Polymers
·
2022
·Vol. 14
·期 3
·406
·
IF 4.967
Xiao Wang,Xianghong Zheng,Xinyu Liu,Birong Zeng,Yiting Xu,Conghui Yuan,Lizong Dai
Due to unique chelating and macrocyclic effects, crown ether compounds exhibit wide application prospects. They could be introduced into amphiphilic copolymers to provide new trigger mode for drug delivery. In this work, new amphiphilic random polymers of poly(lipoic acid-methacrylate-co-poly(ethylene glycol) methyl ether methacrylate-co-N-isopropylacrylamide-co-benzo-18-crown-6-methacrylamide (abbrev. PLENB) containing a crown ether ring and disulphide bond were synthesized via RAFT polymerization. Using the solvent evaporation method, the PLENB micelles were formed and then used to load substances, such as doxorubicin hydrochloride (DOX) and gold nanoparticles. The results showed that PLENB exhibited a variety of lowest critical solution temperature (LCST) in response to the presence of different ions, such as K+, Na+and Mg2+. In particular, the addition of 150 mM K+increased the LCST of PLENB from 31 to 37 °C and induced the release of DOX from the PLENB@DOX assemblies with a release rate of 99.84% within 12 h under 37 °C. However, Na+and Mg2+ions could not initiate the same response. Furthermore, K+ions drove the disassembly of gold aggregates from the PLENB-SH@Au assemblies to achieve the transport of Au NPs, which is helpful to construct a K+-triggered carrier system.Keywords:crown ether;amphiphilic copolymers;K+-triggered;Au NPs;disassembly
研究领域:
材料科学
·
药物递送
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2022
·
IF 4.132
Ning Chu,Lili Cong,Jing Yue,Weiqing Xu,Shuping Xu
Fluorescent dyes and probes play an indispensable role in bioimaging. The mitochondrion is one of the crucial organelles which takes charge of energy production and is the primary site of aerobic respiration in the cell. To illuminate mitochondria, a series of supramolecular fluorescent imaging probes were developed based on the host–guest assembly of 1,4-bis[2-(4-pyridyl)ethenyl]-benzene (BPEB) derivatives and cucurbituril[6] (CB[6]). These host–guest conjugates can be efficiently internalized into cells due to their water solubility and target mitochondria according to their positive charges. In response to the intracellular microenvironments, these conjugates start dynamic disassembly. The released BPEBs show a highly hydrophobic feature, which can crystallize to form fluorescent solids that illuminate the mitochondria. The intracellular disassembly of the host–guest probes was evidenced by fluorescence lifetime imaging in situ. These smart mitochondrion-targeting fluorescent imaging probes can be available to investigate the structures and functions of mitochondria, which are of great significance in the intracellular dynamic transformation of supramolecular assemblies.
研究领域:
生物化学
·
细胞生物学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2017
·
IF 7.504
Jinlong Ma,Zhenpeng Hu,Wei Wang,Xinyu Wang,Qiang Wu,Zhi Yuan
A reversible programmed targeting strategy could achieve high tumor accumulation due to its long blood circulation time and high cellular internalization. Here, targeting ligand-modified poly(ethylene glycol) (PEG-ligand), dibutylamines (Bu), and pyrrolidinamines (Py) were introduced on the surface of gold nanoparticles (Au NPs) for reversible shielding/deshielding of the targeting ligands by pH-responsive self-assembly. Hydrophobic interaction and steric repulsion are the main driving forces for the self-assembly/disassembly of Au NPs. The precise self-assembly (pH ≥ 7.2) and disassembly (pH ≤ 6.8) of Au NPs with different ligands could be achieved by fine-tuning the modifying molar ratio of Bu and Py (Rm), which followed the formula Rm = 1/(−0.0013X2 + 0.0323X + 1), in which X is the logarithm of the partition coefficient of the targeting ligand. The assembled/disassembled behavior of Au NPs at pH 7.2 and 6.8 was confirmed by transmission electron microscopy and dynamic light scattering. Enzyme-linked immunosorbent assays and cellular uptake studies showed that the ligands could be buried inside the assembly and exposed when disassembled. More importantly, this process was reversible, which provides the possibility of prolonging blood circulation by shielding ligands associated with the NPs that were effused from tumor tissue.
研究领域:
纳米医学
·
生物材料
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2015
·
IF 2.495
Chao Xu,Yong Chen,Heng-Yi Zhang,Yu Liu
A crown ether-based supramolecular gel with a three-dimensional network structure was successfully constructed via a hierarchical induced assembly strategy and characterized by UV/vis NMR, viscometry and DLS. Therein, the coordination linkage of bis(terpyridyl)dibenzo-24-crown-8 1 with Zn 2+ produces the 1 /Zn 2+ supramolecular polymer as the primary assembly. Then, the anthryl-dibenzylammonium guest 2 was grafted to the 1 /Zn 2+ supramolecular polymer via the non-covalent association of dibenzylammonium moieties on guests with the dibenzo-24-crown-8 rings on supramolecular polymer. Furthermore, the supramolecular polymer with anthryl grafts underwent a photo-induced secondary assembly to produce the supramolecular gel, which could be reversibly disassembled by a thermo-induced dissociation of anthracene dimers. These photo/thermo-induced hierarchical assembly/disassembly behaviors will provide a potential way to construct degradable hierarchical supramolecular assemblies employing environment-friendly external stimuli, such as light and heat, as controlling method.
研究领域:
化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
BIOMATERIALS
·
2019
·
IF 10.273
Yanbing Wang,Zhuo Wang,Caina Xu,Huayu Tian,Xuesi Chen
Despite multifunctional nanoparticles using for photothermal therapy can efficiently kill cancer cells, their further application is still hindered by the intrinsic high uptake in the reticuloendothelial system (RES) organs, causing the slow elimination from the body and potential toxicity to the body. Therefore, it is ideal to develop multifunctional nanoparticles which process the ability to effectively accumulate in tumors, while the nanoparticles can be rapidly excreted from the body via renal clearance after effective treatment. Herein, we report the multifunctional nanoparticles (FeTNPs) based on the coordination interaction of phenolic group and metal iron, which are composed of ferric iron, tannic acid (TA) and poly (glutamic acid)- graft -methoxypoly (ethylene glycol) (PLG- g -mPEG). FeTNPs exhibit the following highlighted features: (1) The effective accumulation in the tumor tissue is achieved based on EPR effect. (2) The dual photoacoustic (PA)/magnetic resonance (MR) imaging capacity can provide guidance for the photothermal therapy (PTT). (3) FeTNPs can be dynamically disassembled by deferoxamine mesylate (DFO) to accelerate elimination of the nanoparticles, thus reducing the potential toxicity for the body. The DFO triggered dynamic disassembling strategy may open a new avenue to overcome the dilemma between EPR effect and renal clearance.
研究领域:
生物医学工程
·
癌症治疗
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CONTROLLED RELEASE
·
2017
·
IF 7.786
Zhenpeng Hu,Jinlong Ma,Fei Fu,Chen Cui,Xiaomin Li,Xinyu Wang,Wei Wang,Yeda Wan,Zhi Yuan
Programmed ligand targeting strategy promotes the blood circulation stability of nanoparticles by shielding the ligand. However, the irreversible shielding causes the deshielded nanoparticles to be easily recognized and cleared by the reticuloendothelial system (RES), impeding their further retention in the tumor. Here, we for the first time prove the superiority of the intelligent re-shieldable targeting system that is based on the pH-responsive self-assembly/disassembly of gold nanoparticles . The system can enhance the stability of gold nanoparticles in the blood circulation (2.6-fold at 24 h), reduce uptake by the RES (35% lower) and improve tumor accumulation (41% higher by analysis of gold content in tumor) effectively compared with the conventional irreversible system. Furthermore, preliminary study indicates that the system could be applied as computed tomography contrast agent in tumor imaging. The in vivo validity of the intelligent re-shieldable targeting system provides inspiration for the design of nanomaterials for cancer diagnosis and treatment.
研究领域:
生物医学工程
·
纳米医学
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Applied Materials Today
·
2023
·
IF 8.3
Yuanyuan Chen,Hanqing Dai,Zhe Hu,Jinxin Wei,Wenjie Zhou,Zhongtao Duan,Zhongjie Cui,Wei Wei,Guoqi Zhang,Wanlu Zhang,Ruiqian Guo
Hydrogel materials have biocompatibility, flexibility, transparency, self-healing ability, adhesion with various substrates, anti-freeze ability, and high-temperature resistance. However, the existing hydrogel devices cannot continue to operate in the case of damage, and they cannot work during the repair period, which brings great challenges and threats to life safety. Herein, we have designed a bio-inspired combinable low-power device by imitating the generation of nerve signals whose components can be disassembled and can continue to operate during the period of reconstruction. And the mechanism and determinants of the above phenomena are revealed. The results indicate that this device can establish some information interaction relationships with the body or its surroundings to reflect and identify certain changes, implying that it will possess promising potential in feedback systems, power transformers, intelligence systems, soft robotics, wearable devices , implanted electronics with flexible characteristics matching biological tissues, etc.
研究领域:
生物医学工程
·
材料科学
·
柔性电子
✓ 已验证
产品关联
DOI
高影响因子
Nanoscale
·
2015
·Vol. 7
·期 33
·13918-13923
·
IF 7.394
Wenlong Gao,Mei Liu,Limei Liu,Hui Zhang,Bin Dong,Christopher Y. Li
Although artificial micromotors have undergone tremendous progress in recent years, their fabrication normally requires complex steps or expensive equipment. In this paper, we report a facile one-step method based on an emulsion solvent evaporation process to fabricate multifunctional micromotors. By simultaneously incorporating various components into an oil-in-water droplet, upon emulsification and solidification, a sphere-shaped, asymmetric, and multifunctional micromotor is formed. Some of the attractive functions of this model micromotor include autonomous movement in high ionic strength solution, remote control, enzymatic disassembly and sustained release. This one-step, versatile fabrication method can be easily scaled up and therefore may have great potential in mass production of multifunctional micromotors for a wide range of practical applications.
研究领域:
材料科学
·
化学工程
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of the American Chemical Society
·
2021
·
IF 15.419
Ruo-Can Qian,Ze-Rui Zhou,Weijie Guo,Yuting Wu,Zhenglin Yang,Yi Lu
Regulating cell–cell interactions and cell behaviors via cell surface engineering is of significance for biological research such as cell fate control and cell therapy. While extensive efforts have been made to induce cell–cell assembly via various cell surface modifications triggered by macromolecules or organic metabolites, controllable cell–cell interactions that include both assembly and disassembly triggered by metal ions remain a challenge. Herein, we report a strategy based on DNAzymes to realize controllable cell–cell interactions, triggered by metal ions. The metal-dependent DNAzyme-based cleavage can effectively manipulate cell behaviors, including cell–cell conjunctions and disaggregation. Using a Zn2+-specific DNAzyme, a Mg2+-specific DNAzyme, and their respective substrate strands as the building blocks, the corresponding DNA double-chain switches enabling two-factor disassembly are demonstrated. Moreover, the method has been applied to control the assembly and disassembly between two cell spheroids. Since a wide variety of metal-specific DNAzymes are available, this method can be readily applied to construct cell dynamic systems controlled by other metal ions, providing a smart and versatile platform to regulate dynamic cell behavior.
研究领域:
生物研究
·
细胞治疗
·
分子工程
✓ 已验证
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2025
·
IF 5.5
Yue Cao,Xin Zhang,Bo Ren,Xiaodong Yang
Due to the complex physiological and pathological barriers in tumor tissues, the penetration depth of light is limited and the accumulation and distribution of photosensitizers in the tumor are insufficient, which seriously affects the therapeutic effect of photothermal therapy (PTT) on solid tumors. The preparation process of traditional photothermal agents is complicated, and their biosafety needs further improvement. Moreover, photothermal agents are difficult to rapidly excrete from the body after realizing the therapeutic function, which may cause long-term potential harm to the body. In this study, multifunctional nanoparticles (FeCNPs) consisting of iron(III), curcumin, and poly(ethylene glycol) grafted polyglutamate (PLG-g-mPEG) were obtained through a simple one-pot method. The synthesized FeCNPs exhibited an average hydrodynamic size of 125 nm and a polydispersion index (PDI) of 0.462. Furthermore, the nanoparticle has excellent biocompatibility and a photothermal effect. After the PPT, the FeCNPs break down into harmless elements via a dynamic disassembly mechanism triggered by deferoxamine mesylate (DFO), facilitating swift elimination and preventing possible long-term toxicity to major organs. The therapeutic synergies enable efficient tumor removal. In summary, this study demonstrates an approach for the dynamic disassembly of metal-polyphenol nanoparticles, effectively addressing the challenge of balancing the EPR effect and renal clearance.
研究领域:
肿瘤学
·
纳米医学
·
生物材料学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2020
·
IF 8.758
Wanying Ye,Xiaohui Chen,Xu Li,Yueming Liu,Fan Jia,Qiao Jin,Jian Ji
The size of the nanocarrier is considered one of the most important issues for its therapeutic effect. Thus, an intelligent nanocarrier with dynamic size has been explored as a promising approach to fulfill the requirements for both efficient accumulation according to the enhanced penetration and retention (EPR) effect and deep penetration into tumor tissue. Herein, structure-switchable triplex DNA was modified on gold nanoparticles (AuNPs) to investigate its potential to modulate the nanoparticle dynamic disassembly process among the tumor microenvironment. We report that the pH-sensitive triplex DNA exhibited outstanding sensitivity and size tunability in triggering the disassembly of AuNP clusters into smaller sizes among the tumor acidic environment, leading to better permeability both in vitro and in vivo. By further combination of the telomerase-sensitive hairpin DNA loaded with chemotherapy drug doxorubicin (DOX), a cancer-specific intracellular drug-release function was also realized, resulting in a precise treatment effect and lower toxicity on normal cells. Through comodification of these two structure-switchable DNA chains on AuNPs and construction of nanoparticle assemblies with proper size, programmed disassembly and drug-release function in tissue and cell level, respectively, were successfully combined and eventually facilitated a highly efficient nanodrug transportation process, from tumor accumulation to deep penetration and precise cancer chemotherapy. The study provided the prospect of utilizing functionalized DNA in optimization of nanocarrier delivery efficiency.
研究领域:
纳米医学
·
分子生物学
·
癌症治疗
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2023
·
IF 9.9
Wei Su,Xiantao Jiang,Ying Zhang,Chenxiang Lin,Jun'an Xiao,Peiyuan Li
The disassembly of nanomaterials is of particular interest for high-quality imaging and targeted therapies in the field of nanomedicine. In this study, we developed a novel strategy for fabricating self-assembled naphthalocyanine photosensitizers ( [email protected] ) with intrinsically unique photochemical and photophysical properties. [email protected] could be disassembled under the photothermal effect, and its photoactivity could be enhanced by 780 nm laser irradiation. Moreover, [email protected] generates reactive oxygen species, including superoxide radicals (O 2 • – ) and singlet oxygen ( 1 O 2 ), as well as good photothermal properties, facilitating the application of multifunctional phototherapy. In vitro evaluation indicated that [email protected] possesses an excellent bactericidal effect under a combination of photodynamic (PDT) and photothermal therapy (PTT). The in vivo treatment of a full-layer skin defect model of Escherichia coli ( E. coli ) infection showed that [email protected] had superior antibacterial and wound-healing abilities. These results provide the basis for a feasible strategy to enhance the phototherapeutic effect of photosensitizer (PS) systems.
研究领域:
纳米医学
·
光疗
·
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）