JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH
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2021
·
IF 1.571
Gen-Qiang Chen,Di Sun,Jin-Ming Yang,Song Zhang,Yue-E Tian,Zhi-Ping Che,Sheng-Ming Liu,Jia Jiang,Xiao-Min Lin
Two series of sulfonate derivatives of carvacrol and thymol were synthesized and screened in vitro for their anti-oomycete activity against Phytophthora capsici, respectively. Among all of 32 derivatives, five compounds 3a, 4a, 4k, 3n, and 4n exhibited more potent anti-oomycete activity against P. capsici with EC50 values of 66.66, 62.94, 68.65, 61.24, and 52.91 mg/L, respectively. This suggested that introduction of different substitutions at the hydroxyl position of 1/2 could have remarkable effect on anti-oomycete activity. Overall, when R1 = isopropyl and R2 = methyl, the anti-oomycete activities of the compounds were higher than that of the corresponding compounds of R1 = methyl and R2 = isopropyl.
研究领域:
植物化学
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农药科学
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微生物学
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产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2025
·
IF 3.8
Wenhao Liang,Xiaoxiao Xu,Tong Shi,Shengyang Xu,Qizhi Wang,Bi Wang
BACKGROUND Phytophthora capsici is a devastating oomycete pathogen with major economic concern. However, the use of traditional chemical fungicides is associated with environmental pollution, and fungicide resistance is increasingly prevalent. Consequently, developing safe, effective, and environmentally friendly alternatives is critically required. Evodiamine (EVO), a bioactive alkaloid from plants, has been extensively investigated for its anti-cancer properties. However, its inhibitory effects against phytopathogens have not yet been fully elucidated. This study evaluated EVO's antimicrobial activity toward P. capsici and its potential mode of action. RESULTS EVO significantly inhibited P. capsici mycelial growth and spore germination, with median effective concentration (EC 50 ) values of 6.48 μg mL −1 and 7.13 μg mL −1 respectively, across 108 P. capsici isolates. EVO exerted its antimicrobial effects by disrupting the cellular integrity of P. capsici , specifically targeting both the cell membrane and wall. Furthermore, EVO exhibited strong protective and curative efficacies in pepper leaves and seedlings. Genome-wide transcriptional analysis revealed that EVO treatment significantly altered the gene expression profile of P. capsici , especially affecting genes relevant to cell membrane integrity and transporter activity. CONCLUSION Overall, this study revealed that EVO showed great control efficacy against P. capsici and held promise as a novel botanical fungicide. © 2025 Society of Chemical Industry.
研究领域:
植物病理学
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农业微生物学
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分子植物-病原互作
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天然产物药理学
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农药学
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产品关联
DOI
PubMed
高影响因子
MOLECULES
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2023
·Vol. 28
·期 7
·2958
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IF 4.6
Bi Wang,Guodong Zhang,Jingjing Yang,Linwei Li,Pirui Li,Shu Xu,Xu Feng,Yu Chen
Phytophthora capsiciis a highly destructive phytopathogenic oomycete with a broad host range and is responsible for tremendous losses. Euphorbia factor L3(EFL3) is a natural plant-derived compound that has been widely studied in medicine and cosmetic applications. In this study, the sensitivity of 105P. capsiciisolates to EFL3was determined, and the biological activity and physiological effects of EFL3againstP. capsiciwere investigated. The median effective concentration (EC50) values for EFL3inhibition mycelial growth and spore germination ranged from 0.66 to 8.94 μg/mL (mean, 2.96 ± 0.91 μg/mL) and 1.63 to 13.16 μg/mL (mean, 5.30 ± 1.64 μg/mL), respectively. EFL3treatment resulted in cell wall and cell membrane damage ofP. capsici, which was revealed by morphological and ultrastructural observations, propidium iodide (PI) and calcofluor white (CFW) staining, and measurements of relative conductivity as well as malondialdehyde (MDA) and glycerol contents. In addition, the contents of phospholipid and cellulose, which are the major components of cell membrane and cell wall, were significantly reduced following EFL3treatment. Furthermore, EFL3provided protective as well as curative efficacies againstP. capsicion detached tomato leaves and pepper seedlings in vivo. These data show that EFL3exhibits strong inhibitory activity againstP. capsici, thereby suggesting that it could be an effective alternative for controllingP. capsici-induced diseases.Keywords:baseline sensitivity;botanical fungicide;Euphorbia factor L3;action mechanism;Phytophthora capsici
研究领域:
植物病理学
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药理学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2022
·Vol. 78
·期 6
·2240-2249
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IF 4.462
Tan Dai,Zhiwen Wang,Xingkai Cheng,Huige Gao,Li Liang,Pengfei Liu,Xili Liu
BACKGROUND Fungicide resistance has become a serious problem for different mode of action groups except for uncouplers, which makes their resistance mechanism a hot topic, which until now, has not been well clarified. SYP-14288, a newly developed diarylamine fungicide modeled on fluazinam, has shown good toxicity to both oomycete and fungus by the action of uncoupling. In this research, the resistance of Phytophthora capsici to SYP-14288 was studied to clarify the resistance mechanism of uncouplers. RESULTS The toxicity tests of resistant strains against SYP-14288 showed multidrug resistance. The high-performance liquid chromatography (HPLC) results showed that resistant strains could efflux the fungicide, and this ability could be inhibited by the efflux pump inhibitor amitriptyline. The target protein of amitriptyline is P-glycoprotein (P-gp), which was overexpressed in resistant strains. Three products of nitrate reduction of SYP-14288 were detected and determined by HPLC-Q-TOF. Eight cytochrome P450 monooxygenase (P450) proteins were differentially involved in the reduction reaction. CONCLUSION Both fungicide efflux and detoxification metabolism were involved in the resistance mechanisms of P. capsici to SYP-14288. © 2022 Society of Chemical Industry.
研究领域:
植物病理学
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分子生物学
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杀菌剂科学
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DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2018
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IF 3.44
Bi Wang,Shu Xu,Yan Cao,Fei Liu,Xingzeng Zhao,Xu Feng
10-deacetyl-bacatin III (10-DAB) is a natural plant-derived taxane diterpene, whose antimicrobial activity against phytopathogens remains unknown. In this study, we demonstrated the antimicrobial effect of 10-DAB on plant-pathogenic oomycetes . Our results revealed that 10-DAB exhibited significant antimicrobial activities against test oomycetes, especially against Phytophthora capsici , with a median effective concentration (EC 50 ) of 1.46 μg/mL, but had no effect on test fungi. Under 10-DAB treatment, mycelia of P. capsici were contorted with an increased number of top branches, and the production and germination of zoospores were inhibited and delayed, respectively. In addition, 10-DAB had favorable protective and curative activities with control efficacies of 63.90% and 74.81% at 200 μg/mL on detached pepper leaves. Furthermore, 10-DAB caused a significant decrease in soluble protein , lysine, and α, ε-diaminopimelic acid content of P. capsici , which suggested that 10-DAB inhibited the lysine biosynthesis . On the contrary, treatment with exogenous lysine effectively counteracted 10-DAB's inhibition activity on P. capsici . Moreover, relative expression of four key lysine biosynthesis-related genes of P. capsici were decreased upon 10-DAB treatment. Taken together, our findings suggest a lysine biosynthesis inhibiting-dependent antimicrobial activity of 10-DAB against P. capsici , which contributes to accelerating the application of 10-DAB for successful management of phytophthora blight disease in agricultural production.
研究领域:
植物病理学
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分子生物学
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农业科学
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产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2023
·
IF 4.1
Bi Wang,Jingjing Yang,Xingzeng Zhao,Xu Feng,Shu Xu,Pirui Li,Linwei Li,Yu Chen
BACKGROUND Phytophthora capsici is an extremely destructive phytopathogenic oomycete that causes huge economic losses. However, due to the drug resistance risk and environmental threat of chemical fungicides, it is necessary to develop environmentally friendly biocontrol alternatives. Rhein is a major medicinal ingredient of traditional Chinese herbs, and it is widely used in the medical field. However, its inhibitory effect against phytopathogens is unknown. Herein, the antifungal spectrum of rhein and its possible action mechanism against P. capsici were investigated. RESULTS Rhein possessed broad-spectrum antifungal activity against phytopathogens, particularly P. capsici , Phytophthora infestans , Helminthosporium maydis , and Rhizoctonia solani . Rhein inhibited the mycelial growth as well as the spore germination of P. capsici with mean 50% effective concentration (EC 50 ) values of 4.68 μg mL −1 and 6.57 μg mL −1 against 117 P. capsici isolates, respectively. Rhein effectively suppressed the occurrence and spread of Phytophthora blight and significantly destroyed the cell membrane permeability and integrity of P. capsici , corroded its cell wall integrity, and damaged its morphology and ultrastructure. Moreover, rhein caused a considerable reduction in the phospholipid and cellulose contents. Genome-wide transcriptional profiling of P. capsici in response to rhein indicated significant reduction in the expression levels of genes participating in glycerolipid metabolism and starch and sucrose metabolism. Additionally, rhein strengthened the disease defense system of pepper by enhancing related enzyme activities. CONCLUSION This study demonstrated that rhein could effectively inhibit P. capsici using multiple mechanisms of action. Rhein has the potential to be an efficient alternative to control diseases caused by P. capsici . © 2023 Society of Chemical Industry.
研究领域:
植物病理学
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分子生物学
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药理学
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农业科学
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产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2024
·
IF 3.8
Xiaoqun Yang,Shichun Jiang,Meng Zhang,Tingting Li,Zhichao Jin,Xingxing Wu,Yonggui Robin Chi
BACKGROUND Plant fungal diseases pose a significant threat to crop production. The extensive use of chemical pesticides has led to growing environmental safety risks and pesticide resistance of various plant pathogens. Therefore, it is an urgent task to explore novel eco-friendly fungicidal agents with high efficacy to combat fungal infection. RESULTS In this study, we rationally designed a series of novel thymol derivatives by incorporation of the sulfonamide moiety and evaluated their biological activities against plant pathogenic fungi. The bioassay results underscored the remarkable in vitro antifungal activity of compounds 5m and 5t against Phytophthora capsici ( P. capsici ), with EC 50 values of 8.420 and 8.414 μ g/mL, respectively. Their efficacies were superior to that of widely used commercial fungicides azoxystrobin (AZO, 20.649 μ g/mL) and cabendazim (CAB, 251.625 μ g/mL). Furthermore, compound 5v exhibited excellent in vitro antifungal activity against Sclerotinia sclerotiorum ( S. sclerotiorum ), with an EC 50 value of 12.829 μ g/mL, significantly outperforming AZO (63.629 μ g/mL). In vivo bioassays demonstrated the impactful activity of compound 5v against S. sclerotiorum , achieving over 98% curative and protective efficacies at the concentration of 200 μ g/mL. Further mechanistic investigations unveiled that compound 5v induced mycelial shrinkage and collapse in S. sclerotiorum , resulting in organelle damage and the accumulation of antioxidant enzyme activity. CONCLUSION The significant antifungal efficacy of the prepared thymol derivatives shall encourage further exploration of compound 5v as a promising candidate to develop novel fungicides for crop protection. © 2024 Society of Chemical Industry.
研究领域:
农业科学
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植物病理学
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化学生物学
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Tianbing Zhou,Yuxin Xu,Yingtong Chen,Zhengbin Tang,Erxin Wang,Mengmeng Li,Mingrui Dou,Zhijia Zhang,Tao Zhang,Shujun Huang,Shuxian Gu,Shuai Wang,Ranfeng Sun,Dong Li
Phytophthora capsici causes devastating losses in global pepper production. This study deciphers a sophisticated plant-microbe-metabolite collaboration initiated by rhizosphere-colonizing Streptomyces. We demonstrate that Streptomyces specifically primes pepper root biosynthesis of 6-nitrocoumarin, a nitrated coumarin derivative exhibiting potent broad-spectrum antioomycete activity (EC50: P. capsici 44.39 mg/L, Phytophthora palmivora 47.06 mg/L, Peronophythora litchii 34.92 mg/L). Crucially, this Streptomyces-recruited metabolite functions as a keystone rhizosphere signal, selectively enriching beneficial bacterial consortia while directly disrupting the ABC transporters of pathogens. The restructured microbiome synergistically amplifies plant systemic resistance through upregulating defense genes and enhancing root immunity. Our work establishes 6-nitrocoumarin as a dual-functional inoculant, a direct actinomycete compound and microbiome modulator, revealing a novel plant-microbe codefense paradigm driven by Streptomyces-induced metabolic rewiring for sustainable crop protection.
研究领域:
植物病理学
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微生物生态学
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分子植物-微生物互作
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农业生物技术
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天然产物化学
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作物保护
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根际生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Agricultural Science & Technology
·
2022
·
IF 0
Yanke Hao,Hongfu He,Pan Zhou,Kaikai Niu,Hongjian Song,Yuxiu Liu,Jingjing Zhang,Deyu Hu,Qingmin Wang,Baoan Song
Aldisine alkaloid has a wide range of biological activities and is also the core skeleton of a variety of natural products, but its poor solubility affects its efficacy. In order to improve its solubility and biological activity, a series of indoloazepinone derivatives containing oxime ether, oxime ester, hydrazone, and ester moieties were designed and synthesized, and their antiviral, fungicidal, and larvicidal activities were evaluated. The solubility of these indoloazepinone derivatives improved in comparison with that of aldisine, and most of these compounds showed good antiviral activities against pepper mild mottle virus (PMMoV), among which the 4-chlorobenzoyl oxime ester derivative 5i showed the highest protection, curative, and inactivation activities in vivo (inhibition rate 61 ± 4.1, 60 ± 5.3, and 74 ± 4.2% at 500 mg/L, respectively), which was comparable to that of ningnanmycin (59 ± 2.2, 58 ± 0.7, and 81 ± 4.8% at 500 mg/L, respectively). Compound 5i (the rat acute oral toxicity test, LD50 > 2000 mg/kg) emerged as a promising candidate for anti-PMMoV drug. Most of these compounds have broad-spectrum fungicidal activities, and 5a and 5f showed selectively fungicidal activities against Phytophthora capsici. In addition, these compounds also showed good larvicidal activities against Plutella xylostella and Culex pipiens pallens.
研究领域:
药理学
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化学
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植物病理学
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昆虫学
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病毒学
✓ 已验证
DOI
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2022
·
IF 5.895
Biao Wang,Bo He,Tianyu Chen,Hao Li,Liyifan Chen,Yiliang Chen,Kailin Tian,Kun Yang,Danyu Shen,Wei Yan,Yonghao Ye
The fermentation of endophytic Nigrospora chinensis GGY-3 resulted in the isolation of tropolone stipitaldehyde (1), which exhibited broad-spectrum inhibition activity against fungi and bacteria, especially against Phytophthora capsici, with an EC50 value of 0.83 μg/mL and Xanthomonas oryzae pv. oryzicola, with a minimum inhibitory concentration value of 4.0 μg/mL. The in vitro and in vivo assays demonstrated that 1 had a significant protective effect on P. capsici. Furthermore, 1 inhibited the spore germination of P. capsici and damaged the plasma membrane structure. As observed by SEM and TEM, after exposure to 1, mycelia exhibited swelling, shrunken, branch-increasing phenomena, cell wall and membrane damage, and disordered content. Transcriptome analysis revealed that 1 might affect starch and sucrose metabolism and fatty acid biosynthesis by suppressing the expression of genes relevant to cell wall synthetases and cell membrane-associated genes. These findings indicate that 1 may be a potential agrochemical fungicide for controlling phytophthora blight.
研究领域:
植物病理学
·
微生物学
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分子生物学
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农业科学
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2013
·
IF 2.468
Fujia Chen,Xiaohua Long,Mengni Yu,Zhaopu Liu,Ling Liu,Hongbo Shao
Spoilage of industrial fruits and vegetables in storage and transportation due to fungal infection results in significant losses, and new natural antifungal treatments would have a large economic value. The extracts of antifungal compounds and phenolic acids from Jerusalem artichoke ( Helianthus tuberosus L.) leaves were investigated for potential use in enhancing preservation of fruits and vegetables in storage. Either crude leaf extract or n -butanol fraction was active against Botrytis cinerea, Colletotrichum gloeosporioides, Phytophthora capsici Leonian and Rhizoctonia cerealis , with the values of IC 50 (half of the maximum inhibitory concentration) ranging from 2.166 to 2.534 g L −1 for the crude leaf extract and 0.232–1.911 g L −1 for n -butanol fraction. The severity of grey mould caused by B. cinerea was significantly reduced by n -butanol fraction applied at 1 and 2 g L −1 (the control efficiency of 71.3% and 77.8%, respectively, compared with commercial preparation Carbendazim . Following in vitro activity-guided fractionation by bioautography, six phenolic acids were separated from n -butanol fraction. Among them, caffeic acid, 3,4-dicaffeoylquinic acid and 1,5-dicaffeoylquinic acid played a dominant role and were active in bioassays against Gibberella zeae , with respective minimum inhibitory concentrations (MIC) being 108, 60 and 4.2 μg mL −1 . These results imply that Jerusalem artichoke leaves might be a potential source of natural fungicides.
研究领域:
植物科学
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食品科学
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药理学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）