Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Qiang Liu,Mahmoud Mazarji,Yeqing Li,Lu Feng,Hongjun Zhou,Quan Xu,Alastair James Ward,Yajing Wang,Jianping Su,Chunming Xu,Junting Pan
Humic acid (HA) is an inhibitor that can diminish bioconversion during anaerobic digestion (AD). In this study, a non-metallic nanomaterial, N-doped carbon quantum dots (NCQD), was synthesized to alleviate the HA inhibition, followed by exploring its mechanism. Adding 500 mg/L NCQD prevented HA inhibition by binding to HA or microorganism surfaces, thus avoiding contact between them. This phenomenon, known as shielding inhibition, involves the formation of hydrogen bonds and chemical bonds. The intermolecular force and dissociation constant ( K D ) between NCQD and HA were determined to be 112.83 ± 3.98 nN and 1.0 ± 0.07 × 10 -7 M, respectively. NCQD could promote electron transfer in AD systems. According to the in-depth microbial analysis, NCQD significantly enhanced the metabolic pathways of methanogenesis and biosynthesis of coenzyme F 420 . This groundbreaking research demonstrates the dual functionality of NCQD by effectively shielding against HA inhibition and promoting electron transfer. The study also unravels the mechanism of interface interaction, electron transfer, and metabolic pathways, leading to significant breakthroughs in addressing HA inhibition.
研究领域:
环境科学
·
纳米技术
·
生物化学
·
微生物学
·
能源工程
✓ 已验证
产品关联
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2021
·
IF 9.642
Pingbo Wang,Meiying Ye,Yue Cui,Xiong Xiao,Dexun Zou,Rongxin Guo,Yanping Liu
Two-phase high-solid digestion is conducive to the degradation of food waste. In this study, Fe/C was added in high-solid digestion in different acidification and/or methanogenic phase. The experimental results indicated that it significantly increased the cumulative yield of biomethane. When Fe/C was added to the acidification phase only and both the acidification and methanogenic phases, the biomethane yield reached 474.07 ± 7.03 and 475.47 ± 4.68 mL·g VS −1 , respectively, and the biodegradation rate reached 87.30% and 87.58%, respectively, indicating that Fe/C had mainly effect on the performance of acidification phase. In a two-phase anaerobic fermentation system, the activity of dehydrogenases and the concentration of coenzyme F 420 were 2.23–2.95 mg·g −1 ·h −1 and 0.0063–0.0294 mol·g −1 volatile solids, respectively. Additionally, the archaeal communities production pathway of methane from using acetic acid to using hydrogen as the reactant.
研究领域:
环境工程
·
微生物学
·
废物管理
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ENVIRONMENTAL MANAGEMENT
·
2024
·
IF 8
Ai-Hua Li,Bao-Cai Zhang,Zhang-Wei He,Cong-Cong Tang,Ai-Juan Zhou,Yong-Xiang Ren,Zhihua Li,Aijie Wang,Wenzong Liu
Biochar has been used to enhance methane generation from anaerobic digestion through establishing direct interspecific electron transfer between microorganisms. However, the microbial communication is still inadequate, thereby limiting further methane production improvement contributed by biochar. This study investigated the roles of quorum-sensing molecules, acylated homoserine lactone (AHL), in anaerobic digestion of waste activated sludge aided by biochar. Results showed that the co-addition of separated biochar and AHL achieved best methane production performance, with the maximal methane yield of 154.7 mL/g volatile suspended solids, which increased by 51.9%, 47.2%, 17.9%, and 39.4% respectively compared to that of control, AHL-loaded biochar, sole AHL, and sole biochar groups. The reason was that the co-addition of separated biochar and AHL promoted the stages of hydrolysis and acidification, promoting the conversion of organic matters and short-chain fatty acids, and optimizing the accumulation of acetate acid. Moreover, the methanogenesis stage also performed best among experimental groups. Correspondingly, the highest activities of electron transfer and coenzyme F420 were obtained, with increase ratios of 33.2% and 27.2% respectively compared to that of control. Furthermore, biochar did more significant effects on the evolution of microbial communities than AHL, and the direct interspecific electron transfer between fermentative bacteria and methanogens were possibly promoted.
研究领域:
环境工程
·
微生物学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
·
2018
·Vol. 94
·期 3
·730-738
·
IF 2.587
Fan Yang,Jing Wang,Chen Gu,Ying Han,Guang-fei Liu,Hong Lu
BACKGROUND To accelerate direct interspecies electron transfer (DIET), magnetite is supplemented in anaerobic activated sludge (AS). However, it is usually reduced by dissimilatory iron reducing bacteria (DIRB). Therefore, an AS system supplemented with submicron magnetite particles (Fe 3 O 4 /AS) in an up-flow microaerobic sludge reactor was established, aiming to remove tribromophenol (TBP) efficiently and alleviate the magnetite dissolution. RESULTS Under microaerobic condition (DO, 0.6 mg L −1 ), the efficiencies of removal of COD, TBP, and methane production in Fe 3 O 4 /AS system were, respectively, 14.3%, 16.7%, and 27.2% higher than those of the AS system. Correspondingly, the dehydrogenase (DHA) and Coenzyme F 420 (CoF 420 ) activities were separately 1.38-fold and 1.41-fold enhanced. The enhanced biodegradation was via potential DIET, as visualized by microbes and magnetite particles, Geobacter enrichment and c -type cytochromes ( c -Cyts) increasement. Notably, magnetite majorly maintained an intact structure. In effluents, the Fe(II) concentrations were maintained at less than 10 mg L −1 under microaerobic conditions, far less than the amount under anaerobic conditions, which might be due to the in situ redox cycle of Fe(III)-reduction and Fe(II)-reoxidation. CONCLUSION Significant enhancements in removal efficiencies of TBP and COD and methanogenesis were demonstrated in a Fe 3 O 4 /AS system, indicating intrinsically faster electron transfer via electrical conduction. Putative oxidation of dissolved Fe(II) in situ occurred in microaerobic environments, which could protect magnetite in the system without extra magnetite supplementation. © 2018 Society of Chemical Industry
研究领域:
环境工程
·
生物技术
·
微生物学
·
废水处理
✓ 已验证
产品关联
DOI
高影响因子
RENEWABLE ENERGY
·
2021
·
IF 8.001
Wenjing Tian,Jianhao Li,Lirong Zhu,Wen Li,Linyan He,Li Gu,Rui Deng,Dezhi Shi,Hongxiang Chai,Meng Gao
Recent investigations verified CaO 2 accelerated hydrolysis of lignocellulose, but its residue caused a harmful oxidizing environment. This study therefore proposed the combined CaO 2 pretreatment and zero-valent iron (ZVI) addition to enhance high-solid anaerobic digestion of wheat straw, trying to eliminate negative effect of residual oxides . Effects of CaO 2 pretreatment without/with ZVI addition on digestion performance were investigated. Results showed that in non-ZVI added digesters, as CaO 2 dosage increased, total production of SCOD and VFAs increased linearly, while activities of coenzyme F 420 and electron transport system were negatively correlated. Pretreatment using appropriate CaO 2 dosage indirectly increased biogas production by promoting hydrolysis and acidification. Comparably, initial ORP in ZVI-added digesters sharply declined below −350 mV, which was suitable for methanogen growth. ZVI significantly improved activities of cellulase , hemicellulase , acetic kinase and coenzyme F 420 , with the highest improvement percentages of 138%, 48%, 35% and 58%, respectively. Since ZVI could provide electrons for conversion of CO 2 to CH 4 , compared with non-ZVI added digesters (R0-R4), methane content in ZVI-added digesters (RZ0-RZ4) increased by 8.1%, 9.0%, 11.2%, 17.0% and 18.0% successively. Consequently, 0.6 g/g VS CaO 2 pretreatment combined with 0.4 g/g VS ZVI addition achieved the maximum methane production, increasing by 35.6% compared to control digester.
研究领域:
环境科学
·
生物化学工程
·
可再生能源
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）