Journal of Materials Chemistry C
·
2022
·Vol. 10
·期 41
·15451-15457
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IF 8.067
Xi-Zhu Zhang,Wen-Jing Zhu,Zhong-Xi Yang,Yi Feng,Lin-Lin Fan,Guang-Gang Gao,Hong Liu
The sensitive and selective detection of ethylenediamine (EDA) has great significance due to its threat to environment and human health. Herein, a rationally designed polyoxometalate–viologen complex (1) has been demonstrated, in which the viologen molecules are hydrogen bonded to Dawson-type polyoxoanions. When complex 1 is in the atmosphere of EDA, the chromic intensity or Raman signals present proportional response to EDA concentrations. In particular, a very low detection limit of 0.1 ppb can be obtained for 1 based on its Raman signal output, which is the most sensitive complex than the reported EDA sensors. Also, high selectivity is observed for 1 when other amine molecules coexist as interfering compounds. According to the structural analysis and comparative experimental results, the highly sensitive and selective chromic or Raman response can be ascribed to the existence of viologen in the crystal lattice, which can not only accept electrons from EDA but also effectively accelerate the electron transfer to polyoxometalate. This work affords a novel concept for the design of highly sensitive and dual response polyoxometalate-based sensors by virtue of viologen-boosting electron transfer.
研究领域:
材料科学
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环境科学
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化学传感器
✓ 已验证
DOI
高影响因子
Small
·
2025
·
IF 12.1
Xinxin Liu,Tao Liu,Xueli Yuan,Jing Zhang,Mengjiao Zhu,Wei Zhang,Zhenyu Chu,Wanqin Jin
Viral hepatitis is a highly infectious disease caused by various hepatitis viruses that, thus far, have no cure. In early viral invasion, a low viral load in the blood makes early detection difficult unless the symptoms are severe. Herein, a new ultrasensitive and ultrafast hepatitis virus detection strategy is proposed by constructing a dual-surface-activated nanotube that enables recognition probes to not only bind to the outer surface of the nanotube but also penetrate and lock onto its inner surface. This approach significantly enhances both the specific surface area and number of active sites, thereby improving the capture efficiency for traces of hepatitis viral DNA, such as the B type (HBV-DNA). Compared with current DNA detection methods, this approach eliminates the additional amplification steps to achieve an ultralow detection limit of 10 −18 m , advancing the available detection time as early as possible. Using human serum as a sample, this technique enables the precise and quantitative reporting of HBV-DNA within only 15 min and overcomes the interference of usual proteins and other viral DNA. This technique provides new inspiration for quick and early hepatitis diagnosis for in-hospital and non-hospital patients without bulky instruments and complex operations.
研究领域:
生物技术
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医学诊断
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2015
·
IF 6.723
Lu Wang,Fuqiang Fan,Wei Cao,Huaping Xu
Reactive oxygen species (ROS) play crucial roles in cell signaling and redox homeostasis and are strongly related to metabolic activities. The increase of the ROS concentration in organisms can result in several diseases, such as cardiovascular diseases and cancer. The concentration of ROS in biologically relevant conditions is typically as low as around tens of micromolars to 100 μM H2O2, which makes it necessary to develop ultrasensitive ROS-responsive systems. A general approach is reported here to fabricate an ultrasensitive ROS-responsive system via coassembly between tellurium-containing molecules and phospholipids, combining the ROS-responsiveness of tellurium and the biocompatibility of phospholipids. By using dynamic light scattering, transmission electron microscopy, scanning electron microscopy, and NMR spectra, coassembly behaviors and the responsiveness of the coassemblies have been investigated. These coassemblies can respond to 100 μM H2O2, which is a biologically relevant ROS concentration, and demonstrate reversible redox properties.
研究领域:
生物化学
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材料科学
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DOI
高影响因子
FOOD AND CHEMICAL TOXICOLOGY
·
2022
·
IF 5.572
Ahmet Şenocak,Vildan Sanko,Süreyya Oğuz Tümay,Yasin Orooji,Erhan Demirbas,Yeojoon Yoon,Alireza Khataee
MAX phases have attracted great attention due to unique features such as thermal and electrical conductivity, easy fabrication, heat resistant, and lightweight. In this study, an easy and green method was employed to successfully develop a Ti 3 Al 0.5 Cu 0.5 C 2 MAX phase structure, and a Ti 3 Al 0.5 Cu 0.5 C 2 based glassy carbon electrode (GCE) was applied for the electrochemical determination of rutin antioxidants in mandarin and kiwi samples. The developed Ti 3 Al 0.5 Cu 0.5 C 2 MAX phase was characterized by different techniques such as X-ray photoelectron spectroscopy (XPS), thermogravimetry and differential scanning calorimetry (TG-DSC), X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET), diffuse reflectance spectroscopy (DRS), transmission electron microscopy (TEM), and scanning electron microscopy (SEM) to obtain information on the structural and morphological properties. Electrochemical methods such as cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were employed for the determination of rutin using Ti 3 Al 0.5 Cu 0.5 C 2 /GCE. The GCE modified with Ti 3 Al 0.5 Cu 0.5 C 2 demonstrated amplified electrochemical response (ca. 4.25 times) in comparison to the bare GCE towards rutin, and exhibited ultra-sensitivity and selectivity in the presence of other interfering antioxidants. Under the optimum conditions, good linearity in the range of 0.02–50.00 μmol L −1 was obtained for rutin analysis by the Ti 3 Al 0.5 Cu 0.5 C 2 -based sensor with a limit of detection (LOD, 3σ/K) as low as 0.015 μmol L −1 . The fabricated Ti 3 Al 0.5 Cu 0.5 C 2 MAX phase was applied to determine trace levels of rutin in mandarin and kiwi samples with validation by high-performance liquid chromatography (HPLC), thus highlighting its potential for the electrochemical determination of small molecules in the agricultural field.
研究领域:
食品科学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Jiayue Shi,Sijia Li,Rongguang Shao,Yang Jiang,Yanxia Qiao,Jin Liu,Yaqian Zhou,Yan Li
In this study, an ultrasensitive electrochemiluminescence (ECL) aptasensing method was developed for lipopolysaccharide (LPS) determination based on CRISPR-Cas12a accessory cleavage activity. Tris (2,2'-bipyridine) dichlororuthenium (II) (Ru(bpy) 3 2+ ) was adsorbed on the surface of a glassy carbon electrode (GCE) coated with a mixture of gold nanoparticles (AuNPs) and Nafion film via electrostatic interaction. The obtained ECL platform (Ru(bpy) 3 2+ /AuNP/Nafion/GCE) exhibited strong ECL emission. Thiol-functionalized single-stranded DNA (ssDNA) was modified with a ferrocenyl (Fc) group and autonomously assembled on the ECL platform of Ru(bpy) 3 2+ /AuNP/Nafion/GCE via thiol-gold bonding, resulting in the quenching of ECL emission. After hybridization of the LPS aptamer strand (AS) with its partial complementary strand (CS), the formed double-stranded DNA (dsDNA) could activate CRISPR-Cas12a to indiscriminately cleave ssDNA-Fc on the surface of Ru(bpy) 3 2+ /AuNP/Nafion/GCE, resulting in recovery of the ECL intensity of Ru(bpy) 3 2+ due to the increasing distance between Fc and the electrode surface. The combination of LPS and AS suppressed the formation of dsDNA, inhibited the activation of CRISPR-Cas12a, and prevented further cleavage of ssDNA-Fc. This mechanism aided in upholding the integrity of ssDNA-Fc on the surface of the electrode and was combined with ECL quenching induced by the target. The ECL intensity decreased linearly as the concentration of LPS increased from 1 to 50,000 pg/mL and followed a logarithmic relationship. This method exhibited a remarkably low detection limit of 0.24 pg/mL, which meets the requirement for low-concentration detection of LPS in the human body. The proposed method demonstrates the capacity of CRISPR-Cas12a to perform non-specific cutting of single-stranded DNA and transform the resultant cutting substances into changes in the ECL signal. By amalgamating this approach with the distinct identification abilities of LPS and its aptamers, a simple, responsive, and discriminatory LPS assay was established that holds immense significance for clinical diagnosis.
研究领域:
生物传感
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分子诊断
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临床医学
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry A
·
2024
·Vol. 12
·期 16
·9817-9829
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IF 10.7
Liwei Hou,Xinyue Xu,Xiaoli Wang,Li Wang,Fengchun Tian,Yi Xu
In this paper, we propose a composite nano-microstructure comprising Ti3C2 and nanogold as a surface-enhanced Raman spectroscopy (SERS)-active substrate for the highly sensitive sensing of amine gases. The adsorption performance of Ti3C2 was tested by a quartz crystal microbalance (QCM) sensor. Excellent adsorption ability of Ti3C2 was observed for aniline, phenylenediamine, and diethylamine, with adsorption amounts exceeding 2210, 1740, and 970 ng cm−2, respectively. The synergistic effect of the electromagnetic properties of Ti3C2 and nano-Au in the Ti3C2/nano-Au composite nanostructures was verified by finite-difference time-domain simulation. Partial chemical enhancement between Ti3C2 and aniline was verified by photocurrent tests. To achieve assay specificity, mercaptoacetaldehyde (MA) was adopted as a recognition probe. The Schiff base reaction between aldehyde groups and amino groups produces the –CN– bond, with the Raman characteristic peak at 1635 cm−1, enabling precise amine identification with the SERS chip. To obtain the Ti3C2/nano-Au@MA SERS sensing chip, nano Au was deposited on an indium tin oxide substrate using the chronoamperometric method, followed by nano-Au aldolization modification through Au-SH interactions and the spin-coating of a Ti3C2 film layer. Due to the high affinity of Ti3C2 for amine molecules and the low SERS background signal of MA and Ti3C2, the SERS spectra could be used for ultra-sensitive detection of amines. Three amine gases, i.e. aniline, p-phenylenediamine, and diethylamine, were detected quantitatively using the intensity ratiometric method on the functionalized Ti3C2/nano-Au@MA SERS sensor chip. The detection limits were 1, 1, and 20 parts per billion, respectively. By identifying the characteristic fingerprint peaks and comparing the relative intensity of the –CN– characteristic peak, quantitative analysis of the components of aniline and p-phenylenediamine mixed gas was accomplished. These findings demonstrate that the Ti3C2/nano-Au@MA SERS sensor chip offers a promising approach for the detection of amine gas molecules and holds potential for applications in environmental monitoring, disease diagnosis, and chemical analysis.
研究领域:
材料科学
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分析化学
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Jiacheng Tao,Niu Feng,Yu Zhang,Yiping Chen,Zhen Zeng,Hong Jiang,Jimei Ma
The improper and excessive use of chloramphenicol has placed a heavy burden and adverse impact on the environment and human health. Herein, a fluorescent molecule with aggregation-induced emission characteristic is designed, synthesized, and encapsulated into polystyrene nanospheres, serving as an optical signaling probe in biosensors for ultrasensitive, rapid and accurate detection of chloramphenicol in milk. In this method, magnetic nanoparticles coupled with chloramphenicol antibody are used as immunomagnetic separation carriers. The polystyrene microspheres embedding aggregation-induced emission molecules are modified with complete antigen of chloramphenicol and used as stable and efficient signal probes. Chloramphenicol in sample and complete antigen modified-polystyrene microspheres are competitively bound to the immuno-magnetic carrier. The concentration is determined by the fluorescence intensity of the binding magnetic complex. This optical immunosensor presents an ultra-wide linear range (100 pg/mL–500 ng/mL), extraordinary sensitivity (limit of qualification was 100 pg/mL, 10-fold sensitive than enzyme-linked immunosorbent assay), and rapid detection speed (∼40 min). Accuracy is strong concordant with that of HPLC and ELISA in the test of milk samples. This strategy exhibits notable sensitivity, stability, specificity and reproducibility. The detection time is considerably shortened and the procedure is streamlined. It is a potential method for monitoring antibiotic contamination in a variety of settings, including the environment, food, and clinical circumstances.
研究领域:
分析化学
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食品科学
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2018
·
IF 4.244
Zhiyi Yao,Wenqi Ge,Mingwei Guo,Keren Xiao,Yadong Qiao,Zhong Cao,Hai-Chen Wu
We report a simple pyrene-based fluorescent probe , sodium 8-(2,4-dinitrophenoxy)pyrene-1,3,6-trisulfonate (HPTS-DNP), for the ultrasensitive and visual detection of thiophenol in 100% aqueous media. The sensing mechanism of this method is based on nucleophilic aromatic substitution on HPTS-DNP caused by thiophenol to afford a highly fluorescent product, 8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt (HPTS). The extremely low fluorescence background of HPTS-DNP and high quantum yield of HPTS ensure the superior sensing performance toward thiophenol, including rapid responses, off-on detection mode and excellent sensitivity. The detection limit is as low as 0.49 nmol L −1 according to the measurements with a spectrofluorimeter. This probe also features good selectivity (response ratio of thiophenol to interferents >30), precision (2.93%) and accuracy (102%). This approach could be applied for monitoring the thiophenol concentrations in environmental water samples. HPTS-DNP also showed good cell permeation capacity and low cellular cytotoxicity, indicating further application in bioimaging studies.
研究领域:
分析化学
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环境科学
·
生物医学
✓ 已验证
DOI
PubMed
高影响因子
ANALYST
·
2014
·Vol. 140
·期 4
·1046-1053
·
IF 3.906
Rui Ban,E. S. Abdel-Halim,Jianrong Zhang,Jun-Jie Zhu
A novel luminescence probe based on mono-6-amino-β-cyclodextrin (NH2-β-CD) functionalised gold nanoclusters (β-CD-AuNC) was designed for dopamine (DA) detection. The NH2-β-CD molecules were conjugated onto the surface of 11-mercaptoundecanoic acid capped AuNCs (11-MUA-AuNC) via a carbodiimide coupling reaction. The integrity of the β-CD cavities was preserved on the surface of AuNCs and they retained their capability for molecular DA host–guest recognition. DA could be captured by the β-CD cavities to form an inclusion complex in which the oxidised DA could quench the fluorescence of the β-CD-AuNC probe by electron transfer. The probe could be used to quantify DA in the range of 5–1000 nM with a detection limit of 2 nM. This sensitivity was 1–2 orders of magnitude higher than that in previously reported methods. Interference by both ascorbic acid (AA) and uric acid (UA) was not observed. Therefore, the β-CD-AuNC probe could be directly used to determine the DA content in biological samples without further separation. This strategy was successfully applied to a DA assay in spiked human serum samples and it exhibited remarkable accuracy, sensitivity and selectivity.
研究领域:
分析化学
·
生物医学
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2025
·
IF 6.9
Zongxu Liu,Jiaxue Song,Tao Wang,Huaiqiang Liu,Jie Chen,Yuanyuan Sun,Pinhua Zhang,Guangliang Cui
Technology development of low-concentration H 2 S sensing at low temperatures is of great significance for real-time surveillance of specific diseases and environment. In this work, a new Cu 2 O/graphene heterojunction sensor with an ideal interface is fabricated by 2D electrodeposition in-situ assembly method. The sensor is extremely selective to H 2 S and exhibits ultra-high H 2 S sensing properties at room temperature (R = 24125.785 % for 500 ppb H 2 S). Notably, the synergistic effects of barrier modulation and the sulfide reaction enable the sensor to detect H 2 S at concentrations as low as 5 ppb (R = 386 %) at 20 °C. Moreover, it exhibited good gas sensitivity even at temperatures as low as −20 °C (R = 71.26346 % for 100 ppb H 2 S). Furthermore, a sensor device was successfully manufactured for practical detection, which could be used for rapid detection of exhaled air and atmospheric monitoring. This work provides a new strategy for the design of high-sensitive and reliable room-temperature sensing materials for trace H 2 S gas detection.
研究领域:
材料科学
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环境科学
·
医学检测
✓ 已验证
产品关联
DOI
高影响因子
COMPOSITES SCIENCE AND TECHNOLOGY
·
2024
·
IF 8.3
Sijia Sun,Shuai Zhang,Mingzhu Pan
Intelligent fire warning system is a crucial protective equipment reduce fire hazards . A recent increasing concern is the development of sensitive fire warning materials that can be integrated with combustible materials to provide real-time alarms in the fire. However, current research on improving the sensitivity of fire warning sensors based on temperature-resistance response focuses on constructing continuous conductive networks. The effect of the heat transfer process is ignored, which limits the further improvement of alarm sensitivity. Here, we developed a strategy to improve thermal conductivity . Based on the chitosan (CS) conductive network in Ti 3 O 5 -based fire sensing system, aminated 2D h-BN nanosheets with high temperature resistance and ultrahigh in-plane thermal conductivity were further introduced. The h-BN improves the thermal conductivity of the fire warning coating from 1.43 W/m·K to 2.29 W/m·K. Intelligent fire warning sensors with ultra-sensitivity (1.16 s), low response temperature (170 °C), duration time (12 min) and durability (100 cycles) were successfully prepared by improving thermal conductivity to rapidly transfer heat to Ti 3 O 5 , which leads to a rapid decrease in resistance as the core mechanism. This work proposed an ingenious strategy for the construction of high fire safety and thermal conduction fireproof coatings, which revealed enticing prospects in the field of intelligent coatings.
研究领域:
材料科学
·
传感技术
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产品关联
DOI
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 12
·2906
·
IF 4.2
Hailin Wen,Miao Li,Chao-Yang Zhao,Tao Xu,Shuang Fu,Huimin Sui,Cuiyan Han
A highly sensitive, selective and recyclable histidine detection method based on magnetic Fe3O4@mTiO2(M-TiO2) nanocomposites with SERRS was developed. Mesoporous M-TiO2nanoparticles were functionalized with 4-aminothiophenol and then coupled with histidine through an azo coupling reaction in 5 min, producing the corresponding azo compound. The strong and specific SERRS response of the azo product allowed for ultrasensitive and selective detection for histidine with an M-TiO2device loaded with Ag NPs due to the molecular resonance effect and plasmonic effect of Ag NPs under a 532 nm excitation laser. The sensitivity was further enhanced with the magnetic enrichment of M-TiO2. The limit of detection (LOD) was as low as 8.00 × 10−12mol/L. The M-TiO2demonstrated applicability towards histidine determination in human urine without any sample pretreatment. Additionally, the M-TiO2device can be recycled for 3 cycles with the photodegradation of the azo product under UV irradiation due to TiO2-assisted and plasmon-enhanced photocatalysis. In summary, a multifunctional and recyclable M-TiO2device was synthesized based on azo coupling and SERRS spectroscopy for ultra-sensitive and specific histidine sensing. In addition, the proposed system demonstrated the potential for the multiplex determination of toxic compounds in the fields of food safety, industrial production and environmental protection, which benefit from the fingerprint property and universality of SERRS.
研究领域:
生物化学
·
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）