INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Rui Xue,Juan Wang,Weiwei Huang,Yibin Qiu,Hong Xu,Rui Wang,Sha Li
Mussel foot proteins (mfps) play important roles in surface interaction and underwater adhesion. However, limited production and the lack of adhesion of recombinant mfps have restricted their widespread use. Here, we present a general strategy for enhancing both the expression and function of mfps by connecting multiple protein fragments. We demonstrate that fp-535, the fusion protein comprising fp-5 at each fp-3 terminus, exhibits best adhesion strength up to 1.43 MPa and coating ability, while achieving a higher yield of 91.13 mg/L in the flask culture. After optimizing the fermentation parameters such as temperature and volume as well as the culture medium, the yield of fp-535 was increased to 1.70 g/L in a 5-L bioreactor. Our approach highlights the advantage of fusion mfps and provides potential for the sustainable supply of practical bioadhesive materials.
研究领域:
生物化学
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海洋生物学
·
生物技术
✓ 已验证
DOI
PubMed
高影响因子
Journal of Materials Chemistry A
·
2014
·Vol. 3
·期 6
·2650-2657
·
IF 0
Zhen-Xing Wang,Cher-Hon Lau,Nai-Qing Zhang,Yong-Ping Bai,Lu Shao
Novel hybrid coatings with excellent wettability are architecturally constructed on the surfaces of different types of separation membranes via simultaneous polymerization of mussel-inspired dopamine and hydrolysis of commercially available and low-cost silane through a highly efficient one-step approach. After coating with the designed hybrid coatings, the ultrafiltration (UF) membranes possess high hydrophilicity and excellent dry storage ability while the microfiltration (MF) membranes are endorsed with superhydrophilicity and underwater superoleophobicity. Such unique UF and MF membranes can be deployed for treating protein-rich water with drastically enhanced functions and separating oily water (oil-in-water emulsion) under atmospheric conditions with ultrahigh water flux and superior antifouling abilities. This versatile strategy to tailor membrane surface wettability paves the way for separation membranes to be used in harsh water environmental remediation and greatly stimulates the rapid development of mussel-inspired pDA based hybrid materials for advanced applications.
研究领域:
材料科学
·
环境科学
·
生物仿生学
✓ 已验证
DOI
DYES AND PIGMENTS
·
2018
·
IF 3.767
Li Li,Feifei Zhao,Tianyi Zhang,Changli Lü
We developed a simple way to prepare the polymer functionalized N -doping carbon dots (CDs) inspired by the mussel chemistry for white LED application. The N -doping CDs were decorated by catechol-terminated hydrophilic poly(poly(ethylene glycol) methyl ether methacrylate ( PPEGMA) and hydrophobic polystyrene (PS) through Schiff base condensation/Michael addition reaction (CD@PEGMA and CD@PS), which owns two advantages: (1) polymer anchored CDs improved the optical properties of pure CDs; (2) hydrophobic PS could transfer hydrophilic CDs into nonpolar organic solvents to expand the application range of water-soluble CDs. The white-light bulk nanohybrid was obtained via in-situ polymerization of styrene monomers containing CD@PS as blue component and 5-(2-methacryloyl-ethyloxymethyl)-8-quinolinol (MQ) coordinated CdSe/ZnS quantum dots (QDs) as green and red components. The warm white light-emitting diode (WLED) was also fabricated based on the white bulk nanomaterials with CIE coordinate (0.32, 0.31) and the correlated color temperature 6250 K.
研究领域:
材料科学
·
光学工程
✓ 已验证
DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2024
·
IF 6.2
Chuanyue Chen,Xueer Wang,Yingrong Jin,Yinchang Yao,Suchang Li,Zhi Liao,Xiaolin Zhang,Xiaojun Yan
The increasing deposition of microplastics (MPs) in aquatic ecosystems is a worldwide concern. MPs can interact with other environmental pollutants, such as heavy metals, and change their toxicity. In this study, we focused on the effects of MPs and lead (Pb), as a toxic heavy metal, on marine mussel Mytilus coruscus under separate and co-exposure situations at environmentally relevant concentrations: MPs (1 mg/L) and Pb (50 μg/L). We found that MPs alone or in combination with Pb significantly decreased the respiration and filtration rates of the mussels ( p < 0.05). Histological observations revealed varying extents of damage to the gill and digestive gland caused by a single exposure to MPs, which was aggravated by co-exposure to Pb. In addition, co-exposure induced a higher level of oxidative stress, which was reflected by an increase in hydrogen peroxide and malondialdehyde content, and a decrease in antioxidant enzyme activity. Meanwhile, co-exposure poses a significant threat to the immune function of the mussels, as evidenced by induction of hemocytes to produce excess reactive oxygen species (ROS), significantly reducing lysosome activity ( p < 0.05), inhibiting the expression of autophagy-related genes, and inducing the expression of apoptosis-related genes, resulting in hemocyte apoptosis. Furthermore, the TLR/MyD88/NFκB signaling pathway is involved in the immune response of mussels to environmental stress. This study provides novel perspectives on the toxicity of MPs combined with Pb in marine animals, as well as the molecular mechanisms underlying their ecotoxicological effects.
研究领域:
环境科学
·
毒理学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2018
·
IF 6.735
Yan Kang,Huijun Xie,Bo Li,Jian Zhang,Huu Hao Ngo,Wenshan Guo,Zizhang Guo,Qiang Kong,Shuang Liang,Jie Liu,Tingting Cheng,Liwei Zhang
The role of mussels in laboratory-scale constructed wetlands (CWs) where wastewater was contaminated with five typical polycyclic aromatic hydrocarbons (PAHs) (three 3-ring PAHs and two 4-ring PAHs) was investigated in this study. The CWs performance and PAHs removal mechanisms were well studied. Results indicated that these five PAHs improved removal efficiencies of NO 3 -N but in the case of NH 4 -N accumulation occurred. Of the five added PAHs, the 4-ring PAHs were more refractory with higher concentrations in effluent than 3-ring PAHs. By monitoring the five PAHs concentration in water, mussels had excellent removal efficiency of the five PAHs (97%). According to the mass balance calculation, mussels promoted plant uptake of five PAHs, contributing 15.2% of five PAHs removal in CWs. The PAHs could also accumulate in mussels through ingested substrate. Thus, mussels presented a positive correlation with five PAHs purification at a depth of 0–10 cm in substrate, which was 34.7 μg/kg lower than the control group. Due to the purification and enhanced aerated degradation, mussels performed better in removing 3-ring PAHs in substrate, decreasing 8.3% for 3-ring PAHs compared to the control. A positive correlation between five PAHs addition and nirS , nrfA genes was observed. However, PAHs showed negative impact on nitrifying bacteria ( amoA ). The significant correlations between mussels and five PAHs made it possible to improve CWs for PAHs treatment with aquatic faunas.
研究领域:
环境科学
·
生态学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2025
·
IF 2.8
Jine Wang,Rui Zhao,Zhe Cheng,Pei Wang,Meihua Jin,Jiujun Xu
As a kind of natural polyphenolic antioxidants, tannic acid (TA) has a wide range of sources and biosafety, excellent antibacterial properties and its special chemical structure makes it easy to bind to the surface, which may provide inspiration for the development of antifouling materials. In this paper, a TA-based antifouling coating was designed and prepared. A stable intermediate transition layer was formed by co-deposited 3,4-dihydroxy phenyl-L-alanine (DOPA) and polyethyleneimine (PEI) on the surface of polyvinyl chloride (PVC), and then TA molecules were grafted on the surface to prepare a TA functionalized (PVC/(PDA/PEI)-TA) coating. The effect of the coating on the adhesion of marine bacteria, benthic diatoms and mussels was investigated. The results indicated that the coating had good antifouling performance. Marine bacteria and benthic diatoms had a low adhesion rate on the coating surface and were prone to the detachment under water flow erosion. The TA on the coating surface affected the adhesion behavior of mussels, which only secreted a small amount of adhesive plaques on the surface, and the adhesion force between the plaques and the substrate became low. This study may provide new solutions to marine biofouling.
研究领域:
材料科学
·
海洋生物学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2021
·Vol. 138
·期 46
·51382
·
IF 3.125
Longfang Ren,Congcong Lin,Pingchuan Lei
A series of waterborne polyurethane (PDMAPU) containing catechol group were prepared by double-bond random copolymerization of terminated double bond polyurethane prepolymer with modified acrylamido dopamine under thermal initiation. This kind of mussel-like waterborne polyurethane adhesive is inspired by marine mussel, as well as it is environment-friendly and used in bonding of leather. FTIR and 1 H NMR proved the successful introduction of catechol group into polyurethane matrix. Oxidative cross-linking between catechol unit and molecular chain in PDMAPU structure, the thermal stability and crystallization ability of PDMAPU was significantly improved. The increase of the particle size of PDMAPU emulsion showed that the introduction of catechol group changed the microstructure of polyurethane and enhanced the cross-linking degree. The water resistance of polyurethane emulsion was further improved. Compared with PU without catechol group, the peel strength of leather substrate adhered by PDMAPU emulsion increased from 0.42 to 1.93 MPa, which indicated that PDMAPU has better bonding properties with leather. The bidentate hydrogen bond formed with catechol group as the reaction sites is considered to be the key reason for the adhesion of mussel-like polyurethane adhesive to hydrophilic substrates. This work provides an alternative to prepare environment-friendly high performance adhesive for hydrophilic substrates.
研究领域:
材料科学
·
化学工程
·
生物仿生学
✓ 已验证
产品关联
DOI
高影响因子
Bioactive Materials
·
2025
·
IF 20.3
Zeyu Du,Yuting Huang,Qing Ma,Wentai Zhang,Yan Fu,Nan Huang,Xin Li,Zhilu Yang,Wenjie Tian
Surfaces with enduring and superior antithrombotic properties are essential for long-term blood-contacting devices. While current surface engineering strategies integrating anticoagulants and antiplatelet agents show promise in mimicking the non-thrombogenic properties of the endothelium, their long-term effectiveness remains limited. Here, we report an easy-to-perform, dual-biomimetic surface engineering strategy for tailoring long-acting endothelium-mimicking anti-thrombotic surfaces. We first designed a Mytilus edulis foot protein-5 (Mefp-5) mimic rich in amine and clickable alkynyl groups to polymerize-deposit a chemical robust coating onto the surface through a mussel-inspired adhesion mechanism. Then, a clickable nitric oxide (NO, an antiplatelet agent)-generating enzyme and the anticoagulant heparin were sequentially co-grafted onto the chemical robust coatings via click chemistry and carbodiimide chemistry. Our results demonstrate that this engineered surface achieved an impressive NO catalytic release efficiency of up to 88 %, while heparin retained 86 % of its bioactivity even after one month of exposure to PBS containing NO donor. Both in vitro and in vivo experiments confirmed that this robust endothelium-mimicking coating substantially reduces thrombosis formation. Overall, our long-acting endothelium-mimicking anti-thrombotic coatings present a promising and feasible strategy to address thrombosis-related challenges associated with blood-contacting devices.
研究领域:
生物材料学
·
医学工程
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2018
·Vol. 54
·期 4
·3648-3660
·
IF 2.993
Liao Xiaofeng,Li Hongqiang,Su Xiaojing,Zhan Haomiao,Lai Xuejun,Zeng Xingrong
With the deteriorating environmental pollution by oil spill accidents and industrial sewage emissions, stimuli-responsive superwettable materials have been of considerable interest for its intriguing application in oil–water separation. Herein, we report a facile room temperature method to fabricate mussel-inspired cotton fabric with pH-responsive superwettability, by combining in situ generation of silver particles to construct roughness with self-assembly of long alkyl thiols terminated with methyl and carboxyl groups to endow hydrophobicity and responsiveness. The fabricated pH-responsive cotton fabric showed superhydrophobicity with water contact angle (WCA) up to 153° under acidic and neutral conditions while superhydrophilicity with WCA at 0° under alkaline condition. Importantly, the cotton fabric was successfully applied in bidirectional oil–water separation, exhibiting high separation efficiency and excellent reversibility. Furthermore, the pH-responsive cotton fabric also demonstrated superior self-cleaning property and antibacterial activity to E. coli and S. aureus . Our method to construct pH-responsive cotton fabric is simple, and no special techniques, chemicals or control of atmosphere is required. Our findings conceivably stand out as a new tool to fabricate functional superwettable materials and surfaces with responsiveness to external stimuli for various oil-pollution treatments and other potential applications.
研究领域:
材料科学
·
环境科学
·
表面化学
✓ 已验证
产品关联
DOI
高影响因子
AQUATIC TOXICOLOGY
·
2019
·
IF 3.794
Ting Wang,Xizhi Huang,Xiaoyu Jiang,Menghong Hu,Wei Huang,Youji Wang
Titanium dioxide nanoparticles (TiO 2 NPs) are widely used in various products and inevitably released with different sizes and forms into aquatic environment. The purpose of this study was to assess the differential immune toxicity of TiO 2 NPs with size difference on mussel hemocytes using flow cytometry (FCM) assays. Hemocyte parameters, including total hemocyte count (THC), hemocyte mortality (HM), phagocytosis activity (PA), lysosomal content (LC), esterase activity (EA), mitochondrial number (MN), mitochondrial membrane potential (MMP) and reactive oxygen species content (ROS) were evaluated in the mussels Mytilus coruscus exposed to two types of TiO 2 NPs (25nm & 100nm: 0.1, 1, 10 mg/L, respectively). In general, size- and concentration-dependent toxicity was pronounced with 25nm-NP and highest concentration (10mg/L) being the most toxic. Alhough a slight recovery from the TiO 2 exposure was observed, significant carry-over effects were still detected. These results highlight the importance of differential size effects of metal oxide NPs on toxicity mechanisms in aquatic animals.
研究领域:
环境科学
·
免疫学
✓ 已验证
DOI
PubMed
高影响因子
BIOMACROMOLECULES
·
2024
·
IF 5.5
Biwei Qiu,Qianqian Cheng,Rukun Chen,Chunling Liu,Jinchao Qin,Qixia Jiang
Biodegradable stents are the most promising alternatives for the treatment of cardiovascular disease nowadays, and the strategy of preparing functional coatings on the surface is highly anticipated for addressing adverse effects such as in-stent restenosis and stent thrombosis. Yet, inadequate mechanical stability and biomultifunctionality limit their clinical application. In this study, we developed a multicross-linking hydrogel on the polylactic acid substrates by dip coating that boasts impressive antithrombotic ability, antibacterial capability, mechanical stability, and self-healing ability. Gelatin methacryloyl, carboxymethyl chitosan, and oxidized sodium alginate construct a double-cross-linking hydrogel through the dynamic Schiff base chemical and in situ blue initiation reaction. Inspired by the adhesion mechanism employed by mussels, a triple-cross-linked hydrogel is formed with the addition of tannic acid to increase the adhesion and antibiofouling properties. The strength and hydrophilicity of hydrogel coating are regulated by changing the composition ratio and cross-linking degree. It has been demonstrated in tests in vitro that the hydrogel coating significantly reduces the adhesion of proteins, MC3T3-E1 cells, platelets, and bacteria by 85% and minimizes the formation of blood clots. The hydrogel coating also exhibits excellent antimicrobial in vitro and antiinflammatory properties in vivo, indicating its potential value in vascular intervention and other biomedical fields.
研究领域:
生物医学工程
·
材料科学
·
心血管医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2019
·
IF 7.015
Jincheng Ding,Huiqing Wu,Peiyi Wu
A mussel-inspired sulfonated dopamine (SDA) was synthesized and used as an aqueous monomer to cross-link with trimesoyl chloride (TMC) via interfacial polymerization to prepare nanofiltration membranes . The sulfonic acid groups of SDA were credited with the enhancement of membrane performance , as a result of restraining the process of interfacial polymerization and oxidative-polymerization. Moreover, it also endowed these nanofiltration membranes with more hydrophilic , negatively-charged and smoother surfaces. The optimized nanofiltration membranes (NF-SDA-0.1) exhibited a water flux as high as 62.2 L∙m −2 ∙h −1 (0.6 MPa), while maintained applicable salt rejection of Na 2 SO 4 (~90.0%) and dye rejections of Congo red, Methyl blue, Methylene blue and Brilliant green (all > 99.9%). Thus, the sulfonated dopamine was considered as a promising candidate as aqueous phase monomer for the preparation of high-performance nanofiltration membranes.
研究领域:
材料科学
·
环境工程
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）