COLLOIDS AND SURFACES B-BIOINTERFACES
·
2018
·
IF 3.997
Jinbiao Gao,Baiqing Yu,Chao Li,Ming Xu,Zhong Cao,Xiaoyan Xie,Wei Wang,Jie Liu
Ultrasound-triggered delivery system is among the various multifunctional and stimuli-responsive strategies that hold great potential as a robust solution to the challenges of localized drug delivery and gene therapy. In this work, we developed an ultrasound-triggered delivery system PFP/C 9 F 17 -PAsp(DET)/CAD/PGA- g -mPEG nanodroplet, which combined ultrasound responsive phase-change contrast agent, acid-cleavable doxorubicin prodrug and cationic amphiphilic fluorinated polymer carrier, aiming to achieve both high imaging contrast and preferable ultrasound-triggered anti-cancer therapeutic effect. The optimized nanodroplets were characterized as monodispersed particles with a diameter of about 400 nm, slightly positive surface charge and high drug-loading efficiency. The functional augmenter PGA- g -mPEG provided the nanodroplets good sustainability, low cytotoxicity and good serum compatibility, as confirmed by stability and biocompatibility tests. In ultrasound imaging study, the nanodroplets produced significant contrast with ultrasound irradiation (3.5 MHz, MI = 0.08) at 37 ℃. Cell uptake and cytotoxicity studies in HepG2 and CT-26 cells showed the enhanced drug uptake and therapeutic effect with the combination of nanodroplets and ultrasound irradiation. These results suggest that the PFP/CAD-loaded phase change nano-emulsion can be utilized as an efficient theranostic agent for both ultrasound contrast imaging and drug delivery.
研究领域:
医学影像学
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药剂学
·
肿瘤学
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2023
·
IF 4.2
Shuainan Zhao,Chaoqun Yao,Lixue Liu,Mei Yang,Guangwen Chen
Ultrasound emulsification, including the emulsification mechanism as well as the stability and size distribution of the prepared emulsion, was systematically investigated in laboratory-built ultrasonic microreactors. The respective cavitation-induced emulsification processes in hydrophilic and hydrophobic microchannels were first analyzed, after which a bubble shuttling and dispersing mechanism was proposed. The type of prepared emulsion was found to be strongly associated with the hydrophilic–lipophilic balance (HLB) value and water/oil ratio of the emulsified system, but was hardly affected by the ultrasound parameters. The size distribution of the prepared emulsion was characterized using both microscopy and dynamic scattering technique, and was measured to be in the range of 700–1800 nm. It was also found that higher ultrasound intensity, lower frequency, and larger residence time are more favorable to producing fine emulsion. Finally, an operation strategy was proposed on the basis of the experimental results, expecting to provide recommendations for the application of ultrasonic microreactors for continuous emulsification.
研究领域:
化学工程
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材料科学
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生物技术
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产品关联
DOI
高影响因子
AICHE JOURNAL
·
2022
·Vol. 68
·期 8
·e17689
·
IF 4.167
Qiang Zhang,Zhengya Dong,Zhikai Liu,Guangwen Chen
This study aims to investigate the effect of ultrasonic waveforms on the gas–liquid mass transfer process. For a given load power ( P ), continuous rectangular wave yielded stronger bubble oscillation and higher mass transfer coefficient ( k L a ) than continuous triangular and sinusoidal wave. For pulsed ultrasound, the k L a decreased monotonically with decreasing duty ratio ( D ), resulting in weak enhancement at low D (≤33%). For a given average load power ( P A ), concentrating the P for a shorter period resulted in a higher k L a due to stronger cavitation behavior. For a given P A and D , decreasing the pulse period ( T ) led to an increase in k L a , which reached a constant high level when the T fell below a critical value. By optimizing the D and T , a k L a equivalent to 92% of that under continuous ultrasound was obtained under pulsed ultrasound at a D of 67%, saving 33% in power consumption.
研究领域:
化学工程
·
声化学
·
传质学
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产品关联
DOI
高影响因子
RSC Advances
·
2023
·Vol. 13
·期 28
·19065-19078
·
IF 3.9
Xia Ou,Zhong Zhang,Li Lin,Yan Du,Yu Tang,Yaotai Wang,Jianzhong Zou
Objective: To make up for the insufficient ultrasound ablation of tumors, the energy output or synergist is increased but faces the big challenge of normal tissue damage. In this study, we report a tumor-homing bacterium, Bifidobacterium bifidum (B. bifidum), adsorbing liposomes that encapsulate perfluorohexane (PFH) and doxorubicin (DOX) to enhance the pulsed-focused ultrasound (PFUS) for tumor therapy, so as to improve the efficacy, safety and controllability of ultrasound treatment. Methods: The PFH and DOX co-loaded cationic liposomal nanoparticles (CL-PFH-DOX-NPs) were prepared for ultrasound (US) imaging, cell-killing, and B. bifidum adsorption for the reactive oxygen species (ROS) testing. The aggregation of B. bifidum and CL-PFH-DOX-NPs is called tumor-homing aggregation (B. bifidum@CL-PFH-DOX-NPs) in this study, and the synergistic effects of B. bifidum@CL-PFH-DOX-NPs were analyzed in vivo. Results: Comprehensive studies validated that CL-PFH-DOX-NPs can enhance US imaging and cell-killing and B. bifidum can promote ROS, and B. bifidum@CL-PFH-DOX-NPs achieve PFUS synergism in vivo. Importantly, active homing of B. bifidum facilitated the delivery and retention of CL-PFH-DOX-NPs in tumors, reducing dispersion in normal tissues, achieving the targeting ability of B. bifidum@CL-PFH-DOX-NPs. The best sonication time was chosen according to the distribution of CL-PFH-DOX-NPs in vivo to achieve efficient therapy. Especially, B. bifidum@CL-PFH-DOX-NPs amplified cavitation and the immune-boosting effects. Conclusion: Multifunctional B. bifidum@CL-PFH-DOX-NPs were successfully constructed with well targeting, which not only realized US imaging monitoring, strong cavitation and complementary killing during PFUS, but also achieved immunity enhancement after PFUS. The combination of PFUS, B. bifidum and CL-PFH-DOX-NPs provides a new idea for the potential application of ultrasound therapy in solid tumors.
研究领域:
肿瘤学
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生物医学工程
·
药学
·
治疗超声
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产品关联
DOI
PubMed
高影响因子
Cell Reports Medicine
·
2024
·
IF 11.7
Yaozhang Yang,Yuanyuan Wang,Fengyi Zeng,Yuhao Chen,Zhiyi Chen,Fei Yan
Our previous work developed acoustic response bacteria, which enable the precise tuning of transgene expression through ultrasound. However, it is still difficult to visualize these bacteria in order to guide the sound wave to precisely irradiate them. Here, we develop ultrasound-visible engineered bacteria and chemically modify them with doxorubicin (DOX) on their surfaces. These engineered bacteria (Ec@DIG-GVs) can produce gas vesicles (GVs), providing a real-time imaging guide for remote hyperthermia high-intensity focused ultrasound (hHIFU) to induce the expression of the interferon (IFN)-γ gene. The production of IFN-γ can kill tumor cells, induce macrophage polarization from the M2 to the M1 phenotype, and promote the maturation of dendritic cells. DOX can be released in the acidic tumor microenvironment, resulting in immunogenic cell death of tumor cells. The concurrent effects of IFN-γ and DOX activate a tumor-specific T cell response, producing the synergistic anti-tumor efficacy. Our study provides a promising strategy for bacteria-mediated tumor chemo-immunotherapy.
研究领域:
医学
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生物技术
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肿瘤学
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产品关联
DOI
PubMed
高影响因子
CURRENT PHARMACEUTICAL DESIGN
·
2018
·Vol. 24
·期 30
·3587-3595
·
IF 2.757
Yang Feng,Li Yue,Liufu Chun,Wang Yi,Chen ZhiYi
Purpose: Ultrasound contrast agents involving a therapeutic drug applied during enhanced imaging can be used for targeted therapy. The preparation of contrast agents is a precondition and basis for the use of multifunctional contrast agents in molecular imaging. Methods: This study
uses thin-film hydration-mechanical vibration to carry out the preliminary preparation of a cationic contrast agent (CCA); characterizes the particle diameter, potential, distribution, and concentration of the agents; and optimizes the factors affecting the preparation of the agents.
Results: This study found that thin-film hydration-mechanical vibration methods offer a better preparation effectiveness and achieve smaller particle diameters and more even distributions, as well as give better imaging performance. Different concentrations of CCA and plasmid and different
gene transfection methods can produce different degrees of sonoporation to achieve optimal transfection efficiency. Ultrasound parameters have a great influence on transfection efficiency and plasmid integrity. A previous study confirmed that the ultrasound parameters of 1 MHz, 1 W/cm2, a
duty cycle controller (DC) of 20%, and irradiation for 1 min can well deliver genes to tumor cells, with little impact on cell survival. Conclusion: Our findings indicate that ultrasound-targeted microbubble destruction (UTMD)-mediated CCA destruction facilitates gene transfection and
may represent an effective gene delivery method for cervical cancer therapy.
研究领域:
医学影像学
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基因治疗
·
肿瘤学
✓ 已验证
DOI
PubMed
高影响因子
Nanoscale
·
2022
·Vol. 14
·期 9
·3545-3553
·
IF 8.307
Di Sun,Jie Liu,Lijuan Xue,Li Li,Daoyin Xie,Shilin Li,Shengmei Li,Xinlian Wang,Dongtao Yin,Zhaoqi Ren,Ru Bai,Wei Guo,Ying Liu,Chunying Chen
Superficial thrombophlebitis is one of the most significant complications of superficial vein thrombosis. Rapid imaging and mapping with high resolution is particularly important for accurate diagnosis so as to carry out treatment as soon as possible. Ultrasound imaging technology has been used extensively because of the low-cost, minimal invasiveness, and convenient application in clinical practice. And the ultrasonic couplant is an essential component in ultrasound examination. However, when imaging superficial structures, traditional liquid ultrasonic couplants often produce inadequate results. In this study, we investigate whether a hydrogel membrane can be used to improve the imaging of superficial vessels. To this end, we generated a polyacrylamide-bacterial nanocellulose hydrogel membrane (PAM-BC) that efficiently forms at 60 °C in only 10 min by redox polymerization. With PAM-BC-2.5, it was possible to acquire high resolution intravascular ultrasound images to assess superficial vessels in humans and the superficial vasculature in rats and miniature pigs using various brands of ultrasound instruments. The PAM-BCs represent a new, solid ultrasonic membrane which is suitable for diagnosing disease in superficial vessels.
研究领域:
医学影像
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生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Materials
·
2020
·
IF 2.705
Xiao Ruolei,Zhao Zhiwei,Chen Jiajuan,He Liu,Wang Huili,Huang Lingping,Luo Binhua
In the study reported here, polylactic acid (PLLA) polymer was synthesized using stannous octoate (Sn(Oct)2) and N-(t-butoxycarbonyl) ethanolamine (EABoc) as the catalyst and the initiator, respectively. The selected PLLA polymer with proper molecular weight was used to prepare nanobubbles encapsulating with liquid perfluoropentane. Then, lactoferrin (Lf), which has a good affinity with tumor cells, was conjugated to PLLA nanobubbles. The resulting Lf–PLLA nanobubbles were examined from the perspective of appearance, size, zeta potential, and stability in vitro. The average hydrodynamic diameter of the Lf–PLLA nanobubbles was 315.3 ± 4.2 nm, the polydispersity index (PDI) was 0.153 ± 0.020, and the zeta potential was around −11.3 ± 0.2 mV. Under the transmission electron microscope (TEM), Lf–PLLA nanobubbles were highly dispersed and had a spherical shape with a distinct capsule structure. The Lf–PLLA nanobubbles also showed little cytotoxicity and low hemolysis rate and exhibited good stability in vitro. The enhanced ultrasound imaging ability of Lf–PLLA nanobubbles was detected by an ultrasound imaging system. The results of ultrasound studies in vitro showed that the liquid perfluoropentane underwent phase transition under ultrasonic treatment, which proved the Lf–PLLA nanobubbles could enhance the ability of ultrasonic imaging. The studies of ultrasonic imaging in nude mice bearing subcutaneous tumors showed that the ability of enhanced ultrasonic images was apparent after injection of Lf–PLLA nanobubbles. Acoustic behavior in vitro and in vivo showed that the Lf–PLLA nanobubbles were characterized by strong, stabilized, and the ability of tumor-enhanced ultrasound imaging. Thus, the Lf–PLLA nanobubbles are an effective ultrasound contrast agent for contrast-enhanced imaging.
研究领域:
材料科学
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生物医学工程
·
超声成像
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聚合物化学
·
癌症研究
✓ 已验证
DOI
高影响因子
TUMOR BIOLOGY
·
2016
·Vol. 37
·期 7
·8609-8619
·
IF 2.926
Xu Jinfeng,Zeng Xinxin,Liu Yingying,Luo Hui,Wei Zhanghong,Liu Huiyu,Zhou Yuli,Zheng Hairong,Zhou Jie,Tan Guanghong,Yan Fei
Ultrasound-targeted microbubble destruction (UTMD) has become a novel gene/drug delivery method in cancer therapeutic application. However, the gene transfection efficiency mediated by UTMD is still unsatisfactory. Here, we introduced iRGD/CCR2 dual-targeted cationic microbubbles (MB iRGD/CCR2 ) which was modified with PEI-600 and coated with iRGD peptides and anti-CCR-2 antibodies. It showed that MB iRGD/CCR2 had a 25.83 ± 1.57 mV surface zeta potential and good stability. The experiments in vitro showed MB iRGD/CCR2 had higher binding efficiency with both bEnd.3 cells and MCF-7 cells than that of iRGD or CCR2 single-targeted cationic microbubbles (MB iRGD or MB CCR2 ) ( P < 0.05 for both). Agarose gel electrophoresis assay showed that MB iRGD/CCR2 can effectively load pGPU6/GFP/Neo-shAKT2 plasmid DNA. Compared with the plain MBs (MB control ) or single-targeted cationic MBs including MB iRGD and MB CCR2 ( P < 0.05 for all), the dual-targeted cationic MB iRGD/CCR2 groups had higher gene transfection efficiency under US exposure. It showed that the dual-targeted cationic MB iRGD/CCR2 has a potential value to be used as an ultrasound imaging probe for ultrasound image-guided tumor gene therapy.
研究领域:
医学影像
·
基因治疗
·
癌症研究
·
超声技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2020
·
IF 3.644
Jiang Qiongchao,Zeng Yunting,Xu Yanni,Xiao Xiaoyun,Liu Hejun,Zhou Boyang,Kong Yao,Saw Phei Er,Luo Baoming
Though radiofrequency ablation (RFA) is considered to be an effective treatment for hepatocellular carcinoma (HCC), but more than 30% of patients may suffer insufficient RFA (IRFA), which can promote more aggressive of the residual tumor. One possible method to counter this is to accurately identify the margin of the HCC. Colony-stimulating factor 1 receptor (CSF-1R) has been found to be restrictively expressed by tumor associated macrophages (TAMs) and monocytes which more prefer to locate at the boundary of HCC. Using biotinylation method, we developed a CSF-1R-conjugated nanobubble CSF-1R (NBCSF–1R) using a thin-film hydration method for margin detection of HCC. CSF-1R expression was higher in macrophages than in HCC cell lines. Furthermore, immunofluorescence showed that CSF-1R were largely located in the margin of xenograft tumor and IFRA models. In vitro, NBCSF–1R was stable and provided a clear ultrasound image even after being stored for 6 months. In co-culture, NBCSF–1R adhered to macrophages significantly better than HCC cells (p = 0.05). In in vivo contrast-enhanced ultrasound imaging, the washout half-time of the NBCSF–1R was significantly greater than that of NBCTRL and Sonovue® (p = 0.05). The signal intensity of the tumor periphery was higher than the tumor center or non-tumor region after NBCSF–1R injection. Taken together, NBCSF–1R may potentially be used as a non-invasive diagnostic modality in the margin detection of HCC, thereby improving the efficiency of RFA. This platform may also serve as a complement method to detect residual HCC after RFA; and may also be used for targeted delivery of therapeutic drugs or genes.
研究领域:
医学影像
·
肿瘤学
·
生物医学工程
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Ruihao Yang,Haoran Zhang,Zeinab Marfavi,Quanjie Lv,Yijun Han,Kang Sun,Congli Yuan,Ke Tao
Developing strategies for the treatment of bacterial biofilms is challenging due to their complex and resilient structure, low permeability to therapeutics, and ability to protect resident pathogens. Herein, we demonstrate that a polylysine-stabilized perfluorocarbon nanoemulsion is favored for penetrating biofilms and sensitizing the cavitation effect of low-intensity ultrasound, resulting in the dispersal of extracellular polymeric substances and killing of the protected cells. Through experiments, we observed a complete penetration of the nanoemulsion in a 40 μm Pseudomonas aeruginosa biofilm and demonstrated that it was induced by the fluidic perfluorocarbon, possibly attributing to its low surface tension. Furthermore, we presented an almost complete antibiofilm effect with a low-intensity ultrasound (1 MHz, 0.75 W/cm2, 5 min) in diverse cases, including cultured biofilms, colonized urinary catheters, and chronic wounds. During the treatment process, the perfluorocarbon phase enhanced the number and imploding energy of ultrasound cavities, thoroughly divided the biofilm structure, prevented biofilm self-healing, and sterilized the resident pathogens. Thus, the penetration and sensitization of the nanoemulsion might serve as a facile and potent strategy for eradicating biofilms in various applications.
研究领域:
生物医学工程
·
微生物学
·
治疗技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Today Bio
·
2022
·
IF 10.761
Cuixian Li,Piao Zhu,Huijing Xiang,Yunjie Jin,Beilei Lu,Yujia Shen,Wenping Wang,Beijian Huang,Yu Chen
Thermal ablation (TA), as a minimally invasive therapeutic technique, has been extensively used to the treatment of solid tumors, such as renal cell carcinoma (RCC), which, unfortunately, still fails to overcome the high risk of local recurrence and distant metastasis since the incomplete ablation cannot be ignored due to various factors such as the indistinguishable tumor margins and limited ablation zone. Herein, we report the injectable thermosensitive hydrogel by confining curcumin (Cur)-loaded hollow mesoporous organosilica nanoparticles ( [email protected] @gel) which can locate in tumor site more than half a month and mop up the residual RCC under ultrasound (US) irradiation after transforming from colloidal sol status to elastic gel matrix at physiological temperature. Based on the US-triggered accelerated diffusion of the model chemotherapy drug with multi-pharmacologic functions, the sustained and controlled release of Cur has been demonstrated in vitro . Significantly, US is employed as an external energy to trigger Cur, as a sonosensitizer also, to generate reactive oxygen species (ROS) for sonodynamic tumor therapy (SDT) in parallel. Tracking by the three-dimensional contrast-enhanced ultrasound (3D-CEUS) imaging, the typical decreased blood perfusions have been observed since the residual xenograft tumor after incomplete TA were effectively suppressed during the chemo-sonodynamic therapy process. The high in vivo biocompatibility and biodegradability of the multifunctional nanoplatform confined by thermogel provide the potential of their further clinical translation for the solid tumor eradication under the guidance and monitoring of 3D-CEUS.
研究领域:
肿瘤学
·
生物医学工程
·
影像医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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