INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2021
·
IF 6.953
Lu Cao,Jian Zhu,Na Li
Selenium (Se) is an essential trace element for human beings and animals. Traditional plant Se enrichment technology suffers from selenium pollution. Herein, environmentally friendly Se-agarose (Se-Agar) hybrid hydrogels are prepared by simply mixing agar with different Se species including selenocarrageenan (SeCA), selenite and Se yeast under heating and stirring for 0.5 h without any other reagent. Such Se-Agar hybrid hydrogels with excellent biocompatibility were used as natural substrates for the cultivation of Se-enriched mung bean sprouts. Compared with Se yeast, SeCA and selenite show a better Se enrichment effect on mung bean sprouts. Furthermore, the growth indices including plant weight and plant height of mung bean sprouts were investigated with different concentrations and sources of Se. Notably, the Se-Agar hybrid hydrogels could be easily regenerated and reused for multiple cycles. The results indicated that Se-Agar hybrid hydrogels as recyclable natural substrates offer a simple, sustainable and affordable strategy for plant Se enrichment.
研究领域:
植物学
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环境科学
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食品科学
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DOI
高影响因子
Antibiotics-Basel
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2022
·Vol. 11
·期 6
·807
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IF 5.222
Jing Cao,Yajie Wang,Guanzhao Wang,Pingping Ren,Yongning Wu,Qinghua He
Antimicrobials may be used to inhibit the growth of micro-organisms in the cultivation of mung bean sprouts, but the effects on mung bean sprouts are unclear. In the present study, the growth performance, morphology, antimicrobial effect and antimicrobial residues of mung bean sprouts cultivated in typical antimicrobial solutions were investigated. A screening of antimicrobial residues in thick-bud and rootless mung bean sprouts from local markets showed that the positive ratios of chloramphenicol, enrofloxacin, and furazolidone were 2.78%, 22.22%, and 13.89%, respectively. The cultivating experiment indicated that the production of mung bean sprouts in antimicrobial groups was significantly reduced over 96 h (p< 0.05). The bud and root length of mung bean sprouts in enrofloxacin, olaquindox, doxycycline and furazolidone groups were significantly shortened (p< 0.05), which cultivated thick-bud and rootless mung bean sprouts similar to the 6-benzyl-adenine group. Furthermore, linear regression analysis showed average optical density of 450 nm in circulating water and average production had no obvious correlation in mung bean sprouts (p> 0.05). Antimicrobial residues were found in both mung bean sprouts and circulating water. These novel findings reveal that the antimicrobials could cultivate thick-bud and rootless mung bean sprouts due to their toxicity. This study also proposed a new question regarding the abuse of antimicrobials in fast-growing vegetables, which could be a potential food safety issue.Keywords:Vigna radiata;mung bean sprouts;antimicrobials;production;morphology;residues
研究领域:
食品科学
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植物生理学
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DOI
PubMed
高影响因子
TALANTA
·
2021
·
IF 6.057
Yanlei Wang,Feng Ding,Xiaoshuai Sun,Shijin Chen,Huarong Huang,Hong Chen
Hypochlorous acid (HClO) as well as its ionic form (ClO − ), representative of reactive oxygen species (ROS), are essential players in all sorts of biological processes. The abnormal level of each can lead to the onset of various diseases. Besides, Sodium hypochlorite , a commonly-used bleaching agent in our daily lives, could also result in breathing and skin problems when overexposed. Therefore, developing a molecular chemosensor for sensing HClO is of biological and environmental importance. Though many such chemosensors have been reported, new HClO chemosensors with different sensing performances may still come in handy in certain situations. In this work, we have developed a new coumarin-based chemosensor, CM-hbt , for realizing both ratiometric and colorimetric imaging detection of HClO in live cells. Notably, we further explored its application in sensing HClO in plant mung beans as well as fabricated an easy-to-use paper strip apparatus for facilitating its quick detection, which is seldomly seen in other HClO chemosensors. All the analysis results confirmed the high sensitivity and selectivity of this novel chemosensor. DFT calculations were used to decipher the underlying sensing mechanism of CM-hbt . Overall, this work presents a novel chemosensor, CM-hbt , as a colorimetric and ratiometric chemosensor for realizing imaging detection of HClO in a variety of different model systems, which highlights its broad spectrum of application potentials.
研究领域:
生物化学
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分析化学
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环境科学
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2023
·
IF 6.1
Qiqi Quan,Zhiwei Liu,Zhaodong Li,Kangliang Pan,Anastasios Koidis,Yi Lei,Xiaoqin Yu,Qiuhua Mo,Xinan Huang,Hongtao Lei
6-Benzylaminopurine (6-BA), a plant growth regulator with cytokinin-like properties, was recently reported to be illegally used in bean sprouts to increase their commercial appearance. It is still nevertheless challenging to quickly detect this adulteration. In this work, four novel haptens (haptens 1–4) of 6-BA were rationally designed with computer-assisted modeling analysis and then synthesized for use as immunizing haptens to produce antibodies. One of two obtained antibodies showed high sensitivity and specificity toward 6-BA. Based on the most sensitive anti-6-BA antibody, an indirect competitive enzyme-linked immunosorbent assay (icELISA) was performed, which demonstrated a 50% inhibition concentration (IC50) of 1.18 μg/L and a limit of detection of 0.075 μg/L. The average recoveries of this icELISA for 6-BA of spiked samples ranged from 87.2 to 95.0% with a coefficient of variation of less than 8.7%. Furthermore, the blind samples were detected simultaneously by the method and HPLC-MS/MS, and the results showed good agreement with each other. Therefore, the proposed icELISA can facilitate the rapid surveillance screening of adulterated 6-BA in sprout vegetables.
研究领域:
食品安全
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生物化学
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分析化学
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产品关联
DOI
PubMed
高影响因子
Carbon Trends
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2021
·
IF 0
Shaofeng Liang,Muqun Wang,Wei Gao,Sa Luo,Na Huang,Yuxuan Qin
As a nano-fertilizer, carbon dots have good application prospects in crop planting. And its unique fluorescent properties and low toxicity make it become a good biomarker material. In this paper, CDs is prepared by a facile synthesis method. Mung bean as a model plant, and gradient concentration of N-CDs solution for breeding bean sprouts. The results show that 0.15 mg⋅L −1 carbon point solution can promote plant growth. At the same time, we also reveal that carbon dots segmented migration in bean sprouts, discuss the transfer path of carbon dots in bean sprouts, and find that the absorption of carbon dots solution by bean sprouts is linear, and its R 2 =0.98. We find that carbon dots can maintain good fluorescence properties in bean sprouts. A metabolic residue experiment is carried out and it is found that the carbon dots still exist in the bean sprouts after 24 h.
研究领域:
植物学
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农业科学
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材料科学
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DOI
Analytical Methods
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2014
·Vol. 7
·期 2
·684-689
·
IF 1.938
Yuan Su,Li Qi,Xiaoyu Mu,Minglin Wang
In this work, L-histidine-stabilized gold nanoclusters were prepared by a green one-pot process and employed as a selective probe for sensing ferric ions. The obtained probe exhibited bluish green fluorescence, and exhibited an excitation maximum at 394 nm and emission maximum at 492 nm. The fluorescence of the probe was obviously quenched by the introduction of ferric ions, hinting that the synthesized probe could be applied for sensing ferric ions. Further, the selectivity of the proposed probe for sensing ferric ions was investigated. The results indicated that the fluorescent probe had favorable sensitivity and selectivity for the detection of ferric ions. Moreover, this probe was further used for the analysis of ferric ions in bean sprouts. It showed that the gold nanocluster based fluorescent probe has great potential for sensing metal ions in plants.
研究领域:
分析化学
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植物学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
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2020
·
IF 6.306
Shenghuai Hou,Xiaowei Sun,Lizong Chen,Yanzhen Yin,Wenhua Ji
A new amino-modified Scholl-coupling mesoporous polymer (NH 2 @SMPA)-online solid-phase extraction method, coupled with high-performance liquid chromatography (online SPE-HPLC) was established for the analysis of six plant growth regulators (PGRs) in bean sprouts. NH 2 @SMPA was synthesized by acid-catalyzed deacetylation of acetylamino-Scholl-coupling mesoporous polymer (SMPA). The diversity of functional groups, such as aromatic, acetylamino, and NH 2 , was conducive to multiple binding interactions between NH 2 @SMPA and PGRs. NH 2 @SMPA exhibited superior extraction capability for PGRs, compared with SMPA and commercial adsorbents. The extraction conditions, including loading solvent, pH of loading solution, eluting solvent, and flow rates of loading and elution, were optimized. Under the optimized conditions, wide linear ranges (0.01–500 μg kg −1 ) and low detection limits (2.34–20.2 ng kg −1 ) were obtained. The recoveries were satisfactory, i.e., 86.0% to 109% with relative standard deviations ≤9.8% (n = 3). Finally, the online SPE-HPLC method was successfully used for determination of PGRs in bean sprouts.
研究领域:
分析化学
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食品科学
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2018
·
IF 4.946
Hua Zhang,Xingqiang Wu,Yanan Yuan,Dandan Han,Fengxia Qiao,Hongyuan Yan
A new ionic liquid functionalized graphene-pipette-tip solid-phase extraction method coupled with high-performance liquid chromatography was established for the simultaneous extraction and determination of three auxins in soybean sprouts. The graphene adsorbent, with multiple adsorption mechanisms, was first synthesized by functional modification of pentafluorobenzyl imidazolium bromide ionic liquid through thiol-ene click chemistry. The ionic liquid was applied to prevent the aggregation of graphene; it also imbued graphene with the ability for π-π interactions, ionic exchange, electrostatic interactions, as well as hydrogen bonding (which is stronger than the interaction between water and analytes), by augmenting the adsorption mechanisms between the adsorbent and analytes. Under optimized conditions, linearity was achieved in the ranges 0.03–5.00 µg/g for indole-3-acetic acid and 1-naphthaleneacetic acid and 0.09–5.00 µg/g for 2,4-dichlorophenoxyacetic acid, with a detection limit of 0.004–0.026 µg/g; this adsorbent has been successfully applied for the determination of auxins in soybean sprouts.
研究领域:
分析化学
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植物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Taibah University for Science
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2020
·
IF 1.863
Liumin Fan,Xiufang Liu,Yunfang Ma,Qisen Xiang
The present work aimed to investigate the effect of plasma-activated water (PAW) on seed germination and seedling growth of mung bean sprouts. Distilled water was exposed to non-thermal plasma for 15, 30, 60, and 90 s to prepare PAW, which was defined as PAW15, PAW30, PAW60, and PAW90, respectively. The germination rate, growth characteristics, total phenolic and flavonoid contents were all maximized when mung bean seeds were treated by PAW15, then followed by a decline over the prolonged plasma activation time (30–90 s). PAW15 caused no remarkably changes in the antioxidant capacity of sprouts as compared to DW (p > 0.05). However, the antioxidant activity of sprouts prepared with PAW30, PAW60, and PAW90 were decreased significantly (p < 0.05) compared with that of sprouts soaked in distilled water. In summary, PAW may be used to improve the production of sprouts, but the process parameters should be optimized for each application.
研究领域:
植物学
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农业科学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2019
·
IF 4.191
Xianchao Shang,Yuqing Dou,Yingjie Zhang,Jia-Neng Tan,Xinmin Liu,Zhongfeng Zhang
Natural deep eutectic solvents (DESs) have received increasing attention in recent years as emerging green solvents for efficient extraction of bioactive compounds from natural resources. In this study, we aimed to evaluate the potential and effectiveness of DESs with different polarities for extraction of isoflavones from chickpea sprouts. To this end, we examined 20 different DESs based on choline chloride, betaine, and l -proline as hydrogen bond acceptors in combination with different hydrogen bond donors (carboxylic acids, alcohols, sugars, and amine). Extraction efficiency was evaluated based on the extraction yields of four isoflavones (ononin, sissotrin, formononetin, and biochanin A), total flavonoid content, and anti-oxidant activity. On the basis of the results obtained, choline chloride-based DESs containing propylene glycol as a hydrogen bond donor (molar ratio of 1:1) with 30% (v/v) of water was the most effective at extracting isoflavone compounds from chickpea sprouts, compared to conventional solvents. Subsequently, in order to examine the factors contributing to optimal ultrasound-assisted extraction conditions, a Box-Behnken design paired with response surface methodology was implemented. DES water content and extraction temperature were accordingly found to have prominent effects on the extraction yields of isoflavones. Our results indicate that DESs can be used as suitable green solvents for extracting bioactive compounds from various biomaterials.
研究领域:
食品科学
·
植物化学
·
环境科学
✓ 已验证
DOI
高影响因子
TALANTA
·
2017
·
IF 4.162
Wei Wang,Man He,Beibei Chen,Bin Hu
A simple, sensitive and inexpensive method of ion-pair stir bar sorptive extraction (IP-SBSE) combined with high performance liquid chromatography -ultraviolet (HPLC-UV) detection was developed for the simultaneous determination of three acidic phytohormones (salicylic acid (SA), indole-3-acetic acid (IAA), and abscisic acid (ABA)) in vegetables. With the addition of cationic surfactant-cetyltrimethylammonium bromide (CTAB) in sample solutions, ion pair between CTAB and target phytohormones were formed, leading to an improvement of the extraction efficiency for target phytohormones by C 18 silica coated SBSE. The factors affecting the extraction efficiency of three target phytohormones by IP-C 18 coated SBSE were optimized, and the optimal experimental conditions were established. Under the optimal conditions, the limits of detection for SA, IAA and ABA obtained by IP-C 18 -SBSE-HPLC-UV were 2.7, 2.1 and 0.3 μg L −1 , with the relative standard deviations of 6.0%, 5.0% and 8.3%, respectively, and the enrichment factor was 32–49-fold. The developed method was applied to the determination of SA, IAA and ABA in cucumbers and green bean sprouts, and the recoveries of SA, IAA and ABA for the spiked samples were between 72.8% and 109.5%.
研究领域:
分析化学
·
植物学
✓ 已验证
DOI
高影响因子
Innovative Food Science & Emerging Technologies
·
2018
·
IF 3.116
Qisen Xiang,Xiufang Liu,Shengnan Liu,Yunfang Ma,Chunqing Xu,Yanhong Bai
The efficacy of nonthermal plasma-activated water (PAW) in the decontamination of mung bean sprouts was evaluated in this work. After being treated with PAW for 30 min, the populations of total aerobic bacteria and total yeasts and moulds on mung bean sprouts were decreased by 2.32- and 2.84- log 10 CFU/g, respectively. The PAW treatment had no significant effect on the antioxidant potential of mung bean sprouts as shown by using 2,2-diphenyl-1-picrylhydrazyl free radical (DPPH•) scavenging activity assay, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) method, and ferric reducing antioxidant power (FRAP) assay ( p > 0.05). Additionally, the PAW treatment caused no significant changes in the total phenolic and flavonoid contents, nor the sensory characteristics of mung bean sprouts ( p > 0.05). Reactive species such as nitrates, nitrites, and H 2 O 2 were generated in PAW, which presumably contributed to the disinfection efficacy of PAW. These data show that PAW can be used as a promising nonthermal technology for the control of microbial contamination in sprouts. Industrial relevance Edible sprouts are common food ingredients across the world. However, sprouts can be contaminated by pathogenic microorganisms, which may result in health risks to humans. Recently, PAW has been shown to be a safe and effective method for food surface sanitation. However, the application of PAW in the microbial control for sprouts is less investigated. In this study, the influences of PAW on the microbial load , chemical and sensory quality of mung bean sprouts were investigated for the first time. The results showed that PAW could effectively inactivate bacteria and yeasts and moulds on mung bean sprouts without resulting in significant changes in the antioxidant capacities , total phenolic and flavonoid contents, and sensory characteristics of mung bean sprouts. These data indicated that PAW can be used as a promising nonthermal technology for reducing microbial populations on sprouts.
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）