Foods
·
2023
·Vol. 12
·期 2
·293
·
IF 5.2
Pengbo Wang,Dongman Wan,Ting Peng,Yujing Yang,Xuefang Wen,Xianghui Yan,Jiaheng Xia,Qingwen Zhu,Ping Yu,Deming Gong,Zheling Zeng
Cinnamomum camphoraseed kernel oil (CCSKO) is one of the important natural medium chain triglycerides (MCT) resources, with more than 95.00% of medium chain fatty acids found in the world, and has various physiological effects. However, CCSKO has not been generally recognized as a safe oil or new food resource yet. The acute oral toxicity test and a standard battery of genotoxicity tests (mammalian erythrocyte micronucleus test, Ames test, and in vitro mammalian cell TK gene mutation test) of CCSKO as a new edible plant oil were used in the study. The results of the acute oral toxicity test showed that CCSKO was preliminary non-toxic, with an LD50value higher than 21.5 g/kg body weight. In the mammalian erythrocyte micronucleus test, there was no concentration-response relationship between the dose of CCSKO and micronucleus value in polychromatic erythrocytes compared to the negative control group. No genotoxicity was observed in the Ames test in the presence or absence of S9 at 5000 μg/mL. In vitro mammalian cell TK gene mutation test showed that CCSKO did not induce in vitro mammalian cell TK gene mutation in the presence or absence of S9 at 5000 μg/mL. These results indicated that CCSKO is a non-toxic natural medium-chain oil.Keywords:Cinnamomum camphoraseed kernel oil;acute oral toxicity;genotoxicity assessment;medium chain oil;safety assessment
研究领域:
毒理学
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食品科学
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药理学
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2018
·
IF 6.735
Han-Qing Zhao,Qi Liu,Yi-Xuan Wang,Zhao-Yang Han,Zu-Gao Chen,Yang Mu
Biochar as a multi-functional material has been widely used in the fields of catalysis and pollutant removal, but its feasibility to enhance biologic nitrobenzene reduction with the mixed culture and the long-term impact on nitrobenzene removal and microbial structure are lack of a clear clarification. This study aimed at evaluating the feasibility of biochar to enhance anaerobic reduction of nitrobenzene with a mixed culture. We initially investigated the effect of the biochar addition on nitrobenzene removal in short-term experiments and then possible enhancement mechanisms for the biochar were explored. Additionally, the impacts of different key parameters on biological nitrobenzene reduction were studied in the presence of biochar. Moreover, the long-term influence of the biochar on biological nitrobenzene removal with the mixed culture was also evaluated. The results showed that the estimated pseudo first-order rate constant of nitrobenzene removal in the presence of 1.0 g L −1 biochar was four times higher than that without biochar addition. The possible enhancement mechanisms may be owing to that biochar could activate the nitrobenzene molecules and accelerate the electron transfer between microbe and nitrobenzene. Additionally, the stimulating level of the biochar on biological nitrobenzene removal remarkably varied under different conditions. Long-term experiments demonstrated that biochar could not only increase nitrobenzene removal rate but also improve the system stability, which may be related to the shift of microbial communities that the relative abundance of nitrobenzene reduction related bacteria as well as electrochemically active bacteria was enriched in biochar addition systems.
研究领域:
环境科学
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生物技术
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微生物学
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DOI
高影响因子
Annals of Laboratory Medicine
·
2022
·Vol. 42
·期 5
·575-584
·
IF 4.941
Yuhang Deng,Qingxiang Liu,Zhenni Liu,Haijian Zhao,Weiyan Zhou,Chuanbao Zhang
Background To identify candidate external quality assessment (EQA) materials for normetanephrine and metanephrine measurements, we assessed the commutability of eight processed human plasma samples. The agreement between routine assays and the candidate reference measurement procedure (cRMP) was also evaluated. Methods Fifty-three clinical samples and eight processed plasma samples were prepared. The processed samples included pooled and individual plasma samples spiked with pure normetanephrine and metanephrine and non-spiked pooled and individual plasma samples. The clinical and processed samples were subjected to four routine isotope dilution tandem mass spectrometry assays and cRMP. Commutability was assessed based on two approaches recommended by the CLSI and International Federation of Clinical Chemistry (IFCC). Passing–Bablok regression and Bland–Altman analysis were used to evaluate the agreement between the routine assays and cRMP. Results The commutability results of the CLSI approach were better than those of the IFCC approach. For the CLSI approach, spiked individual plasma samples and spiked high-concentration pooled plasma samples were commutable for all routine assays for both analytes. The non-spiked pooled plasma sample was commutable for two out of four routine assays for metanephrine and three out of four routine assays for normetanephrine. The agreement between the routine assays and the cRMP was satisfactory, except for one routine assay showing significant bias. Conclusions High-concentration spiked pooled plasma samples and spiked individual plasma samples are candidate EQA materials for normetanephrine and metanephrine measurements.
研究领域:
临床检验学
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医学实验室科学
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DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2025
·
IF 6.1
Yubing Dai,Jiaxin Liu,Naibing Liang,Zhangpeng Guo,Jing Jin,Congjie Bi,Haijun Zhang,Ningbo Geng,Jiping Chen
Plasma and blood cells may exist different phase partitioning of elements due to their distinct properties and functions, while the distribution patterns of other elements across plasma, platelets, leukocytes, and erythrocytes remain largely unexplored. This study developed a method to rapidly separate these fractions of human blood and quantify 22 elements in each fraction. Notably, elements like Fe, Zn, and Rb were higher in erythrocytes, whereas Cd, Cr, V, and Sr were concentrated in leukocytes. Most metals detected in plasma account for < 40 % of the total concentration in blood, particularly toxic metals, with Cr, As, Cd, Hg, and Pb accounting for 63–75 % in blood cells, suggesting plasma-based assessments may underestimate actual exposure. Metal concentrations in plasma were positively correlated with those in whole blood ( ρ ≥ 0.48), except for Fe ( ρ = 0.13). We derived empirical ratios to estimate the internal exposure levels of elements from plasma to blood. Finally, we prioritized the assessment of potential acute and non-cancer risks associated with five toxic metals. As evidenced by PrHQ values, the toxicity level of As were the highest. Notably, the PrHQ values for these toxic metals in whole blood were approximately three times higher than those detected in plasma.
研究领域:
环境毒理学
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分析化学
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金属组学
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公共卫生
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环境科学
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产品关联
DOI
PubMed
高影响因子
Soil Ecology Letters
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2024
·Vol. 6
·期 4
·1-8
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IF 4.5
Liang Fang,Li Zhu,Wang Zinan,Ke Xin,Wu Longhua,Christie Peter,Luo Yongming
Egg hatching of the soil collembolan Folsomia candida and the effects of per- and polyfluoroalkyl substances were investigated.
New and effective laboratory methods for egg hatching studies with soil collembolans were established.
Per- and polyfluoroalkyl compounds (PFASs) have been used industrially worldwide and are persistent organic pollutants in many soils. Twenty eggs laid by synchronized adults of the collembolan Folsomia candida were added to each Petri dish containing compressed soil substrate mixed with perfluorooctanoic acid (PFOA), heptafluorobutyric acid (PFBA), or 6:2 chlorinated polyfluoroalkyl ether sulfonic acid (F-53B), and after 25 d of exposure the number hatched declined on average by 6.9%–49.7%, 10.3%–24.1%, and 3.4%–18.6%, respectively. PFASs delayed the peak of hatching by one day, and at different concentrations reduced the number of eggs hatched during the peak by 16.7%–30% and 23.3%–43.2% in PFOA and PFBA treatments, respectively. In the presence of F-53B the number of eggs hatched declined by 73.3% but the number of individuals increased by 29.3% at higher concentrations. The characteristics of egg hatching were stable and sensitive to PFASs, and may be suitable for use as indicators in the screening of contaminated soils for environmental risk assessment.
研究领域:
生态毒理学
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环境科学
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产品关联
DOI
高影响因子
FOOD SCIENCE AND BIOTECHNOLOGY
·
2016
·Vol. 25
·期 2
·631-636
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IF 0.699
Huang Xiaojun,Nie Shaoping,Yang Meiyan,Xie Jianhua,Li Chang,Xie Mingyong
Nine different edible oil types from 7 cities in China were analyzed. Trans fatty acid (TFA) contents in edible oils were determined, TFA distribution characteristics were analyzed, and a risk assessment was performed. Total TFA contents varied from 0.075±0.011 to 2.243±0.770 g/100 g of oil, with a mean content of 0.66 g/100 g of oil. TFA contents differed among different oil types and among different cities. Generally speaking, oil samples from northern areas of China contained more TFA, and lard usually had higher levels of TFA than other oils. An exposure assessment showed that the estimated daily intake of TFA from Chinese edible oils was low, and a risk assessment indicated that there was a low risk for TFA consumption in Chinese edible oils.
研究领域:
营养学
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食品安全
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公共卫生
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Aiping Li,Chen Wang,Yunfeng Chai,Zhiming Huang,Binbin Yao,Chengyin Lu,Miao Yu,Hongping Chen
Validamycin A (VA) and spinosyn A (SA) have been utilized as antibiotic pesticides in tea plantations for the control of pests and diseases. Both pesticides pose significant risks to human health and have been listed as key controlled new pollutants in China. So far, there have been few reports for the risk assessment of antibiotic pesticides in tea. In this study, a hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry (HILIC-MS/MS) method was developed for the simultaneous detection of VA and SA residues in tea. Method validation demonstrated good accuracy and precision. During tea planting, SA and VA in tea shoots degraded fast, with the half-life of 0.36 to 0.43 days for SA and 0.72 to 0.92 days for VA. SA and VA in tea shoots degraded fast initially and then followed by a slower phase. Both SA and VA degraded rapidly during the withering and drying stages. Compared to green tea, the degradation rates of both pesticides were faster during black tea manufacturing. Transfer rates of VA and SA from tea into infusions had transfer rates of 76.7 % to 99.5 % and 1.5 % to 2.7 %, respectively. The chronic risk quotient (RQc) < 1 for both antibiotic pesticides indicated negligible consumer risk from tea.
研究领域:
农业科学
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食品科学
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环境科学
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DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Jianting Ning,Jialu Chen,Wanting Liu,Xinping Chen,Xue Luo,Xiqing Yue
Amino acids serve as the fundamental building blocks of proteins which play crucial roles in various biological processes. However, there is a relative scarcity of comprehensive analysis regarding the variation of amino acids in donkey milk during different lactation periods and within different domains. Hence, we thoroughly examine the composition of both free and insoluble-proteome amino acids in donkey colostrum (DC) and donkey mature milk (DM). Our investigation includes an assessment of their nutritional significance, flavor analysis, metabolic pathways, and interactions across various lactation stages . Through nutritional assessment , we found that DC and DM exhibit excellent nutritional value . Both kinds of milk possess a delightful taste profile, with the majority of flavors concentrated in the realm of “savory umami” and “sweet-savory”. The most significant pathway of differentially expressed amino acids for both free and insoluble-proteome amino acids groups was “valine, leucine , and isoleucine biosynthesis”. These findings deepen our comprehension of the varying amino acid compositions within donkey milk across different lactation stages, paving the way for future research directions in developing functional foods utilizing donkey milk as a source.
研究领域:
营养学
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生物化学
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产品关联
DOI
高影响因子
AQUATIC TOXICOLOGY
·
2023
·
IF 4.5
Ferry Saputra,Michael Edbert Suryanto,Gilbert Audira,Cao Thang Luong,Chih-Hsin Hung,Marri Jmelou Roldan,Ross D. Vasquez,Chung-Der Hsiao
Daphnia magna is one species of water flea that has been used for a long time for ecotoxicity studies. In addition, Daphnia has a myogenic heart that is very useful for cardiotoxicity studies. Previous attempts to calculate the cardiac parameter endpoints in Daphnia suffer from the drawback of tedious operation and high variation due to manual counting errors. Even the previous method that utilized deep learning to help the process suffer from either overestimation of parameters or the need for specialized equipment to perform the analysis. In this study, we utilized DeepLabCut software previously used for animal pose tracking and demonstrated that ResNet_152 was the best fit for training the network. The trained network also showed comparable results with ImageJ and Kymograph, which was mostly done manually. In addition to that, several macro scripts in either Excel or Python format were developed to help summarize the data for faster analysis. The trained network was then challenged to analyze the potential cardiotoxicity of imidacloprid and pendimethalin in D. magna , and it showed that both pesticides cause alteration in their cardiac performance. Overall, this method provides a simple and automatic method to analyze the cardiac performance of Daphnia by utilizing DeepLabCut . The method proposed in this paper can contribute greatly to scientists conducting fast and accurate cardiotoxicity measurements when using Daphnia as a model.
研究领域:
生态毒理学
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计算生物学
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心血管研究
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES
·
2023
·
IF 2
Qingshan Feng,Lei Han,Qiong Wu,Xiaomao Wu
Rosa roxburghii is a medicinal and edible plant, which is favored by consumers due to its rich vitamin C content. Residues and potential health risks of difenoconazole in the R. roxburghii ecosystem has aroused a concern considering its extensive use for controlling the powdery mildew of R. roxburghii. In this study, the residue of difenoconazole in R. roxburghii and soil was extracted by acetonitrile, purified by primary secondary amine and detected by liquid chromatography-tandem triple quadrupole mass spectrometry. The average recoveries in R. roxburghii and soil matrix varied from 82.59% to 99.63%, with relative standard deviations (RSD) of 1.14%–8.23%. The limit of quantification (LOQ) and detection (LOD) of difenoconazole in R. roxburghii and soil samples were 0.01 mg/kg. The dissipation of difenoconazole followed well the first-order kinetic, with a half-life of 3.99–5.57 d in R. roxburghii and 4.94–6.23 d in soil, respectively. And the terminal residues were <0.01–2.181 mg/kg and 0.014–2.406 mg/kg, respectively. The chronic and acute risk quotient values of difenoconazole were respectively 0.42% and 4.1%, which suggests that the risk was acceptable and safe to consumers. This study provides a reference for the safe and reasonable use of difenoconazole in R. roxburghii production.
研究领域:
植物保护
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食品安全
·
分析化学
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产品关联
DOI
PubMed
高影响因子
Bioactive Materials
·
2022
·
IF 16.874
Yi Xiao,Haomin Yi,Jingzhi Zhu,Suhua Chen,Guofang Wang,Yilong Liao,Yuanyuan Lei,Liyin Chen,Xingcai Zhang,Fangfu Ye
Toxicity assessment is a major problem in pharmaceutical candidates and industry chemicals development. However, due to the lack of practical analytical methods for DNA adduct analysis, the safety evaluation of drug and industry chemicals was severely limited. Here, we develop a DNAzyme-based method to detect DNA adduct damage for toxicity assessment of drugs and chemicals. Among 18 structural variants of G4 DNAzyme, EA2 DNAzyme exhibits an obvious DNA damaging effect of styrene oxide (SO) due to its unstable structure. The covalent binding of SO to DNAzyme disrupts the Hoogsteen hydrogen bonding sites of G-plane guanines and affects the formation of the G4 quadruplex. DNA damage chemicals reduce the peroxidase activity of the G4 DNAzyme to monitor the DNA adduct damage by disrupting the structural integrity of the G4 DNAzyme. Our method for genotoxic assessment of pharmaceutical candidates and industrial chemicals can elucidate the complex chemical pathways leading to toxicity, predict toxic effects of chemicals, and evaluate possible risks to human health.
研究领域:
分子生物学
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毒理学
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药学
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化学工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Xiaojiao Dai,Ziling Huang,Chuanyi Liu,Zhigang Li,Qingmin Chen,Yi Xu,Xiaomei Chen,Quansheng Chen,Jie Wei
Adenosine triphosphate (ATP) levels are recognized as a critical indicator of microbial content in food safety. The development of highly efficient ATP-sensing methods is essential for assessing food freshness. Herein, we propose a simple-yet-efficient hemin/G-quadruplex (G4) DNAzyme-based aptasensor for ATP detection and oyster freshness assessment. The anti-ATP aptamer subunits specifically bind to ATP, leading to self-assembly of the hemin/G4 DNAzyme. The newly produced DNAzyme then catalyzes the oxidation of ABTS 2- to produce the colored ABTS •− , providing an absorbance response for sensitive ATP analysis. The aptasensor facilitates high-performance and accurate determination of ATP with a low detection limit of 0.065 μM. Furthermore, this sensor enables reliable ATP analysis in oyster samples and evaluation of oyster freshness. In particular, a good linear correlation was established between microbial content and ATP levels. The proposed aptasensor for the assessment of freshness is characterized by its simplicity and efficiency, potentially offering a new tool to monitor food safety.
研究领域:
生物化学
·
食品安全
·
分子生物学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）