ACS Applied Nano Materials
·
2022
·
IF 6.14
Liangliang Zhou,Bin Yu,Lilin Huang,Huiqun Cao,Danying Lin,Yingying Jing,Faiz Wali,Junle Qu
Colloidal quantum dots (QDs) are vital fluorescent probes with several optical features superior to those found in organic dyes for microscopy imaging. However, the emission blinking behavior of a single QD is an intrinsic drawback for stimulated emission depletion microscopy (STED) or structured illumination microscopy techniques. Herein, we present a rational strategy for the synthesis of a type of deterministic, environmentally friendly, small nonblinking core–multishell InP/ZnSe/ZnS QD using a hydrofluoric acid (HF) etching passivation. The strategy includes four steps: synthesis of the InP core, synthesis of oil-phase core–multishell InP/ZnSe/ZnS QDs using hydrogen fluoride and trioctylphosphine etching passivation, promotion of the water solubility of QDs modified by 3-mercaptopropionic acid (MPA), and enhanced biotargeting of core–multishell InP/ZnSe/ZnS QDs by surface coupling technology. The suppressed blinking mechanism was primarily attributed to surface wrapping shells and HF etching passivation of surface dangling bonds, which reduced surface nonradiative recombination. Nonblinking QDs exhibit high fluorescence efficiency (∼90%), good photostability (>20 min), a large Stokes shift (>135 nm), biocompatibility of the cell viability (>90%), and high targeting. Furthermore, super-resolution multifocal structured illumination microscopy imaging of subcellular structures labeled using these QDs confirmed a resolution improvement of greater than 2.6-fold compared to wide-field microscopy. The results indicate that the nonblinking QDs can function as excellent probes for use in the long-term super-resolution visualization of subcellular dynamics.
研究领域:
生物成像
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材料科学
·
光学
✓ 已验证
DOI
高影响因子
Journal of Materials Chemistry C
·
2015
·Vol. 3
·期 41
·10907-10915
·
IF 4.696
Haie Zhu,Bing Yan,Shuxue Zhou,Zengbo Wang,Limin Wu
Microspheres can function as optical superlenses for nanoscale super-resolution imaging. The imaging performance is mainly affected by the size and refractive index of the microsphere. Precise control of these parameters is a challenging task but of fundamental importance to the further development of the technique. In this study, we demonstrate for the first time a nanoparticle-hybrid suspension polymerization approach to chemically synthesize high-quality microspheres (ZrO2/polystyrene) with optical properties that are highly controllable. Microspheres of different sizes (d: 2–20 μm) and refractive indexes (np: 1.590–1.685) were synthesized and their super-resolution imaging performances were evaluated and compared. Our results show that continuously increasing the refractive index of microspheres can enhance the imaging resolution and quality. A 60 nm resolution has been obtained in the wide-field imaging mode and a 50 nm resolution has been obtained in the confocal mode imaging of semiconductor chip samples. The obtained 50–60 nm resolutions have significantly gone beyond the conventional 200 nm resolution limit for visible light optical microscopes; the super-resolution mechanism has been discussed. The synthesized microsphere superlenses may find applications in many other areas as well, including nanolithography, nano-sensing, nano-diagnosis, nano-spectroscopy and ultra-high density optical data storage.
研究领域:
光学
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材料科学
·
纳米技术
✓ 已验证
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Xuebing Wen,Weiluo Ouyang,Zekai Chen,Yuyang Pu,Yifan Feng,Xiao-Fang Jiang,Lakshminarayana Polavarapu,Guofu Zhou,Xiaowen Hu
Laser direct writing enables precise tailoring and patterning of semiconductor materials at the micro-and nanoscale, which is crucial for optoelectronic devices. However, the resolution of laser writing is limited by the diameter of the Airy disk. Herein, a femtosecond (fs) laser super-resolution writing (FsLSRW) technique is demonstrated for subwavelength patterning of stable perovskite nanostructures, achieving feature sizes as small as λ/10, with the line width reaching 80 nm. By leveraging the fs-laser's flexible, precise, and non-thermal diffused patterning capabilities, multicolor perovskite patterns are successfully produced with arbitrary design and pixel arrays. The multicolor perovskite patterns exhibit high hydrolytic, oxidative, and thermal stability due to their encapsulation in a polymer matrix. Furthermore, through precise adjustment of the laser focus plane, the writing of different information is demonstrated on two distinct spatial planes within a double-layer stacked perovskite composite film, enabling the production of dynamic 3D codes at the micro- and nanoscale. The high-efficiency and precision of FsLSRW technology pave a novel path for perovskite devices in fields such as information security, data storage, and optical encryption.
研究领域:
光学
·
材料科学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
MACROMOLECULES
·
2014
·
IF 5.927
Chong Li,Zhe Hu,Matthew P. Aldred,Ling-Xi Zhao,Hui Yan,Guo-Feng Zhang,Zhen-Li Huang,Alexander D. Q. Li,Ming-Qiang Zhu
In this report, we integrated a photoswitchable quencher, a highly emissive yet pH sensitive fluorophore, and a water-soluble polymer with controlled hydrophilicity into a functional nanosystem. The photoswitching quencher is based on dithienylethene (DTE) unit, while the pH-sensitive emitter is naphthalimide (NI) unit and the water-soluble polymer is obtained from polymerization of N-isopropylacrylamide (NIPAM). Together, these three units create a novel super-resolution lysosome highlighter, although individually none can function as a lysotracker. Because the emitter is sensitive to pH, the resulting polymer becomes highly fluorescent in acidic lysosomes. In addition, photoswitching regulates the fluorescence on and off and thus enables super-resolution localization of fluorescent polymers with sub-40 nm spatial resolution in imaging subcellular organelles. Thus, the concept presented here, including the photoswitchable DTE-pH-sensitive NI dyad, is promising to guide the development of future-generation super-resolution imaging agents.
研究领域:
细胞生物学
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生物成像
·
材料科学
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Ning Xu,Qinglong Qiao,Yi Tao,Pengjun Bao,Yinchan Zhang,Wei Zhou,Jin Li,Zhifeng Li,Zhaochao Xu
Subcellular pH plays a key role to modulate protein structures or specific active sites to impact diverse biological processes. The heterogeneity and high dynamics of intracellular pH require probes that can detect pH in the vicinity of the protein and have a wide pH response range. Here, we reported a protein proximity ligation fluorescent probe for super-resolution imaging of subcellular pH dynamics. Relying on SNAP-tag protein labeling technology, the pH-sensitive fluorophore BG-NDM based on photo-induced electron transfer mechanism was localized to the adjacent positions of the target protein in different subcellular organelles. The equilibrium between the non-fluorescent aggregate and the fluorogenic monomer enabled BG-NDM to have the ability of wash-free super-resolution imaging in live cells. And the wide pH response ranges from pH 6 to pH 12 made the probe have the ability of recognizing pH dynamics. The pH changes around functional proteins during mitophagy, cellular alkalosis and acidosis were revealed. Through long-term super-resolution imaging, we could further record the pH dynamics around mitochondrial proteins COX8A during mitochondrial contacts, fusion and fission at the resolution of a single mitochondria.
研究领域:
生物化学
·
细胞生物学
·
分子影像学
✓ 已验证
DOI
高影响因子
OPTICS EXPRESS
·
2025
·Vol. 33
·期 9
·20023-20036
·
IF 3.3
Yuhan Nie,Yuhan Nie,Jie Hu,Siying Zhang,Xiangzhou Meng,Xiangzhou Meng,Guo Fu,Guo Fu,Guo Fu
Single-molecule localization microscopy (SMLM) breaks the diffraction limit, significantly enhancing optical resolution and providing a powerful tool for imaging complex biological structures in life sciences. However, imaging biological structures below 10 nm and studying protein-protein interactions remains a challenge for optical super-resolution microscopy. Here, we introduce a microscopy based on two-color single-molecule localization microscopy, we call it molecular Imaging by two-color imaging (MITI), which improves resolution to the molecular level, reaching several nanometers. We demonstrate MITI is suitable for samples labeled with organic dyes and photoactivatable/photoswichable fluorescent proteins. MITI can resolve two dyes separated by ssDNA with 2-10 nm length as well as ffDronpa and PAmCherry1 fused to the N- and C-terminal of FtsZ (ffDronpa-FtsZ-PAmCherry1). MITI is versatile and easy to realize, and thus, it will expand the applications of molecular scale super-resolution microscopy in life sciences.
研究领域:
生物物理学
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细胞生物学
·
显微技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2024
·
IF 5.3
Peng Xu,Simei Zhong,Yan Ma,Xinxin Duan,Yunfei Wei,Meng Zhang,Jing Xu,Yu-Hui Zhang
Vesicle entrapment remains a major bottleneck for the application of fluorescent nanoprobes (FNPs) with excellent optical properties in the field of super-resolution imaging of subcellular structures in living cells. Here, we developed a cell-penetrating peptide, fR8, which enables efficient cytoplasmic delivery of 3.5 nm FNPs instead of being trapped in vesicles, with a delivery efficiency of up to 75.6%. Further, we have successfully combined fusion protein technology with FNPs-fR8 to achieve high specificity and stable labeling of FNPs to various subcellular structures within live cells. Utilizing the advantages of FNPs, such as high brightness and resistance to photobleaching, FNPs modified with fR8 and the HaloTag ligand can be used for long-term super-resolution imaging of subcellular structures in living cells, allowing us to study the dynamic interactions between them. This work provides a practical tool for applying FNPs to live-cell super-resolution imaging and expands the application of super-resolution imaging technology in live-cell studies.
研究领域:
生物化学
·
分子生物学
·
细胞生物学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Chemistry
·
2021
·
IF 5.221
Han Yubing,Zhang Zhimin,Liu Wenjie,Yao Yuanfa,Xu Yingke,Liu Xu,Kuang Cuifang,Hao Xiang
Despite the urgent need to image living specimens for cutting-edge biological research, most existing fluorescent labeling methods suffer from either poor optical properties or complicated operations required to realize cell-permeability and specificity. In this study, we introduce a method to overcome these limits—taking advantage of the intrinsic affinity of bright and photostable fluorophores, no matter if they are supposed to be live-cell incompatible or not. Incubated with living cells and tissues in particular conditions (concentration and temperature), some Atto and BODIPY dyes show live-cell labeling capability for specific organelles without physical cell-penetration or chemical modifications. Notably, by using Atto 647N as a live-cell mitochondrial marker, we obtain 2.5-time enhancement of brightness and photostability compared with the most commonly used SiR dye in long-term imaging. Our strategy has expanded the scientist's toolbox for understanding the dynamics and interactions of subcellular structures in living specimens.
研究领域:
分子生物学
·
细胞生物学
·
生物物理学
·
成像科学
✓ 已验证
DOI
PubMed
高影响因子
MACROMOLECULES
·
2017
·
IF 5.835
Haie Zhu,Min Chen,Shuxue Zhou,Limin Wu
A conventional optical microscope fails to resolve features with sizes smaller than 200 nm due to the Abbe diffraction limit. Herein, we synthesize two kinds of high refractive index and shape controllable colloidal polymer microspheres, poly(9,9′-bis[4-(2-acryloyloxyethyloxy)phenyl]fluorene) (poly(BAEPF)) and poly(2-phenylphenoxyethyl acrylate) (poly(OPPEA)), used for solid immersion lens. It is the high refractive index and close contact with the surface features that can make the colloidal polymer microsphere-based microlens effectively collect evanescent information on the substrate and form a clear and magnified virtual image at certain position below the substrate surface. Thus, as small as 60 nm feature size on the substrate can be clearly resolved through the microlens under a visible light optical microscope, which is far beyond the resolution limit of a visible light microscope.
研究领域:
材料科学
·
光学
·
显微技术
✓ 已验证
DOI
高影响因子
Laser & Photonics Reviews
·
2025
·
IF 10
Zhuang-zhuang Zhou,Meng-meng Li,Ning Wei,Zheng Sun,Xi-lin Li,Hao Liu,Jian-gen Wu,Hong Xia
Conductive polymer poly(3, 4-ethylenedioxythiophene): polystyrene sulfonate (PEDOT:PSS) has important applications in multiple fields of optoelectronics. Combining micronano fabrication methods, high-resolution PEDOT:PSS patterns can be obtained, but doping with photosensitive resins as a main method often compromises intrinsic properties, especially conductivity. Therefore, creating high-conductivity and super-resolution PEDOT:PSS patterns without relying on doped photoresists remains crucial. In this study, a femtosecond laser-induced dissociation and reconstruction (FLIDR) is utilized to fabricate PEDOT:PSS patterns with conductivity up to 851 S cm −1 and the highest resolution reported to date less than 400 nm. Additionally, 3D spatial micropatterns of PEDOT:PSS are achieved without the use of photopolymer resins for the first time. The electromagnetic interference (EMI) shielding performance of PEDOT:PSS obtained through FLIDR is evaluated, indicating an efficient EMI shielding effectiveness of 65.5 dB at a thickness of just 0.045 mm and an exceptional specific shielding effectiveness of 1455.6 dB mm −1 . Meanwhile, the high-resolution PEDOT:PSS patterns are used to develop an all-solid-state electrochromic (EC) device with an ultrafast response of less than 0.4 s and excellent stability. Further, the EC device is also utilized in multi-level covert anti-counterfeiting.
研究领域:
材料科学
·
光学工程
·
电子工程
✓ 已验证
产品关联
DOI
高影响因子
ACS Nano
·
2021
·
IF 15.881
Xindong Wang,Xinyu Wang,Bo Qu,Nuernisha Alifu,Ji Qi,Ruiyuan Liu,Qinrui Fu,Ruifang Shen,Qi Xia,Lijun Wu,Bing Sun,Jibin Song,Youping Lin,Xin Huang,Anjun Qin,Jun Qian,Ben Zhong Tang,Guanying Chen
Molecular organic dyes are classic fluorescent nanoprobes finding tremendous uses in biological and life sciences. Yet, they suffer from low brightness, poor photostability, and lack of functional groups for bioconjugation. Here, we describe a class of biocompatible dye–protein optical nanoprobes, which show long-time photostability, superbrightness, and enriched functional groups. These nanoprobes utilize apoferritin (an intracellular protein for iron stores and release) to encase appropriate molecular organic dyes to produce on-demand fluorescence in aqueous solution. A pH-driven dissociation–reconstitution process of apoferritin subunits allows substantial incorporation of hydrophilic (aggregation caused quenching, ACQ) or hydrophobic (aggregation induced enhancement, AIE) dye molecules into the protein nanocavity (8 nm), producing monodispersed dye–apoferritin nanoparticles (apo-dye-NPs, ∼12 nm). As compared with single dye monomer, single apo-dye-NPs possess hundreds of times larger molar extinction coefficient and 2 orders of magnitude higher absolute luminescence quantum yield (up to 45-fold), multiplying fluorescence brightness up to 2778-fold. We show that varying the type of incorporated dyes entails a precise control over nanoprobe emission profile tunable in a broad spectral range of 370–1300 nm. Mechanical investigations indicate that the diversified microstructures of nanocavity inner surface are able to conform ACQ dyes at reasonable space interval while providing protein-guided-stacking for AIE dyes, thus enhancing fluorescence quantum yield through confining intermolecular quenching and intramolecular rotation. Moreover, apo-dye-NPs are able to emit stable fluorescence (over 13 min) without quenching in confocal imaging of HepG2 cancer cell under ultrahigh laser irradiance (1.3 × 106 W/cm2). These superb properties make them suitable, as demonstrated in this work, for long-term super-resolved structured illumination microscopic cell imaging (spatial resolution, 117 nm) over 48 h, near-infrared (NIR) fluorescence angiography imaging of whole-body blood vessels (spatial resolution, 380 μm), and NIR photoacoustic imaging of liver in vivo.
研究领域:
生物化学
·
分子生物学
·
医学影像学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nature Photonics
·
2024
·
IF 32.3
Shan Xuchen,Ding Lei,Wang Dajing,Wen Shihui,Shi Jinlong,Chen Chaohao,Wang Yang,Zhu Hongyan,Huang Zhaocun,Wang Shen S. J.,Zhong Xiaolan,Liu Baolei,Reece Peter John,Ren Wei,Hao Weichang,Lu Xunyu,Lu Jie,Su Qian Peter,Chang Lingqian,Sun Lingdong,Jin Dayong,Jiang Lei,Wang Fan
Precise force measurement is critical to probe biological events and physics processes, spanning from molecular motor’s motion to the Casimir effect, as well as the detection of gravitational waves. Yet, despite extensive technological developments, the three-dimensional nanoscale measurement of weak forces in aqueous solutions still faces major challenges. Techniques that rely on optically trapped nanoprobes are of significant potential but are beset with limitations, including probe heating induced by high trapping power, undetectable scattering signals and localization errors. Here we report the measurement of the long-distance interaction force in aqueous solutions with a minimum detected force value of 108.2 ± 510.0 attonewton. To achieve this, we develop a super-resolved photonic force microscope based on optically trapped lanthanide-doped nanoparticles coupled with nanoscale three-dimensional tracking-based force sensing. The tracking method leverages neural-network-empowered super-resolution localization, where the position of the force probe is extracted from the optical-astigmatism-modified point spread function. We achieve a force sensitivity down to 1.8 fN Hz –1/2 , which approaches the nanoscale thermal limit. We experimentally measure electrophoresis forces acting on single nanoparticles as well as the surface-induced interaction force on a single nanoparticle. This work opens the avenue of nanoscale thermally limited force sensing and offers new opportunities for detecting sub-femtonewton forces over long distances and biomechanical forces at the single-molecule level.
研究领域:
生物物理学
·
纳米技术
·
光学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）