FOOD AND AGRICULTURAL IMMUNOLOGY
·
2024
·
IF 1.7
Jiaxin Wang,Wenhua Shang,Shibei Shao,Jialin Zhang,Qiuyang Wu,Min He,Licai Ma,Jianzhong Shen,Zhanhui Wang,Kai Wen
Paxilline (PAX) is a tremorgenic mycotoxin that is thought to be associated with various syndromes in animals. To facilitate the determination of this toxin, a hapten was chemically synthesized and then respectively conjugated to Bovine Thyroglobulin (BTG) and Bovine serum albumin (BSA) for antibody production. Monoclonal antibody (mAb) 4F8 was finally obtained and used to establish an accurate and sensitive indirect competitive ELISA (icELISA). Various parameters that could impact the performance of icELISA were optimized, including the ion concentration, pH value and organic solvent. Under optimal condition, this assay exhibits an IC50 value as low as 0.84 µg L−1. The limit of detection (LOD) of the established icELISA method for PAX was 0.03–0.3 µg L−1. In conclusion, this study provided a rapid, sensitive, and high-throughput immunoassay for the detection of PAX in grains.
研究领域:
免疫学
·
食品科学
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Qingwen Huang,Wenbo Guo,Xiuying Zhao,Haojie Cao,Kai Fan,Jiajia Meng,Dongxia Nie,Yongjiang Wu,Zheng Han
Due to the challenge of hundreds of potential mycotoxins that may be present in cereals, a rapid and reliable ultra-high performance liquid chromatography coupled with quadrupole-time-of-flight tandem mass spectrometry (UHPLC-Q-TOF MS) technique was developed for universal screening of 200 mycotoxins (prototype, emerging and related derivatives) in cereals. With satisfactory sensitivity of accurate mass full-spectrum acquisition, it is feasible to preliminarily identify tentative untargeted mycotoxins with a large range of polarity without reference materials. The current screening method was also validated by the determination of 33 typical mycotoxins, and the screening detection limits in the range of 0.5–100 μg kg −1 were established in cereals. In total, 138 stored samples were contaminated by 46 mycotoxins and their metabolites, some of which were firstly reported in cereals, posing emerging potential health risks to humans and animals. Furthermore, the accumulation, transformation and degradation mechanisms of typical mycotoxins in cereals were investigated under real storage conditions.
研究领域:
食品安全
·
分析化学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Zuolong Yu,Beizhen Hu,Yao Chen,Chaoqun Huang,Chao Han,Yan Shen
A gas chromatography-tandem mass spectrometry (GC − MS/MS) method was established for accurately determining penicillic acid in cereals. The cereal samples were extracted using acidified acetonitrile, purified by solid-phase extraction, and derived using N,O-bis(trimethylsilyl) acetamide for GC − MS/MS analysis. Recovery experiments were conducted with low, medium, and high concentrations. The recovery rates were 72.1%–89.8%, the relative standard deviations were less than 8.6%, and the limit of quantification was 20 μg/kg. The established method was rapid, efficient, sensitive, and specific, meeting the requirements for the determination of penicillic acid in cereals. The method exhibits high sensitivity, accurate results, and stable recovery, making it suitable for detecting penicillic acid residues in cereals.
研究领域:
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Ying-yue Zhang,Mei-juan Zhao,Chun-yao Liu,Kai Ma,Tian-yu Liu,Fei Chen,Li-na Wu,De-jun Hu,Guang-ping Lv
Immunoassay-based techniques are important on-site screening tools for the detection of mycotoxins in cereals. This study aims to evaluate the trueness, precision, repeatability and cross-reactivity of commercially available test strips, ELISA kits and UHPLC-MS/MS on analyzing zearalenone, ochratoxin A, deoxynivalenol, T-2 toxin and fumonisin B 1 . The results showed that false negative rate (25.7 %–37.4 %) of all tested mycotoxins by test strips was higher than the false positive rate (0 %–31.0 %). The repeatability of ELISA kits at the declared LOD dispersed from −85.7 % to +98.4 %. ELISA kits were more accurate at 50 % of the maximum residue limit (MRL) of mycotoxins than 150 % and 200 %. All the tested deoxynivalenol/zearalenone derivatives had cross-reactivity with different level, and sample matrix could reinforce this overestimation of target mycotoxin. This study emphasized that higher-quality antibody screening and more analytical performance investigations are need to address for on-site detection of mycotoxins in the future.
研究领域:
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 62
·38884-38894
·
IF 3.108
Tianjiao Xie,Min Zhang,Pan Chen,Haitian Zhao,Xin Yang,Lei Yao,Hua Zhang,Aijun Dong,Jing Wang,Zhenyu Wang
In this study, we report a facile method for the preparation of molecularly imprinted polymer based graphene for the electrochemical detection of thiamethoxam residue. We choose p-vinylbenzoic acid as a functional monomer; it can be used to add recognition units and alkenyl units onto the surface of graphene via π–π interactions in just one step, effectively simplifying the reaction process. And an ultra-thin imprinting film (2 nm) is formed by reducing the addition step involving the alkenyl modifier. Due to the irreversible electrochemical reduction characteristics of thiamethoxam, the template can be removed easily using cyclic voltammetry scanning, without the need for organic solvents. The performance of the fabricated sensor was evaluated and the results indicated that the sensor exhibited excellent specific recognition abilities for thiamethoxam detection; the imprinting factor is 2.36. The peak current from thiamethoxam is linearly proportional to its concentration over the range from 0.5 to 20 μM, and the detection limit is 0.04 μM. The practical application of the sensor was also realized in the selective detection of thiamethoxam in real samples.
研究领域:
分析化学
·
环境科学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Haoran Luo,Zehua Zhang,Yiyuan Zou,Zhiling Tang,Miaomiao Gu,Yun Zhou,Guohua Zhao
Reconstituted noodles containing multi-grain are superior to plain noodles in terms of health benefits, but their lower gluten levels cause deterioration in cooking performance and textural quality. To this end, this study investigated the efficacy of gluten pre-hydration in a model dough. The results indicated that, with the increase in the ratio of pre-hydrated gluten, the final hydration level of gluten in reconstituted noodles, the proportion of ordered secondary structures of gluten, and the intensities of molecular interactions continuously increased, resulting in a more compact gluten network. This made the dough more deformable, granting noodles higher cooking resistance (optimal cooking time: 230 s to 265 s) and hardness (1837 g to 2538 g). Gluten pre-hydration was more effective, convenient, and cost-efficient than existing methods in improving the quality of reconstituted noodles. However, it lowered the yield and transparency of cooked noodles and made them yellowish.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD AND AGRICULTURAL IMMUNOLOGY
·
2018
·
IF 2.568
Zhi-Li Xiao,Wei Tian,Zhen-Lin Xu,Yuan-Ming Sun
In this study, we develop a sensitive indirect competitive chemiluminescent enzyme immunoassay (ic-CLEIA) to detect cadmium (Cd) in cereals. A luminol-hydrogen peroxide chemiluminescence system catalysed by horseradish peroxidase (HRP) was used as the signal detecting system. The method showed a half-maximal inhibition concentration (IC50) of 0.5 ng/mL and a limit of determination (LOD) of 0.06 ng/mL. The assay indicated an effective linear range from 0.16 to 1.56 ng/mL. There were no obvious cross-reactivities with other metal ions, including Hg2+, Pb2+, As2+, Ag+, Fe3+, Cu2+, Cr2+, Au3+, Ca2+, Mg2+, Mn2+ and Zn2+. Recovery rates ranged from 79.2% to 120.0% for rice, maize, wheat, brown rice and glutinous rice samples. Twenty-five practical cereal samples were analysed, and the comparative analysis of the results showed a strong correlation (r = 0.984) between the CLEIA and the GFAAS methods. The results indicated that the ic-CLEIA developed was acceptable to screen for cadmium in cereal samples.
研究领域:
食品科学
·
环境科学
·
免疫学
✓ 已验证
产品关联
DOI
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Xing Chen,Menglin Jiang,Jie Liu,Genyi Zhang
Whole grain food is a type of healthy food with many beneficial effects, but the fundamental mechanism is still illusive. In the current study, a whole grain component (WGC), as a whole grain model, was prepared by mimicking the endosperm cellular structure through encapsulating glucomannan (dietary fiber) and starch in a matrix formed by polysaccharide gelation. A high level of slowly digestible starch (SDS) (>50 %) in WGC was observed from an in vitro digestion experiment. When the in vivo test of WGC was conducted, a reduced postprandial glycemia and an increased GLP-1 response were observed, which confirmed the stimulation effect of WGC on the secretion GLP-1, an incretin hormone with numerous health benefits. Further study showed that the blood glucose level (from 29.2 to 9.5 mmol/L) and the biomarker HbA1c (from 10.5 to 6.4 %) in diabetic mice were significantly reduced under the condition of WGC-containing diet (WGC-diet). The β-cell function and insulin sensitivity were also significantly improved. Furthermore, the WGC-diet reversed the dysbiosis with increased production of healthy short-chain fatty acids, which are likely the basis to the secretion of GLP-1. Gene expression revealed that WGC-diet activated the GLP-1 signaling pathway, leading to an increased secretion of insulin and improved β-cell function, which all contribute to the glycemic control in diabetic mice. Thus, GLP-1, as pleotropic incretin hormone, could be an important mechanism to the various health benefits of whole grain foods.
研究领域:
营养学
·
分子生物学
·
医学
✓ 已验证
DOI
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Lu Zeng,Huan Lv,Xiangrui Li,Wenjia Yang,Wencan Luo,Linghong Kong,Qiujin Zhu
Cyclosulfamuron (CS) is a widely used sulfonylurea herbicide in plant cultivation and food production, and poses significant threats to ecological balance and food safety. To address this concern, we designed a novel CS hapten using computer-simulated semi-antigens. A monoclonal antibody (mAb) exhibiting high specificity and affinity was generated, with an affinity constant of 1.049×10 9 L/mol and a semi-inhibitory concentration (IC 50 ) of 4.94 ng/mL. Leveraging this anti-CS mAb, we constructed a rapid, visually detectable, quantitative lateral flow immunoassay (LFIA) to detect CS in cereals. Under the optimal conditions, the cut-off values were determined to be 100 μg/kg for brown rice and corn samples and 50 μg/kg for rice samples. The corresponding limits of detection values (LODs) were found to be 1.93 μg/kg in brown rice, 2.18 μg/kg in corn and 1.93 μg/kg in rice, respectively. The recovery rates ranged from 96.2% to 104.8% with coefficients of variation ranging from 3.5% to 6.4%, showing good agreement with the results obtained by liquid chromatography–tandem mass spectrometry (LC–MS/MS). The proposed assay demonstrated robust analytical performance, with significant application potential for rapid, on-site detection.
研究领域:
食品安全
·
分析化学
·
免疫学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Xiaofang Li,Maozhong Sun,Liguang Xu,Liqiang Liu,Xinxin Xu,Hua Kuang,Chuanlai Xu
Ochratoxins (OTs, including OTA, OTB, and OTC), exhibit nephrotoxicity, carcinogenicity, teratogenicity, and immunotoxicity, contaminating wheat, maize, and rice, so endangering human health. In this study, we designed and screened haptens using computational chemistry to prepare mAb 4G11, which simultaneously recognized OTA, OTB, and OTC with high sensitivity and specificity. We developed a fluorescent microsphere immunochromatographic assay (FMIA), which could detect the total amount of OTs with a visual detection limit of 0.5 ng/mL and a quantification limit of 0.27 ng/mL. Recovery experiments ranged from 95.94 % to 106.01 % with coefficients of variation ranging from 2.74 % to 6.30 %. The results obtained from the FMIA were found to be in alignment with those obtained from liquid chromatography-tandem mass spectrometry. The FMIA reliability was further demonstrated using real samples. FMIA can serve as a valuable instrument for the surveillance of the content of OTs in cereals.
研究领域:
食品安全
·
分析化学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Xu Jing,Jing Wu,Huihui Wang,Luyao Guo,Xiaojiao Zheng,Xiaowen Wang,Shuo Wang
A novel deep eutectic solvent-based extraction and sulfur-doped carbon quantum dots (S-CQDs) serving as fluorescence probes to detect pirimicarb in cereals were established. The deep eutectic solvent was synthesized using choline chloride and butanediol, achieving direct and efficient extraction of pirimicarb residue in the cereals. The fluorescence quenching of S-CQDs was caused by the electrostatic interaction between the negatively charged S-CQDs and positively charged thiocholine, which was the hydrolysate of acetylthiocholine. The fluorescence of S-CQDs was enhanced as the activity of acetylcholinesterase (AChE) was inhibited by pirimicarb, achieving the detection of pirimicarb in the cereal samples. The limit of detection (LOD) was 0.006 μg mL −1 . The recovery ranged from 96.6% to 108.2%. This extraction and detection method of pirimicarb based on an environmentally friendly DES and S-CQD fluorescent sensor maintains good stability and convenience, offering a promising strategy for extracting and testing harmful substances in food samples.
研究领域:
分析化学
·
食品安全检测
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2025
·Vol. 14
·期 6
·1040
·
IF 5.1
Yan Zeng,Jie Wang,Mengxiao Bao,Yue Wu,Zhigang Chen
Whole grain foods have been recommended to preserve biologically active components and benefit human health. The effect of the addition amount of whole sweet potato flour (WSPF, 25%, 51%, and 75%) on the physicochemical and structural properties of extruded whole-grain noodles was evaluated. Compared with traditional wheat flour (WF), the increased content of WSPF led to an enhancement in the dough’s water retention capacity, resulting in the reduction of dough development time and stability time. The modulus of elasticity and the modulus of loss of the dough exhibited a positive correlation with the proportion of WSPF added, while the tangent value and maximum creep flexibility were negatively correlated. Confocal laser scanning microscopy (CLSM) observed that WSPF induced protein aggregation in the dough. Compared to conventional WF, the increased incorporation of WSPF resulted in improved textural characteristics of the extruded noodles. Sensory evaluation indicated that the addition of WSPF could enhance the quality of the noodles by imparting a sweet potato aroma, a distinctive color, and a satisfactory taste. These characteristics were correlated with their enhanced relative crystallinity, enthalpy, and short-range ordered structure. Additionally, 75% whole-grain sweet potato noodles exhibited the highest relative crystallinity (11.05%), enthalpy of pasting (ΔH, 22.6 J/g), and short-range ordered structure (0.78). SEM results indicated that the presence of holes in the cross-section of the sweet potato extruded noodles facilitated their rapid rehydration. Overall, the whole-grain sweet potato noodles have great potential in promoting the textural, sensory, and nutritional properties compared to traditional wheat noodles.
研究领域:
食品科学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）