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Innovative Food Science & Emerging Technologies · 2024 · IF 6.3

Modified gluten by extrusion can significantly improve the amounts of flavor compounds during the fermentation of umami condiment

Li-Hao Wang,Wen-Hui Qu,Hui Jiang,Song-Gang Xia,Xue Feng,Chang-Hu Xue,Yun-Qi Wen

Gluten is widely used in food process, but rarely used in the fermentation of umami condiment. The extrusion treatment could modify the microstructure of gluten, which may further influence fermentation effectiveness. This study applied gluten and modified gluten obtained by extrusion as raw materials to koji and moromi fermentations. During the koji fermentation, water and Aspergillus oryzae applied to modified gluten penetrated into the inner layer of raw material more efficiently when compared with their application to gluten. After the fermentation, the amino acid nitrogen contents of prepared four group samples ranged from 0.80 g/100 mL to 1.05 g/100 mL, while the contents of glutamic acid, adenosine monophosphate (AMP), guanosine monophosphate (GMP), acetic acid and lactic acid etc. had significant increases, indicating the utilization of gluten in fermentation could produce large amounts of flavor compounds. Additionally, the modification of gluten accelerated the fermentation of A. oryzae and improved the utilization and efficiency of fermentation raw materials, while the soybean protein isolate could partially replace gluten to produce high amounts of flavor compounds during the fermentation. This study broadened the utilization scope of gluten in food industry and provided a reference for the fermentation of umami condiment.

研究领域: 食品科学 · 微生物学
✓ 已验证 DOI 高影响因子
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FOOD CHEMISTRY · 2021 · IF 7.514

Single-emission dual-enzyme magnetosensor for multiplex immunofluorometric assay of adulterated colorants in chili seasoning

Tian Guan,Zhuo Jiang,Zaoqing Liang,Yingju Liu,Weijuan Huang,Xiangmei Li,Xing Shen,Mengting Li,Zhenlin Xu,Hongtao Lei

In this work, a single-emission, dual-enzyme immunofluorometric magnetosensor was fabricated to simultaneously detect three illegal colorants in chili seasoning. Specifically, two enzymatic reactions catalyzed by horse radish peroxidase-labeled Rhodamine (RhB) antibody and glucose oxidase-labeled Sudan dyes (SuDs) antibody were performed within a functional microfluidic chip, leading to production of strongly fluorescent Resorufin . In addition, a compact analyzer assisted by a smartphone was developed to quantify signals. Compared with the available multiplex optical biosensors, this work demonstrated four superiorities: 1) Simple optical structure. Only single wavelength excitation/emission module was needed; 2) High multiplexing capacity through spatial resolution and signal resolution; 3) Precise determination by discriminant analysis; 4) Easy-operated and high-throughput parallel detection on 16-channel chips. Ultralow detection limits for RhB (0.0072 ng/mL), Sudan I (0.0040 ng/mL) and Sudan II (0.0260 ng/mL) were obtained by this magnetosensor, which opens a new approach in field detection of multiplex illegal dyes in food system.

研究领域: 食品科学 · 生物传感技术 · 分析化学
✓ 已验证 DOI PubMed 高影响因子
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FOOD RESEARCH INTERNATIONAL · 2017 · IF 3.086

Characterization of taste and aroma compounds in Tianyou, a traditional fermented wheat flour condiment

Xianli Gao,Junke Zhang,Joe M. Regenstein,Yiyun Yin,Cunshan Zhou

Taste and aroma compounds in Tianyou were determined using HPLC and GC–MS/GC-olfactometry. By comparison with light soy sauce (control), the contents of salt, sugar, total nitrogen and total acid in Tianyou were higher, while the contents of umami, sweet and bitter free amino acids, and the percentage of 1–5   kDa peptides in Tianyou were lower. Thirty-one aroma-active compounds were identified in both Tianyou and the control (30 compounds in common). Aroma extraction dilution analysis indicated that most flavor dilution factors of aroma-active compounds were lower in Tianyou than the control. Quantitative descriptive analysis showed that Tianyou had significantly stronger salty and sweet tastes, weaker umami taste and weaker malty, caramel-like and smoky notes when compared to the control ( p   <   0.05), which were in agreement with the analyses of taste and aroma compounds. This confirms that Tianyou has a distinctively different flavor from light soy sauce.

研究领域: 食品科学 · 化学 · 风味分析
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FOOD CHEMISTRY · 2024 · IF 8.5

Characteristic volatile compounds contributed to aroma of braised pork and their precursor sources

Yishun Yao,Meigui Huang,Xiaomin Wang,Jingyang Yu,Heping Cui,Khizar Hayat,Xiaoming Zhang,Chi-Tang Ho

The characteristic aroma compounds of traditional braised pork were investigated by gas chromatography–mass spectrometry−olfactometry (GC–MS–O), odor-activity values, and aroma recombination and omission experiments. A total of 56 volatile compounds were detected by GC–MS, among which hexanal, octanal, nonanal, ( E )-2-octenal, 2,3-octanedione, 1-octen-3-ol, 2-pentylfuran, methanethiol, and dimethyl trisulfide were identified as the key aroma compounds by molecular sensory science. Partial least squares regression analysis indicated that some aroma compounds significantly contributed to fatty (hexanal, heptanal, 2-pentylfuran, nonanal, and ( E )-2-octenal), meaty (methanethiol, dimethyl disulfide, dimethyl trisulfide, and octanal), sauce-like flavor (3-hydroxy-2-butanone and 2-furfural), and sweet, caramel (2,3-octanedione, 1-octen-3-ol). Lean meat produced more aldehydes, alcohols, ketones, and sulfur-containing compounds than subcutaneous fat. The seasonings (saccharose, cooking wine, and soy sauce) facilitated the formation of ethyl L-lactate, 2-acetylfuran, 2-furfural, 5-methyl-2-furaldehyde, 2-methyl-pyrazine, and 2-acetylpyrrole. Meanwhile they reduced the content of lipid oxidation products, thereby stimulated the characteristic aroma of the Chinese traditional braised pork.

研究领域: 食品科学 · 分析化学 · 感官科学
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