Minerals
·
2024
·Vol. 14
·期 4
·352
·
IF 2.2
Zujiang Pan,Cuo Jian,Zaihua Peng,Xinzhuang Fu,Rui He,Tong Yue,Wei Sun
The Yulong copper deposit in Tibet is a typical porphyry copper deposit, with about 30 million tons of copper oxide ore in the surface layer. However, more than 40% of the copper resources are in a combination state, resulting in an extraction efficiency of only 50% for copper via the hydrometallurgical process. In this study, the process mineralogy of the combined copper oxide ore was systematically investigated and a calcium fluoride-enhanced leaching process is proposed to increase the leaching efficiency of the combined copper ore. The process mineralogy of the combined copper oxide ore was analyzed using various testing techniques, including chemical analysis, X-ray diffraction, and a process mineralogy parameter testing system (Mineral Liberation Analysis). The results revealed that limonite accounted for 86.12% of the sample, and 63.51% of the copper resource existed in the form of combined copper oxide in limonite. However, it is difficult for the uniformly distributed combined copper oxide in limonite to sufficiently make contact with sulfuric acid, which is the leaching agent, resulting in low copper leaching efficiency. The enhanced leaching behavior of the combined copper oxide ores was also investigated, thereby determining effective and economical enhanced leaching conditions. Under optimal conditions, at a grinding fineness ratio of −0.074 mm (accounting for 85%), liquid-solid ratio of 4:1, sulfuric acid concentration of 50 g/L, temperature of 30 °C, CaF2dosage of 1% of the ore mass, and leaching time of 4 h, the copper leaching efficiency increased to 60.57%, which was 7.34% higher than that of atmospheric pressure leaching. Finally, the enhanced leaching slag was analyzed using an electron probe microanalyzer. It indicated that fluorine ions can erode the combined copper oxide ore and facilitate the diffusion of hydrogen ions inside the limonite, thereby achieving a strengthening effect.
研究领域:
冶金学
·
矿物学
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DOI
高影响因子
Materials
·
2024
·Vol. 17
·期 21
·5364
·
IF 3.1
Lihui Li,Kaiming Niu,Jianrui Ji,Panpan Zhang,Jilin Zhang
In order to impart the properties of cementitious material to the Tibetan Agar soil, two high-temperature activation mechanisms (HTMA, HTMB) were designed in this study, and the products and hydration-hardening properties of Tibetan Agar soil high-temperature activation mechanism were analyzed by means of SEM, XRD, and XRF. The results show that the main components of Tibetan Aga soil are calcite and quartz; Aga soil is activated by HTMA high-temperature activation, forming the main products of CaO, C2S, CaSiO3, and CaAl2Si2O8, and its products have both air-hardening and water-hardening characteristics; Aga soil is activated by HTMB high-temperature activation, and when the temperature reaches 1250 °C when the clinker is not found in the CaO, the generation of C2S, C3S, C3A, C4AF, and Mg2SiO4minerals with good water-hardening cementitious properties occurs when the temperature rises to 1350 °C, although the formation of some inert minerals that do not have the cementitious properties, but this temperature activation products of the thermodynamic properties of the best; Enhancing the value of lime saturation degree (KH) and silicon rate (SM) can promote the formation of the products of the C2S and C3S, increase the reactivity of the Aga soil activation products, and increase the hydration heat as well as compressive and flexural strength, combined with the results of the hydration heat and mechanical test, KH is recommended to be 0.9~0.94, SM is recommended to be 1.8~2.4, and alumina ratio (IM) is recommended to be 1.8~2.4 when Aga soil is used with raw materials.
研究领域:
材料科学
·
土木工程
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产品关联
DOI
PubMed
高影响因子
MICROSCOPY AND MICROANALYSIS
·
2019
·Vol. 25
·期 3
·822-829
·
IF 2.673
Wu Meng,Zou Xinhui,Zhang Bingjian,Zhao Fan,Xie Zhenbin
Tibetan mural samples from the Jiazhaer mountain cave were studied using enzyme-linked immunosorbent assays (ELISA) and immunofluorescence microscopy (IFM). Samples containing protein binders were first identified using ELISA, and then IFM was used to determine the location of protein binders. Using these methods, we discovered gelatin and casein in samples from wall murals, distributed in both red and black pigments. We excluded the possibility of contamination by conducting further experiments where simulated samples were spiked with milk. We conclude that both gelatin and casein were used as binders in the pigments of the Tibetan Buddhist murals in the Jiazhaer (Transliteration from Tibetan) mountain cave. This is the first evidence of casein being used as a binder in Chinese mural pigments.
研究领域:
考古学
·
免疫学
·
文物保护
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Jianrui Qi,Jie Zhang,Kai Wang,Yifan Cheng,Qinglin Sheng,Ivan Kurtovic,Yahong Yuan,Tianli Yue
There is a lack of research on how Tibetan kefir grains fermentation alters the physicochemical properties and biological activity of Lycium barbarum pulp polysaccharides, despite some reports that fermentation can affect the structure and activity of plant polysaccharides. This study demonstrated that, through fermentation, the molecular weight of polysaccharides decreased from 25.33 to 15.11 kg/mol while the contents of total sugar and uronic acid increased by 19.11% and 40.38%, respectively. Furthermore, after fermentation, the polysaccharides exhibited an uneven and rough surface along with a reduced number of branched chains and triple helix structures. Tibetan kefir grains fermentation enhanced the antioxidant activity of polysaccharides, which may be attributed to an increase in arabinose, galactose, and uronic acid content and a decrease in polysaccharide molecular weight. This research offers an alternative viewpoint on the potential application of Tibetan kefir grains-fermented Lycium barbarum pulp polysaccharides in functional foods.
研究领域:
食品科学
·
营养学
·
生物技术
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产品关联
DOI
PubMed
高影响因子
CHEMISTRY & BIODIVERSITY
·
2022
·Vol. 19
·期 5
·e202100936
·
IF 2.745
Sitong Lu,Zhaoyu Zhang,Youling Liang,Qingkun Zhong,Guangfa Li,Zhang Hu
Phlomis medicinalis Diels, an important perennial herbal plant unique to the Qinghai-Tibet Plateau, is often used as Tibetan Materia Medicine Radix Phlomii for the treatment of cold, cough, and convergence trauma. In order to efficiently extract the iridoid glycosides from P. medicinalis , an ultrasound-assisted deep eutectic solvent extraction technique was employed. The main parameters influencing the extraction process were studied through single-factor tests and the extraction was optimized by using response surface methodology. The hemostasis activity of total iridoid glycosides (TIG) from P. medicinalis was evaluated in vitro and in mice. The optimization results revealed that the optimal process parameters were liquid-solid ratio 20 : 1, choline chloride-lactic acid concentration 79 %, and sonication time 34 min, under which a TIG extraction yield of 20.73 % was obtained. Meanwhile, high-performance liquid chromatography-photodiode array/mass spectrometry (HPLC-PDA/MS) was employed to characterize the optimized extract and indicated that TIG from P. medicinalis mainly consisted of sixteen reported iridoid glycosides with a total content of 91.22 %. The experimental results in vivo and in vitro indicated that TIG from P. medicinalis had strong hemostasis activities, which may be achieved by increasing the fibrinogen levels. Therefore, the ultrasound-assisted deep eutectic solvent extraction is an effective method to extract iridoid glycosides from P. medicinalis and they will be promising candidates to be developed for medical hemostasis agents. Graphical
研究领域:
药用植物学
·
天然产物化学
·
药物开发
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Yifan Zhang,Shengxiang Yi,Jie Zhu,Wenjie Yu,Tao Liu,Qiuning Wang,Jingze Cao,Xueyong Huang,Liping Luo
The application of proteomics in the analysis of honey is currently limited. This study systematically investigated the protein characteristics and anti-inflammatory properties of Tibet Plateau Berberis vulgaris honey (BH). Proteomics identified 80 proteins in BH, which showed higher abundance and diversity than low diastase activity kinds of honey, consistent with its diastase activity [36.96 mL/(g·h)]. Metabolomics identified the alkaloid sinomenine as a potential biomarker of BH among 116 compounds. BH extracts (500 μg/mL) significantly inhibited NO release (−31.97 %) and reduced interleukin-1β (IL-1β, −72.16 %) and interleukin-10 (IL-10, −48.87 %) levels in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Cellular metabolomics identified 36 differential metabolites and enriched in the TCA cycle pathway. Molecular docking demonstrated that alkaloid lobeline was associated with high binding energies to isocitrate dehydrogenase (IDH, −8.063 kcal/mol) and pyruvate carboxylase (PC, −8.012 kcal/mol). These findings indicated that BH possessed rich protein diversity and mediated anti-inflammatory effects by modulation the TCA cycle.
研究领域:
食品科学
·
生物化学
·
免疫学
·
天然产物研究
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2025
·
IF 4.8
Zhanjia Zhang
Introduction: Lactose intolerance affects 85%–95% of Chinese adults, creating substantial demand for low-lactose dairy products. This study aimed to develop a cost-effective β-galactosidase production process through solid-state fermentation (SSF) using agricultural byproducts.Methods: A high-yielding strain was isolated from Tibetan fermented yak milk and identified through morphological, biochemical, and 16S rDNA sequence analysis. Solid-state fermentation conditions were optimized using single-factor experiments and Box-Behnken response surface methodology. Enzymatic properties were characterized, and the enzyme was applied to milk lactose hydrolysis. Techno-economic and environmental impact analyses were conducted.Results: Lactobacillus plantarum LP-15 exhibited initial enzyme activity of 44.7 U/g. Optimal SSF conditions were determined as substrate ratio of wheat bran:soybean meal:whey powder (6:3:1), 37 °C, pH 6.5, and 55% moisture content, achieving enzyme activity of 186.3 U/g (4.17-fold improvement). The enzyme exhibited optimal activity at pH 6.5 and 42 °C, with Km of 2.8 mM and catalytic efficiency of 5.3 × 104 M−1s−1. Milk lactose was reduced by 81.9% within 4 h using 2.0 U/mL enzyme at 40 °C, meeting the low-lactose standard (≤0.1%). SSF reduced production costs by 35.7%, water usage by 94%, and CO2 emissions by 62.4% compared to liquid fermentation.Discussion: This study provides an economically viable and environmentally sustainable solution for low-lactose dairy production in China, establishing independent intellectual property rights while addressing nutritional needs of lactose-intolerant populations through circular utilization of agricultural by products.
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2023
·
IF 5.2
Linyao Gan,Jing Han,Chenyao Li,Jing Tang,Xuebing Wang,Yue Ma,Yefu Chen,Dongguang Xiao,Xuewu Guo
Tibetan highland barley fiber (T-Fiber) contains high dietary fiber (DF) and beta-glucose. Highland barley has been shown to reduce the risk of diseases associated with high-fat diets (HFD). In the present research, we studied whether T-Fiber could ameliorate HFD-induced obesity in mice and explored its regulatory effects on intestinal microbes . Our findings revealed that T-Fiber feeding significantly reduced weight gain and dyslipidemia , improved glucose tolerance and insulin resistance, alleviated chronic systemic inflammation, and increased short-chain fatty acids (SCFA) in feces in mice. Histological observations confirmed that T-Fiber alleviated Epididymal fat (EF) and lipid accumulation in the liver. In addition, 16s rRNA sequencing analysis of mice cecum contents showed that T-Fiber restored the HFD-induced gut microbiota. T-Fiber supplementation increased the abundance of potentially beneficial bacteria Muribaculaceae , Akkermansiaceae , and Lachnospiraceae_NK4A136_groups , while significantly reducing the abundance of Bacteroidaceae , Prevotellaceae , Rikenellaceae , and Alloprevotella . Furthermore, the increase in the production of SCFA may be related to the abundance of Lachnospiraceae_NK4A136_group . In conclusion, T-Fiber has some anti-obesity abilities under high-fat feeding and can be developed as a relevant anti-obesity product.
研究领域:
营养学
·
微生物学
·
代谢学
·
肥胖研究
·
胃肠科学
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产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2022
·
IF 5.895
Yi-Kao Hu,Yi-Ming Liu,Xiao-Lin Bai,Chao Ma,Xun Liao
Fragaria nubicola, known as Tibetan strawberry, is an edible plant possessing various health-promoting effects. However, its functional compositions were rarely studied. In this work, monoamine oxidase B (MAO-B) inhibitors in this plant were rapidly screened using the enzyme-functionalized magnetic nanoparticles coupled with UPLC-QTOF-MS. Two inhibitors, quercetin-3-O-β-d-glucuronide-6″-methyl ester (1) and kaempferol-3-O-β-d-glucuronide-6″-methyl ester (2), were identified from this plant with the IC50 values of 19.44 ± 1.17 and 22.63 ± 1.78 μM, respectively. Enzyme kinetic analysis and molecular docking were carried out to investigate the mechanism of inhibition. Contents of both compounds as well as those of total phenolics and flavonoids were quantified to be 24.76 ± 1.26, 35.59 ± 1.17, 837.67 ± 10.62, and 593.46 ± 10.37 μg/g, respectively. In addition, both compounds exhibited significant neuroprotective effects on 6-hydroxydopamine-induced PC12 cells. This is the first report on the neuroprotective components of F. nubicola, suggesting its potential for developing neuroprotective functional food.
研究领域:
药理学
·
神经科学
·
食品科学
·
植物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）