LWT-FOOD SCIENCE AND TECHNOLOGY
·
2020
·
IF 4.006
Qian Wu,Kuoquan Zhao,Yuanyuan Chen,Jinsong Xiao,Mengzhou Zhou,Dongshen Li,Nianjie Feng,Chao Wang
In this study, a glucose-lysine (G-L) model was selected to simulate the dietary system (cooking of alcohol-cured steak) to study the effect of alcohol concentration on the formation of advanced glycosylation products (AGEs) in Maillard reaction . It was first reported that higher alcohol concentrations accelerated the formation of AGEs as well as their intermediates glyoxal (GO) and methylglyoxal (MGO). The contents of GO and MGO increased from 11.74 ± 2.55 to 50.18 ± 0.16 μg/mL and 19.04 ± 1.25 to 58.51 ± 2.56 μg/mL with increasing ethanol concentration, respectively. High-alcohol samples produced higher levels of Nε-(carboxymethyl) lysine (CML) and Nɛ-carboxyethyl lysine (CEL) detected by U-HPLC-MS/MS. The capacity of catechin to inhibit the formation of AGEs and intermediates were significantly related to the concentration of ethanol, which may be due to ethanol accelerated catechin degradation and weakened the ability of catechin to trap GO and MGO. Our study strongly suggests that although we cannot completely avoid the AGEs produced by the Maillard reaction , in daily life, we try to avoid adding alcohol, especially high-concentration alcohol, when cooking and processing ingredients.
研究领域:
食品科学
·
化学
·
营养学
✓ 已验证
DOI
高影响因子
RESEARCH ON CHEMICAL INTERMEDIATES
·
2020
·Vol. 47
·期 2
·663-682
·
IF 2.262
Zhu Lin,Zheng Xingwen,Zeng Xianguang,Gong Min,Guo Lei
The inhibitive effect and inhibition mechanism of l -lysine derivatives, namely α- N -benzyloxycarbonyl- l -lysine (α-NL) and ε- N -benzyloxycarbonyl- l -lysine (ε-NL), on the corrosion of carbon steel in 0.5 M H 2 SO 4 solution were investigated by electrochemical technique, weightlessness test, scanning electron microscope, atomic force microscope, infrared attenuated reflectance spectroscopy and quantum chemical calculation. The results showed that α-NL and ε-NL as moderate cathodic inhibitors mainly inhibited the cathodic reaction of carbon steel in 0.5 M H 2 SO 4 solution, and the terminal substituted ε-NL showed better corrosion inhibition than the α- N -benzyloxycarbonyl protected α-NL. Their inhibition efficiencies increased with the increase in inhibitor concentration, and the inhibition efficiency of α-NL and ε-NL with concentration of 10 mmol can reach 53.4% and 61.5%, respectively, but decreased with rising temperature. The adsorption of investigated inhibitors on the surface of carbon steel obeyed the modified Langmuir isotherm and El-Awady thermodynamic-kinetic model, and the thermodynamic and kinetic parameters were determined to discuss the mechanism of inhibition of α-NL and ε-NL. Moreover, Quantum chemical calculation gave further insight into the inhibition mechanism of α-NL and ε-NL and was employed to reveal the reasons for their differences in inhibition performance.
研究领域:
材料科学
·
电化学
·
量子化学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Lichun Liu,Lei Liu,Jianhua Xie,Mingyue Shen
The dietary advanced glycation end products (AGEs) contribute to the development of major chronic diseases. Maillard reactions are the main mechanism for AGEs formation but their formation involving ascorbic acid (AA) is far from being fully understood. This study investigated the effect of pH (6–10) and temperature (65, 100 and 120 ℃) on AGEs formation in three model systems: glucose (Glu) + lysine (Lys), AA + Lys and Glu + Lys + AA. In addition, the formation pathway of AGEs in Glu + Lys + AA model system was proposed by carbon module labeling (CAMOLA) technique. The results suggested alkaline environment can promote the production of N -carboxymethyllysine (CML) and N -carboxyethyllysine (CEL), but inhibit the production of pyrraline (Pyr). Meanwhile the high temperature favored AGEs formation. In the U- 13 C-Glu + Lys + AA model, AA produced glyoxal (GO), methylglyoxal (MGO), CML and CEL, which was significantly higher than with Glu alone. This study provides a theoretical basis for the formation mechanism of AGEs in the Maillard reaction involving AA.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Pengxu Wang,Qing Liu,Wenliang Fu,Chao Zhang,Guiling Cai,Weiwei Xing,Hongliang Dai,Hongya Geng,Donggang Xu
Marine mussels have evolved the secretion of diversified mussel foot proteins to firmly attach to wet, salt-crusty, corroded, and fouled surfaces. Biomimicry of this robust wet adhesion via translating sticky biological molecules is still at an impasse mainly due to the lack of available materials and methodology to fully validate the underline mechanisms of adhesion and cohesion. Increasing sources of evidence have shown that the pair structure of protonated 3,4-dihydroxyphenylalanine (DOPA) and lysine contributes to the adhesion on substrates. However, it is still an uncultivated field to study the roles of those commonly distributed lysine and non-lysine pair structures in the adhesion to different surfaces, while remaining the intermolecular cohesion strength of the pair structures. Herein, the adhesive and cohesive properties of precisely designed single-molecule domains were systematically analyzed using single-molecule force spectroscopy after surface modification of probe tips. It was revealed a record-high adhesion force of ∼ 1000 pN for DOPA-lysine on cation-rich substrates (mica) and tyrosine-Lys on cation-deficient substrates (hydrophilic modified silicon nitride, Si 3 N 4 (OH)) due to an identified hydrophobic force. DOPA-phenylalanine and DOPA-tryptophan pairs were found to show versatile strong adhesion to both cation-rich and -deficient substrates, spanning from 750 pN to 2250 pN. Furthermore, our results revealed that Phe-Lys had an extraordinarily robust cohesion strength, up to 12,000 pN due to cation-π interactions. This study broadens our view of peptides adhesion, provides single molecule proofs toward understanding the mechanism of pair structures in natural adhesive proteins, as well as the design principles of the artificial adhesive layer.
研究领域:
材料科学
·
生物物理学
·
仿生学
·
粘附科学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2016
·Vol. 4
·期 31
·5256-5264
·
IF 4.872
Guanglong Ma,Weifeng Lin,Zhen Wang,Juan Zhang,Haofeng Qian,Liangbo Xu,Zhefan Yuan,Shengfu Chen
Protein molecules, which typically have a hydrophobic core and a zwitterionic shell with a polypeptide backbone, could be ideal materials for nanodrug vehicles (NDVs) with low side effects. Here, we synthesized poly(L-aspartic acid(lysine))-b-poly(L-lysine(Z)) (PAsp(Lys)-b-PLys(Z)) (PALLZ), a novel amphiphilic block polypeptide with key structures of protein to investigate the possibility for use as a NDV. This polypeptide can spontaneously self-assemble into micelles in aqueous solution with a zwitterionic brush (the PAsp(Lys) part) to provide the nonfouling shell and a hydrophobic core (the PLys(Z) part) for loading hydrophobic drugs. The doxorubicin (DOX) loaded PALLZ micelles showed excellent resistance to nonspecific protein adsorption in FBS, which leads to very low internalization. Moreover, PALLZ micelles showed no cytotoxicity to MCF7, HeLa and HepG-2 cells up to 500 μg mL−1. All these results indicated that zwitterionic amphiphilic block polypeptides could be promising materials for NDVs.
研究领域:
生物材料学
·
药物递送系统
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2020
·
IF 2.734
Xiaojie Song,Kangli Lan,Qi-Yan Lv,Hongmin Liu,Hui-Fang Cui
Design and fabrication of multifunctional nano-carrier is a promising tool in the field of nanomedicine for glioma theranostics. In this study, blue color nanoparticles with targeted tumor cell staining and drug delivery capabilities were simply fabricated by crosslinking lysine (Lys) and lysine-folic acid (Lys-FA) mixture with genipin as the crosslinking agent through a reversed microemulsion method. Doxorubicin (DOX) as the model drug was loaded through electrostatic interaction. The nanoparticles before (Lys-FA NPs) and after (Lys-FA/DOX NPs) loading of DOX were characterized by ultraviolet–visible (UV–Vis) spectroscopy, Fourier transform infrared spectroscopy (FTIR), dynamic laser light scattering (DLS), transmission electron microscopy (TEM), and fluorescence spectroscopy. Ultimately, the glioma cell staining ability of Lys-FA NPs and cytotoxicity of Lys-FA/DOX NPs were tested in vitro . The results showed that the particle size of Lys-FA NPs can be tuned by adjusting the ratio of Lys to Lys-FA simply. The Lys-FA NPs exhibited dark blue and the blue color is stable under different pH values and temperatures. The feature makes the staining glioma cell pellets visible to naked-eyes. The model drug DOX was loaded on Lys-FA NPs indicated by the increase of size and zeta potential , and change of optical properties. The DOX loaded on the Lys-FA NPs can be sustainedly released, other than burst release. Encouragingly, the Lys-FA NPs can deliver DOX into cells and dramatically reduce cell viability, as proved by U87 cell cytotoxicity analysis and confocal fluorescence imaging. In addition, the Lys-FA NPs (≤2.5 mg mL −1 ) are nontoxic to CHO cells. In a short, the blue color Lys-FA NPs can visibly stain glioma cell pellets and deliver DOX into glioma cells, which can potentially be applied as a multifunctional nanocarrier for tumor imaging and drug delivery.
研究领域:
纳米医学
·
癌症研究
·
药学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Dan Chang,Zhonghua Zhao,Hu Shi,Jianyang Feng,Yongxing Yang,Lihong Shi
Ratiometric fluorescent probes with wavelength-resolved dual target sensing capacity are highly desirable for the simultaneous recognition of target objects. A novel fluorescent carbon dots (CDs) with a yield of 32% was simply synthesized by hydrothermal treatment of neutral red and urea, resulting in discrete dual-emission bands (440 nm/542 nm) under single-wavelength excitation of 380 nm. It was discovered that the fluorescence (FL) signal of CDs at 440 nm enhances with the addition of L -Lysine, whereas the FL intensity at 542 nm remains invariable. Surprisingly, no significant luminescence response is observed after the addition of D -Lysine, implying that as-prepared CDs can serve as fluorescent nanoprobes for chiral recognition of Lysine. On the contrary, it was discovered that the FL band at 542 nm is highly sensitive to pH in linear ranges of 2.0–3.4 and 3.4–8.0, while the FL band at 440 nm is inert to it, providing an effective tool for the ratiometric detection of pH in a “turn-on” mode. Confocal imaging experiments further verify that this nanoprobe can monitor L -Lysine and pH fluctuations in biological systems in real time using ratiometric methods. We believe that our research will provide new insight into the suitable construction of prompt and straightforward ratiometric fluorescent probes for the simultaneous differentiation of two analytes .
研究领域:
分析化学
·
材料科学
·
生物技术
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
POLYMER INTERNATIONAL
·
2023
·Vol. 72
·期 8
·720-726
·
IF 3.2
Jie Tian,Nan Wang,Wenxiu Teng,Shuang Song,Jie Pang,Chunhua Wu,Chengrong Wen
The binding of oppositely charged polyelectrolytes is a well-known gel strategy, and pH is an important factor affecting the gel properties. In this work, the influence of pH (3, 5, 7, 9, 11) on the rheological and textural properties, molecular structure and micromorphology of binary mixed gels of ι -carrageenan (IC) and ε -polylysine (PLL) or ε -polylysine hydrochloride (PLH) was investigated. The results showed that a change in pH led to a change in the properties of binary mixed gels. The lower the pH, the higher were G ′, G ″, complex viscosity and gel transition temperature. At the same time, the water holding capacity and gel strength of IC/PLL (IP) and IC/PLH (IPH) were also improved accordingly, and the gel properties of IPH were always better than those of IP. As the pH increased to alkalinity, the density of the gel network structure decreased, the pore size decreased, the pore wall became thinner and the surface smoothed. When the pH dropped into the acidic range, there was a red shift of the hydroxyl group and the sulfate group bands, an enhancement of the hydrogen bonding interaction and a blue shift of the methyl band and the amide I band. At pH 3, the microscopic network structure of IP and IPH were dense and uniform with small pore size, rough surface and thick pore wall. These results showed that the binary mixed gels had better gel properties at pH 3. The results provided a theoretical basis for the preparation of IP and IPH gels, and also enabled a deeper understanding of the mechanism of interaction between polypeptides and carrageenan. © 2023 Society of Industrial Chemistry.
研究领域:
食品科学
·
材料科学
·
化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2025
·
IF 5.2
Qiao Li,Shou-Jun Lai,Tingting Deng,Siyu Zhang,Teng Niu,Hai-xiong Shi,Hongmei Mu
A novel Schiff-base fluorescent probe (SA) has here been designed for lysine detection. A condensation reaction between thiophene-2-carboxaldehyde and 1,4-succinohydrazide was then used. The SA showed a selective “turn-on” fluorescence response (425 nm) to lysine in a pH-stable dimethyl sulfoxide (DMSO)/H₂O system (3:2, pH = 7.2), thereby achieving a detection limit of 8.21 × 10 −8 M. Both experimental and computational studies revealed that the SA formed a flexible V-shaped cavity via intramolecular non-covalent interactions. An adaptive encapsulation of lysine through synergistic O-H···N/O-H···O hydrogen bonds and σ-π interactions could, thereby, form a stable 1:1 complex. The present study has developed a rational design strategy for dynamic molecular probes and advanced high-performance sensing platforms for biomolecules.
研究领域:
分析化学
·
生物传感
·
有机化学
·
生物医学工程
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Linzhi TAN,L.I. Lin,Fuyu CHU,Keqiang LAI,Yiqun HUANG
The impacts of the extent of pasteurization treatments (90 °C; 8 min, mimicked home-cooking – 25 min, mimicked commercial pasteurization) on the generation of two potential hazardous substances, N ε -carboxymethyllysine (CML) and N ε -carboxyethyllysine (CEL), in fish steaks were investigated from the initial pasteurization throughout the later storage and reheating. A more intense pasteurization treatment not only resulted in more CML/CEL generated in fish steaks during the pasteurization, but also promoted CML/CEL formation during subsequent storage (0 °C, 0–7 d). The mean formation rates of CML and CEL (zero-order reaction) in commercially pasteurized fish steaks were 145 % and 95 % faster, respectively, compared to home-cooked ones during storage. The reheated (75 °C, 10 min) fish steaks previously subjected to commercial pasteurization followed by 7 d of storage contained 335 % more CML and 120 % more CEL than the home-cooked ones, implying the necessity to reduce these potentially hazardous compounds in commercial precooked muscle foods.
研究领域:
食品科学
·
营养生物化学
·
食品安全
·
加工与贮藏技术
·
毒理学
✓ 已验证
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2022
·
IF 7.425
Wenting Liu,Yuting Wang,Dechang Xu,Huiyu Hu,Yilun Huang,Yuwei Liu,Shaoping Nie,Chang Li,Mingyong Xie
The purpose was to investigate the contents of heat-induced hazards by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in 44 commercial nuts. Results showed that content ranges of Acrylamide (AA), 5-hydroxymethylfurfural (5-HMF), N ε -carboxymethyl-lysine (CML), N ε -carboxyethyl-lysine (CEL), 3-Deoxyglucosone (3-DG), Glyoxal (GO), and Methylglyoxal (MGO) were ND-123.57 µg/kg, 0.57–213.42 mg/kg, 3.18–18.67 mg/kg, 3.98–57.85 mg/kg, 1.5–133.86 mg/kg, 0.45–1.59 mg/kg and 0.29–13.84 mg/kg, respectively. Sunflower seeds contained more heat-induced hazards followed by pistachios, cashews, almonds, walnuts and hazelnuts. The content of 5-HMF was positively correlated with the content of 3-DG. CML exhibited positive correlation with content of GO while no correlation between CEL and MGO. Higher levels of 3-DG and 5-HMF were observed in nuts produced with sugar and honey. Deep processing had a stronger promoting effect on CML and CEL formation. These data could provide a crucial guide for consumers to select nut products which might reduce heat-induced hazards intake.
研究领域:
食品科学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2012
·Vol. 4
·期 9
·2711-2714
·
IF 1.547
Yanli Zhou,Zhichong Yang,Maotian Xu
A simple, reliable and sensitive colorimetric method for the detection of lysine was developed based on the use of citrate-stabilized gold nanoparticles (AuNPs). In acid media (pH = 3.0), AuNPs exhibited a sensitive color change from red to blue after the addition of lysine for 30 min at room temperature, which could directly be seen with the naked eye and monitored by UV-vis absorption spectroscopy. Results showed that the absorbance ratio (A680/A520) was linear for a lysine concentration in the range of 1 to 70 μM (r = 0.995), and the limit of detection was 0.8 μM at a signal-to-noise ratio of 3. This method also demonstrated excellent selectivity for lysine over other amino acids. For the analysis of lysine in food samples, precision and accuracy were checked.
研究领域:
分析化学
·
生物化学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）