INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2021
·Vol. 23
·期 1
·101
·
IF 5.924
Jianfa Cai,Xuelian Mo,Chenjin Wen,Zhen Gao,Xu Chen,Cheng Xue
Strawberry is a soft fruit with short postharvest life, due to a rapid loss of firmness. Pectin methylesterase (PME)-mediated cell wall remodeling is important to determine fruit firmness and softening. Previously, we have verified the essential role ofFvPME38in regulation of PME-mediated strawberry fruit softening. However, the regulatory network involved in PME-mediated fruit softening is still largely unknown. Here, we identified an R2R3-type MYB transcription factor FvMYB79, which activates the expression level ofFvPME38, thereby accelerating fruit softening. During fruit development,FvMYB79co-expressed withFvPME38, and this co-expression pattern was opposite to the change of fruit firmness in the fruit of ‘Ruegen’ which significantly decreased during fruit developmental stages and suddenly became very low after the color turning stage. Via transient transformation, FvMYB79 could significantly increase the transcriptional level ofFvPME38, leading to a decrease of firmness and acceleration of fruit ripening. In addition, silencing ofFvMYB79showed an insensitivity to ABA-induced fruit ripening, suggesting a possible involvement ofFvMYB79in the ABA-dependent fruit softening process. Our findings suggest FvMYB79 acts as a novel regulator during strawberry ripening via transcriptional activation ofFvPME38, which provides a novel mechanism for improvement of strawberry fruit firmness.Keywords:fruit softening;strawberry;FvMYB79;FvPME38
研究领域:
植物生物学
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分子遗传学
·
农学
✓ 已验证
DOI
PubMed
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2013
·
IF 2.454
Jianwen Bu,Yucong Yu,Guzhanuer Aisikaer,Tiejin Ying
Mature green cherry tomato fruit were harvested and treated with ultraviolet-C (UV-C) irradiation at a predetermined dose of 4.2 kJ m −2 and stored at 18 °C for 35 days. The effects of UV-C treatment on firmness, cell wall compositions (pectin and cellulose), cell wall ultra-structure, levels of ethylene production, and activities of cell wall degrading enzymes in the fruit were investigated during storage. Major genes involved in cell wall degradation ( PME 2.1, Cel 1, PG cat and Exp 1) were also checked. Fruit firmness was better maintained in UV-C treated fruit corresponding with higher contents of cellulose and acid-soluble pectin. Transmission electron microscopy indicated that UV-C irradiation retarded cell wall disassembly in the pericarp. Ethylene production was significantly inhibited by UV-C treatment. The UV-C treatment also suppressed the transcriptional expression of PME2.1 , Cel1 , PGcat and Exp1 genes, and inhibited pectin methylesterase (PME; EC 3.1.1.11), polygalacturonase (PG; EC3.2.1.15) and cellulase (EC3.2.1.4) activities during storage. These results suggested that the inhibition of ethylene production, which in turn down-regulated expression of genes encoding cell wall degrading enzymes, might be one of the possible mechanisms of UV-C delaying tomato fruit softening.
研究领域:
植物学
·
园艺学
✓ 已验证
DOI
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2018
·
IF 3.141
Yang Yu,Huachang Jin,Xindi Jin,Runxin Yan,Li Zhang,Xueming Chen
Electrochemical precipitation has obtained great attentions for water softening in recent years. In order to avoid cathode deactivation, periodical scale detachment is essential. In general, the scale adhered to the cathode is detached by mechanical scraping. Although mechanical scraping is easy operated, elastic scraper must be installed between electrodes, leading to the low softening efficiency and high energy consumption. To overcome such a problem, a new current pulsated electrochemical precipitation process was proposed in this paper. Scale detachment was accomplished by increasing the current density significantly. Experimental results showed that the both high softening and detachment performance were achieved. The precipitation rate was as high as 40.47 g/h/m2. The energy consumption and the total hardness removal efficiency were 8.9–13.2 kWh/kg CaCO3 and 17.8–22.8%, respectively. Repetitive experimental results indicated the current pulsated electrochemical precipitation process could run steadily without any performance decay detected after repetitive operation.
研究领域:
环境科学
·
水处理工程
✓ 已验证
产品关联
DOI
高影响因子
EUROPEAN JOURNAL OF INORGANIC CHEMISTRY
·
2016
·
IF 2.686
Yanjie Wang,Kiyoshi Nomura,Xin Liu,Alexandre I. Rykov,Changzi Jin,Tao Liu,Junhu Wang
Graphical Room-temperature ferromagnetism (RTFM) was found in 57 Fe-TiO 2 and 57 Fe/Sn-TiO 2 materials prepared by a soft-chemical solution method. Their magnetic properties were found to decrease with increasing degree of 57 Fe doping. Fe 3+ –oxygen vacancy (V O )–Fe 3+ centers are responsible for the observed RTFM. A series of 57 Fe-doped TiO 2 and 57 Fe/Sn-codoped TiO 2 materials were prepared by a soft-chemical solution process with various 57 Fe concentrations from 0.1 to 1.0 at.-% and a fixed Sn concentration of 2.0 at.-%. In this process, an aqueous H 2 O 2 solution reacts with iron and titanium or iron, tin, and titanium precursor compounds to produce peroxopolymetallic acids. The structures and magnetic properties of the products were characterized by magnetic-moment measurements, X-ray diffraction, and Raman and Mössbauer spectroscopy. The results showed that all samples consisted of a rutile phase (and a trace amount of anatase in 57 Fe-TiO 2 ). All samples exhibited hysteresis loops in the M – H curves, which demonstrated the presence of room-temperature ferromagnetism. In addition, their magnetic properties decreased with increasing degree of 57 Fe doping. Oxygen vacancies were considered to be responsible for the presence of ferromagnetism.
研究领域:
材料科学
·
物理学
✓ 已验证
产品关联
DOI
高影响因子
CERAMICS INTERNATIONAL
·
2020
·
IF 3.83
Kewei Wang,Baisheng Ma,Tao Li,Chunxiao Xie,Zhenzhong Sun,Dianguang Liu,Jinling Liu,Linan An
In this study, powder mixtures at atomic ratios suitable for formation of Sr(Ti 0.2 Y 0.2 Zr 0.2 Sn 0.2 Hf 0.2 )O 3-x were flash sintered at 1000 °C and 40 mA/mm 2 . X-ray diffraction and energy-dispersive spectroscopy analyses revealed the formation of single-phase oxides after sintering for 1 min. This rapid formation of single-phase oxide was attributed to flash sintering characteristics, which not only enhanced the diffusion rate, but also softened the lattice. Compared to conventional heat treatment sintering, the sintering time was shortened to 1 min from 10 h, while the sample temperature was reduced to 1314 from 1500 °C. Therefore, flash sintering is a promising technique for rapid synthesis of high-entropy oxides.
研究领域:
材料科学
·
陶瓷工程
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Qianqian Tang,Hao Wu,Mingsong Zhou,Dongjie Yang
Presently, most studies on modified lignin focused on the adsorption to heavy metal cations, but rarely to Ca 2+ in hard water . Therefore, this work prepared a new gel-type lignin-based cationic adsorption resin (E-LSAF) through the crosslinking and curing of alkali lignin grafted by sodium sulfite sulfonated acetone to remove Ca 2+ in water. Under the determined optimum synthesis conditions, E-LSAF with a highest sulfonic group content of 1.99 mmol/g was obtained. Structural and physicochemical measuring results showed E-LSAF was a gel-type resin, owning strong hydrophilicity , high mechanical strength, excellent thermal stability and acid-alkaline resistance. Adsorption results indicated the adsorption of E-LSAF to Ca 2+ was well-fitted by Langmuir model, and the maximum adsorption capacity reached 45.8 mg/g. Pseudo-second-order model can describe this adsorption process well, suggesting it a chemisorption process. Dynamic column adsorption results showed E-LSAF could transform hard water into soft or even very soft water. The regeneration efficiency still maintained 80 % after 5 cycles. The adsorption mechanism was attributed to electrostatic attraction, ion exchange and complexation. This work provided a high-performance lignin-based cationic adsorption material with high adsorption capacity to Ca 2+ and excellent acid-alkaline resistance, which filled the research gap of using modified sulfonated lignin to remove Ca 2+ from water.
研究领域:
材料科学
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环境科学
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2021
·Vol. 102
·期 5
·1919-1926
·
IF 3.639
Jiandong Shen,Qixing Jiang,Wei Zhang,Yanshun Xu,Wenshui Xia
BACKGROUND Texture softening is always a problem during chilling of grass carp fillets. To solve this problem and provide for better quality of flesh, understanding the mechanism of softening is necessary. Gelatinolytic proteinases are suspected to play an essential role in the disintegration of collagen in softening of fish flesh. In the present study, the types and contribution of gelatinolytic proteinases in chilled fillets were investigated. RESULTS Four active bands (G1, 250 kDa; G2, 68 kDa; G3, 66 kDa; G4, 29 kDa) of gelatinolytic proteinases were identified in grass carp fillets by gelatin zymography. The effect of inhibitors and metal ions revealed that G1 was possibly a serine proteinase, G2 and G3 were calcium-dependent metalloproteinases and G4 was a cysteine proteinase. The effect of the inhibitors phenylmethanesulfonyl fluoride (PMSF), l -3-carboxy- trans -2,3-epoxy-propionyl- l -leucine-4-guanidinobutylamide (E-64) and 1,10-phenanthroline (Phen) on chilled fillets revealed that gelatinolytic proteinase activities were significantly suppressed. Collagen solubility indicated that metalloproteinase and serine proteinase played critical roles in collagen breakdown during the first 3 days, and cysteine proteinase revealed its effect after 3 days. Meanwhile, during chilled storage for 11 days, the final values of shear force increased 19.68% and 24.33% in PMSF and E-64 treatments when compared to control fillets respectively, whereas the increase after Phen treatment was 49.89%. CONCLUSION Our study concluded that the disintegration of collagen in post-mortem softening of grass carp fillets was mainly mediated by metalloproteinase and to a lesser extent by serine proteinase and cysteine proteinase. © 2021 Society of Chemical Industry.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
COMPOSITES SCIENCE AND TECHNOLOGY
·
2024
·
IF 8.3
Qiao Li,Hongda Meng,Yihu Song,Qiang Zheng
Silica is an important filler of “green tires” while its dispersion in the aid of silane coupling agents emits volatile organic compounds during rubber compounding and its invariably agglomeration in nonpolar rubber matrices enhances strain softening . Herein a highly active deep eutectic solvent (DES), synthesized using stearic acid as hydrogen bond donor and tetrabutylammonium chloride as hydrogen bond acceptor, is used to tailor reinforcement and softening behaviors and to replace the silane coupling agents for preparing volatile organic compounds-free nanocomposites . The results show that DES can regulate the crosslinking network structure of rubber matrix and accelerate the vulcanization by reacting with non-rubber components in natural rubber (NR) and by improving the proportion of disulfidic linkage. Furthermore, DES is able to improve the dispersion of silica, crosslinking density of NR and the interfacial interaction between silica and NR, and slow down the thermo-oxidative aging behavior. It could also weaken the damping and softening accompanying Mullins effect for the nanocomposites vulcanizates at high strains. In comparison with silane , DES endows the nanocomposites with superior vulcanization and mechanical properties, providing guides to mediate the reinforcement and strain softening behaviors and manufacture high-performance “green tires” in an energy-efficient approach.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
Wood Material Science & Engineering
·
2024
·
IF 2.2
Mengyao Chen,Haiqiao Zhang,Xingzhou Yao,Ke Tong,Yan Wu
Deep eutectic solvents (DES) offer advantages such as environmental friendliness, recyclability, and biocompatibility, demonstrating promising applications in functional materials, chemical reactions, and electrochemistry. This study investigated the softening effect of DES on beech with the assistance of FeCl3, considering different treatment durations (1, 2, 3, and 4 h) on varying thicknesses (1, 2.5, 3.5, and 5 mm). Results indicate that longer treatment time leads to better softening effects on beech wood at the same thickness. FeCl3 acts as an active site providing acidic protons, thereby enhancing the softening efficiency of DES. Wood treated with Fe3+-assisted DES removes 86% of the lignin and 48% of the hemicelluloses, resulting in enhanced flexibility. Scanning electron microscopy reveals that most of the vessel lumens, cell lumens, and intercellular spaces of beech wood treated with Fe3+-assisted DES are filled with DES, reducing the wood's porosity. Color difference analysis and energy-dispersive X-ray spectroscopy demonstrate that wood color darkens gradually with prolonged treatment time, correlating with the addition of FeCl3. Thermal analysis indicates an improvement in the flame retardancy of the treated wood. This research methodology significantly enhances wood utilization and diversifies the aesthetic possibilities of wood products, thereby may expanding their applications in architecture and furniture.
研究领域:
材料科学
·
林业科学
✓ 已验证
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Jiayu Luo,Zhengshuo Zhan,Weiquan Li,Yang Lei
Electrochemical water softening, known for its chemical-free operation and modular design, offers a sustainable solution for cooling water management. However, the impact of antiscalants on the efficiency of electrochemical water softening remains poorly understood. Here we showed that even low concentrations (7.5 µM) of nitrilotris methylene phosphonic acid (NTMP), a representative antiscalant, significantly inhibit electrochemical softening efficiency while substantially increasing energy consumption. We propose a dual-benefit strategy integrating adsorption with electrochemically mediated precipitation to address this challenge in treating antiscalants-loaded cooling water. First, we probed the efficiency of iron-based adsorbents in mitigating NTMP under cooling water-relevant conditions. Our results show that bicarbonate hinders NTMP adsorption by altering the solution pH and the surface charge of the adsorbents. In contrast, hardness ions (Ca 2+ and Mg 2+ ) enhance NTMP adsorption by forming ternary complexes. Following adsorption, the subsequent electrochemical treatment achieves 83% calcium hardness removal at 20 A/m 2 with an energy consumption of 11.1 kWh/kg CaCO 3 in synthetic solutions. Notably, NTMP molecules desorbed from iron-based adsorbents retain their full anti-scaling capacity (100%), enabling recovery and reuse. This dual-function strategy mitigates antiscalant inhibition, enhances softening efficiency, and enables antiscalant recovery for reuse, advancing sustainable water treatment.
研究领域:
环境科学
·
水处理技术
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 96
·93947-93955
·
IF 3.289
Bin Dong,Ying Xu,Danni Shen,Xiaohu Dai,Senming Lin
Reusing wastewater can enormously reduce environmental pollution and save water. One of the key obstacles to reuse is the scaling caused by calcium (Ca) ions and humic matter (HM). The interactions among HM, Ca ions and cation-exchange resins (CERs) during the water softening process were explored via lab-scale and pilot-scale tests. Surface topography, pore structure, and surface molecular structure of the CERs were determined using an atomic-force microscope, an automatic mercury porosimeter, a laser-Raman spectrum and X-ray photoelectron spectroscopy, respectively. The surface charge of CERs was assessed by measuring zeta potential. Experimental results showed that HM can react with Ca-ions to form HM–Ca+ and Ca ions can act as a bridge for HM and CER, suggesting that Ca ions and HM can be simultaneously removed by CERs. Kinetic analyses indicate that a pseudo-first order equation was able to accurately describe the overall adsorption kinetics and diffusion of Ca ions through a liquid film of CER was the rate-determining step for the overall adsorption process. Further investigation revealed that during the first 60 minutes of adsorption, the effect of HM on the CER adsorption kinetics can be described well by pseudo-first and pseudo-second order equations. This indicates that diffusions of Ca ions, HM, and HM–Ca+ and their interactions were equivalent for controlling the initial stage of adsorption. In addition, 99% of Ca ions and 30–60% of HM in wastewater were removed in the pilot-scale test, suggesting that CERs have significant potential to remove Ca ions and HM simultaneously in wastewater.
研究领域:
环境科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
Gels
·
2024
·Vol. 10
·期 2
·118
·
IF 5
Zhi Li,Shengjie Yao,Guichao Wang,Xi Deng,Fang Zhou,Xiaoxu Wu,Qiong Liu
The propensity of foamed concrete to absorb water results in a consequential degradation of its performance attributes. Addressing this issue, the integration of aerogels presents a viable solution; however, their direct incorporation has been observed to compromise mechanical properties, attributable to the effects of the interface transition zone. This study explores the incorporation of MTES-based aerogels into foamed cement via an impregnation technique, examining variations in water–cement ratios. A comprehensive analysis was conducted, evaluating the influences of MTES-based aerogels on the thermal conductivity, compressive strength, density, chemical composition, and microstructure of the resultant composites across different water–cement ratios. Our findings elucidate that an increment in the water–cement ratio engenders a gradual regularization of the pore structure in foamed concrete, culminating in augmented porosity and diminished density. Notably, aerogel-enhanced foamed concrete (AEFC) exhibited a significant reduction in water absorption, quantified at 86% lower than its conventional foamed concrete (FC) counterpart. Furthermore, the softening coefficient of AEFC was observed to surpass 0.75, with peak values reaching approximately 0.9. These results substantiate that the impregnation of MTES-based aerogels into cementitious materials not only circumvents the decline in strength but also bolsters their hydrophobicity and water resistance, indirectly enhancing the serviceability and longevity of foamed concrete. In light of these findings, the impregnation method manifests promising potential for broadening the applications of aerogels in cement-based materials.
研究领域:
建筑材料学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）