JOURNAL OF CELLULAR PHYSIOLOGY
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2017
·Vol. 233
·期 9
·6714-6721
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IF 4.08
Haihong Zhou,Congli Jin,Lili Cui,Huaijie Xing,Jun Liu,Wang Liao,Haojie Liao,Yangsheng Yu
This study aimed to investigate whether HMGB1 (high mobility group box-1 protein) and receptor for advanced glycation end products (RAGE) were involved in the irradiation-induced endothelial barrier damage and their mechanism. We constructed the damage model of endothelium barrier model with bEnd.3 cells. The permeability of endothelial barrier was detected by sodium fluorescein (Na-F) permeation test, and the irradiation dose which could induce permeability transition was determined by being exposed to different irradiation doses (5, 10, 15, 20 Gy). MTT assay was applied to detect cell viability under different concentrations of HMGB1 , glycyrrhizic acid (GA, a specific inhibitor of HMGB1 ), and FPS-ZM1 (a blood-brain-barrier permeant blocker of RAGE V domain-mediated ligand binding). The expression of HMGB1 , RAGE, and related molecules involved in MAPK signaling pathway, MMP-2, MMP-9, ZO-1, and claudin 5 of differently treated groups were measured by qRT-PCR, western blot, and immunofluorescence. Cells possessed stable endothelial barrier function on 4–7 days after seeded on transwell plates. The permeability of endothelial barrier would change under at least 10 Gy radiation. Both radiation and HMGB1 treatment alone could improve the permeability. After irradiation, the expressions of HMGB1 and RAGE increased and MAPK signal pathway was activated. Meanwhile, MMP-2 and MMP-9 were overexpressed, while the expression of tight junction proteins ZO-1 and claudin 5 was decreased. Radiation could activate MAPK signaling pathway through promoting the expression of HMGB1 and RAGE, which further led to endothelial barrier injury and changed its permeability.
研究领域:
细胞生物学
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分子生物学
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医学研究
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DOI
PubMed
高影响因子
Materials
·
2021
·Vol. 14
·期 2
·257
·
IF 3.623
Xiaodong Zhang,Fei Chen,Zhimin Su,Taiping Xie
The effects of electron beam irradiation on ethylene-tetrafluoroethylene copolymer (ETFE) were studied. Samples were irradiated in air at room temperature by a universal electron beam accelerator for various doses. The effect of irradiation on samples and the cross-linked ETFE after aging were investigated with respect to thermal characteristics, crystallinity, mechanical properties, and volume resistivity using thermo-gravimetric analysis (TGA), differential scanning calorimeter (DSC), universal mechanical tester, and high resistance meter. TGA showed that thermal stability of irradiated ETFE is considerably lower than that of unirradiated ETFE. DSC indicates that crystallinity is altered greatly by cross-link. The analysis of mechanical properties, fracture surface morphology, visco-elastic properties and volume resistivity certify radiation-induced cross-linking is vital to aging properties.Keywords:ETFE;irradiation;aging;mechanical properties;crystallization kinetics
研究领域:
材料科学
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高分子化学
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DOI
PubMed
高影响因子
Materials
·
2022
·Vol. 15
·期 19
·6553
·
IF 3.748
Yuan Zhao,Tian Liang,Pengpai Miao,Tao Chen,Xiaobing Han,Guowen Hu,Jie Gao
The modification of polymers can significantly improve the ability to remove rare earth ions from wastewater, but so far few studies have focused on the irradiation-induced grafting method. In this study, a novel magnetic chelating resin for Ce(III) uptake was first synthesized by suspension polymerization of PMMA@Fe3O4microspheres followed by irradiation-induced grafting of glycidyl methacrylate (GMA) and subsequent amination with polyethyleneimine (PEI). The FT-IR, SEM, TG and XRD characterization confirmed that we had successfully fabricated magnetic PMMA-PGMA-PEI microspheres with a well-defined structure and good thermal stability. The obtained adsorbent exhibited a satisfactory uptake capacity of 189.81 mg/g for Ce(III) at 318.15 K and an initial pH = 6.0. Additionally, the impact of the absorbed dose and GMA monomer concentration, pH, adsorbent dosage, contact time and initial concentration were thoroughly examined. The pseudo-second order and Langmuir models were able to describe the kinetics and isotherms of the adsorption process well. In addition, the thermodynamic data indicated that the uptake process was spontaneous and endothermic. Altogether, this research enriched the Ce(III) trapping agent and provided a new method for the removal rare earth pollutants.Keywords:magnetic PMMA microspheres;PEI;radiation induced grafting;Ce(III) adsorption
研究领域:
材料科学
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环境科学
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DOI
PubMed
高影响因子
Dose-Response
·
2019
·
IF 2.451
Yiying Gao,Xugang Li,Jingjing Gao,Zhen Zhang,Yang Feng,Jihua Nie,Wei Zhu,Shuyu Zhang,Jianping Cao
Radiation-induced lung injury is a major dose-limiting toxicity that occurs due to thoracic radiotherapy. Metabolomics is a powerful quantitative measurement of low-molecular-weight metabolites in response to environmental disturbances. However, the metabolomic profiles of radiation-induced lung injury have not been reported yet. In this study, male Sprague-Dawley rats were subjected to a single dose of 10 or 20 Gy irradiation to the right lung. One week after radiation, the obvious morphological alteration of lung tissues after radiation was observed by hematoxylin and eosin staining through a transmission electron microscope. We then analyzed the metabolites and related pathways of radiation-induced lung injury by gas chromatography–mass spectrometry, and a total of 453 metabolites were identified. Compared to the nonirradiated left lung, 19 metabolites (8 upregulated and 11 downregulated) showed a significant difference in 10 Gy irradiated lung tissues, including mucic acid, methyl-β-d-galactopyranoside, quinoline-4-carboxylic acid, and pyridoxine. There were 31 differential metabolites (16 upregulated and 15 downregulated) between 20 Gy irradiated and nonirradiated lung tissues, including taurine, piperine, 1,2,4-benzenetriol, and lactamide. The Kyoto Encyclopedia of Genes and Genomes–based pathway analysis enriched 32 metabolic pathways between the irradiated and nonirradiated lung tissues, including pyrimidine metabolism, ATP-binding cassette transporters, aminoacyl-tRNA biosynthesis, and β-alanine metabolism. Among the dysregulated metabolites, we found that taurine promoted clonogenic survival and reduced radiation-induced necrosis in human embryonic lung fibroblast (HELF) cells. This study provides evidence indicating that radiation induces metabolic alterations of the lung. These findings significantly advance our understanding of the pathophysiology of radiation-induced lung injury from the perspective of metabolism.
研究领域:
医学
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放射生物学
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代谢组学
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DOI
PubMed
高影响因子
MATERIALS LETTERS
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2012
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IF 2.307
Meiling Chen,Jintang Zhu,Guijin Qi,Changcheng He,Huiliang Wang
Biological gels are generally anisotropic in both structures and mechanical properties , but conventional synthetic hydrogels are generally isotropic. We developed a directional freezing and radiation-induced polymerization and crosslinking (DFRPC) method to fabricate anisotropic hydrogels starting from monomers. The obtained poly(2-hydroxyethyl methacrylate) (PHEMA) hydrogels show significant anisotropy in both microstructures and mechanical properties. The hydrogels exhibit an aligned porous structure along the freezing direction and an irregular porous structure in the vertical direction. Their elastic modulus and tensile stress in the parallel direction are significantly higher than those in the vertical direction, and the difference between the two directions decreases as the increase of HEMA concentration. This novel methodology offers a promising way to fabricate a wide variety of anisotropic hydrogels, and the anisotropic hydrogels may find practical applications in biomedical fields.
研究领域:
材料科学
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生物医学工程
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DOI
高影响因子
Pharmaceutics
·
2022
·Vol. 14
·期 11
·2281
·
IF 6.525
Qian Li,Yizhi Zhang,Jinglu Hu,Bochuan Yuan,Pengcheng Zhang,Yaxin Wang,Xu Jin,Lina Du,Yiguang Jin
Radiation-induced brain injury (RBI) is a common neurological disease caused by ionizing radiation (IR). Edaravone (EDA) is a free radical scavenger, has the potential to treat RBI. EDA loaded temperature-sensitive gels (TSGs) were prepared for subcutaneous injection to improve inconvenient administration of intravenous infusion. RBI mice model was established by irradiation of60Co γ-ray on head. EDA TSGs could improve spontaneous behavior, learning and memory and anxiety of RBI mice by behavior tests, including the open field test, the novel object recognition test, the elevated plus maze test and the fear conditioning test. The therapeutic effects were enhanced with the assistance of ultrasound. Alleviative pathological changes, decreased the expression of Molondialdehyde (MDA) and Interleukin-6 (IL-6) in the hippocampus of brain, indicated reduced oxidative stress and inflammatory response with the treatment of EDA TSGs and ultrasound. Moreover, ultrasound was superior to the use of EDA TSGs. Safe and effective EDA TSGs were prepared for RBI, and the feasibility of brain-targeted drug delivery enhanced by ultrasound was preliminarily demonstrated in this study.Keywords:radiation-induced brain injury;temperature-sensitive gels;edaravone;ultrasound;behavior tests
研究领域:
神经学
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药剂学
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放射生物学
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DOI
PubMed
高影响因子
Materials Today Bio
·
2025
·
IF 10.2
Mengyue Zhu,Guang Shi,Renxin Chen,Zouwei Li,Junwu Wang,Zijian Wu,Lanhong Guo,Yongchang Wei,Jingfeng Li
Radiation-induced wounds (RIWs), frequently resulting from radiotherapy, adversely affect clinical outcomes and patient care due to excessive reactive oxygen species (ROS) production, persistent inflammation, and ensuing cellular senescence. This study presents an innovative multifunctional dual-network hydrogel that integrates P1R16, a supramolecular parathyroid hormone-related peptide, into a matrix composed of quaternized chitosan (QCS), oxidized hyaluronic acid (OHA), and paramylon (PM). The hydrogel was designed to address key challenges in RIWs management, including bacterial infection, oxidative stress, cellular senescence, vascularization and tissue regeneration. Its antibacterial properties stem from QCS and PM, while the Schiff-base reaction and ether bonds enhance self-healing and mechanical stability. P1R16 contributes to reducing radiation-induced oxidative damage, inflammation, and senescence, promoting cell viability and facilitating wound healing. A rat model with RIWs demonstrated that QOP@P1R16 effectively accelerates skin regeneration via antibacterial, antioxidant, anti-senescence, and angiogenesis mechanisms. These findings suggest that QOP@P1R16 represents a promising therapeutic strategy for RIWs and other radiation-induced tissue injuries.
研究领域:
生物材料学
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再生医学
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放射生物学
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DOI
PubMed
高影响因子
BIOMEDICINE & PHARMACOTHERAPY
·
2023
·
IF 7.5
Lingxiang Ran,Qiu Chen,Xingyu Lu,Zhixiang Gao,Fengmei Cui,Xiaolong Liu,Boxin Xue
Background Male infertility is a worldwide problem but few treatments, especially irradiation-induced testicular injury. The aim of this research was to investigate novel drugs for the treatment of irradiation-induced testicular injury. Methods We administered dibucaine (0.8 mg/kg) intraperitoneally to male mice (6 mice per group) after five consecutive daily 0.5 Gy whole-body irradiation, and evaluated its ameliorating efficacy by testicular HE staining and morphological measurements. Drug affinity responsive target stability assay (Darts) were used to find target protein and pathway; mouse primary Leydig cells were isolated and to explore the mechanism (Flow cytometry, Western blot, and Seahorse palmitate oxidative stress assays); finally rescue experiments were completed by combining dibucaine with fatty acid oxidative pathway inhibitors and activators. Results The testicular HE staining and morphological measurements in dibucaine treatment group was significantly better than that in irradiation group (P < 0.05); sperm motility and mRNA levels of spermatogenic cell markers were also higher than those in the latter (P < 0.05). Darts and Western blot results showed that dibucaine targets CPT1A and downregulate fatty acid oxidation. Flow cytometry, Western blot, and Palmitate oxidative stress assays of primary Leydig cells demonstrated that dibucaine inhibits fatty acid oxidation in Leydig cells. Dibucaine combined with etomoxir/baicalin confirmed that its inhibition of fatty acid oxidation was beneficial in ameliorating irradiation-induced testicular injury. Conclusions In conclusion, our data suggest that dibucaine ameliorates irradiation-induced testicular injury in mice by inhibiting fatty acid oxidation in Leydig cells. This will provide novel ideas for the treatment of irradiation-induced testicular injury.
研究领域:
药理学
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生殖生物学
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放射生物学
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DOI
PubMed
高影响因子
Materials Today Communications
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2020
·
IF 2.678
Jingxia Wang,Chaorong Peng,Hao Chen,Weifeng Zhao,Changsheng Zhao
Polyethersulfone (PES) and its derivatives are chemical resistant, thermally stable and excellent processable, which have been widely used in filtration and hemodialysis fields. However, the hydrophobicity and bioinert of pristine PES limit the application in hemodialysis. In this study, PES derivatives were successfully prepared in homogeneous phase system via gamma irradiation polymerization of sodium styrene sulfonate (SSS), acrylic acid (AA) and N-pyrrolidone (NVP). The obtained derivatives of PES-g-SSS, PES-g-NVP, PES-g-AA/SSS, PES-g-NVP/SSS, and PES-g-AA/NVP/SSS could be directly used to prepare membranes using dimethylacetamide as solvent by phase inversion method. Then, Fourier transform infrared (FTIR) spectroscopy, X-ray photoelectron spectroscopy (XPS), scanning electron microscope (SEM), water contact angle, and surface zeta potential analyses were used to characterize the structure of modified PES membranes. The results of ultrafiltration experiment showed that the water flux increased 104.4% for PES-g-AA/SSS and 103.7% for PES-g-AA/NVP/SSS compared with the pristine PES. Moreover, the contact angle, protein adsorption and platelet adhesion decreased in all modified membranes, especially the PES-g-AA/NVP/SSS membrane has no platelet adhesion at all. The hemocompatibility of the membranes was further tested by complement activation, clotting time and hemolysis ratio, which showed the modified membranes have good hemocompatibility. The proposed one-step radiation-induced homogeneous polymerization approach opens a new route to fabricate advanced PES derivatives as blood-contacting materials.
研究领域:
材料科学
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生物医学工程
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产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2016
·
IF 4.219
Qianhong Gao,Jiangtao Hu,Rong Li,Lijuan Pang,Zhe Xing,Lu Xu,Mouhua Wang,Xiaojing Guo,Guozhong Wu
A new kind of non-fluorine-based organic-inorganic hybrid superhydrophobic cotton fabric was successfully prepared by simultaneous radiation-induced graft polymerization of γ-methacryloxypropyl trimethoxy silane (MAPS) and subsequent end-capping modification with hexamethyldisilazane (HMDS). The chemical structure and surface topography of the pristine and modified cotton fabrics were investigated in detail by ATR-FTIR, XPS, 29 Si NMR, SEM and TGA to confirm that the graft reaction and end-capping modification had taken place. The above results demonstrated that the grafting polymerization and following end-capping reaction were completed, and a grafting layer was immobilized onto the surface of the cotton fabric. Surface wettability measurement and oil-water separation showed that the modified cotton surface not only exhibited the superhydrophobicity with a water contact angle of 165°, but also afforded a high efficiency of oil-water separation (96%). In particular, this modified cotton fabric retains superhydrophobicity even after 30 laundering cycles or 400 cycles of abrasion.
研究领域:
材料科学
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纺织工程
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表面化学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Zhiwei He,Mingshuo Tang,Zhihao Wang,Yunlong Wang
Shape adaptive radiochromic dosimeters have potential in the field of radiotherapy, yet they present significant challenges. In this work, transparent and liquid radiochromic photonic crystals (RPCs) were developed by dispersing monodispersed silica particles in poly(ethylene glycol) diacrylate (PEGDA, Mn = 200). The liquid photonic crystals can adapt their shape to the required morphology in radiotherapy, and their reflectance intensity can be enhanced by the radiation-induced polymerization of PEGDA, showing a linear relationship with absorbed radiation doses. Moreover, the liquid dosimeter is capable of detecting an absorbed dose range of 50 to 250 Gy X-ray irradiation, and a spatial resolution finer than 100 μm can be achieved. Additionally, the RPCs can be stored under ambient conditions for 7 days without changing their responsive characteristics. The liquid RPCs system reported in this work exhibits excellent shape adaptability and holds promise for meeting the demands of three-dimensional dose verification, demonstrating its potential value in radiotherapy applications.
研究领域:
医学物理
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放射治疗
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材料科学
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产品关联
DOI
高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
·
2021
·
IF 5.455
Abdul Haleem,Jun Chen,Xiao-Xiao Guo,Shi-Chang Hou,Sheng-Qi Chen,Muhammad Siddiq,Wei-Dong He
Herein, for the first time, macro-porous hydrophobic cryogels of poly( iso- decyl acrylate- co -ethylene glycol dimethacrylate) P(IDA- co -EGDMA) have been successfully fabricated through γ -ray radiation-induced cryo-polymerization. This synthesis route is highly advantageous over the reported redox and UV-radiation routes for the synthesis of hydrophobic cryogels considering the high energy and the penetrating depth of γ -ray, which favours the fabrication of larger cryogels within shorter duration. The effects of radiation time, dose rate, amount of cross-linker, solvent type and solvent amount on the cryo-polymerization were studied in detail, which were based on the measurements of gel fraction and contact angle as well as the characterizations of Fourier transform infrared spectroscopy , thermal gravimetric analysis and scanning electron microscopic examination. The fabricated macro-porous cryogels were used as a sorbent for different organic solvents and oils. Using benzene as a common organic solvent, the absorption equilibrium was reached within only 60 s and the absorption capacity varied in the range of 16–20 g/g depending on the cryogel morphology and crosslinking density. As for the halogenated solvents, P(IDA- co -EGDMA) cryogels absorbed 25–29 g/g of chlorobenzene, bromobenzene, iodobenzene and tetrachloromethane. Those cryogels withstood high temperature (150 °C) deprived of any alteration in absorption capability and pore morphology. The cryogels reusability was also confirmed with keeping absorption capacity and cryogel mass up to 10 cycles.
研究领域:
材料科学
·
环境科学
·
化学工程
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）