ULTRASONICS SONOCHEMISTRY
·
2016
·
IF 4.556
Fengli Chen,Qiang Zhang,Shimin Fei,Huiyan Gu,Lei Yang
In this study, ultrasonic circulating extraction (UCE) technique was firstly and successfully applied for extraction of samara oil from Acer saccharum . The extraction kinetics were fitted and described, and the extraction mechanism was discussed. Through comparison, n -hexane was selected as the extraction solvent, the influence of solvent type on the responses was detailedly interpreted based on the influence of their properties on the occurrence and intensity of cavitation. Seven parameters potentially influencing the extraction yield of samara oil and content of nervonic acid, including ultrasound irradiation time, ultrasound irradiation power, ultrasound temperature, liquid-solid ratio, soaking time, particle size and stirring rate, were screened through Plackett–Burman design to determine the significant variables. Then, three parameters performed statistically significant, including liquid-solid ratio, ultrasound irradiation time and ultrasound irradiation power, were further optimized using Box–Behnken design to predict optimum extraction conditions. Satisfactory yield of samara oil (11.72 ± 0.38%) and content of nervonic acid (5.28 ± 0.18%) were achieved using the optimal conditions. 1% proportion of ethanol in extraction solvent, 120 °C of drying temperature and 6.4% moisture were selected and applied for effective extraction. There were no distinct differences in the physicochemical properties of samara oil obtained by UCE and Soxhlet extraction, and the samara oil obtained by UCE exhibited better antioxidant activities. Therefore, UCE method has enormous potential for efficient extraction of edible oil with high quality from plant materials.
研究领域:
食品科学与技术
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植物化学工程
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2014
·Vol. 4
·期 79
·42202-42210
·
IF 3.708
Xiao-Ying Qv,Yan-Yun Guo,Jian-Guo Jiang
Bio-energy is arousing the common interest of worldwide researchers to investigate it as a substitute for fossil resources. Compared to other bio-energy sources, algae energy has many advantages. This research was aimed to increase the lipid productivity of Dunaliella tertiolecta, an oleaginous microalgae, by optimizing algal culture medium. Plackett–Burman Design (PBD) was adopted to screen out the critical factors from seven nutritional elements. Box–Behnken design (BBD) was used to seek the optimal nutritional conditions for achieving maximum lipid productivity. Results showed that four nutrients, i.e. NaNO3, NaCl, NaHCO3 and NaH2PO4 2H2O, played a critical role in the accumulation of lipid. GC analysis verified that the four critical nutrients mentioned above had different influences on fatty acid methyl esters, moreover, NaCl had the most prominent effects on fatty acid composition of D. tertiolecta. NaCl 87.69 g L−1, NaNO3 1.77 g L−1, NaH2PO4·2H2O 1.92 g L−1, NaHCO3 5.57 g L−1 were proven to be the optimal culture conditions, under which the lipid productivity in the experiment was 12.576 ± 0.12 mg per L per day, which is approaching the theoretically predicted lipid productivity of 12.925 mg per L per day. The lipid productivity was increased by 31.4% under the optimal culture conditions compared to that under the initial medium composition, indicating that optimization of nutrients could effectively improve the lipid production of D. tertiolecta.
研究领域:
生物能源
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微藻研究
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生物技术
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DOI
高影响因子
Asian Journal of Pharmaceutical Sciences
·
2014
·
IF 0
Shuling Kan,Jing Lu,Jianping Liu,Junlin Wang,Yi Zhao
The study aims to prepare naproxen enteric-coated pellets (NAP-ECPs) by fluid-bed coating using QbD principle. Risk assessment was firstly performed by using failure mode and effect analysis (FMEA) methodology. A Plackett–Burman design was then used for assessment of the most important variables affecting enteric-coated pellets characteristics. A Box–Behnken design was subsequently used for investigating the main, interactive, and quadratic effects of these variables on the response. By FMEA we discovered that eight factors should be considered to be high/important risk variables as compared with others. The responses of acid resistance and cumulative drug release were taken as critical quality attributes (CQAs). Pareto ranking analyses indicated that the coating weight gain (X 7 ), triethyl citrate percentage (X 1 ) and glycerol monostearate percentage (X 2 ) were the most significant factors affecting the selected responses out of the eight high-risk variables. Optimization with response surface method (RSM) further fully clarified the relationship between X 7 , X 1 , X 2 and CQAs, and design space was established based on the constraints set on the responses. Due to the extreme coincidence of the predicted value generated by model with the observed value, the accuracy and robustness of the model were confirmed. It could be concluded that a promising NAP-ECPs was successfully designed using QbD approach in a laboratory scale.
研究领域:
药剂学
·
药物研发
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产品关联
DOI
Biomedicines
·
2023
·Vol. 11
·期 7
·2030
·
IF 4.7
Jie Zhao,Geng Tian,Haibin Qu
The continuous twin-screw wet granulation (TSWG) process was investigated and optimized with prediction-oriented I-optimal designs. The I-optimal designs can not only obtain a precise estimation of the parameters that describe the effect of five input process parameters, including the screw speed, liquid-to-solid (L/S) ratio, TSWG feed rate, and numbers of the 30° and 60° mixing elements, on the granule quality in a TSWG process, but it can also provide a prediction of the response to determine the optimum operating conditions. Based on the constraints of the desired granule properties, a design space for the TSWG was determined, and the ranges of the operating parameters were defined. An acceptable degree of prediction was confirmed through validation experiments, demonstrating the reliability and effectiveness of using the I-optimal design method to study the TSWG process. The I-optimal design method can accelerate the screening and optimization of the TSWG process.Keywords:twin-screw wet granulation (TSWG);I-optimal design;continuous manufacturing;process understanding;design space
研究领域:
制药工程
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化学工程
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产品关联
DOI
PubMed
高影响因子
DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY
·
2023
·
IF 3.4
Yizhe Hou,Xi Wang,Zhiyong Zhang,Jiaheng Wu,Xiang Cai,Pian Li,Zheng Li,Wenlong Li
Objective The drooping process of the Xuesaitong dropping pills (XDPs) was optimized based on quality by design concept. Meanwhile, a machine vision (MV) technology was creatively introduced in this study to predict the critical quality attributes (CQAs) rapidly and accurately.Significance This study improves the understanding of dropping process, and has reference value for the guidance of pharmaceutical process research and industrial production.Methods The study mainly consisted of three stages: the first stage involved the prediction model to establish and evaluate the CQAs, and the second stage involved assessing the quantitative relationships between critical process parameters (CPPs) and CQAs by the mathematical models that were established through the Box–Behnken experimental design. Finally, a probability-based design space for the dropping process was calculated and verified according to the qualification criteria of each quality attribute.Results The results show that the prediction accuracy of the random forest (RF) model was high and met the analysis requirements, and the CQAs of dropping pills can meet the standard by running in the design space.Conclusion The MV technology developed in this study can be applied to the optimization process of the XDPs. In addition, the operation in the design space can not only ensure the quality of XDPs to meet the criteria, but also help to improve the consistency of XDPs.
研究领域:
药学
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工艺优化
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机器学习
·
质量控制
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA B-OPTICAL PHYSICS
·
2024
·Vol. 41
·期 3
·798-808
·
IF 1.8
Shengyi Liu,Duan Gao,Li Wang,Wenbin Song,Zhiliang Zhang,Shitao Wang,Ying Zhu,Qi Zhang
${{\rm Er}^{3 +}}/{{\rm Yb}^{3 +}}$ co-doped ${{\rm SrBi}_4}{{\rm Ti}_4}{{\rm O}_{15}}$ crystalline powders were synthesized using the high-temperature solid-phase method. The crystal structure of the obtained phosphors was analyzed through x-ray diffraction (XRD), confirming the purity of all products such as ${{\rm SrBi}_4}{{\rm Ti}_4}{{\rm O}_{15}}$. Employing experimental design optimization theory, regression equations were established to correlate the ${{\rm Er}^{3 +}}/{{\rm Yb}^{3 +}}$ doping concentrations with the luminescent intensities. The genetic algorithm was utilized to compute the optimal solutions of the equations, resulting in ${{\rm Er}^{3 +}}$ and ${{\rm Yb}^{3 +}}$ doping concentrations of 3 mol% (molar fraction) and 20 mol% under 980 nm laser excitation and 3 mol% and 29.79 mol% under 1550 nm laser excitation. The up-conversion fluorescence emission spectra of the samples were measured under 980 nm excitation, revealing intense green emissions at 525 nm, 550 nm, and 662 nm, corresponding to the $^2{{\rm H}_{11/2}}{\to}{^4}{{\rm I}_{15/2}}$, ${^4}{{\rm S}_{3/2}}{\to}{^4}{{\rm I}_{15/2}}$, and $^4{{\rm F}_{9/2}}{\to}{^4}{{\rm I}_{15/2}}$ energy levels, respectively. Under 1550 nm excitation, peaks corresponding to the same energy levels were observed at 523 nm, 548 nm, and 661 nm. The relationship between the up-conversion fluorescence and the laser operating current for the optimal samples under 980 nm and 1550 nm excitations was explored, indicating two-photon and three-photon processes, respectively. Detailed analysis and discussion of the up-conversion fluorescence mechanisms were conducted. Additionally, the relationship between the up-conversion fluorescence and the temperature for the optimal samples was investigated, revealing excellent temperature sensing characteristics under 980 and 1550 nm laser excitations. The CIE coordinates for the optimal samples were calculated as (0.3111, 0.6747) and (0.5254, 0.4671) under 980 nm and 1550 nm excitations, respectively.
研究领域:
材料科学
·
光学
✓ 已验证
DOI
高影响因子
Future Medicinal Chemistry
·
2025
·
IF 3.4
Yan Li,Yajing Xing,Dongxia Huang,Jiuqing Xie,Yangrui Peng,Zhengfang Yi,Yihua Chen
Aim To develop potent B cell lymphoma 6 (BCL6) inhibitors, novel N-phenyl-4-pyrimidine-diamine analogs were designed and synthesized via structure-based and computer-aided drug design.Methods Starting from the hit compound ZB979, we synthesized three series of pyrimidinediamine BCL6 inhibitors (13a-13e, 14a-14c and 15a-15g) and evaluated their inhibitory activities on BCL6-SMRT interaction using homogeneous time-resolved fluorescence (HTRF) assays. The most promising candidate, compound 15d, was further assessed for its anti-proliferative activity and modulation of BCL6 downstream target genes, suppression of germinal center (GC) formation.Results Compound 15d demonstrated significant BCL6-SMRT inhibition with favorable physicochemical properties (Calculated LogP (ClogP) and topological polar surface area (tPSA)).Conclusion These findings highlight the potential of pyrimidinediamine-based scaffolds as novel BCL6 inhibitors, warranting further structural optimization to improve their efficacy.
✓ 已验证
DOI
PubMed
高影响因子
Synthetic and Systems Biotechnology
·
2025
·
IF 4.4
Xinru Wang,Lian Wang,Qihang Chen,Ke Wang,Huijing Wang,Dong Li,Song Gao,Weizhu Zeng,Jingwen Zhou
Hydroxysalidroside is an important natural phenylethanoid glycoside with broad application prospects in the food and pharmaceutical fields. However, its low concentration in plants and complex extraction hinder its production. Despite being a promising way to synthesize hydroxysalidroside in Escherichia coli , glycosylation remains the limiting factor for its production. A de novo biosynthetic pathway for hydroxysalidroside was successfully constructed in E. coli via the screening of glycosyltransferase, overexpressing phosphoglucomutase ( pgm ) and UDP-glucose pyrophosphorylase ( galU ) to ensure a sufficient supply of UDP-glucose (UDPG). Additionally, a semi-rational design of UGT85A1 was conducted to expand the acceptor-binding pocket to eliminate steric hindrance interfering with the binding of hydroxytyrosol. The endogenous genes ushA and otsA were knocked out to further reduce the consumption of UDPG. Finally, a titer of 5837.2 mg/L was achieved in a 5 L fermenter by optimizing the feeding times of carbon sources. This laid the foundation for the subsequent biosynthesis of phenylethanoid glycosides.
研究领域:
生物化学
·
合成生物学
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产品关联
DOI
PubMed
高影响因子
AMINO ACIDS
·
2020
·Vol. 52
·期 5
·831-839
·
IF 3.063
Zhao Dongxin,Lu Kui,Liu Guangbin,Zhu Hanjing,Ma Li,Qi Jianzhao,Yuan Libo
BRCA2 is an important tumor suppressor gene that plays a critical role in preserving the stability of cellular genetic information, participating in DNA repair by engaging in binding interactions with RAD51 proteins. However, the lack of structural data on BRCA2 and RAD51 makes the study of their interaction mechanism still a great challenge. We characterize the structure of the BRC8–RAD51 complex using ZDOCK protein docking software and identify the potential non-conserved active site of BRC8 via virtual alanine scanning, utilizing the obtained results to synthesize BRC8, its six analogous peptides (BRC8-1 to BRC8-6), and critical peptide fragment of RAD51 (RAD51(231–260)) by Fmoc solid-phase synthesis. The analogous peptides are found to exhibit a secondary structure significantly different from that of BRC8 by circular dichroism spectroscopy, which indicates that mutation sites determined by computer-aided simulation correspond to key amino acid residues substantially affecting polypeptide structure. On the other hand, the secondary structure of RAD51(231–260) was also considerably influenced by its interaction with BRC8 and analogs, e.g., the fraction of the α-helical structure in RAD51(231–260) increased to 23.6, 15.1, and 13.5% upon interaction with BRC8-1, BRC8-3, and BRC8-6, respectively. The results show that the properties of C-terminal amino acid residues significantly influence peptide–peptide interactions, in agreement with the results of virtual alanine scanning. Therefore, computer-aided simulation was confirmed to be a technique that is useful for narrowing down the range of sites responsible for interactions between peptides or proteins, and provides new inspirations for the design of peptides with strong interactions.
研究领域:
生物化学
·
分子生物学
·
结构生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MICROENCAPSULATION
·
2015
·
IF 1.585
Sha-Sha Li,Guo-Feng Li,Li Liu,Hui Li,Xiao Jiang,Xue-Ling Li,Zhi-Gang Liu,Ting Zuo,Li-Dong Weng,Qiang Liu
In this study, a central composite rotatable design based on response surface methodology (RSM) was employed to design and formulate an appropriate paeonol microparticle formulation. Five levels of a three-factor, rotatable, central composite design were used to evaluate the critical formulation variables. The optimum conditions for preparing paeonol-loaded microparticles were predicted to be: polyvinyl alcohol (PVA) content (2.84%), the ratio of drug to polymer (6.88) and the stirring rate (1007.59 rpm). The optimized responses for production yield and loading efficiency were found to be 68.86% and 55.90%, respectively, and the particle size were 23.27 ± 0.76 µm and the sorting coefficient (σ) was 0.732. Furthermore, in vitro release study suggested that microparticle could be a suitable delivery system in treating skin disease for its sustained release of drug. In conclusion, RSM can be successfully used to optimize the effect of formulation variables.
研究领域:
药剂学
·
药物递送系统
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产品关联
DOI
PubMed
高影响因子
BIOMACROMOLECULES
·
2025
·
IF 5.4
Yujia Lu,Guanyi Li,Yanwen Zhang,Yuxuan Ge,Bin Hao,Yu Yin,Yaxue Zhao,Yin Wang
Antimicrobial peptides (AMPs) are a class of peptides consisting of cationic amino acid residues and a hydrophobic segment, which have been used as an alternative to antibiotics in treating multidrug-resistant bacteria. However, the relationship among the molecular design, assembled structures, and resultant efficacy remains elusive. Herein, we report a class of constitutionally isomeric AMPs assembled into filaments with similar dimensions. Spectroscopic characterizations demonstrated that subtle changes in the position of amino acids led to dramatic variations in molecular packing and surface charges, which were verified by molecular dynamics simulations. In vitro antibacterial assays showed that all AMPs exerted antibacterial activity against Gram-positive methicillin-resistant Staphylococcus aureus (MRSA), but the efficacy was dependent on the molecular design. Given the good biocompatibility to eukaryotic cells, these AMPs could be potentially used as antibacterial agents. We believe that this finding provides an avenue to tune the bioactivity of AMPs by rational molecular design.
研究领域:
生物化学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
Small Methods
·
2025
·
IF 9.1
Yidan Zhang,Zhiguang Guo
The insufficient density and discontinuity of solar energy of photothermal superhydrophobic flexible film seriously affect the practical application. Light energy harvesting and heat energy storage are effective ways to solve this problem. Inspired by the viscous temperature-regulating material within the inflorescence of Lobelia telekii and the arrangement of bracts on its surface, a flexible film for photoheat storage is proposed that integrated a three-order photoheat trap and one-order heat storage. The surface of the flexible film features microcone array with micro-grooves, modified carbon nanotubes (MCNTs), and layered structures on microcapsules, forming a three-level photoheat trap. The generated heat increases the surface temperature and is partially absorbed by the heat storage material inside the microcapsule . The stable photothermal temperature of polyurethane films with microcone structure (S x ) was elevated by 3–5 °C compared to without it (Flat-S x ), while the stable photothermal temperature of MCNTs-S x (Flat-S x with MCNTs) exceeds that of S x by 2–6 °C. The ice particles on the MCNTs-S 0.45 completely dissolved within 180 s under xenon lamp light source. Meanwhile, MCNTs-S x demonstrated superhydrophobicity and outstanding anti-fouling capabilities. The fabricated MCNTs-S x realized biomimicry of Lobelia telekii in both structure and performance, providing a strategy for biomimetic photothermal de-icing.
研究领域:
材料科学
·
能源科学
·
仿生学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）