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STAR Protocols · 2022 · IF 0

Protocol for detecting substrates in living cells by targeted molecular probes through hyperpolarized 129Xe MRI

Qingbin Zeng,Qianni Guo,Yaping Yuan,Lei Zhang,Weiping Jiang,Yuqi Yang,Xin Zhou

Summary Due to limited detection sensitivity and contrast limitation, imaging substrates with 129 Xe MRI in living cells is still a challenge. Here, we present an effective protocol to detect and image substrates in human lung cancer cells A549 with hyperpolarized 129 Xe MRI. This protocol was optimized for a cryptophane-based probe sensitive to biothiols and can be expanded to other Xe-based probes to detect potential biomarkers in other mammalian cells. For complete details on the use and execution of this protocol, please refer to Zeng et al. (2021) .

研究领域: 医学成像 · 生物医学工程 · 分子生物学 · 癌症研究

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Nature Communications · 2025 ·Vol. 16 ·期 1 ·1-13 · IF 15.7

Multivariate metal-organic frameworks enable chemical shift-encoded MRI with femtomolar sensitivity for biological systems

Zeng Qingbin,Yue Quer,Zhang Ming,Zhou Rui,Wang Zhen,Xiao Zhongyu,Yuan Yaping,Zhao Xiuchao,Jiang Weiping,Zhang Lei,Yang Yuqi,Yang Minghui,Li Haidong,Bouchard Louis-S.,Guo Qianni,Zhou Xin

Fluorescent molecules with specific target moieties are essential for histopathological analysis, but their limited tissue penetration depth makes in vivo, in situ color encoding analysis challenging. Magnetic resonance imaging (MRI) offers deep tissue penetration. When combined with chemical shift-encoded MRI reporters, it enables in vivo chemical shift encoding for biotarget imaging and analysis. These reporters require both strong signal intensity and large chemical shift window. However, conventional proton MRI reporters, with low sensitivity and a small chemical shift window, limit their in vivo applications. Here, we describe a chemical shift-encoded hyperpolarized 129 Xe MRI reporter based on the multivariate metal-organic framework, NiZn-ZIF-8, to overcome these challenges. The proposed NiZn-ZIF-8 gives distinct chemical shifts for dissolved and entrapped 129 Xe without signal interference, enhancing the 129 Xe NMR signal by 210 times compared to dissolved 129 Xe in water and biological media. This enables detection threshold at ≈ 4 fM concentrations, setting a record for the lowest concentration of xenon hosts detected in nanomaterials. Additionally, NiZn-ZIF-8 exhibits good in vivo MRI performance, allowing xenon encoding and distinction in rat lungs. NiZn-ZIF-8 represents a versatile and powerful platform for advanced molecular imaging and in vivo biomedical diagnostics.

CAS / MDL: 693-98-1

研究领域: 医学成像 · 纳米材料 · 分子成像 · 生物医学诊断 · 磁共振成像

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Protocol for detecting substrates in living cells by targeted molecular probes through hyperpolarized 129Xe MRI

Multivariate metal-organic frameworks enable chemical shift-encoded MRI with femtomolar sensitivity for biological systems