FOOD CONTROL
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2014
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IF 2.819
Chunmei Jiang,Junling Shi,Xianqing Chen,Yanlin Liu
Fungal profiles and ochratoxin A (OTA) accumulation during wine making were investigated using five different wine grape cultivars, Cabernet Sauvignon, Pinot Noir, Merlot, Syrah and Petit Verdot and the intrinsic influences caused by sulfur dioxide, ethanol and combine effect of ethanol and reducing sugar were analyzed using Cabernet Sauvignon and inoculation of Aspergillus carbonarius . Aspergillus spp. and Penicillium spp. were found as the major fungi in all winemaking processes and were highly correlated with OTA accumulation in wine. Most fungi died and OTA production decreased after 48 h of alcoholic fermentation , being consistent with the period when ethanol accumulation increased. The addition of SO 2 significantly inhibited the growth and OTA production of A. carbonarius with complete inhibition at 500 mg/L. When the ethanol concentration in the must increased to the range of 2–4%, growth and OTA production of A. carbonarius were significantly inhibited. Reducing sugar concentration had no significant effect on the growth and OTA production of A. carbonarius within the levels changing during the winemaking. Therefore, the increase of ethanol concentration played an important role in causing the decrease of fungal contamination and OTA accumulation during winemaking.
研究领域:
食品微生物学
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真菌毒素学
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酿酒科学
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食品安全
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应用微生物学
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分子毒理学
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高影响因子
ANALYTICA CHIMICA ACTA
·
2026
·
IF 6.1
Xinying Wang,Yujie Chang,Yiyan Zhang,Xuan Yu,Yixuan Zou,Zheqi Liu,Fuchuan Qi,Jingjing Ming
Accurate and rapid determination of aflatoxin M1 (AFM1) in food is crucial for ensuring the safety of the human diet. In view of this, we proposed a proximity-inducible Cas13a activity and hydrogel-assisted paper-based distance sensor for the detection of aflatoxin M1. The single-stranded DNA (ssDNA) and AFM1 aptamer were split into two parts and linked to the terminus of two separate DNA activator strands, respectively. By introducing AFM1, it stimulated these two DNA activator strands to assemble with CRISPR RNA (crRNA) through spatial proximity mechanism between the target and these two DNA activators to activate the Cas13a trans-cleavage towards the RNA-containing site within hairpin probe to release a DNA initiator. The released DNA initiator triggered the catalytic hairpin assembly (CHA) between magnetic beads (MBs)-DNA capture probe and trypsin-DNA detection probe. After hydrolysis of gelatin hydrogel by trypsin, water released from the hydrogel flowed along the pH indicator strip and exhibited a distinct mark. Depending on this portable method, the limit of detection (LOD) was achieved at 23.6 ng L -1 . Furthermore, our developed strategy can be applied to measure AFM1 in ultra-high temperature (UHT) milk with satisfactory effects, and its excellent specificity and reproducibility also demonstrate that our platform has great potential for application in environmental monitoring.
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ANALYTICAL CHEMISTRY
·
2026
·Vol. 98
·期 31
·23179-23189
·
IF 7.3
Lv Xianfeng,Chen Minyan,Lin Bangfeng,Jiang Yaru,Zhou Jicheng,Zheng Xinyi,Wu Wenxin,Li Ning,Zhuang Junyang
Although photoelectrochemical (PEC) sensing offers low
background
and high sensitivity, most PEC platforms rely on semiconductor materials
and immobilized recognition probes, which complicate electrode fabrication
and reduce operational simplicity. Herein, we report a fossilization-inspired
photoelectrochemical sensing platform (FIPSP) for ultrasensitive detection
of aflatoxin B1 (AFB1) based on DNA-induced in situ CaCO 3 crystal growth on a gold electrode (AuE). We found that nucleic
acids could induce interfacial biomineralization of calcite-like CaCO 3 crystals on AuE, and the resulting mineralized layer effectively
modulated the intrinsic anodic PEC response of AuE. In this design,
a bead-immobilized initiator DNA was first hybridized with an AFB1
aptamer. Upon target recognition, the aptamer dissociated from the
bead-immobilized initiator DNA, allowing the initiator to trigger
a hybridization chain reaction (HCR) between two hairpin DNA probes.
After alkaline treatment, the HCR products were disassembled into
DNA strands, which were then released and adsorbed onto AuE to induce
in situ CaCO 3 crystal growth. The mineralized layer hindered
interfacial charge transfer and ascorbic acid diffusion, thereby suppressing
the photocurrent and enabling detection of AFB1. Under optimized conditions,
the developed FIPSP exhibited a linear range from 1 ag mL –1 to 10 pg mL –1 and a detection limit of 0.61 ag
mL –1 , together with excellent selectivity and reproducibility.
It also showed satisfactory performance in soybean oil and maize samples.
This work establishes a simplified PEC electrode construction strategy
that requires neither semiconductor photoactive material synthesis
nor probe immobilization on the electrode surface, providing a promising
platform for ultrasensitive AFB1 analysis.
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高影响因子
MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 7
·503
·
IF 5.4
Xiao Yue,Wen Xuefei,Zhang Xingzhong,Chen Hongrui,Tang Jie,Chi Hai,Li Liman,Thanapop Soteyome
To enable sensitive quantitative detection of zearalenone (ZEN) in cereal matrices, this study develops a molecularly imprinted electrochemical sensor. A three-dimensional flower-shaped carbon composite material (CoNiZn@C), synthesized from a ternary CoNiZn metal–organic framework, was utilized as a highly conductive substrate featuring excellent electrical conductivity and abundant binding sites. Density functional theory calculations were conducted to elucidate the intermolecular interactions and cooperative recognition mechanisms between zearalenone and the selected monomers. Experimentally, the AuNPs-based thioaniline units (PATP@AuNPs) and pyrrole were employed as bifunctional monomers. Specifically, PATP@AuNPs can be stably immobilized on the substrate surface to enhance interfacial electron transfer efficiency, while simultaneous in situ copolymerization of the two monomers generates numerous non-covalent binding cavities capable of specifically recognizing ZEN. Under optimized experimental conditions, the sensor exhibited a linear detection range from 10 nM to 1000 µM and a detection limit as low as 1.6 nM. This detection approach is straightforward, cost-effective, and demonstrates excellent specificity and sensitivity, indicating substantial potential for rapid screening and quantitative analysis of mycotoxins in agricultural products. Graphical
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Frontiers in Veterinary Science
·
2026
·
IF 3.1
Lin-Lin Hu,Yang Yi,Xing-He Ke,Min-Min Ou,Lan-Lan Nong,Li-Zhou Qin,Shao-Chen Sun,Caizhu Wang,Bi-Yun Liao
IntroductionOchratoxin A is a toxic mycotoxin produced by fungi, which is commonly detected in contaminated cereals and animal feeds. It exhibits nephrotoxicity, hepatotoxicity, genotoxicity, immunotoxicity, and reproductive toxicity.MethodsIn the present study, we investigated the adverse effects of ochratoxin A on organelle distribution using a porcine oocyte model.ResultsWe demonstrated that in vitro exposure to ochratoxin A inhibited cumulus cell expansion and polar body extrusion in oocytes. Ochratoxin A reduced cortical endoplasmic reticulum distribution and increased GRP78 expression, indicating the induction of endoplasmic reticulum stress. In contrast, the Golgi apparatus exhibited abnormal accumulation, accompanied by decreased Rab7 expression and aberrant localization, suggesting impaired vesicular transport. Similarly, lysosomes accumulated in the cytoplasm of porcine oocytes following ochratoxin A treatment. We also found that mitochondrial function was disrupted, as evidenced by reduced ATP production and aberrant mitochondrial membrane potential.ConclusionTaken together, these results indicate that ochratoxin A impairs organelle distribution, induces endoplasmic reticulum stress, and causes vesicular transport defects during porcine oocyte maturation.
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Food Bioscience
·
2026
·
IF 6.2
Lihua Fan,Mingying Hu,Zongyuan Ou,Fan Li,Yiheng Shi,Di Wu,Yongning Wu,Guoliang Li
Sensitive, rapid, and reliable detection of aflatoxin B1 (AFB1) in food is of great importance for ensuring food safety. In this study, a dual-mode paper-based biosensing platform was developed by integrating the CRISPR/Cas12a nucleic acid cleavage system with a nanozyme-responsive DNA hydrogel for highly sensitive and portable detection of AFB1. Upon the presence of the target, AFB1 binds specifically to the aptamer, thereby activating the CRISPR/Cas12a system and subsequently inducing the degradation of the hydrogel network, which leads to the release of the encapsulated Au@Pt nanozyme. The released nanozyme catalyzes the oxidation of TMB in the presence of H 2 O 2 to generate a blue-colored product, while simultaneously producing a quantifiable diffusion signal on the paper-based substrate, enabling dual-mode colorimetric and distance-based detection. The detection limit of naked-eye detection is 0.1 ng mL −1 , and it is 0.075 ng mL −1 in the distance detection mode. The proposed method can complete the signal output within 30 min and exhibits the advantages of operational simplicity, rapid response, and high sensitivity. In practical peanut samples, satisfactory recoveries and good stability were achieved, demonstrating its potential as a promising strategy for on-site detection of trace mycotoxins in food.
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高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Wenyan Yan,Shuyue Li,Baoqing Bai,Ying Zhang,Jinhua Zhang,Tao Bo,Qinghua Wang,Ying Gu,Yukun Yang,Shuo Wang
A MIP-modified MOF/COF hybrid nanoplatform for PEC sensing, adsorption, and photocatalytic degradation was developed. • The MOF/COF hybrid enhanced photocurrent through improved light absorption and charge separation. • The MIP layer provided high selectivity. • The platform enabled ultrasensitive PAT detection and efficient adsorption-photocatalytic degradation. Rational design of photoactive materials is essential for the sensitive detection and efficient removal of hazardous contaminants. Herein, a visible-light-responsive covalent organic framework/metal-organic framework (COF/MOF) hybrid with uniform COF layers grown in-situ on the MOF surface, followed by the introduction of a molecularly imprinted polymer (MIP) layer via a sol-gel process, was constructed for the PEC sensing, adsorption, and photocatalytic degradation of patulin. The hybrid architecture enhanced photoelectrochemical performance by facilitating charge separation and improving light harvesting. The resulting PEC sensor exhibited a wide linear range (0.1 nM-1 mM) and a low detection limit (0.055 nM), along with good reproducibility and stability in real samples. Moreover, the MIP/MOF/COF hybrid enabled efficient adsorption and photocatalytic degradation of patulin, driven by photogenerated holes and superoxide radicals. This work provides a promising strategy for the efficient and highly sensitive detection and removal of contaminants. Download: Download high-res image (236KB) Download: Download full-size image
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Advanced Science
·
2025
·
IF 14.1
Tianci Xie,Changjiang Li,Minghao Hu,Xingyu Zhong,Junbin Xiao,Zhen Zhang,Ze Wang,Tongbo Wu
The capacity to retain and precisely release historical data at the right moments is typically managed by sequential logic circuits within computer systems. However, the reusability and autonomy of DNA sequential logic circuits still need to be developed. To bridge this gap, a series of sequential logic circuits are implemented by constructing a DNA strand replacement system regulated by a nicking enzyme (nickase). This nickase-integrated system dynamically resolves the thermodynamic-kinetic conflict, offering spatiotemporal control over strand displacement. These circuits include Set-Reset latches (SR-latches) constructed with NOR and NAND gates, along with Data latches (D-latches), are designed with simplicity, autonomy, and reusability. Furthermore, addition, subtraction, and reversible counters leveraging these foundational circuits are successfully constructed. These latches are further applied to transient miRNA recording, environmental toxin detection, and real-time ATP imaging in living cells.
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PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Tao Liao,Dan Hou,Hao Huang,María Belén Camarada,Xiaoning Liao
Herein, a novel ratiometric molecularly imprinted electrochemical sensor (MIP-RECS) based on Ag nanoparticles (AgNPs) and ionic liquid (IL) co-modified Ti 2 NTx (AgNPs@Ti 2 NT x @IL) was developed for the determination of patulin (PAT). Firstly, the AgNPs@Ti 2 NT x @IL nanocomposite, was prepared by an in situ reduction and self-assembled method, and then, was cast on the glassy carbon electrode (GCE) to build an electrochemical interface with excellent conductivity and high surface area. After that, the MIP was deposited on the nanocomposite modified GCE by the electro-polymerization technique, with thionine (Thi) and PAT as functional monomer and template molecules. In the sensing platform, the oxidation peaks generated by Thi and AgNPs were used as reference and probe signal, respectively. The introduction of reference effectively eliminates the influence of the matrix effect. At the same time, the oxidation signal of the Ag probe is greatly improved by the high conductivity and large surface area of the AgNPs@Ti 2 NT x @IL nanocomposite. Therefore, the MIP-RECS presented a wide linear range, a very low low limit of detection (LOD), together with high selectivity and excellent repeatability. Additionally, the linearity ranges and LODs of the sensor can be controlled by the amount of Ag, highlighting its robustness in different types of real samples. Furthermore, this sensing model can be used to sensitively detect other residues with poor redox activity, such as imidacloprid and catechol, highlighting its versatility and universality.
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高影响因子
TALANTA
·
2026
·
IF 6.7
Kaiwen Wang,Peifang Chen,Gengli Huang,Xiaodong Lu,Yating Song,Zhouping Wang
Fumonisin B 1 (FB 1 ) is a secondary metabolite produced by Fusarium species and is considered one of the most dangerous mycotoxins. It is widely present in maize and other agricultural commodities, posing severe threats to human health and animal husbandry. Consequently, the development of a convenient and rapid detection method is of paramount importance. In this work, a metal-organic framework (MOF)-based aptamer biosensor was constructed for the detection of FB 1 , in which aptamer-regulated nanozyme activity was employed to modulate both colorimetric and fluorescent signals. The synthesized Zr-Ce-MOF exhibits intrinsic peroxidase-like activity and fluorescence properties, enabling the catalytic oxidation of colorless o-phenylenediamine (OPD) into yellow oxidized OPD (oxOPD), thereby generating a measurable colorimetric signal. Simultaneously, the oxOPD quenches the inherent fluorescence of the MOF via a fluorescence resonance energy transfer (FRET) effect. Upon target recognition, the aptamer competitively dissociates from the MOF surface, restoring the catalytic activity of the nanozyme and resulting in an increased colorimetric signal and a decreased fluorescence signal, thereby enabling effective dual-mode detection.Under optimized conditions, the limits of detection (LODs) for FB1 were 5.97 ng/mL in colorimetric mode and 1.2 ng/mL in fluorescent mode. The recovery rates for the colorimetric and fluorescent assays ranged from 101.43% to 105.80% and 99.62% to 110.60%, respectively. This proposed dual-mode aptasensor demonstrates excellent analytical performance and provides a robust strategy for the future development of sensing platforms for agricultural contaminants and antibiotic residues.
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高影响因子
ANALYTICA CHIMICA ACTA
·
2026
·
IF 6.1
Xiaoyuan Ma,Xi Ma,Muxi Zhang,Jinchi Han,Zhouping Wang
Background As one of the most common mycotoxins, Zearalenone (ZEN) not only contaminates cereal crops but also exists in processed grain products for its chemical stability. After entering human body through the food chain, ZEN exhibits remarkable bioaccumulation properties, which poses varying degrees of harmful effects to human health. The traditional chromatography instrumental methods require expensive equipment and complex experimental procedures. The aim of this manuscript is to develop a facile and accurate method for ZEN detection in food samples. Results Here, a SERS platform was developed for ZEN detection using COF and noble metal nanomaterial with nitroreductase (NTR)-like activity. First, magnetic COF loaded with gold nanoparticles (MCOF-Au) were synthesized as SERS enhancement substrates with partial NTR activity. Simultaneously, palladium-loaded dumbbell-shaped gold nanorods (AuNR/Pd) were prepared and served as the primary NTR in the 4-NTP/NaBH 4 system, significantly enhancing both catalytic activity and SERS signal. After nucleic acid functionalization, the two components assembled. The addition of ZEN regulated the assemble of AuNR/Pd on MCOF-Au. Following magnetic separation, the catalytic and SERS activities changed in the precipitate. Using the Raman peak of the output product 4-ATP at 390 cm −1 as the quantitative marker, the method showed a linear range in 0.001 μg/kg-1000 μg/kg. The LOD was calculated as 1.88 × 10 −4 μg/kg, and spiked recoveries in wheat flour and corn ranged from 88.91% to 115.78%, demonstrating high sensitivity and reliability for real-sample detection. Significance This aptasensor integrates the high sensitivity of Raman signals with the selectivity of aptamers, providing a robust and efficient tool for accurate ZEN detection in food. COF based nanocomposites are proved to have remarkable NTR activity and the catalytic product 4-ATP exhibits prominent Raman signal, making it suitable for quantitative detection. This design offers a versatile platform for food safety monitoring and public health protection.
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高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 190
·期 1
·1-10
·
IF 6.408
Zhu Jing,Xu Wenxing,Yang Ye,Kong Rongmei,Wang Junmei
A nanozyme sensor array based on the ssDNA-distensible C3N4 nanosheet sensor elements for discriminating multiple mycotoxins commonly existing in contaminated cereals has been explored. The sensor array exploited (a) three DNA nonspecific sequences (A40, T40, C40) absorbed on the C3N4 nanosheets as sensor elements catalyzing the oxidation of TMB; (b) the presence of five mycotoxins affected the catalytic activity of three nanozymes with various degrees. The parameter (A 0 -A) was employed as the signal output to obtain the response patterns for different mycotoxins with the same concentration where A 0 and A were the absorption peak values at 650 nm of oxTMB in the absence and presence of target mycotoxins, respectively. After the raw data was subjected to principal component analysis, 3D canonical score plots were obtained. The sensor array was capable of separating five mycotoxins from each other with 100% accuracy even if the concentration of the mycotoxins was as low as 1 nM. Moreover, the array performed well in discriminating the mycotoxin mixtures with different ratios. Importantly, the practicality of this sensor array was demonstrated by discriminating the five mycotoxins spiking in corn-free samples in 3D canonical score plots, validating that the sensor array can act as a flexible detection tool for food safety. Graphical abstract A nanozyme sensor array was developed based on the ssDNA-distensible C3N4 NSs sensor elements for discriminating muitiple mycotoxins.
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高影响因子
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