CHEMOSPHERE
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2020
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IF 5.778
Yifei Leng,Jianguo Bao,Henglin Xiao,Dandan Song,Jiangkun Du,Sanjeeb Mohapatra,David Werner,Jun Wang
The threat of antibiotics in the environment causing antibiotics resistance is a global health concern. Enzymes catalyze pollutant transformations, and how commercially available enzymes like horseradish peroxidase (HRP), with or without a redox mediator, may be used to degrade antibiotics in water treatment is of great interest. This work demonstrates tetracycline transformation by HRP, and how it is significantly enhanced by free radicals created from the mediator 2,2-Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). Water temperature and pH strongly influence the tetracycline removal rate due to their correlation with the enzyme activity , abundance and stability of ABTS •+ . Four transformation products were identified in the pure HRP system using a liquid chromatography tandem mass spectrometry hybrid quadrupole-orbitrap mass spectrometer system. Addition of 25 μmol L −1 ABTS not only accelerated the degradation of tetracycline, but also expanded the range of degradation pathways. Potential tetracycline transformation pathways are proposed based on these observations, which include a range of mechanisms such as hydroxylation, demethylation, dehydration, decarbonylation and secondary alcohol oxidation. Despite of decreased efficiency, the HRP/ABTS system was able to degrade tetracycline in a domestic wastewater treatment plant effluent matrix, which demonstrates the potential of the system to be utilized in wastewater treatment.
研究领域:
环境科学
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生物化学
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DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
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2020
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IF 5.065
Wei Jin,Rongrong Wang,Xirong Huang
The horseradish peroxidase (HRP) mediated synthesis of conducting polyaniline (PANI) was investigated in detail in the bicontinuous microemulsion stabilized by both sodium bis(2-ethylhexyl) sulfosuccinate (AOT) and dodecyl sulfobetaine (SB-12). The addition of zwitterionic surfactant SB-12 not only reduces the phase inversion temperature of the anionic surfactant AOT stabilized microemulsion, but also enhances the catalytic performance of the solubilized HRP. Compared with the previously reported AOT/SDS stabilized bicontinuous microemulsion, the present AOT/SB-12 stabilized bicontinuous microemulsion is much more suitable for the expression of the catalytic activity of HRP; moreover, the conductivity of the resulting PANI improves greatly (a 15-fold increase in conductivity). The present results indicate further that the catalytic activity of HRP and its stability are crucial for the biosynthesis of PANI in bicontinuous microemulsions. To understand the underlying mechanism of the medium effects, electron spin resonance (ESR), UV–vis-NIR and small-angle X-ray scattering (SAXS) spectroscopies were used to investigate the effects of the oil-to-water ratio ( α ) and the surfactant concentration ( γ ) of the present bicontinuous microemulsion on the conductivity of resulting PANI. Results show that the bicontinuous microemulsion stabilized by both AOT and SB-12 still has a template role in the biosynthesis of PANI and the PANI conductivity is mainly determined by the microstructure of the template and by the microstructure-dependent activity and stability of the solubilized HRP. Lower α and γ values are beneficial to the enzymatic synthesis of high-quality PANI. The present study is of great help not only to deepen the understanding of the template role of anionic surfactant aggregates in the biosynthesis of conducting PANI, but also to develop new strategies for the biosynthesis of high quality PANI.
研究领域:
生物化学
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材料科学
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DOI
高影响因子
Journal of Analytical Science and Technology
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2021
·Vol. 12
·期 1
·1-9
·
IF 2.769
Dong Bin,Fan Qian,Li Ming,Huan Yanfu,Feng Guodong,Shan Hongyan,Fei Qiang
In this study, N-(4-aminobutyl)-N-ethylisoluminol (ABEI) was used as an energy donor, while sodium fluorescein was used as an enhancer and energy acceptor, which resulted in it producing resonance energy transfer and greatly increasing the strength of chemiluminiscence (CL). When horseradish peroxidase (HRP) is added, hydrogen peroxide (H2O2) will quickly separate into hydroxyl radicals (·OH) and superoxide ions (O2·−). If tyrosine (Tyr) is present in the system, the hydroxyl group on the benzene ring of Tyr robs ·OH and O2·− in the CL system, thereby reducing the intensity of CL. Based on this phenomenon, a luminescence system of ABEI and sodium fluorescein system was established to detect Tyr for the first time. This method has an ultra-low detection limit and a wide linear range, and is cheap and easy to operate. Under various optimal conditions, the linear range is from 3.0×10−8 to 3.0×10−5 mol/L, and the limit of detection is 2.4×10−8 mol/L. It has been successfully used in the detection of dairy products with satisfactory results.
研究领域:
生物化学
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分析化学
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DOI
高影响因子
Nanoscale
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2025
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IF 5.1
Zuyao Fu,Lingfeng Yang,Zhaoyang Ding,Jing Xie
Immobilized enzymes play a crucial role in analytical sensing due to their exceptional stability and considerable commercial importance. In this study, a stable Zr-based metal–organic framework (UiO-66-NH2) was prepared as an immobilization platform for horseradish peroxidase (HRP) through covalent binding. HRP@UiO-66-NH2 retained 75% of its activity after 10 cycles. Subsequently, a colorimetric/fluorometric dual-mode sensing strategy using HRP@UiO-66-NH2 was developed for nitrite detection. HRP@UiO-66-NH2 facilitated the transformation of the non-colored compound 3,3′,5,5′-tetramethylbenzidine (TMB) into its blue oxidized form. When nitrite is added, oxidized TMB (ox-TMB) specifically engaged with nitrite (NO2−) to generate diazotized TMB, leading to a color shift from blue to yellow. Concurrently, NO2− reacted with the amino groups of HRP@UiO-66-NH2, forming diazonium compounds and suppressing the fluorescence of HRP@UiO-66-NH2. The limits of detection were 0.21 μM and 0.19 μM for the colorimetric and fluorometric strategies, respectively. Furthermore, a portable kit for detecting nitrite was created by integrating gelatin with HRP@UiO-66-NH2. The kit could visually identify nitrite from 10–400 μM in colorimetric mode and 0–400 μM in fluorometric mode. This method provides an innovative approach for nitrite sensing, paving the way for new research into multifunctional immobilized enzymes and their potential uses in biochemical sensing applications.
研究领域:
生物化学
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分析化学
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DOI
PubMed
高影响因子
FIBERS AND POLYMERS
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2021
·Vol. 22
·期 8
·2145-2155
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IF 2.153
Baek Na-won,Fan Xue-rong,Yuan Jiu-gang,Xu Jin,Wang Qiang
Due to the increasing importance of environmentally friendly processes, the development of monomer synthesis and its polymerization using enzymes has been investigated throughout the world. In this paper, the polymerization mechanism between silk fabrics and gallic acid (GA) in the presence of horseradish peroxidase (HRP) has been studied. The polymerization mechanism was studied in detail using UV-Vis and Fourier transform infrared spectroscopy (FT-IR), and high-performance liquid chromatography-electrospray ionization mass spectrometry (HPLC-ESI-MS) analysis. In addition, the dyeing properties and optimum reaction conditions were further investigated using the K/S values. The color fastness of the resulting silk fabrics has also been discussed. The results show that the dyeing mechanism for GA and silk fabric involved linking via the -NH 2 and -OH groups in silk fiber and the -OH groups in GA. This was probably formed via the formation of phenyl-phenyl, oxygen-phenyl, or amino-phenyl linkages. HRP significantly improved the dyeing properties of GA dyes toward silk fabrics. The optimum reaction conditions for dyeing silk fabrics have been proposed (Incubation period, 5 h; temperature, 50 °C; GA dosage, 10 mM; HRP, 6 U/ml, and H 2 O 2 , 5 mM). The color fastness of the resulting silk fabrics displayed high colorfastness in all the colorfastness tests performed.
研究领域:
纺织科学
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生物化学
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DOI
高影响因子
ELECTROCHIMICA ACTA
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2022
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IF 7.336
Lu Yang,Nan Wu,Rongrong Bai,Minjiao Chen,Weiliang Dong,Jie Zhou,Min Jiang
We report a facile strategy for the detection of pyruvate in fermentation progress based on a non-enzymatic decarboxylation reaction of pyruvate with hydrogen peroxide (H 2 O 2 ), coupled with a horseradish peroxidase (HRP)-incorporating nanomaterial as an enzyme element to replace conventional pyruvate oxidase (POX). HRP-Cu 3 (PO 4 ) 2 •3H 2 O nanoflowers (HRP-CuP NFs) and thiol graphene (tG) were immobilized onto an Au disk electrode via layer-by-layer assembly to construct an improved amperometric H 2 O 2 biosensor. The as-prepared HRP-CuP NF/tG/Au electrode exhibited a high electrocatalytic performance toward H 2 O 2 , showing a sensitive response of 67.6 μA mM -1 cm -2 , as well as a linear range from 0.1-8.2 mM with a detection limit (LOD) of 0.06 mM (S/N=3). Furthermore, the resulting biosensor also demonstrated a linear range for pyruvate detection from 1.0 to 8.0 mM with LOD of 0.3 mM (S/N=3), under a working potential of -0.3 V. Finally, the biosensor was successfully used for the determination of pyruvate in real microbial fermentation processes without pretreatment, showing an excellent correlation coefficient compared to the standard HPLC assay with RSD values of 3.03-6.45%. This work provides a new approach for the design and preparation of portable sensors for indirect pyruvate detection with high performance, low cost, and easy operation.
研究领域:
生物化学
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传感器技术
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发酵工程
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DOI
高影响因子
POLYMER JOURNAL
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2024
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IF 2.3
Wang Wenda,Wang Qiang,Wang Ping
Acrylic acid (AA)/methyl acrylate (MA)-modified starch was prepared via a catalytic process involving horseradish peroxidase (HRP)/acetylacetone (ACAC) as an initiation system. The structural analysis results demonstrated that the grafting of AA and MA onto starch was successful. Furthermore, the impact of the grafting reaction conditions on the grafting effect and the characteristics of the resulting grafted products were also examined. The results demonstrated that AA and MA were successfully grafted onto starch. The optimal conditions for starch grafting with AA/MA were identified as a reaction time of 4 h, an HRP enzyme concentration of 12 U/mL, a total monomer/starch ratio of 4:6, and a molar ratio of AA/MA feeding of 3:7. The viscosity and viscoelasticity of the modified starch slurry increased, and the film-forming property was enhanced. Compared with the original starch film, the modified film markedly increased the flexibility and tensile strength. Furthermore, an increased acrylic acid content maintained the original hydrophilicity of the modified starch film.
研究领域:
材料科学
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生物催化
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DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
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2022
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IF 10.753
Yifei Leng,Feiyu Liu,Huiping Cai,Fengyi Chang,Wen Xiong,Shushi Huang,Jun Wang
The catalysis of HRP coupling with redox mediator was a feasible technology for the transformation of antibiotics. This work screened three effective redox mediators syringaldehyde (SYR), acetosyringone (AS) and p -coumaric acid (PCA) for the norfloxacin (NOR) transformation in HRP/redox mediator system. Then, compared their transformation characteristics under varying conditions. The molecular docking results indicated HRP catalytic mediator was spontaneous, and the absolute value order of free energy between three redox mediators and HRP was consistent with the order of NOR removal in experiment. The presence of humic acid (HA) and polystyrene (PS) microplastics could block the removal of NOR, and the inhibition effect was proportional to the level of HA and PS particles. Seven and six possible intermediate products were identified by using SYR/AS and PCA as redox mediators, respectively, and potential NOR transformation pathways were proposed. SYR and AS treatment had the same transformation products and pathways due to their similar structure, including defluorination , oxidation, cross-coupled with mediator, demethylation and dehydrogenation . While for the PCA group, NOR not only performed the above action (except defluorination), but also underwent decarbonylation. These findings may expand our knowledge of the conversion and fate of fluoroquinolones through HRP coupled with redox mediator in the environment.
研究领域:
环境科学
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生物学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2022
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IF 9.231
Yiting Guo,Bengang Wu,Xiuyu Guo,Dandan Liu,Chengcheng Qiu,Haile Ma
This study aimed to investigate the effect of thermosonicaion (TS) with different frequency modes on the horseradish peroxidase (HRP) inactivation from the aspects of ultrasound field and enzyme structural changes. The relationships between the activity and structural modification were also evaluated. A thermal-only treatment was performed to estimate the action of ultrasound in TS treatment. Compared to mono-frequency, especially the 33 kHz, TS with dual- and tri-frequency modes showed an intensive inactivation impact on HRP. Exogenous hydroxyl radical test showed the hydroxyl radical played a vital role in HRP inactivation. Changes in hematin group, tertiary and secondary structures were also confirmed, and the inactivation process followed an all-or-none model. Morphological images indicated the aggregation and deformation of HRP molecules during TS treatment, and roughness values were changed accordingly. Pearson correlation analysis implied that the structural destruction on the spatial conformation of HRP was strongly related to its activity.
研究领域:
生物化学
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食品科学
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产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2023
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IF 8.6
Jiacong Wu,Xinnan Ma,Ting He,Juan Han,Yan Zhu,Chunmei Li,Yun Wang
The structural limitation of the photocatalyst itself is the main reason for the difficulty in increasing the enzyme loading amount in the photo-enzyme coupling system. Hereon, a g-C 3 N 4 -based photocatalyst ACN 45 with high enzyme loading amount was synthesized. Abundant 5 nm channels match well with the size of horseradish peroxidase (HRP) molecules, which enables the loading amount to reach 6.3 times that of pure g-C 3 N 4 . Meanwhile, the microenvironment provided by channels of ACN 45 significantly improves the activity and stability of the immobilized enzyme, and reduces the leaching of the enzyme molecules inside it. The internal immobilization of HRP did not affect the absorption of visible light by the photocatalyst, and high loading amount of photo-enzyme coupling system enhance the photocatalytic degradation of BPA. The degradation rate reached 94 % within 1 h, which is 5.9 times that of pure g-C 3 N 4 . The research findings open avenues for designing a photo-enzyme coupling system with high enzyme loading and high stability through simple steps.
研究领域:
环境科学
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生物化学
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产品关联
DOI
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
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2020
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IF 3.448
Chuankai Yang,Miaorong Zhang,Wei Wang,Yao Wang,Jianguo Tang
A colorimetric platform based on enzyme-inorganic hybrid nanoflowers was used for ultraviolet-visible detection of hydrogen peroxide (H 2 O 2 ). The hybrid nanoflowers were prepared via incubation at room temperature using horseradish peroxidase (HRP) and copper phosphate (Cu 3 (PO 4 ) 2 ) as the organic and inorganic components, respectively. HRP/Cu 3 (PO 4 ) 2 hybrid nanoflowers (HRP-HNFs) showed linear H 2 O 2 detection range from 100 nM to 100 μM, moreover, HRP-HNFs exhibited good selectivity for H 2 O 2 detection due to the specificity of HRP. In addition, HRP-HNFs have good reusability, excellent color stability and long-term storage stability, which indicate HRP-HNFs are promising for catalysis and biomedical applications.
研究领域:
生物化学
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分析化学
·
纳米材料科学
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DOI
高影响因子
WATER SCIENCE AND TECHNOLOGY
·
2023
·Vol. 88
·期 6
·1428-1437
·
IF 2.7
Li Haitao,Guo Linfeng,Li Yingying,Chen Min,Bai Chunlu,Song Aolei,Cheng Linxiu,Chen Xueli,Chen Yonglin
Bisphenol A (BPA) is one of the most widely used chemical products, which is discharged into rivers and oceans, posing great hazards to organisms such as reproductive toxicity, hormone imbalance and cardiopathy induction. With the expansion harm of BPA, people have paid more attention to the environmental effects. In this paper, the degradation of BPA from the synthetic wastewater using the immobilization of horseradish peroxidase membrane reactor (HPR) was investigated. The immobilized HRP microporous membrane was prepared by the porous calcium alginate method. In addition, the reuse of the immobilized HPR membrane and the measurement of membrane flux showed that the membrane has good activity and stability. Finally, the experimental parameters including reaction time, pH, the concentration of BPA and the dosage of H 2 O 2 were optimized to remove the BPA, and about 78% degradation efficiency of BPA was achieved at the optimal condition as follows: H 2 O 2 to BPA molar ratio of 1.50 with an initial BPA concentration of 0.1 mol/L, the HPR dosage of 3.84 u/mL, the initial solution pH of 7.0, a temperature of 20 °C and a contact time of 10 min. Horseradish peroxidase (HRP) can be effectively immobilized on the membrane. The immobilized HRP can maintain high activity and be circulation utilization. The optimum conditions for the mole rate of H 2 O 2 /BPA dosage and the pH value are 1.5 and 7, respectively.
研究领域:
环境科学
·
生物化学
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DOI
高影响因子
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