FOOD CHEMISTRY
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2025
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IF 9.8
Liang Luo,Zizhe Li,Qiang Li,Qiyuan Zhao,Xiaonan Wang,Ghulam Mujtaba Mari,Jianzhong Shen,Xuezhi Yu,Zhanhui Wang,Yantong Pan,Jijun Kang
Xylazine (XYL), an illicit opioid adulterant, its abuse and misuse can cause serious food safety incidents, drug-facilitated crimes, and even death. Therefore, to address the urgent need for XYL monitoring, a novel ratiometric fluorescence (RF) nanozyme-linked immunosorbent assay (NLISA) was proposed. This RF-NLISA used a robust polyvinylpyrrolidone ligand assisted trimetallic PtPdRu nanocluster (NC) nanozyme with high oxidase-like activity as the signal label. The PtPdRu NC could simultaneously catalyze the dual substrates scopoletin and Amplex Red to induce opposing fluorescence intensity changes, enabling sensitive RF signal output. Based on PtPdRu NC catalysis to trigger self-calibrating RF signals, the RF-NLISA achieved a limit detection of 0.0092 ng mL −1 . This represents a nearly 10-fold sensitivity improvement over traditional horseradish peroxidase-based colorimetric ELISA, while maintaining excellent specificity and accuracy. This novel PtPdRu NC nanozyme facilitated RF-NLISA provides a sensitive and accurate sensing platform for XYL abuse monitoring in various food and human urine samples
研究领域:
分析化学
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生物传感
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食品安全
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毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Environmental Technology & Innovation
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2025
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IF 7.1
Haixu Zhang,Sen Yang,Xilu Zhang,Yaxin Pei,Mengxiao Chen,Hongge Chen,Jiran Zhang
Cyromazine, a widely used insect growth regulator, accumulates in livestock manure and poses risks to beneficial insects such as black soldier fly larvae (BSFL) during waste bioconversion. This study isolated two bacterial strains capable of degrading cyromazine, Bacillus subtilis CYR1 (CYR1) and Bacillus velezensis CYR2 (CYR2), from contaminated chicken manure. Both strains demonstrated significant degradation efficiency via hydrolysis of cyromazine to cyanuric acid, a less toxic metabolite. However, in chicken manure systems, degradation efficiency reached a plateau at approximately 20%, likely due to preferential utilization of alternative nitrogen sources and inherent metabolic constraints. These findings highlight the potential of microbial remediation to mitigate cyromazine contamination while underscoring the need for strain optimization and comprehensive ecotoxicological assessments.
研究领域:
环境科学
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微生物学
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昆虫学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
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2022
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IF 9.231
Linlin Chen,Xiaofei Hu,Yaning Sun,Yunrui Xing,Gaiping Zhang
In this study, a novel hapten was designed and successfully synthesized to prepared the artificial immunogen against xylazine (XYL), then the monoclonal antibody 1H2-2C6 was obtained and applied to a lateral flow immunoassay (LFI) strip for rapid detection of XYL in milk. The visual detection limit (cutoff value) of the LFI strip was 5 ng/mL, the linear regression formula was Y = −0.521lg(X) + 0.382 ( R 2 = 0.995, n = 6), the linear detection range was 0.16–2.23 ng/mL and the detection limit (LOD) was 0.1 ng/mL. The recovery rates were in the range from 88.95% to 91.45%, the highest coefficient of variation was 8.89% (n = 3). The LFI strip was also validated by LC-MS/MS, as revealed, there were no significance differences between the two methods (the confidence interval was 95%). In brief, the LFI strip provides a reliable, sensitive and portable testing tool for screening of XYL on-site.
研究领域:
分析化学
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食品科学
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免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL LETTERS
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2023
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IF 2
Ge Lin Liu,Hao Zhe Hou,Yan Su,Yao Jia Peng,Yin Liang Wu,Jian Ping Wang
The residues of xylazine and its metabolite in foods of animal origin are dangerous to consumers, but there has been no enzyme-linked immunosorbent assay (ELISA) or ELISA-like method reported to determine them so far. In this study, molecularly imprinted microspheres with xylazine as the template were synthesized, and 2,6-dimethylaniline (the metabolite of xylazine) was used as a hapten to prepare a streptavidinated horseradish peroxidase-labeled conjugate. A direct competitive pseudo-ELISA was developed on a microplate for xylazine and 2,6-dimethylaniline in milk. Because biotinylated horseradish peroxidase was employed to enhance the signal, the limits of detection for xylazine (0.003 ng/mL) and 2,6-dimethylaniline (0.005 ng/mL) were increased by 16.7- and 14-fold, respectively, compared to a horseradish peroxidase-labeled conjugate. Moreover, this material may be reused three times. The analysis of milk samples was consistent with values obtained by liquid chromatography tandem mass spectrometry. This is the first paper reporting a molecularly imprinted polymer-based method for the determination of xylazine and its metabolites in food.
研究领域:
分析化学
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食品安全
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生物化学
✓ 已验证
产品关联
DOI
高影响因子
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