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SCIENCE OF THE TOTAL ENVIRONMENT · 2024 · IF 8.2

Optimized nanopesticide delivery of thiamethoxam to cowpeas (Vigna unguiculata) controls thrips (Megalurothrips usitatus) and reduces toxicity to non-target worker bees (Apis mellifera)

Wenjie Deng,Yanheng Zhang,Liangheng He,Li Xu,Xulang Ye,Hanhong Xu,Li Zhu,Jinliang Jia

Thrips [ Megalurothrips usitatus (Bagnall)] (Thysanoptera: Thripidae) is a pest that poses a serious challenge to global crop production and food supply, especially to the cowpea industry. Nano-delivery systems have broad application prospects in the prevention and control of pests in agriculture. Herein, three types of amino acid (AA) modified polysuccinimide nano-delivery carriers (PSI-GABA, PSI-ASP and PSI-GLU) were constructed with a diameter of approximately 150 nm to load thiamethoxam (THX), which enhanced THX effective distribution and use with cowpea plants. Significantly, the PSI-GLU nanocarrier effectively delivered THX to cowpea plant tissues following 6 h of soil application. Compared with commercial THX suspension (SC), the THX content in the leaves of cowpea plants was increased by 2.3 times. Confocal laser scanning microscopy revealed that the FITC-labeled PSI-GLU nanocarrier reached the leaves through the vascular system after being absorbed by the roots of cowpea plants. The PSI-GLU nanocarrier decreased the LC 50 of THX from 11.45 to 7.79 mg/L and significantly enhanced the insecticidal effect. The PSI-GLU nanocarrier also improved the safety of THX to worker bees at 48 h, and moreover showed a growth-promoting effect on cowpea seedlings. These results demonstrated that the PSI-GLU nano-delivery carrier has promising uses on improving the effective utilization of THX for the sustainable control of thrips and reducing the risk to non-target pollutions.

CAS / MDL: 3326-32-7
研究领域: 农业科学 · 昆虫学 · 植物保护
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ENVIRONMENTAL SCIENCE & TECHNOLOGY · 2023 · IF 11.4

Identification and Coexposure of Neonicotinoid Insecticides and Their Transformation Products in Retail Cowpea (Vigna unguiculata)

Qing Zhang,Yingying Yang,Nanxiu Shang,Yu Xiao,Yilin Xiao,Yanna Liu,Xiaoman Jiang,Edmond Sanganyado,Shaoda Liu,Xinghui Xia

There is growing evidence that the transformation products of emerging contaminants in foodstuffs may pose a health risk to humans. However, the exact identities, levels, and estimated dietary intake (EDI) of neonicotinoid transformation products in crops remain poorly understood. We established an extended suspect screening strategy to investigate neonicotinoid insecticides and their transformation products in retail cowpea from 11 cities in Hainan Province, China. Forty-nine transformation products were identified in retail cowpea, of which 22–36 were found in 98.6% of the samples. Notably, 31 new transformation products were derived from new processes or a combination of different transformation processes. The mean concentrations of neonicotinoids and nine of the transformation products (with authentic standards) were in the ranges of 0.0824–5.34 and 0.0636–1.50 ng/g, respectively. The cumulative EDIs of the quantified transformation products were lower than those of parent neonicotinoids with the exception of clothianidin desmethyl, which had a ratio of 1157%. However, the coexistence of the other 40 transformation products (without authentic standards) in cowpea suggested that the exposure risk from all of the transformation products might be higher. This study demonstrated that pesticide transformation products should be considered in food chain risk assessments and included in future regulatory management.

研究领域: 食品安全 · 环境科学 · 毒理学
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FOOD AND CHEMICAL TOXICOLOGY · 2020 · IF 4.679

A comparative study on nutritive peculiarities of 24 Chinese cowpea cultivars

Zhouya Bai,Xiaojun Huang,Jinxia Meng,Lijiao Kan,Shaoping Nie

The nutritive peculiarities and original distribution of 24 Chinese cowpeas cultivars, achieved from Jiangxi, Jilin, Shanxi and Shaanxi provinces in China, were evaluated. Their nutritional components were determined and nutrient characteristics were discussed. Principal component analysis (PCA) was applied to classify the cowpea samples based on their nutrient composition. The results discovered that the proximate composition varied among 24 cowpeas in protein (17.30–27.23%), fat (1.87–3.14%), high level of dietary fiber (29.49–38.54%) and resistant starch (3.18–31.33%). The total tocopherol varied from 74.20 to 187.07 μg/g. PCA results presented that the 24 cowpeas cultivars were automatically grouped with provinces of origin, indicating a relationship between nutrient composition and cultivating religion for cowpeas. The results of this study provide us the information on the nutrients characteristic of current cowpeas in China, and give a guideline on cowpea cultivating optimization and processing.

CAS / MDL: 4432-31-9 77-86-1
研究领域: 食品科学 · 植物营养学
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PLoS One · 2021 ·Vol. 16 ·期 11 ·e0258508 · IF 3.24

Smartphone-based molecularly imprinted sensors for rapid detection of thiamethoxam residues and applications

Sihua Peng,Aqiang Wang,Yuyang Lian,Xi Zhang,Bei Zeng,Qiulin Chen,Heming Yang,Jinlei Li,Limin Li,Jianguo Dan,Jianjun Liao,Shihao Zhou

In order to achieve rapid detection of thiamethoxam residues in mango, cowpea and water, this study modified the screen printed carbon electrode (SPCE) to make a specific molecular imprinting sensor (Thiamethoxam-MIP/Au/rGO/SPCE) for thiamethoxam. An integrated smartphone platform was also built for thiamethoxam residue analysis. The performance of the complete system was analyzed by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The system was then applied for the rapid determination of thiamethoxam residues in water, mango and cowpea samples. The results showed that the molecular sensor showed good linearity in the range 0.5–3.0 μmol/L of thiamethoxam. The detection limit of thiamethoxam was 0.5 μmol/L. Moreover, the sensor had good reproducibility and anti-interference performance. The average recovery rates of the pesticide residues in water, mango and cowpea samples were in the range of 90–110% with relative standard deviations < 5%. The rapid detection system for thiamethoxam residue constructed in this study was simple, reliable, reproducible and had strong anti-interference. It has broad application prospects in the field detection of thiamethoxam residue, and serves as a valuable reference for the further development of rapid detection technology of pesticide residues in the field of environment and food safety.

研究领域: 分析化学 · 食品安全
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PLANT CELL · 2024 · IF 10

A viral small interfering RNA-host plant mRNA pathway modulates virus-induced drought tolerance by enhancing autophagy

Wu Xinyang,Chen Shuting,Zhang Zixin,Zhou Weixin,Sun Ting,Ning Kang,Xu Min,Ke Xubo,Xu Pei

Virus-induced drought tolerance presents a fascinating facet of biotic-abiotic interaction in plants, yet its molecular intricacies remain unclear. Our study shows that cowpea mild mottle virus (CPMMV) infection enhances drought tolerance in common bean (Phaseolus vulgaris) plants through a virus-derived small interfering RNA (vsiRNA)-activated autophagy pathway. Specifically, a 21-bp vsiRNA originating from the CPMMV Triple Gene Block1 (TGB1) gene targeted the 5' untranslated region (UTR) of the host Teosinte branched 1, Cycloidea, Proliferating Cell Factor (TCP) transcription factor gene PvTCP2, independent of the known role of TGB1 as an RNA silencing suppressor. This targeting attenuated the expression of PvTCP2, which encodes a transcriptional repressor, and in turn upregulated the core autophagy-related gene (ATG) PvATG8c, leading to activated autophagy activity surpassing the level induced by drought or CPMMV infection alone. The downstream EARLY RESPONSIVE TO DEHYDRATION (ERD) effector PvERD15 is a homologue of Arabidopsis thaliana AtERD15, which positively regulates stomatal aperture. PvERD15 was degraded in PvATG8c-mediated autophagy. Therefore, we establish a TGB1-PvTCP2-PvATG8c-PvERD15 module as a trans-kingdom fine-tuning mechanism that contributes to virus-induced drought tolerance in plant-drought-virus interactions.

CAS / MDL: 5142-23-4
研究领域: 植物病毒学 · 分子生物学 · 植物生理学
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JOURNAL OF COLLOID AND INTERFACE SCIENCE · 2019 · IF 6.361

Functionalization of polyethylene terephthalate knitted fabric with cowpea protein and biopolymer complex: Applications for enhancing wettability and UV-Protection properties

Hashim Sliman,Xue Dong,Tao Zhao

In recent years, increasing environmental awareness and human skin protection from harmful radiation of the sun has attracted attention amongst numerous researchers. Here, a simple strategy was developed to enhance the hydrophilicity and ultraviolet (UV) protection of polyethylene terephthalate (PET) knitted fabric surface via grafting green cowpea protein (CP) with gum arabic modified epoxy (GAE) as a cross-linking agent. Chemical grafting between pretreated PET and GAE as well as between GAE and CP improved the hydrophilicity and UV protection of PET knitted Fabric. The enhanced surface wettability was confirmed by the decreased water contact angle from 135° to zero °. Moreover, the UV protection factor rating of modified PET knitted fabric was higher, indicating that the modifier layer caused scattering and absorbance of UV light. This approach has shown promising results for improving hydrophilicity and UV protection with sustained mechanical durability of 7 washing cycles.

研究领域: 材料科学 · 纺织化学 · 表面工程 · 生物高分子 · 功能纺织品
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JOURNAL OF ECONOMIC ENTOMOLOGY · 2025 · IF 2.4

Volatiles from non-host plant Baccaurea ramiflora (Malpighiales: Phyllanthaceae) attract cowpea thrips, Megalurothrips usitatus (Thysanoptera: Thripidae)

Yan Haoming,Qiu Haiyan,Zhao Jing,Han Dongyin,Fu Yueguan,Zhou Aiming,Chen Junyu,Li Lei

The cowpea thrips Megalurothrips usitatus (Bagnall) (Thysanoptera: Thripidae) exhibited unexpected aggregation on flowers of the non-host Baccaurea ramiflora Lour. (Malpighiales: Phyllanthaceae), suggesting floral volatile-mediated attraction. We systematically investigated this phenomenon through: (i) GC-MS identification of B. ramiflora floral volatiles; (ii) electroantennographic (EAG) and olfactory assays; and (iii) field trapping experiments. Both the flowers of B. ramiflora and their headspace extracts exhibited significant attractive effects on M. usitatus. Five key volatile organic compounds were consistently detected: linalool, 2,4-decadienal, trans-2-decenal, (Z)-β-ocimene, and trans-2-undecenal. The antennae of M. usitatus demonstrate distinct sensitivities to different volatiles, with the appearance of their EAG response peaks being affected by the dosage levels at which these volatiles are applied. The olfactory responses revealed that linalool (100 to 10000 μg), 2,4-decadienal (10 to 10000 μg), and trans-2-undecenal (1000 to 10000 μg) attracted M. usitatus. Trans-2-decenal attracted M. usitatus at 1000 μg but repelled at 10000 μg. (Z)-β-ocimene repelled M. usitatus at doses ranging from 10 to 10000 μg. Field trials confirmed 2,4-decadienal (10 to 100 μg) as the most effective attractant. These findings support the development of a 2,4-decadienal-based semiochemical strategy for sustainable M. usitatus management in cowpea production systems.

CAS / MDL: 7440-44-0 64365-11-3
研究领域: 昆虫学 · 化学生态学 · 农业昆虫与害虫防治 · 植物-昆虫互作 · 行为生态学 · 挥发性有机化合物分析
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FOOD SCIENCE AND TECHNOLOGY RESEARCH · 2021 · IF 0.668

Changes in phenolic composition and bioactivity of raw and pickled cowpea (Vigna unguiculata L. Walp.) green pod after in vitro simulated gastrointestinal digestion

Shirong Huang,Huan Liu,Sinian Yan,Dongfang Chen,Xin Mei

Here, the changes in phenolic composition and antioxidant activities (ABTS, DPPH, and FRAP) of raw and pickled cowpea green pod during in vitro simulated gastrointestinal digestion (GID) were investigated. The inhibitory activities of the undigested and digested samples towards α-amylase, α-glucosidase and lipase were determined. Results revealed that total phenolic (TP) and flavonoid (TF) contents of both raw and pickled samples were increased after gastric and intestinal digestion, and the pickled sample had lower TP and TF content after each digestion phase. A similar trend was observed for antioxidant activities, which were significantly correlated with TP and TF contents (p < 0.05). Seven phenolic compounds were identified and quantified by HPLC-DAD. Their contents were affected by digestion and pickling, and raw cowpea pod had higher contents before and after GID. Inhibitory effects towards α-amylase, α-glucosidase and lipase were increased for raw cowpea pod after GID. Pickled cowpea pod also showed an increased inhibitory activity towards lipase, while no activity towards α-amylase and α-glucosidase. Raw cowpea pod had higher inhibitory effects towards α-amylase, α-glucosidase and lipase than the pickled one at each digestion phase. Our results showed that pickling process reduced the health-promoting properties of cowpea green pod.

CAS / MDL: 109-99-9
研究领域: 食品科学 · 营养学 · 生物化学 · 药理学
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FOOD CHEMISTRY · 2025 · IF 9.8

Vacuum-steam pulsed blanching enhances drying, nutrient retention, and anti-nutritional factor reduction in Vigna unguiculata

Samuel Ariyo Okaiyeto,Shi-Han Yu,Haiou Wang,Jin-Hong Zhao,Cun-Shan Zhou,Ahmed Jamilu Mustapha,An-An Zhang,Fan Yang,Jing-Shou Zhang,Hong-Wei Xiao

This study evaluates vacuum-steam pulsed blanching (VSPB) as a pretreatment for Vigna unguiculata pods, focusing on drying kinetics, nutritional quality, and anti-nutritional factors. Pods underwent 0–4 VSPB cycles, followed by hot air drying at 50, 60, and 70 °C. VSPB reduced peroxidase activity to 4.8 %, enhancing enzyme inactivation. Texture analysis revealed an 85.6 % reduction in hardness, while the Midilli model provided the best description of drying kinetics (R 2  ≥ 0.9979). Specific energy consumption decreased by up to 40 % at 70 °C for blanched samples. VSPB preserved ascorbic acid, vitamin A, and chlorophyll, reduced lignin by 29.63 %, and lowered lectins from 71.01 to 8.88 Hu/mg. Folate retention declined with increased cycles. SEM and TEM analyses revealed microstructural and ultrastructural disruptions enhancing moisture diffusivity. VSPB proves effective in reducing anti-nutritional factors and retaining nutrients, offering a promising pretreatment for Vigna unguiculata with potential industrial applications.

研究领域: 食品科学与技术 · 采后加工 · 植物食品生物化学 · 干燥技术 · 营养品质
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