LWT-FOOD SCIENCE AND TECHNOLOGY
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2023
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IF 6
Yan Zhu,Yuanyuan Yao,Jinfeng Xi,Caiguo Tang,Lifang Wu
Fusarium graminearum ( F. graminearum ) is a vital pathogen of Fusarium head blight (FHB). Hydrogen sulfide (H 2 S), which can be induced by stress in plants as a fungal inhibitor, affects the germination of spores through the detection of environmental factors such as temperature and pH. Based on mathematical models, F. graminearum was treated with different temperatures, pH, and H 2 S concentration to investigate spore germination events. This paper considered the synergistic effects of temperature and pH on spores in order to determine the optimal germination environment. Under these circumstances, the inhibition of spore germination with different concentrations of H 2 S was quantified. The results suggested that the germination time negatively correlates with the germinating rate. An increase in temperature altered the weak alkali preference of F. graminearum spores. At 28 °C, spores were more sensitive to the changes in pH. H 2 S gas could delay the germinating process significantly, however the required H 2 S concentration varied with environmental changes. This paper provides a perspective on the synergistic effect of temperature and pH on spore germination and contributes to determining the concentration of H 2 S as a management of FHB.
研究领域:
植物病理学
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微生物学
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环境科学
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产品关联
DOI
高影响因子
Applied Nanoscience
·
2022
·Vol. 12
·期 9
·2683-2693
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IF 3.869
Chi Yu,Ye Zhenghe,Su Xianyan,Li Zhao,Ren Xuexiang
In this work, a compound was fabricated using β-cyclodextrin (β-CD) and propithiumazole (PTC). FTIR and TG were used to analyze the intermolecular force and thermal stability of the compound and results indicated. The intermolecular forces were van der Waals forces. According to previous research, the seed coat agent and suspension concentrate (SC) were prepared using this compound and auxiliary. The inhibitory effect on Fusarium graminearum was studied with a bioassay. The data suggested inhibition rate of microencapsulated PTC are about 7.8% and 5% higher than PTC at 72 h and 192 h, respectively. In addition, results of pot experiments suggested dry weight of crops were increased after using seed coat agent contained microencapsulated prothioconazole which indicates that the seed coat agent may be beneficial to protein synthesis. Compared to commercial PTC, field experiments were conducted to investigate the preventive effects of SC against fusarium head blight (FHB). The results of toxicity test and field experiment showed that this compound can improve the utilization efficiency about 5% of PTC and reduce pesticide injury to wheat seeds.
研究领域:
植物保护
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农药科学
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产品关联
DOI
高影响因子
CHEMBIOCHEM
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2024
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IF 2.6
Wen Fu,Zhongli Shao,Zhiping Xu,Zhong Li,Xusheng Shao
Graphical NP-TBZ is developed by covalently linking o -nitrobenzyl ( NP ) with thiabendazole ( TBZ ). Compound NP-TBZ can be controlled to release TBZ in dependent to light, which resulted in controllable in-vivo activity against wheat scab. It is promising for spatiotemporal controlled-release of TBZ in factual applications, posing a theoretical guidance in development of controlled-release pesticides. Pesticides are essential in agricultural development. Controlled-release pesticides have attracted great attentions. Base on a principle of spatiotemporal selectivity, we extended the photoremovable protective group (PRPG) into agrochemical agents to achieve controllable release of active ingredients. Herein, we obtained NP-TBZ by covalently linking o -nitrobenzyl ( NP ) with thiabendazole ( TBZ ). Compound NP-TBZ can be controlled to release TBZ in dependent to light. The irradiated and unirradiated NP-TBZ showed significant differences on fungicidal activities both in vitro and in vivo. In addition, the irradiated NP-TBZ displayed similar antifungal activities to the directly-used TBZ , indicating a factual applicability in controllable release of TBZ . Furthermore, we explored the action mode and microcosmic variations by SEM analysis, and demonstrated that the irradiated NP-TBZ retained a same action mode with TBZ against mycelia growth.
研究领域:
农业科学
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植物保护
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产品关联
DOI
PubMed
高影响因子
MOLECULES
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2024
·Vol. 29
·期 9
·1996
·
IF 4.2
Yilin Zheng,Yuqi Geng,Wenlong Hou,Zhe Li,Caihong Cheng,Xiuping Wang,Yuedong Yang
The wheat scab caused byFusarium graminearum(F. graminearum) has seriously affected the yield and quality of wheat in China. In this study, gallic acid (GA), a natural polyphenol, was used to synthesize three azole-modified gallic acid derivatives (AGAs1–3). The antifungal activity of GA and its derivatives againstF. graminearumwas studied through mycelial growth rate experiments and field efficacy experiments. The results of the mycelial growth rate test showed that the EC50of AGAs–2 was 0.49 mg/mL, and that of AGAs–3 was 0.42 mg/mL. The biological activity of AGAs–3 onF. graminearumis significantly better than that of GA. The results of field efficacy tests showed that AGAs–2 and AGAs–3 significantly reduced the incidence rate and disease index of wheat scab, and the control effect reached 68.86% and 72.11%, respectively. In addition, preliminary investigation was performed on the possible interaction between AGAs–3 andF. graminearumusing density functional theory (DFT). These results indicate that compound AGAs–3, because of its characteristic of imidazolium salts, has potential for use as a green and environmentally friendly plant-derived antifungal agent for plant pathogenic fungi.
研究领域:
植物保护
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分子生物学
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农业科学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Junfan Niu,Chao Wang,Ke Qiao,Min Liao,Ying Liu,Yi Ding,Huanhuan Yao,Huili Zhang,Haiqun Cao
Poor foliar deposition and retention of pesticides results in serious pesticide residues and environmental pollution. Organic-inorganic hybridized nanoparticles (OIHN), combining the advantages of organic and inorganic materials, can be used as carriers to load pesticides for efficient and safe application. Herein, a novel multifunctional OIHN composed of mesoporous silica nanoparticles (MSNs) and cationic chitosan quaternary ammonium salt (HACC) was constructed and used as a delivery system for prothioconazole (PTC). The resultant PTC@MSNs-HACC exhibited a remarkable loading capacity of 39.07 wt% and demonstrated enhanced PTC release (31.47 %) under alkaline conditions. The UV-shielding properties of MSNs efficiently shielded PTC from photodegradation, increasing its photostability by over threefold. The strong positive charge of HACC conferred excellent adhesion of PTC@MSNs-HACC to fungal cell membranes, leading to high deposition on wheat leaves with improved rain-wash resistance (increased by 30 %). Consequently, PTC@MSNs-HACC (EC 50 : 12.48 mg/L) exhibited superior wheat scab control compared to PTC emulsifiable concentrate (EC 50 : 28.49 mg/L). Additionally, PTC@MSNs-HACC displayed excellent uptake and transport in plants, ensuring plant safety and reducing toxicity to zebrafish by >1-fold. The potential application of the developed PTC@MSNs-HACC in agricultural production holds significant promise and is anticipated to find widespread use in the future.
研究领域:
农业科学
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植物保护
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材料科学
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2021
·
IF 5.279
Chuanyu Han,Zhiyin Yu,Yuting Zhang,Zhiyan Wang,Junwei Zhao,Sheng-Xiong Huang,Zhonghua Ma,Ziyue Wen,Chongxi Liu,Wensheng Xiang
In this study, the supernatant extract from fermentation broth of Streptomyces sp. NEAU-H3 showed strong antifungal activity against Fusarium graminearum strain PH-1 in vitro and in vivo. Three known pyranonaphthoquinones were isolated by means of an activity-guided method, and frenolicin B was characterized as the main active ingredient. Frenolicin B displayed strong antifungal activity against F. graminearum strain PH-1 with an EC50 value of 0.51 mg/L, which is lower than that of carbendazim (0.78 mg/L) but higher than that of phenamacril (0.18 mg/L). Frenolicin B could also strongly inhibit the mycelial growth of Fusarium species, including F. graminearum and F. asiaticum, as well as carbendazim-resistant Fusarium strains isolated from field, with EC50 values of 0.25–0.92 mg/L. Results from field experiments showed that the efficacy of frenolicin B in controlling Fusarium head blight at a treatment concentration of 75 g ai/ha was better than those of phenamacril (375 g ai/ha) and carbendazim (600 g ai/ha) or had no significant difference with that of phenamacril (375 g ai/ha) in 2 years. Scanning electron microscope and transmission electron microscope observations revealed that after treating F. graminearum mycelia with frenolicin B, the mycelia appeared aberrant and had an uneven thickness and swelling, the cytoplasm had disintegrated, and some cell contents were lost. Transcriptome analysis suggests that frenolicin B might inhibit the metabolism of nucleotides and energy by affecting genes involved in phosphorus utilization but did not affect the expression of myosin 5, which is the specific target of phenamacril. These findings indicate that frenolicin B may be a potential agrochemical fungicide for controlling Fusarium head blight.
研究领域:
农业
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植物病理学
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微生物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2021
·
IF 3.963
Chao Xu,Hongzhou Chen,Qinyan Wu,Yuqi Wu,Paul Daly,Jian Chen,Hongfu Yang,Lihui Wei,Yiqing Zhuang
Fusarium head blight( FHB )caused by Fusarium graminearum species complex (FGSC) is one of the most important diseases around the world. Deoxynivalenol (DON) is a type of mycotoxin produced by FGSC when infecting cereal crops . It is a serious threat to the health of both humans and livestock. Trehalose-6-phosphate phosphatase ( TPP ), a conserved metabolic enzyme found in many plants and pathogens, catalyzes the formation of trehalose. N-(phenylthio) phthalimide (NPP) has been reported to inhibit the normal growth of nematodes by inhibiting the activity of TPP , but this inhibitor of nematodes has not previously been tested against F. graminearum . In this study, we found that TPP in F. graminearum (Fg TPP ) had similar secondary structures and conserved cysteine (Cys356) to nematodes by means of bioinformatics. At the same time, the sensitivity of F. graminearum strains to NPP was determined. NPP exhibited a better inhibitory effect on conidia germination than mycelial growth . In addition, the effects of NPP on DON biosynthesis and trehalose biosynthesis pathway in PH-1 were also determined. We found that NPP decreased DON production, trehalose content, glucose content and TPP enzyme activity but increased trehalose-6-phosphate content and trehalose-6-phosphate synthase ( TPS ) enzyme activity. Moreover, the expression of TR I 1 , TR I 4 , TR I 5 , TR I 6 , and TPP genes were downregulated, on the contrary, the TPS gene was upregulated. Finally, in order to further determine the control ability of NPP on DON production in the field, we conducted a series of field experiments, and found that NPP could effectively reduce the DON content in wheat grain and had a general control effect on FHB. In conclusion, the research in this study will provide important theoretical basis for controlling FHB caused by F. graminearum and reducing DON production in the field.
研究领域:
植物病理学
·
真菌学
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农学
·
毒理学
✓ 已验证
DOI
PubMed
高影响因子
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