PEST MANAGEMENT SCIENCE
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2022
·Vol. 78
·期 11
·4497-4506
·
IF 4.462
Yuxin Zhang,Qinghao Liu,Wangcang Su,Lanlan Sun,Hongle Xu,Fei Xue,Chuantao Lu,Renhai Wu
BACKGROUND S -metolachlor (MET) was used to prevent weed infestation in sorghum fields, but inappropriate application could result in phytotoxicity on sorghum. Exogenous gibberellin A 3 (GA 3 ) has been applied for alleviating the phytotoxicity of MET. However, its detoxification mechanism is still not well known. RESULTS Leaf deformity of sorghum caused by 200 mg/L MET was alleviated by treating sorghum shoots with 800 mg/L GA 3 , and the injury recovery rate of growth index was over 73%. More importantly, GA 3 could not accelerate the metabolic rate of MET in sorghum. The result of phytohormone metabolomics showed that endogenous GA 3 content in sorghum decreased by 78.10% with MET treatment, while abscisic acid (ABA) content increased by 120.2%, resulting in 10.3-fold increase of ABA/GA 3 ratio. Content of ABA and GA 3 increased by 11.9- and 21.1-fold with MET and GA 3 treatment, respectively, leading to ABA/GA 3 ratio restoration. Moreover, MET inhibited the expression of genes encoding key enzymes related to GA synthesis including CPS1 , KO2 , KAO , GA20ox1D and ABA8ox gene related to ABA metabolism. The transcription levels of GA metabolism-related genes CYP714D1 and GA2ox were up-regulated by 11.2- and 7.2-fold, while ABA synthesis-related genes NCED and ZEP were up-regulated by 8.0- and 3.0-fold, respectively, with MET and GA 3 treatment. Conclusion In this study, exogenous GA 3 protecting sorghum shoots from MET phytotoxicity was due to supplement the MET-induced GA 3 deficiency by absorbing exogenous GA 3 , and restore homeostasis of ABA and GA 3 by promoting ABA synthesis, which provides novel insights for mechanism of GA 3 alleviating MET phytotoxicity. © 2022 Society of Chemical Industry.
研究领域:
植物生理学
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植物激素研究
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农学
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产品关联
DOI
PubMed
高影响因子
Metabolites
·
2024
·Vol. 14
·期 2
·96
·
IF 3.4
Chen Zeng,Wen-Jing Cai,Liu-Cheng Jiang,Tiantian Ye,Yu-Qi Feng
Gibberellins (GAs) play a pivotal role in modulating plant growth and development. Glucose–conjugated gibberellins (Glc–GAs), a prevalent conjugated form of GAs, regulate intracellular GA levels by the coupling and decoupling of glucose groups. However, the diversity of Glc–GAs identified within individual species remains limited, hinting at a multitude of yet undiscovered gibberellin metabolites. This lacuna poses considerable impediments to research efforts dedicated to comprehensively delineating the GA metabolic pathway. In this study, we developed a structure–oriented screening and identification method for Glc–GAs in plant species by employing LC–MS/MS coupled with chemical derivatization. Through the application of chemical derivatization technique, carboxyl groups on Glc–GAs were labeled which effectively enhanced the sensitivity and selectivity of mass spectrometry detection for these compounds. Concurrently, the integration of mass spectrometry fragmentation and chromatographic retention behavior facilitated the efficient screening and identification of potential Glc–GAs. With this strategy, we screened and identified 12 potential Glc–GAs from six plant species. These findings expand the Glc–GA diversity in plants and contribute to understanding GA metabolic pathways.
研究领域:
植物生理学
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分析化学
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植物化学
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产品关联
DOI
PubMed
高影响因子
ACTA PHYSIOLOGIAE PLANTARUM
·
2016
·Vol. 39
·期 1
·1-14
·
IF 1.563
Wang Yang,Ren Tao,Lu Jianwei,Cong Rihuan,Hou Wenfeng,Liu Tao,Hussain Saddam,Li Xiaokun
Inferior tillers typically provide a minimal contribution to the grain yields of rice; however, with the proper regulation of light and nutrients, their yields may be significantly enhanced. The exogenous introduction of gibberellic acid (GA 3 ) may assist with extending the culm lengths of inferior tillers, the sequestration of additional sunlight, and the accumulation of further nutrients. In an attempt to verify our assumption while enhancing the grain yield of inferior tillers, GA 3 was applied to inferior tillers under different nitrogen (N) levels. Morphological alterations, photosynthetic parameters, endogenous GA content, N accumulation, and soluble sugars within the leaves of inferior tillers were assessed, as was the activity of sucrose synthase (SuSase) in the cleavage direction. Our results revealed that the belated emergence and low concentration of endogenous GA 1+3 translated to the stunted growth of inferior tillers, which ultimately had a reduced capacity to harvest sunlight. Furthermore, insufficient sunlight due to asymmetric competition might be the source of the reduction of inferior tiller source and sink activities. Foliar GA 3 spray enhanced the intercepted photosynthetically active radiation (IPAR) and SuSase activity in inferior tillers considerably; however, dry matter and N accumulation provided adequate resources to grains only when the tillers obtained sufficient N. The alterations in soluble sugars indicated that inferior tillers did not respond well to the application of GA 3 under an insufficient N supply. In contrast, when sufficient N was made available, GA 3 -treated inferior tillers received high-intensity light, and additional energy was allocated to the photochemical reactions of photosystem II (PSII). The high quantum efficiency of PSII contributed to the transportation of C- and N-assimilates from the source organ to the sink under adequate N conditions, and eventually increased the grain yields of the inferior tillers.
研究领域:
植物生理学
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农学
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产品关联
DOI
高影响因子
ANNALS OF BOTANY
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2024
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IF 3.6
Guo Huanhuan,Li Shiqin,Liu Yong,Yang Qinsong
Background and AimsChinese pistachio (Pistacia chinensis Bunge), an important horticultural plant species, holds great ornamental value with beautiful leaves and fruits. Seedling propagation of this tree species is restricted by its erratic seed germination, however, the germination mechanism is ambiguous yet. The aim of this study was to figure out the germination mechanism from the novel perspective based on the multi-omics data.MethodsThe multi-omics technique combined with hormone content measurement was first applied in seed germination of Chinese pistachio.Key ResultsDue to the great accumulation during seed germination, catechin stood out from the identified metabolites by broadly targeted metabolomic analysis. Exogenous catechin of 10 mg/L significantly improved the germination of Chinese pistachio seeds. An interesting result of hormone analysis showed that the improving effect of catechin could be attributed to increase of the gibberellic acid 3 (GA3) content rather than decrease of the abscisic acid (ABA) content before germination. The paclobutrazol (PAC, a GA biosynthesis inhibitor) and PAC + catechin treatments also showed that the promoting effect of catechin on seed germination depends on GA biosynthesis. Transcriptome analysis and qRT‒PCR further revealed that catechin induced the expression of PcGA20ox5 to activate GA biosynthesis. Several transcription factors were induced by catechin and GA treatments, such as TCP, bZIP and C3H, which may play an important regulatory role in GA biosynthesis in a catechin-mediated way.ConclusionsCatechin promotes seed germination via GA biosynthesis in Chinese pistachios. This study proposes a novel mechanism by which catechin promotes seed germination via the GA pathway, which provides new insight into a comprehensive understanding of seed dormancy and germination.
研究领域:
植物学
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植物生理学
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分子生物学
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园艺学
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产品关联
DOI
PubMed
高影响因子
3 Biotech
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2020
·Vol. 10
·期 7
·1-10
·
IF 1.798
Peng Xiao-Lun,Zhao Wei-Jun,Wang Yuan-Shan,Dai Ke-Lei,Cen Yu-Ke,Liu Zhi-Qiang,Zheng Yu-Guo
Gibberellic acid (GA 3 ) is a natural plant growth hormone that has been widely used in agriculture and horticulture. To obtain higher GA 3 producing strains, the method of screening the strains for resistance to simvastatin was used after treatment with nitrosoguanidine (NTG) and gamma rays. The rationale for the strategy was that mutants showing simvastatin resistance were likely to be high GA 3 producers, as their activity of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase is relatively more effective. GA 3 yield of mutant S109 increased by 14.2% than that of the original strain. The GA 3 production ability in mutant S109 remained relatively stable after ten generations. With the addition of 0.4 g glycerol on the 5th day during the fermentation process in Erlenmeyer flask, maximum GA 3 production of 2.7 g/L was achieved by this mutant, exhibiting 28.6% increase compared with original strain. Furthermore, we also achieved 2.8 g/L GA 3 and had a 33.3% increase with addition 20 g glycerol on the 5th day during the fermentation process in a 5-L bioreactor. Our results indicated efficient GA 3 production could be achieved on the condition that the supply of glycerol at the suitable conditions. This study would lay a foundation for industrial production of GA 3 .
研究领域:
植物学
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微生物学
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遗传学
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DOI
PubMed
高影响因子
PLoS One
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2022
·Vol. 17
·期 2
·e0263530
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IF 3.752
Shunde Su,Tengfei Zhu,Jun Su,Jian Li,Qing Zhao,Xiangyang Kang,Renhua Zheng
Flooding is one of the main abiotic stresses suffered by plants. Plants respond to flooding stress through regulating their morphological structure, endogenous hormone biosynthesis, and genetic signaling transduction. We previously found that Fokienia hodginsii varieties originating from Gutian exhibited typical flooding tolerance traits compared to three other provenances (Yongzhou, Sanming, Nanping), expressed as increased height, longer diameter at breast height (DBH), and smaller branch angle. Herein, the changes in endogenous gibberellins (GA) and abscisic acid (ABA) contents were measured under flooding stress in F . hodginsii , and ABA was found to decrease, whereas GA increased with time. Furthermore, the GA and ABA contents of the varieties originating from Gutian and the three other provenances were measured, and the results indicated that F . hodginsii from Gutian could respond more rapidly to flooding stress. The transcriptomes of the varieties originating from Gutian and the other three provenances were compared using RNA sequencing to explore the underlying genetic mechanisms of the flood-resistant phenotypes in F . hodginsii . The results indicated that two flood-stress response genes (TRINITY_DN142_c0_g2 and TRINITY_DN7657_c0_g1) were highly related to both the ABA and GA response in F . hodginsii .
研究领域:
植物学
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分子生物学
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遗传学
✓ 已验证
DOI
PubMed
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
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2016
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IF 2.463
Xin-Jun Yu,Jie Sun,Ya-Qi Sun,Jian-Yong Zheng,Zhao Wang
In this study, the phytohormone gibberellin was evaluated for its effect on lipid and docosahexaenoic acid (DHA) accumulation in Aurantiochytrium sp.YLH70. It was found that 4 mg/mL of gibberellin increased biomass, lipid and DHA yield of Aurantiochytrium sp.YLH70 by 14.4%, 43.6% and 79.1%, respectively. Moreover, a GC–MS metabolomics method combined with a multivariate analysis was applied to reveal the metabolic mechanisms responsible for the increased lipid and DHA accumulation. The principal component analysis (PCA) revealed that metabolomics profiles from all groups were discriminated. 38 metabolites identified by the partial least-squares-discriminant analysis (PLS-DA) were responsible for responding to gibberellin treatment. Metabolic pathway analysis showed that gibberellin accelerated the rate of utilization of glucose, and metabolites in fatty acids biosynthesis and mevalonate pathway were increased, while metabolites in glycolysis and TCA cycle were decreased in Aurantiochytrium sp.YLH70. Moreover, the anti-stress mechanism in Aurantiochytrium sp.YLH70 might be induced by gibberellin.
研究领域:
生物化学
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代谢组学
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DOI
高影响因子
Food Chemistry-X
·
2025
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IF 8.2
Haozhen Li,Shuyao Wang,Xiaohua Zhang,Kangkang Song,Long Yang
Tea quality in greenhouse was certain gap with open air. Metabolites and foliar microorganisms were investigated under seaweed fertiliser (CF) and gibberellin (CH) treatments using sensory evaluation, HPLC, untargeted metabolomics, 16S rDNA, and Internal Transcribed Spacer. CF tea was mellow, less astringent, and of better quality compared to CH. Catechin, −(−)Epicatechin, and Epigallocatechin were notably lower in CF. Differentially accumulated metabolites (DAMs) were notably enriched in Flavonoid and Phenylpropanoid biosynthesis, both involved in Catechin synthesis. DAMs in these pathways appeared down-regulated in CF. The CF improved quality by down-regulating metabolites in Phenylpropanoid biosynthesis in conjunction with microbial community metabolism enriched in amino acid and secondary metabolite biosynthesis. Metabolite- microbial correlation analysis indicated that the highest correlation with phenylpropane pathway metabolites was in bacteria Variovorax and Pseudomonas , and in fungi Filobasidium . The study provides theoretical basis for regulating flavour quality of greenhouse tea.
研究领域:
植物学
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代谢组学
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微生物学
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产品关联
DOI
PubMed
高影响因子
Plants-Basel
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2024
·Vol. 13
·期 4
·543
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IF 4
Long Lu,Xinyu Chen,Qinyan Tan,Wenqian Li,Yanyan Sun,Zaoli Zhang,Yuanyuan Song,Rensen Zeng
Aluminum toxicity poses a significant constraint on crop production in acidic soils. While phytohormones are recognized for their pivotal role in mediating plant responses to aluminum stress, the specific involvement of gibberellin (GA) in regulating aluminum tolerance remains unexplored. In this study, we demonstrate that external GA exacerbates the inhibitory impact of aluminum stress on root growth of rice seedlings, concurrently promoting reactive oxygen species (ROS) accumulation. Furthermore, rice plants overexpressing the GA synthesis geneSD1exhibit enhanced sensitivity to aluminum stress. In contrast, theslr1gain-of-function mutant, characterized by impeded GA signaling, displays enhanced tolerance to aluminum stress, suggesting the negative regulatory role of GA in rice resistance to aluminum-induced toxicity. We also reveal that GA application suppresses the expression of crucial aluminum tolerance genes in rice, includingAl resistance transcription factor 1(ART1),Nramp aluminum transporter 1(OsNramp4), andSensitive to Aluminum 1(SAL1). Conversely, theslr1mutant exhibits up-regulated expression of these genes compared to the wild type. In summary, our results shed light on the inhibitory effect of GA in rice resistance to aluminum stress, contributing to a theoretical foundation for unraveling the intricate mechanisms of plant hormones in regulating aluminum tolerance.
研究领域:
植物学
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遗传学
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植物生理学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF RARE EARTHS
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2019
·
IF 2.846
Cansong Lu,Yanjun Ma,Jianwen Wang
Rare earth elements (REEs) have been widely applied as efficient elicitors to improve production of biologically important secondary metabolites in plants. However, their effects on microbial secondary metabolites were seldom reported. In this study, lanthanum (LaCl 3 ) was used as an elicitor in mycelium cultures of Shiraia bambusicola to stimulate the production of hypocrellin A (HA), a promising photosensitizer for anticancer photodynamic therapy. La 3+ at 1.0 g/L not only increases HA content by 2.50-fold in mycelia, but stimulates HA exudation to the medium with the highest production (225.05 mg/L) on day 6. Further analysis shows that La 3+ can induce the intracellular ROS (reactive oxygen species) accumulation with 2.33-fold higher contents of H 2 O 2 in mycelium. HA production induced by La 3+ treatment can be suppressed by ROS scavengers. Further analysis reveals that the elicitation of La 3+ on HA accumulation is mediated by ROS generation to enhance expression of HA biosynthetical genes. The expression of ATP-binding cassette transporter gene ( SbABC ) for HA exudation is also regulated partially via ROS generated by NADPH oxidase . These results indicate that the enhanced HA production by La 3+ might be mediated by ROS signaling pathway. Our results provide a basis for understanding REE elicitation on fungal metabolites and a practical biotechnological process for enhanced production of HA.
研究领域:
微生物学
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生物技术
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产品关联
DOI
高影响因子
NEW ZEALAND JOURNAL OF CROP AND HORTICULTURAL SCIENCE
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2022
·
IF 1.094
Yu Zhao,Sicen Liu,Bing He,Min Sun,Jishou Li,Rong Peng,Liangliang Sun,Xiaopeng Wang,Youpeng Cai,Hehe Wang,Xueqing Geng
Microorganisms in the plant's rhizosphere are capable of solubilising insoluble soil phosphates and can promote plant growth. In this study, a total of 30 bacteria were isolated from soils in Changji city, and one highly efficient strain (B5) was identified as a member of Pseudomonadaceae. B5 exhibited phosphorus (P) solubilisation of 519.66 mg/L in a culture medium containing tricalcium phosphate (Ca3 (PO4)2). Results showed that strain B5 had a high P utilisation rate in a medium containing glucose (monosaccharide), ammonium sulphate and tricalcium phosphate. B5 treatment significantly promoted plant growth and increased the dry weight, the chlorophyll content as well as P content of tomato, marigold and cucumber. Moreover, B5 produced plant growth-promoting hormones, including indole-3-acetic acid (IAA) (4.18 g/L) and gibberellin (GA) (19.8 mg/L). In addition, organic acids including oxalic acid, succinic acid, gluconic acid, arabic-hexanoic acid and glutraic acid were produced by B5 under different phosphorus sources. Our results indicated that B5 is a potential candidate for development as a bioinoculant for horticultural application.
研究领域:
微生物学
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园艺学
✓ 已验证
DOI
高影响因子
JOURNAL OF PLANT PHYSIOLOGY
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2025
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IF 4.1
Mi Qi-Li,Lv Hong-Tao,Huang Fei-Fei,Xu Li,Huang Zi-Ying,Yang Wen-Wu,Zou Mei-Yun,Huang Hai-Tao,Zeng Wan-Li,Zhao Jia-Yin,Chen Qing-Xian,Si-Tu Yong-En,Xiang Hai-Ying,Jiang Yuan-Qi,Mai Zhi-Tong,Ding Shu-Yuan,Liu Zhan-Yu,Hu Bo-Keng,Li Liu-Hong,Li Xue-Mei,Yu Min,Wu Fei-Hua,Gao Qian
Nitrogen is a critical nutrient for plant growth and development. While numerous studies have investigated the mechanisms by which nitrate and/or ammonium regulate plant growth, little is known about whether and how amino acids regulate plant leaf development. This study demonstrates that tobacco plants with altered expression levels of an amino acid transporter (LYSINE HISTIDINE TRANSPORTER1, NtLHT1 , Ntab0818090) exhibit significant differences in leaf morphology. Knock-out mutants exhibit elongated and narrower leaves compared to wild-type plants, whereas overexpression (OE) lines display orbicular leaves. Additionally, mutant plants exhibit decreased nitrogen use efficiency (NUE) under half MS medium and delayed development under nitrogen-depleted conditions. Moreover, overexpression lines demonstrate better performance. Although the mutant does not show significant lower level of nitrate or total amino acid content in the developing leaves, its amino acid profile, particularly glutamine (Gln), is significantly altered. Supplementation with Gln in the growth medium, rather than glutamate, can restore the morphological differences observed in mutant leaves, suggesting a pivotal role of Gln in regulating leaf shape. To further elucidate the mechanisms underlying leaf shape regulation, we analyzed endogenous hormone levels and applied exogenous hormones to these lines. Our findings suggest that amino acids transported from source organs, particularly glutamine (Gln), play a key role in controlling leaf development and morphology through the modulation of multiple phytohormones, such as gibberellic acid (GA).
研究领域:
植物学
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遗传学
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分子生物学
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营养生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）