BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
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2019
·Vol. 67
·期 3
·343-353
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IF 1.559
Hai-Feng Hu,Hai-Yan Zhou,Gao-Ping Cheng,Ya-Ping Xue,Yuan-Shan Wang,Yu-Guo Zheng
R -2-(4-hydroxyphenoxy)propionicacid (HPOPA) is a valuable intermediate for the synthesis of enantiomerically pure aryloxyphenoxypropionic acid herbicides. In this work, to improve the HPOPA biosynthesis by Beauveria bassiana ZJB16002 from the substrate R -2-phenoxypropionic acid (POPA), the original HPOPA producer B. bassiana ZJB16002 was subjected to physical mutagenesis with 137 Cs-γ irradiation and chemical mutagen N -methyl- N' -nitro- N -nitrasoguanidine (NTG) induced mutagenesis. The effects of different treatment doses of the mutagens on the lethal rate and positive mutation rate were investigated, and the results showed that the optimal 137 Cs-γirradiation dose and NTG concentration was 850 Gy and 500 µg/mL, respectively. Under these conditions, a mutant strain CCN-7 with the highest HPOPA production capacity was obtained through two rounds of 137 Cs-γ irradiation treatment followed by one round of NTG mutagenesis. At the substrate (POPA) concentration of 50 g/L, HPOPA titer of CCN-7 reached 36.88 g/L, which was 9.73-fold higher than the parental strain. The morphology of the wild-type and mutant strain was compared and the results might provide helpful information in exploration of the correlation of morphology and biochemical features of B. bassiana .
研究领域:
微生物学
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生物工程
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产品关联
DOI
PubMed
高影响因子
BREEDING SCIENCE
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2018
·
IF 1.559
Hiroyasu Yamaguchi
Ornamental plants that have a rich variety of flower colors and shapes are highly prized in the commercial flower market, and therefore, mutant cultivars that produce different types of flowers while retaining their growth habits are in demand. Furthermore, mutation breeding is well suited for ornamental plants because many species can be easily vegetatively propagated, facilitating the production of spontaneous and induced mutants. The use of ion beams in mutation breeding has rapidly expanded since the 1990s in Japan, with the prospect that more ion beam-specific mutants will be generated. There are currently four irradiation facilities in Japan that provide ion beam irradiation for plant materials. The development of mutant cultivars using ion beams has been attempted on many ornamental plants thus far, and some species have been used to investigate the process of mutagenesis. In addition, progress is being made in clarifying the genetic mechanism for expressing important traits, which will probably result in the development of more efficient mutation breeding methods for ornamental plants. This review not only provides examples of successful mutation breeding results using ion beams, but it also describes research on mutagenesis and compares results of ion beam and gamma ray breeding using ornamental plants.
研究领域:
植物学
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遗传学
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园艺学
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DOI
PubMed
高影响因子
FOOD SCIENCE AND BIOTECHNOLOGY
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2025
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IF 3.1
Nho Soo Bin,Do Sang-Hun,Oh Seongjin,Park Yong-Cheol,Kim Sun-Ki
This study aimed to develop a non-genetically modified organism (non-GMO) Saccharomyces cerevisiae strain with enhanced glutathione production using UV-induced random mutagenesis. Among 30 wild-type strains, S. cerevisiae KACC 48331 was selected for its high glutathione production. A #14 mutant with significantly higher glutathione content was isolated via acrolein resistance screening. Optimal batch fermentation was established at 20 °C and pH 4.5 using a cost-effective medium of molasses and corn steep liquor (CSL). In fed-batch fermentation, the #14 mutant achieved 1.98 g/L of glutathione using molasses and CSL as carbon and nitrogen sources, comparable to yields from expensive complex media containing peptone and yeast extract. Notably, over 99% of the glutathione produced was in the reduced, biologically active form. These results demonstrate the feasibility of using non-GMO yeast and industrial by-products for sustainable and economical glutathione production.
研究领域:
生物技术
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微生物发酵
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工业微生物学
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代谢工程
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应用微生物学
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
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2025
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IF 5.9
Zhanyu Shen,Fanghua Mo,Lei Wang,Yu Su,Huijing Han,Kaiyue Zheng,Chunling Wang
The ARTP (atmospheric and room temperature plasma) mutation breeding approach was employed to enhance the salt tolerance and metabolic capability of Torulopsis versatilis . Targeted screening for salt tolerance identified the positive mutant strain T20-3, which exhibited improved performance in media 18 % and 20 % salinity. The selected strain's genetic stability, metabolic performance, and fermentation characteristics were further evaluated through successive passaging and mash fermentation experiments. The results demonstrated that T20-3 yeast could maintain stable propagation under high salt conditions and exhibited robust flavor production through enhanced glycolysis, amino acid metabolism, and fatty acid metabolism pathways. During the fermentation of T20-3, the content of alcohols and esters was elevated by 95.28 % and 201.83 %, with a significant increase in isoamyl acetate and phenylethanol. The combination of headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS) analysis and sensory evaluation demonstrated that increased levels of key flavor compounds, including phenylethanol, acetic acid, isoamyl acetate, 3-hydroxy-2-butanone, and 2,3,5-trimethylpyrazine, contributed to a more pronounced floral, fruity, and sauce-like flavor profile in T20-3 soy sauce. In conclusion, the findings indicated that T20-3 enhanced the flavor quality of fermented soy sauce and holds considerable potential for improving fruity, floral, umami, and aftertaste profiles.
研究领域:
食品科学
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微生物学
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代谢组学
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DOI
高影响因子
BIOPROCESS AND BIOSYSTEMS ENGINEERING
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2018
·Vol. 41
·期 5
·729-738
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IF 2.139
Tong Qian-Qian,Zhou Yue-Hui,Chen Xiang-Song,Wu Jin-Yong,Wei Ping,Yuan Li-Xia,Yao Jian-Ming
The production of virginiamycin (VGM) from Streptomyces virginiae was improved by genome shuffling and ribosome engineering companied with a high-throughput screening method integrating deep-well cultivation and the cylinder–plate detecting. First, a novel high-throughput method was developed to rapidly screen large numbers of VGM-producing mutants. Then, the starting population of genome shuffling was obtained through ultraviolet (UV) and microwave mutagenesis, and four mutants with higher productivity of VGM were selected for genome shuffling. Next, the parent protoplasts were inactivated by UV and heat when a fusant probability was about 98%. Streptomycin resistance was used as an evolutionary pressure to extend positive effects on VGM synthesis. Finally, after five rounds of genome shuffling, a genetically stable strain G5-103 was obtained and characterized to be able to yield 251 mg/L VGM, which was 3.1- and 11.6-fold higher than that of the mutant strain UV 1150 and the wild-type strain, respectively.
研究领域:
生物技术
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微生物学
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药学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Microbiology
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2022
·
IF 6.064
Shi Zhihui,He Xiaoxian,Zhang Hailiang,Guo Xuena,Cheng Yanfei,Liu Xuelian,Wang Zhaoyue,He Xiuping
Carotenoids are unsaturated compounds with terpene groups. Among them, astaxanthin has strong antioxidant properties. It is widely used in aquaculture, food, medicine, and cosmetics with a broad market prospect. Phaffia rhodozyma is an important microorganism that synthesizes astaxanthin, but its wild strains have low pigment content, long growth cycle, and low fermentation temperature. Therefore, it is important to research the genetic improvement of the physiological and biochemical properties of P. rhodozyma. In this study, the atmospheric and room temperature plasma mutagenesis technology was adopted, through the functional evolution of the carotenoid production performance; then, through the comparative analysis of the genomics and transcriptomics of the wild strain and evolved strain, the key factor GST1 gene that affects carotenoid synthesis was discovered.
研究领域:
微生物学
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遗传学
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生物化学
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DOI
PubMed
高影响因子
Bioresources and Bioprocessing
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2017
·Vol. 4
·期 1
·1-7
·
IF 0
Jiang Ying,Shang Yue-Peng,Li Hao,Zhang Chao,Pan Jiang,Bai Yun-Peng,Li Chun-Xiu,Xu Jian-He
To improve the fermentation production of transglutaminase (TGase) from Streptomyces mobaraensis for applications in the food industry, the atmospheric and room-temperature plasma (ARTP) mutagenesis was applied to breed S. mobaraensis mutants with increased TGase production.After eight rounds of iterative ARTP mutagenesis, four genetically stable mutants, Sm5-V1, Sm6-V13, Sm2-V10, and Sm7-V12, were identified, which showed increased TGase production by 27, 24, 24, and 19%, respectively. The best mutant Sm5-V1 exhibited a maximum TGase activity of 5.85 U/mL during flask fermentation. Compared to the wild-type strain, the transcription levels of the zymogen TGase genes in the mutants increased significantly as indicated by quantitative real-time PCR, while the gene nucleotide sequences of the mutants did not change at all. It was shown that the overexpression of TGase zymogen gene in the mutants contributes to the increase in TGase production.ARTP is a potentially efficient tool for microbial mutation breeding to bring some significant changes required for the industrial applications.
研究领域:
微生物学
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食品科学
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工业生物技术
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DOI
PubMed
CARBOHYDRATE POLYMERS
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2025
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IF 12.5
Bingqian Nie,Jianle Chen,Wenkang Wang,Tong Cao,Xingqian Ye,Shiguo Chen,Zhiqiang Hou
Ganoderma lucidum samples from space-mutated strains transported by China's “Shenzhou” recoverable satellites, along with traditionally cultivated counterparts, were selected for this study. Polysaccharides from Ganoderma lucidum were extracted using a two-step sequential method integrating hot water and alkaline extractions. Liquid chromatography-mass spectrometry (LC-MS) was employed to characterize polysaccharide structures (monosaccharide composition and glycosidic linkages) and profile terpenoids, assessing the impact of space mutation. Results showed a significant increase ( p < 0.05) in polysaccharide and terpenoid contents, with polysaccharide yield rising from 178.59 % to 265.38 %. Space-mutated polysaccharides exhibited higher glucose content, altered monosaccharide composition, lower molecular weight, and an enriched β-1,3-glucan structure. They also demonstrated enhanced anti-inflammatory activity, underscoring the functional benefits of space-induced modifications. This study presents the first comprehensive analysis of structural alterations in Ganoderma lucidum polysaccharides induced by space mutation. It provides valuable scientific insights into the space breeding of medicinal plants and suggests that space mutation technology could serve as a promising strategy for developing functional foods from Ganoderma lucidum .
研究领域:
植物学
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药理学
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食品科学
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产品关联
DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
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2018
·
IF 3.466
Chenzhong Fei,Rufeng She,Guiyu Li,Lifang Zhang,Wushun Fan,suhan Xia,Feiqun Xue
Tenvermectin (TVM) is a novel 16-membered macrocyclic lactone antibiotics, which contains component TVM A and TVM B. However there is not any report on safety and clinical efficacy of TVM for developing as a potential drug. In order to understand the part of safety and clinical efficacy of TVM, we conducted the acute toxicity test, the standard bacterial reverse mutation (Ames) test and the clinical deworming test. In the acute toxicity studies , TVM, TVM A and ivermectin (IVM) were administrated once by oral gavage to mice and rats. Results showed that the oral LD 50 values of TVM, TVM A and IVM in mice were 74.41, 106.95 and 53.06 mg/kg respectively. The oral LD 50 values of TVM and TVM A in rats were determined to be 164.22 and 749.34 mg/kg respectively. TVM and IVM are moderately toxic substances, meanwhile the TVM A belongs to low toxic compounds, implying that the acute toxicity is highly related to the length of side chain of TVM at position C25. In the Ames test , results showed that TVM did not induce mutagenicity in Salmonella typhimurium TA97a, TA98, TA100, TA102 and TA1535 with and without metabolic activation system, speculating that the mutagenicity is probably not related to the side chain at position C25 of 16-membered macrocyclic lactone antibiotics. In the efficacy trail of TVM against swine nematodes, growing pigs natural infection of Ascaris suum and Trichuris suis were treated with a single subcutaneous injection 0.3 mg/kg b.w.. Results showed that TVM and IVM had excellent effect in expelling Ascaris suum , and TVM had potential efficacy against Trichuris suis , however IVM had no effect on Trichuris suis . This study suggests that the side chain of TVM at position C25 may have important biological functions, which is one of the key sites of the studies on structure-activity relationship of 16-membered macrocyclic lactone compounds. TVM is a new compound exhibited some advantages worthy of developing.
研究领域:
药理学
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毒理学
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寄生虫学
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产品关联
DOI
PubMed
高影响因子
AUSTRALASIAN PLANT PATHOLOGY
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2022
·Vol. 51
·期 5
·535-541
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IF 1.4
AL-Kubati Asma Mohammed Saeed,Kang Baoshan,Abbas Aqleem,Kaseb Mohamed Omar,Gu Qinsheng
Cucumber green mottle mosaic virus (CGMMV) is a major threat to cucurbits-producing regions worldwide. Conventional breeding process takes much time to cross plants and to select a good variety. In contrast to conventional breeding, mutation breeding accelerates the process of cultivar development and increases variety within a crop species. Hence, the aim of this study was to develop bottle gourd lines that were resistant to CGMMV using Ethyl Methane Sulfonate (EMS) mutagenesis. By treating wild-type seeds (M0) with EMS, bottle gourd lines (M1) were produced. "M1" lines were self-pollinated; as a result, 303 "M2" families were obtained. From M2 families, a total of 2444 "M3" lines were obtained to screen resistance to CGMMV. The "M3" lines were then classified into 5 levels based on the development of symptoms 11–30 days post-inoculation, with very light symptoms 13.91%, light symptoms 34.0%, mild symptoms 33.71%, severe symptoms 4.62%, and death lines 4.62%, respectively. About 278 (11.38%) "M3" lines in glasshouse experiments showed no symptoms at 30 dpi. Using a double-antibody sandwich enzyme-linked immunosorbent assay at 60 dpi, 44 "M3" lines out of 278 were determined to be asymptomatic and negative for CGMMV. These lines were then transplanted in the field. Four of the 44 "M3" lines were asymptomatic in the field and tested negative for CGMMV using the DAS-ELISA. The new findings show that using EMS-induced mutations to create novel CGMMV highly resistant bottle gourd cultivars could be a viable option.
研究领域:
植物学
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遗传学
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病毒学
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DOI
高影响因子
Frontiers in Microbiology
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2023
·
IF 5.2
Yanjun Zhang,Xiao Zhang,Weiliang Qiu
Lecanicillium lecanii (Zimmermann) Zare & Gams is used as an effective biopesticide for the control of sap-sucking insect pests on agricultural crops. However, low fungicide tolerance limits its large-scale field application. To improve the propamocarb tolerance in L. lecanii , a composite mutagenesis system was established by using UV-light (U), N-Methyl-N′-nitro-N-nitrosoguanidine (NTG) (N) and N + ion-beam (I). The permutation type of three agents was a consecutive mutagenesis treatment (I/N/U) after an intermittent treatment (U + N + I). The “U” mutagenesis was performed at 254 nm for 60 s and at a distance of 45 cm under a 20 W germicidal lamp, the “N” mutagenesis was performed at a concentration of 1.0 mg/mL NTG for 60 min, and the “I” mutagenesis was performed by low energy N + ion-beam using a dose of 10 × 10 13 ions/cm 2 at 30 keV. This composite mutagenesis system was recorded as the “U + N + I + I/N/U,” and then the mutagenesis efficiency in improving propamocarb tolerance was assessed by analyzing changes of mutants in the propamocarb sensitivity, mitotic stability, mycelial growth speed on plates or in liquid, sporulation on plates or aphids, conidial germination, 50% lethal concentration (LC 50 ) and 50% lethal time (LT 50 ) to aphids, lipid constituent and cell membrane permeability and control against aphids in the presence or absence of propamocarb. Compared to the wild-type isolate with a 50% effective concentration (EC 50 ) value of 503.6 μg/mL propamocarb, the Ll-IC-UNI produced by the “U + N + I + I/N/U” had the highest EC 50 value of 3576.4 μg/mL and a tolerance ratio of 7.1. The mutant was mitotically stable in 20-passage cultivation and did not show any unfavorable changes in growth and virulence indicators. The mutant showed the highest ability to resist or avoid the damaging effects of propamocarb as reflected by the alternations of lipid constituents and membrane permeability. The interval time for applying fungal agent was significantly shortened in this mutant after spraying a field recommended dose of 550 μg/mL propamocarb. In conclude, the “U + N + I + I/N/U” composite mutagenesis mode was efficient and useful to improve the propamocarb-tolerance of L. lecanii and the obtained Ll-IC-UNI could have commercial potential for field application.
研究领域:
植物保护
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微生物学
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Yujia Zhou,Zhiming Li,Xinghui Xie,Xingtao Zhou,Jielun Hu,Shaoping Nie
Gellan is a microbial polysaccharide synthesized by Sphingomonas paucimobilis , and is widely utilized in the food and pharmaceutical industries, as well as in other fields. However, much remains unknown about the key genes involved in the gellan synthesis pathway. In this study, ultraviolet (UV) and atmospheric room temperature plasma (ARTP) were employed to induce mutations in S . paucimobilis ATCC31461 (Sp31461 NC), and ultimately resulting a high-yield mutant strain (Sp31461 UA) with a yield increase of 120 % from 3.6 g/L to 8.3 g/L. The growth curve, monosaccharide composition and molecular weight indicated that mutagenesis accelerated the growth of the strain, but did not affect gellan's basic physicochemical property. A combined analysis of transcriptomic and metabolomic demonstrated that the differentially expressed genes and metabolites resulting from mutations were primarily enriched in pathways related to energy metabolism, among which potential key genes such as pgi , clpB , ycaJ associated with gellan yield, were further verified by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and gene overexpression. Notably, the strain overexpression the pgi gene resulted in a 20 % increase in gellan yield compared to the Sp31461 UA strain, reaching 10.0 g/L. In summary, the energy metabolism pathway in S . paucimobilis is closely linked to gellan biosynthesis, and high-yield strains of gellan can be developed through mutagenesis breeding and the overexpression of key genes identified by transcriptomics and metabolomics. This research offers valuable scientific insights for the industrial production and application of gellan.
研究领域:
微生物学
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工业生物技术
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分子遗传学
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代谢工程
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生物化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）