity product.
危险品化学品经营许可证（带存储）