JOURNAL OF FISH DISEASES
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2022
·Vol. 45
·期 11
·1599-1607
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IF 2.58
Xin-Yuan Jiang,Zong-Yi Ren,Nian-Kun Zhang,Ke-Chen Yang,Gao-Xue Wang,Hai-Feng Jiang
Nocardiosis caused by Nocardia seriolae is a major threat to the aquaculture industry. Given that prolonged therapy administration can lead to a growth of antibiotic resistant strains, new antibacterial agents and alternative strategies are urgently needed. In this study, 80 medicinal plants were selected for antibacterial screening to obtain potent bioactive compounds against N. seriolae infection. The methanolic extracts of Magnolia officinalis exhibited the strongest antibacterial activity against N. seriolae with the minimal inhibitory concentration (MIC) of 12.5 μg/ml. Honokiol and magnolol as the main bioactive components of M. officinalis showed higher activity with the MIC value of 3.12 and 6.25 μg/ml, respectively. Sequentially, the evaluation of antibacterial activity of honokiol in vivo showed that honokiol had good biosafety, and could significantly reduce the bacterial load of nocardia-infected largemouth bass ( p < .001). Furthermore, the survival rate of nocardia-infected fish fed with 100 mg/kg honokiol was obviously improved ( p < .05). Collectively, these results suggest that medicinal plants represent a promising reservoir for discovering active components against Nocardia , and honokiol has great potential to be developed as therapeutic agents to control nocardiosis in aquaculture.
研究领域:
水产养殖学
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药用植物学
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微生物学
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DOI
PubMed
高影响因子
MARINE BIOTECHNOLOGY
·
2025
·Vol. 27
·期 3
·1-9
·
IF 2.8
Li Jinling,Zhou Zhiyan,Feng Fangjian,Yuan Wei,Zhang Yawen,Wang Jiaxing,Zhang Bin,Jin Haixiao,He Shan,Ding Lijian
Epilepsy is a serious brain disease that urgently needs new drugs to treat it. In this study, three new α -pyrone derivatives, nocardipones A − C ( 1 − 3 ), were isolated from the sponge-associated actinomycete Nocardiopsis sp. NBUDK19 guided by anti-epileptic bioactivity using an in vivo zebrafish model. Compounds 1 − 3 were isolated and purified by silica gel column chromatography combined with semi-preparative HPLC. The structures of 1 − 3 were elucidated by UV, HRESIMS, NMR spectroscopic analysis, optical rotation, and computational chemical calculations. Our pharmacological study showed that compounds 1 − 3 exhibited notable reductions in seizure-like locomotor activity at 5 μg/mL. The qPCR analysis showed that compounds 1 − 3 significantly regulated the mRNA expression levels of c-fos and genes associated with GABAergic-glutamatergic signaling, including gria1b and gat1 . The molecular docking revealed that compounds 1 − 3 strongly binding to 4-aminobutyric acid transaminase, with binding energies of − 7.3, − 7.8, and − 7.3 kcal/mol, respectively. This work enriches bioactive drug discovery to treat epilepsy and illustrates an underlying pharmacological and molecular mechanism of these natural α -pyrones.
研究领域:
药理学
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海洋微生物学
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神经科学
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DOI
PubMed
高影响因子
MOLECULES
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2025
·Vol. 30
·期 18
·3669
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IF 4.6
Zirun Zhao,Rou Liu,Jiawen Yu,Yunlong Liu,Mingchun Li,Qilin Yu
Soil saline–alkali stress is a major problem faced by global agriculture, and there is an urgent need to develop efficient amelioration strategies. While both probiotics and plant stress-resistant molecules play critical roles in the alleviation of crop stress, their efficient retention in crop rhizosphere regions remains a great challenge. In this study, the nanocarrier ZIF-8@SPBP@betaine (ZSBet) was constructed by introduction of the synthesized polysaccharide-binding protein (SPBP) and the stress-resistant molecule betaine to the metal–organic framework ZIF-8. During co-incubation, the probiotic Novosphingobium capsulatum and ZSBet efficiently bound together to form ZSBet + Novo co-assemblies, i.e., the integrated protein-ZIF-8-probiotic complexes mediated by polysaccharide-receptor recognition, which exhibited strong root-binding abilities. Microbiome analysis revealed that ZSBet + Novo reduced the α-diversity of rhizosphere bacteria and increased the absolute abundance of biofilm formation-related bacteria, e.g., Novosphingobium , Sphingobium , and Lactococcus . During wheat cultivation in saline–alkali soil, ZSBet + Novo reduced soil pH by 0.63 units, decreased soil salt content by 0.11 g/kg, and increased soil nutrient levels. Furthermore, the co-assembly enhanced the wheat grain number by 145.05% and reduced root malondialdehyde and proline contents by 42.00% and 39.13%, respectively. This study provides a new strategy for improving crop resistance under saline–alkali stress in combination with nanotechnology and synthetic biology.
研究领域:
环境微生物学
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纳米生物技术
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植物生理学
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土壤修复
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合成生物学
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作物抗逆育种
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分子生态学
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产品关联
DOI
PubMed
高影响因子
AMB Express
·
2023
·Vol. 13
·期 1
·1-8
·
IF 3.7
shi Shi,Cheng Yutong,Wang Shuai,Zhang Xiangmei,Han Fubo,Li Xiaojing,Dong Huijun
Nonomuraea gerenzanensis ( N. gerenzanensis ) is known for its ability to biosynthesize A40926, the precursor of the glycopeptide antibiotic (GPA) Dalbavancin. However, challenges and uncertainties related to the genetic manipulation of the rare actinomycetes remain. In order to improve the conjugation transfer of N. gerenzanensis , the crucial factors affecting conjugal transfer were evaluated, including agar medium, mycelial state, donor-recipient ratio, magnesium ion concentration, and antibiotic coverage time firstly. Additionally, γ-butyrolactone (GBL) for quorum sensing (QS) and antibiotics targeting bacterial walls were applied to evaluate their effects on conjugation transfer. As a result, the optimal conditions of 5%TSB of liquid medium, 24 h of the period time, V0.1 of agar medium, 30 mM of magnesium ion, the ratio 10:1 of donor-to-recipient, and 27 h of the overlaying time of antibiotic were determined. Furthermore, the results showed that autoinducer GBL and GPA teicoplanin had a synergetic effect on the conjugation transfer of N. gerenzanensis at a working concentration of 60 µM and 0.5 µg mL −1 , respectively. The highest conjugation efficiency could reach about 1.3 depending on the optimal process conditions and the interference of QS and antibiotics.
研究领域:
微生物学
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分子生物学
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遗传学
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生物化学
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生物技术
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抗生素研究
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DOI
高影响因子
Environmental Technology & Innovation
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2021
·
IF 5.263
Zhong Lin,Weijian Zhang,Xiaoping Zhu,Weijian Wu,Yijie Chen,Gaoyang Li,Lei Ren,Shuwen Luo,Yucheng Xie,Yongxiang Huang,Xinzi Wang,Zhen Zhen,Dayi Zhang
Di-2-ethylhexylphthalate (DEHP) is a common plasticizer and widely detected in sediments, water and biota. For its strong reproductive toxicity, teratogenicity and poisonousness, many approaches are developed to remediate DEHP-contaminated soils. This study aimed to evaluate the roles of biochar in accelerating DEHP degradation, assess the impacts of biochar pyrolysis temperature on DEHP removal performance, and explore the underlying mechanisms. Biochar had a satisfactory performance in DEHP adsorption and followed a Freundlich isotherm, exhibiting proportional adsorption capabilities to pyrolysis temperature. Biochar amendment significantly accelerated soil DEHP degradation, and the degradation efficiency ranged from 61.4% to 82.2% and increased with biochar pyrolysis temperature. Biochar remarkably changed soil physiochemical properties by increasing soil pH, organic matter and humus. Soil bacterial community was altered with biochar amendment that the relative abundance of Nocardioides , Marinobacter , Rhodococcus and Gramella increased. Our findings suggested that biochar-assisted bioremediation has a huge potential in soil DEHP degradation and pyrolysis temperature is a critical factor affecting the performance. Our work characterize biochar at different pyrolysis temperatures and explored the underlying mechanisms of biochar-assisted DEHP bioremediation, offering both scientific and practical hints for DEHP bioremediation in coastal sediments.
研究领域:
环境科学
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土壤学
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微生物学
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污染控制
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生物修复
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产品关联
DOI
高影响因子
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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2023
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IF 3.1
Yibo Guo,Ke Shen,Xinshuai Zhang,Hua Huang
l -threonate is the metabolite of vitamin C, while d -erythronate is the metabolite of N -acetyl- d -glucosamine, the nutritional supplement for joint health. They are widely distributed in the environment and human biofluids. Nevertheless, the catabolisms of l -threonate and d -erythronate are sparsely reported. Here we explored the functional diversity of an acid sugar kinase family (Pfam families PF07005-PF17042), and discovered a novel 2-oxo-tetronate kinase. The conserved genome neighborhood of the 2-oxo-tetronate kinase encodes members of class-II fructose-bisphosphate aldolase family (F_bP_aldolase, PF01116) and a dehydrogenase family (PF03446-PF14833). Instructed by this analysis, we experimentally verified that these enzymes are capable of degrading l -threonate into dihydroxyacetone phosphate (DHAP) in Arthrobacter sp. ZBG10, Clostridium scindens ATCC 35704, and Pseudonocardia dioxanivorans ATCC 55486. Meanwhile, a convergent catabolic pathway for d -erythronate was characterized in P . dioxanivorans ATCC 55486. Moreover, the phylogenetic distribution analysis indicates that the biological range of the identified l -threonate and d -erythronate catabolic pathways appears to extend mostly to members of the Actinomycetota , Cyanobacteriota , Bacillota , Pseudomonadota , and Bacteroidota phyla.
研究领域:
微生物学
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生物化学
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分子生物学
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基因组学
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代谢
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DOI
PubMed
高影响因子
CHEMOSPHERE
·
2022
·
IF 8.943
Lianghe Lv,Lina Sun,Chunli Yuan,Yue Han,Zhaohui Huang
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous persistent organic pollutants in soil, which have carcinogenic, teratogenic and mutagenic hazards. The effects of rhamnolipid (RL), nano zero-valent iron (nZVI), and anthraquinone-2,6-disulfonic acid (AQDS) on the degradation of PAHs in soil were studied. It was found that the treatment of 5 mg·kg −1 RL + 1% nZVI +0.2 mmol·kg −1 AQDS had the highest degradation rate. The degradation rate of total PAHs and HMW-PAHs was 72.81% and 79.47% respectively after 90 days. High-throughput sequencing showed that in RL + nZVI + AQDS enhanced soil, Clostridium , Geobacter , Anaeromyxobacter and Sphingomonas were the dominant species for anaerobic degradation of PAHs. Rhodococcus , Nocardioides , and Microvirga are the dominant species for aerobic degradation of PAHs. The activities of methyltransferase, dehydrogenase and catechol 1,2-dioxygenase in the anaerobic-aerobic degradation process of PAHs were consistent with the degradation process of PAHs, indicating the role of these enzymes in the degradation of PAHs. RL, nZVI, and AQDS combined enhanced microbial anaerobic-aerobic degradation has great application potential in remediation of PAHs-contaminated soil.
研究领域:
环境科学
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土壤学
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生物修复
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微生物学
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产品关联
DOI
PubMed
高影响因子
Molecular Catalysis
·
2024
·
IF 3.9
Qiang Chen,Zhi-Pu Li,Xiao-Qiong Pei,Yan Liu,M. Qaiser Fatmi,Zhong-Liu Wu
Chiral epoxides are one of the most versatile building blocks in organic synthesis, but the asymmetric epoxidation of unfunctionalized terminal olefins remains a challenge. Na StyA is a styrene monooxygenase from Nocardia altamirensis that functions as an enantioselective epoxygenase for terminal olefins. To improve its robustness, we investigated over 50 mutants designed via evolutionary- and energy-based approaches, and successfully identified 18 beneficial mutations, which then led to a combinatorial mutant of TM18 with drastically enhanced thermostability and solvent tolerance while retaining excellent activity and enantioselectivity. Its turnover frequency and half-life at 50 °C were increased to 5- and 148-fold of the parental. In the epoxidation of terminal olefins, TM18 achieved high product yields of over 4-fold of the parental, and excellent enantiomeric excess of 99 % to >99 % ee. The analysis of trajectory data from molecular dynamics simulations further validated that the mutations in TM18 increased its local rigidity, as inferred by RMSF and PCA analyses and through novel salt bridges formation. Furthermore, TM18 displayed reduced residue cross-correlation motions. The combined effects of these mutations result in an overall improvement in the structural and dynamic thermostability of TM18.
研究领域:
生物技术
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酶工程
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分子生物学
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化学工程
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有机化学
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产品关联
DOI
高影响因子
ENVIRONMENTAL RESEARCH
·
2023
·
IF 8.3
Haiping Gu,Jie Yan,Yuhao Liu,Xuewei Yu,Yan Feng,Xuanyi Yang,Su Shiung Lam,Mu. Naushad,Cheng Li,Christian Sonne
Bioaugmentation helps to obtain a microbiome capable of remediating polycyclic aromatic hydrocarbons (PAHs). In this study, acclimation of microorganisms to soil supplemented with phenanthrene (PHE) led to enrichment with PAH-degraders, including those in Actinobacteriota and in the genera Streptomyces , Rhodococcus , Nocardioides , Sphingomonas , and Mycobacterium . Aqueous (28 °C, pH 6.5) and soil cultures inoculated with PHE-acclimated soil showed a high PHE (ca. 50 mg L −1 ) degradation efficiency. The PHE degradation kinetics in aqueous and soil incubations fitted to the Gompertz equation and the first-order kinetic equation, respectively. Indigenous microorganisms adapted to PHE in their environment, and this increased their capacity to degrade PHE. The effect of co-contaminants and pathway intermediates on PHE degradation showed that the degradation of PHE improved in the presence of diesel while being hindered by lubricant oil, catechol, salicylic and phthalic acid. Our findings provide theoretical and practical support for bioremediationof PAHs in the environment.
研究领域:
环境微生物学
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土壤科学
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生物修复技术
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产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2024
·
IF 7.4
Shuangshuang Li,Xin Jiang,Chi Zhao,Yuan Ren,Lixin Luo
Biosurfactants combined with bioaugmentation for the remediation of petroleum hydrocarbon-contaminated soils proved to be one of the most effective ways. However, research on lipopeptides combined with bioaugmentation is rare, and the biological mechanisms are undefined. Therefore, the effect of Raoultella planticola and the lipopeptides it produces to restore contaminated soils was investigated using n-hexadecane as a model of petroleum hydrocarbons. After 60 d, n-hexadecane removal rate increased from 31.3% in contaminated soil to 39.9% with R. planticola alone and 59.0% with R. planticola together with 1.0 g intracellular lipopeptide (INLS) and 7.5 g sodium gluconate (SG)/kg dry soil. Meanwhile, the nitrogen and phosphorus components of the soil increased by 13.9% and 8% in the experimental group, respectively. Besides, DHA activity was 6.7 times that of the control group at 28 d, indicating a considerable increase in DHA activity. Microbial community analysis revealed a considerable increase in functional genera of Sphingomonas , Nocardia , Pseudoalteromonas , Povalibacter , Apiotrichum , Pseudallecheria , and Ascodesmis . The amount of fungus in the experimental group was more than 30 times that of the control group after 60 d. Microbial co-occurrence networks showed improved interactions of Firmicutes and Ascomycota, encouraging synergistic cooperation among microbes and thereby accelerating n-hexadecane breakdown. At once, the metabolic pathways ko00071 and ko00980 for n-hexadecane degradation were greatly elevated, indicating that functional genes such as alkane 1-monooxygenase and NADPH-cytochrome P450 reductase were activated. These results implied that combining hydrocarbon-degrading microbial with INLS was a promising bioremediation technique to remove soil petroleum hydrocarbons.
研究领域:
环境科学
·
微生物学
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生物技术
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土壤学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）