PROCESS BIOCHEMISTRY
·
2019
·
IF 2.883
Shiling Feng,Haoran Cheng,Zhou Xu,Shican Feng,Ming Yuan,Yan Huang,Jinqiu Liao,Chunbang Ding
Two novel polysaccharides, MRP5 and MRP5A, were obtained from the root of Panax notoginseng using column chromatography. Structure characterization suggested that MRP5A, with an average molecular weight of 11.38 × 10 4 Da, was composed of rhamnose (7.7%), arabinose (6.6%), glucose (69.8%) and galactose (15.9%). MRP5 had an average molecular weight of 9.16 × 10 4 Da and consisted of rhamnose (3.6%), arabinose (14.5%), glucose (51.2%) and galactose (29.7%). The results of methylation analysis and one-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance (NMR) spectra reveal that MRP5 contains repeating units of →3)-β-Glc p -(1→, →6)-β-Glc p -(1→, →3, 6)-Glc p -(1→, β-Glc p- (1→, →3)-β-Gal p- (1→, →3, 6)-β-Gal p -(1→, →3)-α-Rha p -(1→, →3)-α-Ara f -(1→, and α-Ara f -(1→ residues. MRP5 and MRP5A exhibited relatively low antioxidant abilities in vitro , but strongly protective effects against oxidative stress and lifespan extension effects in C. elegans . Overall, the present study indicated that MRP5 could be further investigated as a functional food for promoting healthspan.
研究领域:
植物化学
·
药理学
·
生物化学
✓ 已验证
DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2022
·
IF 3.571
Xueni Zhong,Qianqian Lv,Qiyun Yong,Wenping Hu,Dan Li,Shuhui Ji,Liuyue Zhan,Wenbin Chen,Min Li,Jinsheng Lin,Cuirong Sun
Elagolix sodium (ELS) is a marketed product using to release moderate to severe endometriosis-associated pain. It contains functional groups such as carboxyl group , secondary amino group , 2,4-dioxo pyrimidinyl and several benzyl or benzyl-like position hydrogen atom that are susceptive to occur stress degradation. Forced degradation studies of ELS reveal different degradation profiles of the drug substance which are conducted under photo, thermal, acidic, neutral, alkaline and hydrogen peroxide oxidative conditions in the direction of the ICH guidances. With structural elucidation of LC-PDA/UV-MS n and NMR, the degradants were identified, and seven new degradants are reported in this study. It is confirmed that most of the degradation behaviors of ELS are related to the carboxyl group and secondary amino group in the 3-carboxyl propylamine side chain. Under the oxidative condition using hydrogen peroxide as the oxidant , the secondary amine was oxidized to form an N -hydrogen amine degradant and two further degradants of amine and carbonyl analogs were generated. Under the alkaline degradation condition, the ELS is proven to be stable and no obvious degradants are produced. On the other hand, under the acidic and neutral degradation condition, the 2,4-dioxo pyrimidinyl core of elagolix sodium is stable but the carboxyl group and secondary amine will occur ring cyclization to form the δ -lactam analogs of elagolix sodium. The plausible mechanisms for the degradation of acidic, thermal, photo-degradative and hydrogen peroxide mediated oxidative of elagolix sodium are proposed. It is worth to note that DP-3–4 are the potential degradants which are only found in the solution degradation and are not the real impurities of elagolix sodium.
研究领域:
药物化学
·
分析化学
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH
·
2017
·
IF 1.071
Xing-Peng Hu,Hua Zhou,Yang-Min Du,Shi-Yi Ou,Rian Yan,Yong Wang
The isolation and structure elucidation of two new flavonoids, chrysanthumones A and B from the extracts of Allium chrysanthum were reported. Seven known phenolic constituents were also isolated. Their structures were determined on the basis of NMR and MS techniques.
研究领域:
植物化学
·
天然产物研究
·
药物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2023
·
IF 8.8
Zejun Wang,Zhibing Yang,Yi-Feng Chen
Surfactant-enhanced aquifer remediation has been proved successful to remove dense non-aqueous phase liquids (DNAPLs) from contaminated sites. However, the underlying mechanisms of the DNAPL mobilization and solubilization at the pore scale remains to be addressed for efficient application to the field remediation system. In this work, the emerging microfluidic and imaging technologies are applied to investigate the dynamics of DNAPL remediation. Visualized experiments of the evolution of DNAPL remediation are performed to study the role of surfactant type, concentration and injection rate. The DNAPL remediation is dominated by mobilization followed by solubilization for most surfactants. Mobilization occurs as soon as surfactants and DNAPL are in contact until forming a new stable phase structure, and the solubilization continues until the end of injection. We observe the breakup behavior of long droplets and ganglia during the mobilization, which is attributed to the surfactant-reduced interfacial tension and thus expedites DNAPL mobilization and redistribution. During the solubilization, the formation of micelles incorporating DNAPL fractions increases the DNAPL concentration gradient and thus enhances the mass transfer, but the rate-limited diffusion of micelles reduces the mass transfer rate coefficient. Increasing the surfactant content and decreasing the injection rate can promote mobilization and solubilization. The DNAPL mobilization ability of the surfactants SDS and SDBS is stronger than SAOS and Tween 80 regardless of the injection rates. Tween 80 may be considered an ideal surfactant of only solubilization but not mobilization is desired. This work elucidates the pore-scale mechanisms during surfactant-enhanced DNAPL remediation, which are beneficial for upscaling studies, predictive modeling, and operation optimization of DNAPL remediation in the field.
研究领域:
环境科学
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PHYTOCHEMISTRY
·
2020
·
IF 3.044
Ming Zhou,Xiaogang Peng,Jia Zhou,Ye Liu,Xianggao Meng,Hanli Ruan
Six undescribed triterpenoids , schincarins A‒F, categorized as the preschisanartane (schincarin A), 3,4-secocycloartane (schincarins B‒D), 3,4; 9,10-disecocycloartane (schincarin E), and 3,4-secolanostane (schincarin F) classes, along with twelve known analogues, were obtained from the stems and leaves of Schisandra incarnata Stapf. Their structures were elucidated on the basis of extensive spectroscopic analyses. The absolute configuration of schincarin A was corroborated by analysis of the experimental ECD spectrum, and single-crystal X-ray diffraction with Mo K α irradiation. Schincarin A represented the first example of preschisanartane-type triterpenoid with two oxa-bridged hemiketals . Among the selected compounds which were evaluated for immunosuppressive activities, schincarin C and kadsudilactone A showed potential inhibition against ConA-induced T-cell proliferation with IC 50 values of 5.83 and 6.32 μ M, and LPS-induced B cell proliferation with IC 50 values of 10.21 and 11.49 μ M, respectively.
研究领域:
植物化学
·
药理学
·
有机化学
✓ 已验证
DOI
PubMed
高影响因子
Antibiotics-Basel
·
2024
·Vol. 13
·期 9
·820
·
IF 4.3
Pengfei Sun,Yongyu Tan,Zuhao Zhu,Tinglong Yang,Shalini Thevarajan,Li Zhang
In recent years, the widespread application of antibiotics has raised global concerns, posing a severe threat to ecological health. In this study, the occurrence, source, and ecological risks of 39 antibiotics belonging to 5 classes in mangrove sediments from Lianzhou Bay, China, were assessed. The total concentrations of the antibiotics (∑39 antibiotics) ranged from 65.45 to 202.24 ng/g dry weight (dw), with an average of 142.73 ± 36.76 ng/g dw. The concentrations of these five classes of antibiotics were as follows: Sulfonamides (SAs) > Tetracyclines (TCs) > Fluoroquinolones (QUs) > Penicillin (PCs) > Macrolides (MLs). The spatial distribution of antibiotics varied as high tidal zone > middle tidal zone > low tidal zone. The total organic carbon (TOC), pH, nitrate (NO 3 − -N), and nitrite (NO 2 − -N) of the sediment significantly influenced the distribution of antibiotics ( p < 0.05). A source analysis identified untreated sewage from aquaculture as the primary source of antibiotics in the local mangrove. A risk assessment revealed that ciprofloxacin, norfloxacin, ofloxacin of QUs, and tetracycline of TCs exhibited medium risks to algae in certain sampling sites, while other antibiotics exhibited low or no risks to all organisms. Nevertheless, the total risk of all the detected antibiotics to algae was medium in 95% of the sites. The overall ecological risk level of antibiotics in the middle tidal zone was slightly lower than in the high tidal zone and the lowest in the low tidal zone. In summary, the experimental results provided insights into the fate and transport behaviors of antibiotics in mangrove sediments from Lianzhou Bay.
研究领域:
环境科学
·
生态学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PLANTA MEDICA
·
2014
·
IF 2.339
Hui Xiong,Xin Ding,Xin-zhou Yang,Guang-zhong Yang,Zhi-nan Mei
Ten new triterpene saponins (1–10) have been isolated from the stems of Entada phaseoloides. Their structures were elucidated by spectroscopic analysis and chemical methods. Among these compounds, the aglycons of 6–10 are being reported for the first time, in this study, including 3β,15α,16α-trihydroxy-11α,12α-epoxy olean-28,13β-olide (6), 3β,15α,16α-trihydroxy-11-oxo-olean-12-en-28-oic acids (7 and 8), and 3β,15α,16α-trihydroxy-oleana-11,13(18)-dien-28-oic acids (9 and 10). The cytotoxic activities of all of these compounds were evaluated against HepG-2, A549, and Ec-1 cell lines.
研究领域:
植物化学
·
药理学
·
肿瘤学
✓ 已验证
DOI
PubMed
高影响因子
MATERIALS & DESIGN
·
2025
·
IF 7.9
Mengjiao Jiao,Yang Zhang,Guishang Pei,Zhuoyang Li,Xuewei Lv
This study focused on the high-temperature phase equilibrium of the Fe 2 O 3 -TiO 2 system by employing the classic phase equilibration and quenching method, X-ray diffraction, electron-probe microanalysis, and thermal analysis experiments. The solubility of the hematite, pseudobrookite, and rutile phases as the equilibrium temperature rose from 1100 °C to 1450 °C was determined. Besides, the solubility of TiO 2 in the spinel phase at 1450 °C was verified by experiments. A comprehensive comparison with the phase diagram optimized by Panda indicates that the solubility of solid solutions involved in the Fe 2 O 3 -TiO 2 system should be larger than the predicted one. Additionally, the ordered rutile phase was transformed into the high-temperature rutile-derived crystallographic shear structure at 1400 °C with Fe 2 O 3 higher than 15 mol%. Furthermore, the eutectic reaction of liquid → pseudobrookite + rutile occurred at 1512 °C. The liquidus was determined to be 1512 °C and 1535 °C at 30 and 75 mol% of TiO 2 , respectively. These measured phase equilibrium data are expected to provide reliable input for future thermodynamic reassessments of the Fe 2 O 3 -TiO 2 system within the CALPHAD framework.
研究领域:
材料科学
·
热力学
✓ 已验证
产品关联
DOI
高影响因子
PLANTA MEDICA
·
2016
·
IF 1.99
Min Li,Fen Liu,Yong-Ri Jin,Xiao-Zhong Wang,Qian Wu,Ying Liu,Xu-Wen Li
Five new triterpenoid saponins (1–5) and four known triterpenoid saponins, ginsenoside Re5 (6), majonoside R1 (7), 24(R)-majonoside R1 (8), and ginsenoside Rf (9), were isolated from the rhizomes of Panacis majoris. The structures of new compounds were elucidated as (20S,24S,25R*)-6-O-[β-D-glucopyranosyl-(1 → 2)-β-D-glucopyranosyl]-dammar-20,24-epoxy-3β,6α,12β,25,26-pentaol (1), (20S,24R,25R)-6-O-[β-D-glucopyranosyl-(1 → 2)-β-D-glucopyranosyl]-dammar-20,24-epoxy-3β,6α,12β,25,26-pentaol (2), (20S)-6-O-[β-D-glucopyranosyl-(1 → 2)-β-D-glucopyranosyl]-dammar-20,25-epoxy-3β,6α,12β,24α-tetraol (3), 6-O-[β-D-glucopyranosyl-(1 → 2)-β-D-glucopyranosyl]-dammar-3β,6α,12β,20S,24R,25-hexaol (4), and 6-O-[β-D-glucop-yranosyl-(1 → 2)-β-D-glucopyranosyl]-dammar-25(26)-ene-3β,6α,12β,20S,24R-pentaol (5) on the basis of extensive spectral analysis and chemical methods. Ginsenoside Re5 was isolated from the plant for the first time. The similarities of the nine compounds lie in the fact that their aglycones are conjoined with the same glucopyranose moieties, the same linkage of the glycosyl chains, and the same glycosylation sites, while they have a varied C-17 side chain. Compounds 3 and 5 exhibited moderate antiplatelet aggregation activities induced by adenosine diphosphate with IC50 values of 23.24 and 18.43 µM, respectively. Compound 5 displayed moderate inhibition of arachidonic acid-induced platelet aggregation with an IC50 value of 30.11 µM.
研究领域:
天然产物化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Natural Products and Bioprospecting
·
2018
·Vol. 8
·期 6
·413-418
·
IF 0
Wang Xia,Peng Xing-Rong,Lu Jing,Hu Gui-Lin,Qiu Ming-Hua
Abstract In present study, four new dammarane-type triterpenoids, namely caffruones A–D ( 1 – 4 ), were isolated from the cherries of Coffea arabica . Their structures were elucidated by extensive spectroscopic analysis including 1D, 2D NMR (HSQC, HMBC, 1 H– 1 H COSY, and ROESY), HRMS and IR spectra. This is the first time that tetracyclic triterpenes have been reported in genus Coffea . Graphical Abstract
研究领域:
植物化学
·
天然产物研究
✓ 已验证
产品关联
DOI
PubMed
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2024
·Vol. 25
·期 15
·8479
·
IF 4.9
Yuning Peng,Huanrong Tang,Hao Xiao,Wenyuan Chen,Jingdong Song,Jing Zheng,Hongrong Liu
T5 is a siphophage that has been extensively studied by structural and biochemical methods. However, the complete in situ structures of T5 before and after DNA ejection remain unknown. In this study, we used cryo-electron microscopy (cryo-EM) to determine the structures of mature T5 (a laboratory-adapted, fiberless T5 mutant) and urea-treated empty T5 (lacking the tip complex) at near-atomic resolutions. Atomic models of the head, connector complex, tail tube, and tail tip were built for mature T5, and atomic models of the connector complex, comprising the portal protein pb7, adaptor protein p144, and tail terminator protein p142, were built for urea-treated empty T5. Our findings revealed that the aforementioned proteins did not undergo global conformational changes before and after DNA ejection, indicating that these structural features were conserved among most myophages and siphophages. The present study elucidates the underlying mechanisms of siphophage infection and DNA ejection.
研究领域:
病毒学
·
结构生物学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NATURAL PRODUCT RESEARCH
·
2022
·
IF 2.488
Ruolan Chen,Ziyang Li,Chan Qin,Peijun Lu,Jiaru Lin,Wenxu Zheng,Yahong Xiong,Chunyuan Li
A novel tyroscherin derivative named pseudallecin A (1) with a natural unprecedented morpholine-2, 3-dione structural unit, and a new biogenic synthesis related organic acid named pseudallecin B (2) were purified from a symbiotic fungus Pseudallescheria boydii derived from Pomacea canaliculata. Their structures were elucidated via spectroscopic analyses and ECD calculation. Pseudallecin A exhibited strong inhibitory activities against both Gram-positive Escherichia coli and Gram-negative Staphylococcus aureus.
研究领域:
微生物学
·
有机化学
·
药理学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）