JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2024
·
IF 5.7
Meng-Jie Tang,Yu-Tong Ye,Zhen-Zhen Li,Mi-Zhuan Li,Pan-Pan Chen,Qi-Le Zuo,Mian Li,Zhong-Xiu Chen
The effects of metformin on invertase activity and its inhibition on sucrose digestion were studied. The rapid unfolding kinetics of invertases, followed a two-state model with an inactive intermediate formation. The dynamic interaction between metformin and invertase caused the secondary structure of the enzyme to become less β-sheet, more α-helix, and random coiling oriented, which weakened the binding force between enzyme and its substrate. Metformin acted as a chaotrope and disrupted the hydrogen bonds of water, which facilitated the unfolding of invertase. However, some sugar alcohols, which promoted the H-bond formation of water, could repair the secondary structure of metformin-denatured invertase and therefore regulate the enzyme activity. This research enriches our understanding of the mechanism of enzyme unfolding induced by guanidine compounds. Moreover, because metformin and sugar substitutes are of concern to diabetes, this research also provides useful information for understanding the activity of the digestive enzyme that coexists with metformin and sugar alcohols.
研究领域:
生物化学
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药理学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
·
2018
·
IF 2.776
Huike Dong,Guanghua Lu,Zhenhua Yan,Jianchao Liu,Matthew Nkoom,Haohan Yang
The ubiquitous existence of hexabromocyclododecane (HBCD) in environmental matrices has made it attractive to both field investigators as well as laboratory researchers. However, literature on the biological effects caused by HBCD on aquatic vertebrates seldom exist. This has inevitably increased the difficulty of toxicological assessment in the aquatic environment. Juvenile crucian carp ( Carassius carassius ) were exposed (flow-through) to different concentrations of technical HBCD (nominal 2, 20, 200 μg L −1 ) for 7 days to determine the responses of antioxidant and biotransformation enzymes. HBCD was found to be increasingly bioconcentrated in the fish livers as time proceeds. Also, the contribution of α-HBCD exhibited an enhancement from 13% in the exposure solutions to 24% in crucian carp, still much lower than in wild fishes (ca. 80%). HBCD induced activities of antioxidant enzymes in most cases, as well as increased level of lipid peroxidation. In contrast to the weak response of 7-ethoxyresorufin-O-deethylase (EROD), 7-pentoxyresorufin-O-depentylase (PROD) activity was generally induced in a time-dependent manner with peaks at day 2. Phase II enzyme Glutathione-S-transferase (GST) showed a dose-dependent induction with maximums in the 20 μg L −1 treatment at all the four timepoints of 1, 2, 4 and 7 days. Some enzymatic responses showed good associations, indicating coordinated functions. To sum up, tHBCD exposure in the present circumstance had produced an ecological stress to crucian carp. The low levels of biotransformation and slow rates of bioisomerization suggest a possible long-term toxic effect, especially around HBCD point sources.
研究领域:
环境科学
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毒理学
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水生生物学
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DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2022
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IF 6.911
Xinxin Ren,Kaiyue Hu,Lingxia Qin,Di Wu,Zhiyong Guo,Sui Wang,Yufang Hu
The investigation on invertase (INV) and glucose oxidase (GOx)-dominated biological process offers a new opportunity for the development of clinical diagnosis and prognostic treatment. Herein, a ZnO nanoflowers (ZnONFs)-assisted DNAzyme-based electrochemical platform for INV- and GOx-dominated biosensing is proposed by the change of pH in microenvironment. In this strategy, INV can usually catalyze the dissolution of sucrose to generate glucose, and glucose is then consumed by GOx to produce H 2 O 2 and gluconic acid , in which ZnONFs can be effectively etched into free Zn 2+ ions. Subsequently, the released Zn 2+ ions have a shearing action for Zn 2+ -specific DNAzyme, thus triggering hybridization chain reaction along with the imbedding of methylene blue . The excellent electrochemical signals illustrate the method can be employed well for testing sucrose, INV and GOx with a low detection limit (0.019 μM, 0.047 mU/mL and 0.012 mU/mL, respectively). Finally, a series of basic and advanced logic gates (YES, AND, INHIBIT, and AND-AND-INHIBIT) in the biological process are constructed with different logic inputs, providing a valuable platform for the establishment of advanced molecular devices for bioanalysis and clinical diagnostics.
研究领域:
生物化学
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分子生物学
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分析化学
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
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2024
·
IF 4.8
Xin-Yu Zu,Ya-Nan Zhao,Yan Liang,Ying-Qiu Li,Chen-Ying Wang,Xiang-Zhong Zhao,Hua Wang
The rapid screening of angiotensin I-converting enzyme (ACE) inhibitory peptides (ACEIPs) from protein hydrolysates remains a challenge in current researches. The current study presents the development of an efficient affinity medium (AOPAN–ACE) consisting of ACE immobilized on the surface of electrostatically spun polyacrylonitrile nanofibrous membranes for the efficient screening of ACEIPs. The application of this affinity medium followed by liquid chromatography–tandem mass spectrometry analysis resulted in the successful screening and identification of 60 potential ACEIPs from protein hydrolysates of tuna dark muscle. Notably, the application of PeptideRanker, pLM4ACE, and molecular docking approaches resulted in the screening and identification of a novel ACEIP FPPDVA. Furthermore, the peptide FPPDVA was synthesized using the solid-phase method, and its IC 50 value on ACE was determined to be 87.11 ± 1.02 μM. Molecular docking analysis suggested that the ACEIP of the peptide FPPDVA is attributable to the formation of hydrogen bonds with the S1 active site (Ala354 and Tyr523) of ACE and metal–ligand bonds with Zn 2+ . In addition, molecular dynamics simulations revealed that the peptide FPPDVA formed a stable complex with ACE and was consistently localized within the active pocket of ACE over a simulation duration of 100 ns without being displaced from the active site. Lineweaver–Burk analysis further confirmed that the peptide FPPDVA exhibited mixed mode of inhibition against ACE. Overall, the affinity medium developed in the current study is potentially useful for highly efficient screening of ACEIPs, and the novel ACEIP FPPDVA identified herein is a promising functional food ingredient for antihypertensive application.
研究领域:
食品科学
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生物化学
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药物开发
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DOI
高影响因子
Analytical Methods
·
2017
·Vol. 9
·期 46
·6507-6512
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IF 1.9
Fang Li,Ruihua Zhang,Huaibin Kang,Yonggang Hu,Yunhong Liu,Junya Zhu
Clenbuterol (CLB) is a β-agonist drug that is illegally added as a growth-promoting agent in livestock. In this study, a facile and sensitive method for the detection of CLB using a personal glucose meter (PGM) was reported. Clenbuterol–bovine serum albumin functionalized magnetic beads were used to compete with CLB for binding to its corresponding antibody and gold nanoparticle–invertase–secondary antibody conjugates. In the sucrose solution, the invertase catalyzes the hydrolysis of sucrose to produce a large amount of glucose and quantitative readout using the PGM. The ratio of signals from the PGM before and after addition of CLB exhibits a good linear correlation with the logarithm of the CLB concentrations in the range of 0.1–100 ng mL−1 with a detection limit of 0.1 ng mL−1 (S/N = 3). Finally, the proposed method was successfully applied for the detection of CLB residues in pork and liver samples.
研究领域:
分析化学
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食品安全
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生物化学
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产品关联
DOI
高影响因子
Frontiers in Bioengineering and Biotechnology
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2022
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IF 6.064
Zheng Laibao,Shen Yunqiu,Dong Wenjia,Zheng Chaochuan,Zhou Ruolan,Lou Yong-Liang
Rapid detection of pathogens and assessment of antimicrobial susceptibility is of great importance for public health, especially in resource-limiting regions. Herein, we developed a rapid, portable, and universal detection method for bacteria using AgNPs-invertase complexes and the personal glucose meter (PGM). In the presence of bacteria, the invertase could be released from AgNPs-invertase complexes where its enzyme activity of invertase was inhibited. Then, the enzyme activity of invertase was restored and could convert sucrose into glucose measured by a commercially PGM. There was a good linear relationship between PGM signal and concentration of E. coli or S. aureus as the bacteria model with high sensitivity. And our proposed biosensor was proved to be a rapid and reliable method for antimicrobial susceptibility testing within 4 h with consistent results of Minimum Inhibitory Concentrations (MICs) testing, providing a portable and convenient method to treat infected patients with correct antibiotics and reduce the production of antibiotic-resistant bacteria, especially for resource-limiting settings.
研究领域:
微生物学
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医学诊断
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生物传感器技术
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DOI
PubMed
高影响因子
ELECTROANALYSIS
·
2021
·Vol. 34
·期 2
·246-251
·
IF 3.223
Ling Li,Dong Liang,Wuhua Guo,Dianping Tang,Yongyi Zeng
Methods based on immunoassays have been developed for cancer biomarker alpha-fetoprotein (AFP), but most involve complicated or stripping procedures and are unfavourable for routine use. Herein we report the proof-of-concept of simple and point-of-care (POC) immunoassay for AFP in hepatocellular carcinoma on a portable personal glucometer (PGM) by using antibody-invertase cross-linkage nanoparticles as the signal-generation tags. Antibody-invertase cross-linkage nano tags were synthesized by using reverse micellar method with glutaraldehyde. The POC immunoassay was carried out on monoclonal anti-AFP capture antibody-coated microplate with a sandwich-type reaction mode. Introduction of invertase nano labels accompanying target AFP could hydrolyse sucrose into glucose and fructose. The produced glucose molecules could be determined on a portable personal glucometer. Relative to unimolecular invertase labelling, improved analytical features were acquired with antibody-invertase nano labelling. With the nano labels, PGM-based immunoassay exhibited good electrochemical responses for the detection of AFP, and allowed detection of AFP at a concentration as low as 5.4 pg mL −1 . Moreover, a good repeatability and intermediate precision could be found down to 12.68 %. Good well-matched results were obtained for analysis of human serum specimens between POC immunoassay and commercial human AFP ELISA kit.
研究领域:
生物技术
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医学诊断
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纳米技术
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肿瘤学
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产品关联
DOI
高影响因子
SENSORS
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2021
·Vol. 21
·期 3
·1003
·
IF 3.576
Dongmei Li,Chaofan Weng,Yi Ruan,Kan Li,Guoan Cai,Chenyao Song,Qiang Lin
A chiral sensor with optical rotation detection based on weak measurement for the kinetic study of sucrose hydrolysis is presented. Based on the polarization modulation to the pre-selection state, the optical rotation of chiral sample was accurately determined through the central wavelength shift of the output spectrum. With this approach, the concentration response curves of sucrose and its hydrolysis products, i.e., fructose and glucose, were experimentally obtained for the hydrolysis analysis. By collecting the output spectrum with a frequency of 100 Hz and fitting the central wavelength shift synchronously during the measurement, the sucrose hydrolysis process was monitored in real time. Different hydrolysis conditions with varied concentration of invertase enzyme and citrate were implemented in this work. As a consequence, the real-time hydrolysis curves of the hydrolysis process with distinct velocities was achieved and analyzed. Such a kinetic monitoring about sucrose hydrolysis with optical rotation detection technology played a critical role in the researches involving sucrose, and also revealed the great potential of weak measurement in intersections, such as food safety inspection and chemical analysis.Keywords:chiral sensor;optical sensors;weak measurement;sensor systems and applications;sucrose hydrolysis
研究领域:
光学传感器
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化学分析
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食品安全检测
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生物技术
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING & TECHNOLOGY
·
2023
·Vol. 46
·期 5
·858-864
·
IF 2.1
Yu Liu,Yu-Tong Li,Ling Liu,Shu Bai,Lin-Ling Yu,Yan Sun
Polyethylenimine (PEI)-grafted silica nanoparticles were fabricated and characterized for the electrostatic immobilization of a CO 2 conversion enzyme. PEI was supposed to serve as a carbonic anhydrase mimic for CO 2 capture and conversion to HCO 3 − (the optimal substrate of many CO 2 conversion enzymes) and was proved to assist phosphoenolpyruvate carboxylase to form a dual-enzyme cascade system for CO 2 conversion. The immobilized enzyme not only presented better thermostability, pH tolerance, and storage stability, but also enhanced the specific activity (three- and fourfold for HCO 3 − and CO 2 as the substrates, respectively), with good reusability and low cost. It was proven that PEI-grafted nanoparticles are highly efficient nanocarriers for immobilizing CO 2 -converting enzymes in industrial applications.
研究领域:
生物化学
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纳米生物学
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环境科学
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产品关联
DOI
高影响因子
Pharmaceutics
·
2023
·Vol. 15
·期 10
·2377
·
IF 5.4
Hua Cai,Rongrong Zheng,Ningxia Wu,Jiaman Hu,Ruixin Wang,Jianing Chi,Wei Zhang,Linping Zhao,Hong Cheng,Ali Chen,Shiying Li,Lin Xu
Chemotherapy-induced side effects restrain anti-tumor efficiency, with hyperlipidemia being the most common accompanying disease to cause treatment failure. In this work, a chimeric peptide-engineered nanomedicine (designated as PRS) was fabricated for the synergistic suppression of tumor growth and therapy-induced hyperlipidemia. Within this nanomedicine, the tumor matrix-targeting peptide palmitic-K(palmitic)CREKA can self-assemble into a nano-micelle to encapsulate Rapamycin (mTOR inhibitor) and SBC-115076 (PCSK9 inhibitor). This PRS nanomedicine exhibits a uniform nano-distribution with good stability which enhances intracellular drug delivery and tumor-targeting delivery. Also, PRS was found to synergistically inhibit tumor cell proliferation by interrupting the mTOR pathway and reducing Rapamycin-induced hyperlipidemia by increasing the production of LDLR. In vitro and in vivo results demonstrate the superiority of PRS for systematic suppression of tumor growth and the reduction of hyperlipidemia without initiating any other toxic side effects. This work proposes a sophisticated strategy to inhibit tumor growth and also provides new insights for cooperative management of chemotherapy-induced side effects.Keywords:anti-tumor;hyperlipidemia;tumor-targeted peptide;nanomedicine;Rapamycin;proprotein convertase subtilisin/kexin type 9 (PCSK9)
研究领域:
肿瘤学
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药理学
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纳米医学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Yixin Li,Zitong Wang,Jiaqi Wu,Jie Zheng,Fu Liu,Juanying Ou,Caihuan Huang,Shiyi Ou
Reactive carbonyl species can modify digestive enzymes upon intake due to their electrophilic nature. This study evaluated the effects of methylglyoxal (MGO), glyoxal, acrolein, and formaldehyde on invertase, an enzyme presents in digestive tract. Unexpectedly, MGO enhanced, rather than inhibited, invertase activity. Moreover, MGO counteracted the inhibitory effects of the other three carbonyls on invertase activity. Kinetic analyses revealed that 150 mmolLexp.-1 MGO resulted in a 2-fold increase in the Km and a 3.3-fold increase in Vmax, indicating that MGO increased the turnover rate of sucrose while reducing the substrate binding affinity of invertase. Additionally, MGO induced dynamic quenching of fluorescence, reduced free amino groups, increased hydrophobicity, the content of Amadori products, fluorescent and nonfluorescent AGEs, and amyloid fibrils of invertase. The specific modifications responsible for the elevated activity of MGO on invertase require further investigation.
研究领域:
生物化学
·
酶学
·
食品科学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Wen Yin,Ji Hu,Fang Chen,Li Zhu,Yingxin Ma,Nuo Wang,Hongping Wei,Hang Yang,Shan-Ho Chou,Jin He
Background COVID-19 (coronavirus disease 2019) pandemic has had enormous social and economic impacts so far. The nucleocapsid protein (N protein) is highly conserved and is a key antigenic marker for the diagnosis of early SARS-CoV-2 infection. Results In this study, the N protein was first captured by an aptamer (Aptamer 58) coupled to magnetic beads (MBs), which in turn were bound to another DNA sequence containing the aptamer (Aptamer 48-Initiator). After adding 5′-biotinylated hairpin DNA Amplifier 1 and Amplifier 2 with cohesive ends for complementary hybridization, the Initiator in the Aptamer 48-Initiator began to trigger the hybridization chain reaction (HCR), generating multiple biotin-labeled DNA concatamers. When incubated with synthetic streptavidin-invertase-Ca 3 (PO 4 ) 2 hybrid nanoflower (SICa), DNA concatamers could specifically bind to SICa through biotin-streptavidin interaction with high affinity. After adding sucrose, invertase in SICa hydrolyzed sucrose to glucose, whose concentration could be directly read with a portable glucometer, and its concentration was positively correlated with the amount of captured N protein. The method is highly sensitive with a detection limit as low as 1 pg/mL. Significance We believe this study provided a practical solution for the early detection of SARS-CoV-2 infection, and offered a new method for detecting other viruses through different target proteins.
研究领域:
生物技术
·
分子生物学
·
病毒学
·
分析化学
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DOI
PubMed
高影响因子
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