Food Bioscience
·
2025
·
IF 5.9
Zhengyu Wang,Quanhong Lv,Liangzhong Liu,Zhe Zhu
Constipation is a gastrointestinal disorder related to intestinal flora imbalance and inflammation. Numerous clinical studies have affirmed the efficacy of psyllium husk powder (PHP) in alleviating constipation; however, the efficacy and specific mechanism of PHP in alleviating constipation still lack comprehensive and in-depth discussions. In this study, we comprehensively investigated the mechanism by which round-bracted psyllium husk powder improves loperamide hydrochloride-induced constipation in mice, focusing on gut microbiota and fecal metabolic profiles while comparing the effects of PHP interventions at varying doses to polyethylene glycol 4000 bulk treatment for a comprehensive evaluation of PHP's efficacy in alleviating constipation. Our findings revealed that PHP significantly reduced defecation time and increased fecal output, weight, water content, intestinal motility, and short-chain fatty acid (SCFA) concentrations while positively influencing intestinal inflammation and promoting repair of the intestinal barrier, and higher doses of PHP demonstrate optimal efficacy. Furthermore, PHP markedly modified the microbial composition in constipated mice by enhancing the abundance of beneficial bacteria, particularly Akkermansia muciniphila . UPLC-QTOF-MS/MS analyses indicated that PHP contributed to elevated levels of Cucurbitacin B, BIX 01294 Trihydrochloride along with peptides like Asp-Gly-Lys-Ile-Leu and Val-Met-Met-Val-Arg among other potentially beneficial metabolites. KEGG pathway analysis revealed that PHP alleviates constipation by modulating metabolic pathways associated with SCFA production, beneficial microbiota colonization, and the reduction of intestinal inflammation, encompassing ABC transporters, biosynthesis of unsaturated fatty acids, and galactose metabolism etc. In conclusion, PHP can effectively relieve constipation by remodeling the structure of the gut microbiota while enhancing its associated metabolic pathways and products.
研究领域:
医学
·
微生物学
·
代谢组学
✓ 已验证
产品关联
DOI
高影响因子
Scientific Reports
·
2025
·Vol. 15
·期 1
·1-13
·
IF 3.9
Wu Dan,Niu Jingjie,Hu Jianping,Wang Hao,Kuang Haixue
This study aimed to assess the anti-hyperuricemia efficacy of Zhejiang psyllium polysaccharides (ZPP) in rats and to explore its underlying mechanism. Hyperuricemia was induced by intragastric administration of potassium oxonate, hypoxanthine, and adenine. The serum levels of uric acid (UA), creatinine (Cr), and blood urea nitrogen (BUN) were measured, and kidney pathology was examined. Serum metabolomics was employed to monitor metabolic alterations following ZPP intervention. Metagenomic analysis was conducted to investigate the impact of ZPP on the intestinal flora of hyperuricemia rats. The results showed that ZPP could significantly reduce the serum UA level in hyperuricemia rats and exhibited a certain renal protective effect. The metabolomics results indicated that ZPP regulates uric acid levels in rats with hyperuricemia and ameliorates renal pathological changes by modulating biomarkers associated with purine metabolism, amino acid metabolism, and lipid metabolism. Metagenomic research also found that ZPP could increase the relative abundance of uric acid metabolism-related probiotics, such as Limosilactobacillus reuteri and Lactobacillus murinus , thereby improving intestinal flora imbalance in rats with hyperuricemia.
研究领域:
生物化学与分子生物学
·
微生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Ding An,Wenjing Chen,Hongshan Liang,Jing Li,Peiyuan Zhou,Bin Li
The high viscosity of psyllium seed husk polysaccharide (PSHP) significantly restricts the solid-liquid ratio during the extraction process, especially in large-scale manufacturing operations. In this study, an efficient extraction method for PSHP using low-concentration ethanol at high temperatures was developed. The results demonstrated that the solid-liquid ratio for total ethanol-water extracted polysaccharide (TEP) extraction using 25 % ethanol was 8:200, which is sevenfold higher than that of the traditional hot water extraction method. The results of the structural characterization demonstrated that TEP preserved the integrity of its molecular structure and exhibited a high molecular weight of 2587 kDa. Furthermore, the conformational and rheological analyses revealed that the substantial contraction of molecular chains in the presence of ethanol resulted in a reduced viscosity of TEP across a broad temperature range, thereby ensuring high efficiency of the extraction process. Therefore, this method preserves the integrity of the TEP molecular structure while enhancing the extraction efficiency, demonstrating considerable potential for large-scale TEP production.
研究领域:
食品科学
·
天然产物化学
·
高分子材料科学
·
生物化工
·
药用植物资源开发
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Fenfen Li,Pengcheng Du,Weiyu Yang,Danfei Huang,Shaoping Nie,Mingyong Xie
The intestinal epithelium is known as an important barrier to protect the body from harmful pathogens or toxic substance that may induce intestinal barrier injury. The aim of this study was to investigate the effects of polysaccharide from the seeds of Plantago asiatica L. (PLP) on nonylphenol (NP) induced intestinal barrier injury in vitro . Caco-2 cells were pretreated with PLP, or co-cultured with PLP and NP simultaneously, and cytotoxicity, LDH leakage, transepithelial electrical resistance (TEER), FITC-dextran flux and tight junction (TJ) proteins were conducted to evaluate the intestinal barrier function. The results suggested that PLP pretreatment or co-culture with NP could significantly attenuated NP induced Caco-2 cytotoxicity, suppressed LDH release, restored the TEER value and paracellular permeability of Caco-2 monolayers, which were attributed to enhancing the TJ protein expressions. In addition, PLP co-cultured with NP possessed better protective effects against NP induced cytotoxicity. This study indicated that PLP assuaged NP induced intestinal barrier injury by increasing TJ, and threw light on the development of a dietary supplementation for preventing exogenous toxic substances induced intestinal barrier injury or improving intestinal TJ barrier function.
研究领域:
药理学
·
毒理学
·
细胞生物学
·
胃肠病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）