FOOD AND AGRICULTURAL IMMUNOLOGY
·
2018
·
IF 2.568
Jiajia Sun,Liqiang Liu,Shanshan Song,Gang Cui,Hua Kuang
The problem of waste oil is a food safety issue that requires urgent resolution. In this study, a novel hapten was designed for the rapid detection of capsaicinoids, a marker of waste oil, and a monoclonal antibody (MAb) 5D7 was generated that is specific for three major capsaicinoids: capsaicin (CPC), dihydrocapsaicin (DCPC), and N-vanillylnonanamide (N-V). The half maximal inhibitory concentrations of MAb 5D7 for CPC, DCPC, and N-V were 1.12, 0.8, and 0.87 ng/mL, respectively. An immunochromatographic strip test based on MAb 5D7 was established for these three capsaicinoids, with visual inspection limits of <1.0 ng/mL, which satisfies the requirement for the rapid on-site inspection of waste oil.
研究领域:
食品安全
·
免疫学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Li Zhong,Rui Wang,Qin-Hui Wen,Jian Li,Jia-Wei Lin,Xin-An Zeng
In this study, the binding mechanism between bovine serum albumin (BSA) and three gingerols ([6]-, [8]- and [10]-gingerol) was evaluated to explore an effective strategy for improving solubility and stability of gingerols. The fluorescence analysis suggested gingerols could bind with BSA to form a stable BSA/gingerols complex and [10]-gingerol had the strongest binding affinity ( K a = 4.016 × 10 4 L/mol) at 298 K. Thermodynamic parameters and molecular modeling validated that hydrophobic interaction and hydrogen bonds were the main driving force for the interaction of BSA/gingerols. Gingerols bound to BSA at site I (subdomain IIA) resulted in a conformational change of BSA with a structure shrinkage, which was responsible for the decrease of surface hydrophobicity. The formation of BSA/gingerols complexes promoted the solubility of [6]-, [8]- and [10]-gingerol increasing by 1.50, 6.04 and 23.50 times, respectively. In addition, the stability and antioxidant capacity of gingerols was significantly improved after binding with BSA.
研究领域:
生物化学
·
食品科学
·
药物化学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Cunlian Ji,Hongyan Yu,Zhibin Pang,Mengyao Guo,Liangmin Yu
Accurate dye and inorganic salt separation from wastewater is crucial to effective dye wastewater recovery. Loose nanofiltration membranes (LMNs) exhibiting high dye rejection and low salt retention are expected to be applied in dye/salt separation. However, membrane fouling still restricts the effective application of LMNs. In this work, a novel capsaicin-containing functional structural derivative, N-(6-methacrylamido-3,4 hydroxybenzyl)acrylamide (DHMAM), was used as an aqueous phase reactive monomer to prepare high-performance antifouling loose nanofiltration membranes (PSf-DHMAM LMNs) by interfacial polymerization with 1,3,5- benzenetricarbonyl trichloride (TMC). The obtained PSf-DHMAM LMNs have good hydrophilic and charge-negative properties. The optimized PSf-DHMAM 0.5 LMNs exhibited high permeability performance (61.24 ± 3.29 L m -2 h -1 bar -1 ), excellent dye rejection (99.46 ± 0.08% for Congo Red) and ultra-low salt retention (2.49 ± 0.1% for NaCl). In addition, PSf-DHMAM 0.5 LMNs have good antibacterial properties (the antibacterial rate against Escherichia coli can reach 89.53%), antifouling properties and long-term stability, which are promising for future applications in the field of dye/salt separation.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Dingding Li,Beibei Chu,Bo Li,Xiong Wang,Xingguang Chen,Qianhui Gu
Because of its peculiar flavor, chili oil is widely used in all kinds of food and is welcomed by people. Chili pepper is an important raw material affecting its quality, and commercial chili oil needs to meet various production needs, so it needs to be made with different chili peppers. However, the current compounding method mainly relies on the experience of professionals and lacks the basis of objective numerical analysis. In this study, the chroma and capsaicinoids of different chili oils were analyzed, and then the volatile components were determined by gas chromatography-mass spectrometry (GC–MS) and gas chromatography-ion migration spectrometer (GC-IMS) and electronic nose (E-nose). The results showed that Zidantou chili oil had the highest L*, b*, and color intensity (ΔE) (52.76 ± 0.52, 88.72 ± 0.89, and 118.84 ± 1.14), but the color was tended to be greenyellow. Xinyidai chili oil had the highest a* (65.04 ± 0.2). But its b* and L* were relatively low (76.17 ± 0.29 and 45.41 ± 0.16), and the oil was dark red. For capsaicinoids, Xiaomila chili oil had the highest content of capsaicinoids was 2.68 ± 0.07 g/kg, Tianjiao chili oil had the lowest content of capsaicinoids was 0.0044 ± 0.0044 g/kg. Besides, 96 and 54 volatile flavor substances were identified by GC–MS and GC-IMS respectively. And the main volatile flavor substances of chili oil were aldehydes, alcohols, ketones, and esters. A total of 11 key flavor compounds were screened by the relative odor activity value (ROAV). Moguijiao chili oil and Zidantou chili oil had a prominent grass aroma because of hexanal, while Shizhuhong chili oil, Denglongjiao chili oil, Erjingtiao chili oil, and Zhoujiao chili oil had a prominent floral aroma because of 2, 3-butanediol. Chili oils could be well divided into 3 groups by the partial least squares discriminant analysis (PLS-DA). According to the above results, the 10 kinds of chili oil had their own characteristics in color, capsaicinoids and flavor. Based on quantitative physicochemical indicators and flavor substances, the theoretical basis for the compounding of chili oil could be provided to meet the production demand more scientifically and accurately.
研究领域:
食品科学
·
化学分析
·
感官科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Shuo Zou,Yiren Zhang,Qiaojun Wang,Lixue Yang,Emad Karrar,Qingzhe Jin,Hui Zhang,Gangcheng Wu,Xingguo Wang
Beef tallow (BT) is the common hotpot oil used in Sichuan hotpot, increasing its characteristic flavors and making it taste better. However, the cholesterol content in BT is high, which may induce cardiovascular diseases. In this study, the effect of palm stearin (PS) on Sichuan hotpot oil was evaluated. The PS: BT blends showed similar physicochemical properties to BT from the results of sensory evaluation, pulsed NMR, DSC, and polar light micrograph (PLM). Furthermore, since spiciness is the essential characteristic of Sichuan hotpot, the digestive properties of capsaicinoids in hotpot oil were used as an evaluation index. The results showed that the digestive properties of capsaicinoids in hotpot oil containing PS were consistent with those without PS. In conclusion, PS can be partially used to replace BT, which can broaden the types of oil used for hotpot and help develop a new hotpot oil.
研究领域:
食品科学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Junkui Li,Chen Li,Wang Guo,Yiqing Guo,Xiaobo Zou,Zongbao Sun
The development of a capsaicin detection system that integrates facile preliminary evaluation with high sensitivity and accuracy across a wide detection range is crucial for the discrimination of gutter oil and the protection of human health. In this study, we constructed a novel colorimetric/SERS dual-mode detection system utilizing peroxidase-like Au@Pt nanoparticles (NPs) that exhibit SERS-activity. The Au@Pt NPs feature a spherical gold nanoparticle core with synapse-like platinum nanoparticles distributed intermittently on their surface, this structure enhances the catalytic activity of the PtNPs and the SERS activity of the AuNPs, making them ideal for designing colorimetric/SERS dual-mode probes. Based on the colorimetric assay, we achieved a capsaicin detection range from 1 to 10,000 μM, with a limit of detection (LOD) of 3.48 μM, as determined by absorption spectroscopy analysis. Additionally, a linear calibration curve for capsaicin ranging from 10 −9 to 10 −5 M was established using the SERS method, yielding an LOD of 7.38 nM. By integrating the advantages of facile detection through colorimetric analysis with the high-sensitivity trace assay provided by SERS, this proposed dual-mode detection system demonstrates excellent sensitivity, reliability, selectivity, and wide detection range compared to previous probes and methods. Consequently, this method can serve as an efficient tool for monitoring oil quality.
研究领域:
分析化学
·
食品安全
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2024
·
IF 5.3
Rui Wang,Pei-Feng Guo,Jing Nie,Xing Hu,Yu-Wei Wu,Shu Zhu,Bo-Ru Chen,Jian Li,Xin-An Zeng,Fei-Yue Xu
In this study, the inhibitory activity and mechanism of three gingerols (6-, 8- and 10-gingerol) on xanthine oxidase (XOD), a key enzyme responsible for the formation of uric acid, were evaluated. The three gingerols reversibly inhibited XOD activity in a mixed manner with IC 50 values of (13.16 ± 0.41), (10.18 ± 0.22) and (6.88 ± 0.11) μM, respectively. 10-gingerol exhibited stronger inhibitory ability, which might contribute to its superior antioxidant properties, higher binding affinity and more significant conformational changes for XOD compared to other gingerols. Fluorescence quenching and molecular docking results indicated that gingerols acted in the vicinity of the flavin adenine dinucleotide in XOD, interacting with the surrounding key amino acid residues through hydrogen bonding and van der Waals forces, thereby preventing substrate entry and the release of catalytic products. Molecular dynamics simulations showed that the addition of gingerol decreased the stability and increased the structural density of XOD. These findings could provide valuable data for the application of gingerols in new XOD inhibitors and hypouricemic functional foods.
研究领域:
生物化学
·
药理学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
DESALINATION
·
2023
·
IF 9.9
Li Xu,Zhibin Pang,Hongyan Yu,Mengyao Guo,Xuefeng Yan,Xiaohui Jiang,Liangmin Yu
Loose nanofiltration membranes (LNMs) are considered promising for dye/salt separation. However, LNM real-world applications are still hindered by membrane fouling . Here, we report the fabrication and relevant properties of novel antifouling LNMs, made by the codeposition of N,N′-((4,5,6-trihydroxy-1,3-phenylene) bis (methylene)) diacrylamide (AMTHBA) and polydopamine (PDA) onto polysulfone (PSf) via Michael addition and Schiff base processes. The introduction of the capsaicin-mimic AMTHBA, which has hydrophilic and antimicrobial properties, can markedly enhance the looseness of the selective layers, increasing the permeability and antifouling capacities of LNMs. The LNM dye/salt separation performance and surface microstructure can be adjusted by varying AMTHBA addition and codeposition time. The optimum PSf/PDA-AM 1.5 LNM achieved ultrahigh water permeance (72.21 L m −2 h −1 bar −1 ), excellent dye rejection (e.g., 98.69 % Congo Red) and low salt retention (e.g., 6.32 % NaCl). Notably, it also exhibited outstanding antifouling properties towards organic foulants (flux recovery ratio exceeded 98 % after 2 fouling/backwash cycles) and bacteria (sterilizing ratio of E. coli and S. aureus in aqueous solution was 87.53 % and 81.51 %). Furthermore, PSf/PDA-AM LNM maintained stable separation performance in 30 h dye/salt filtration test, and desirable retention efficiency during 12,000 ppm × h chlorination. This work provides opportunities for developing high-performance antifouling LNMs for textile wastewater treatment .
研究领域:
材料科学
·
环境工程
·
膜技术
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2017
·
IF 3.649
Qilong Wang,Qiuyu Wei,Qiuxuan Yang,Xia Cao,Qiang Li,Feng Shi,Shan Shan Tong,Chunlai Feng,Qingtong Yu,Jiangnan Yu,Ximing Xu
[6]-Gingerol, one of the components of the rhizome of Ginger, has a variety of biological activities such as anticoagulant, antioxidative, antitumor, anti-inflammatory, antihypertensive, and so forth. However, as one of the homologous phenolic ketones, [6]-gingerol is insoluble in water which limits its applications. Herein, we prepared [6]-gingerol proliposomes through modified thin-film dispersion method, which was spherical or oval, and physicochemically stable with narrow size distribution. Surprisingly, in vitro release of [6]-gingerol loaded proliposome compared with the free [6]-gingerol was significantly higher and its oral bioavailability increased 5-fold in vivo. Intriguingly, its antitumor effect was enhanced in the liposome formulation. Thus, our prepared [6]-gingerol proliposome proved to be a novel formulation for [6]-gingerol, which significantly improved its antitumor effect.
研究领域:
药剂学
·
肿瘤学
·
天然产物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Processes
·
2025
·Vol. 13
·期 7
·2149
·
IF 2.8
Yang Zhang,Siyi Yang,Wensi Li,Xiaoyan Li,Xiangqin Lai,Xiang Li,Wuwan Xiong,Bo Zhang
This study employed high-pressure ultrasonic-microwave-assisted extraction (HP-UMAE) to extract gingerols from ginger. The extraction yield and total polyphenol content of the extracts were determined. Their antioxidant activity was assessed by DPPH and ABTS radical scavenging assays, and compared with extracts obtained by leaching extraction, reflux extraction, ultrasonic-assisted extraction (UAE), microwave-assisted extraction (MAE), and ultrasonic-microwave-assisted extraction (UMAE). The results demonstrated that HP-UMAE achieved the highest extraction yield and the strongest ABTS radical scavenging activity among the evaluated methods. Furthermore, HP-UMAE extracts exhibited the highest concentrations of key gingerol constituents: 6-gingerol (14.29 mg/L), 8-gingerol (0.38 mg/L), 10-gingerol (1.95 mg/L), and 6-shogaol (4.32 mg/L). This enhanced efficacy is attributed to the synergistic combination of ultrasonic cavitation and microwave-induced thermal effects under elevated pressure. This synergy creates conditions promoting cellular wall disruption, facilitating the release of intracellular components, while concurrently enhancing solvent penetration and gingerol solubility. Scanning electron microscopy (SEM) analysis confirmed the significant structural damage inflicted on ginger cell walls following HP-UMAE treatment. The process parameters for HP-UMAE were optimized using single-factor experiments. The optimal extraction conditions were determined as follows: microwave power 800 W, ultrasonic power 1000 W, liquid-to-solid ratio 55:1, and temperature 100 °C (corresponding pressure 2 MPa). Under these optimized parameters, the extraction yield and ABTS radical scavenging rate reached their peak performance, yielding values of 4.52% and 43.23%, respectively.
研究领域:
食品科学
·
植物化学
·
分析化学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 53
·33219-33234
·
IF 3.361
Zhen Wu,Ruiping Gao,Hong Li,Yongde Wang,Yang Luo,Jiang Zou,Bo Zhao,Shiqi Chen
The combinatorial use of dietary jujube (Ziziphus jujuba) and ginger play a critical role in traditional Chinese medicines, folk medicine and dietary therapy. Joint effects were investigated from the viewpoint of the antioxidant (scavenging DPPH˙) and antitumor activities (against SW620 cells) of jujube polysaccharides and ginger 6-gingerol (G6G) alone and in combination. Jujube polysaccharides were extracted, purified, and characterized, and then their inhibiting and apoptotic effects alone and in combination with G6G were evaluated by the cytological tests, including Cell Counting Kit-8, colony-forming, Annexin V-FITC and propidium iodide, TdT-mediated dUTP nick end labeling (TUNEL) staining, and cell cycle assays. Results showed that the purified polysaccharide fraction (ZJPs-II) with average molecular weight of 115 kDa consisted of arabinose, rhamnose, glucose, xylose, and galactose. ZJPs-II and G6G alone dose-dependently scavenged DPPH˙ and inhibited the proliferation of SW620 cells, while their combination showed synergistic interactions (all combination index < 1). The studies further demonstrated that ZJPs-II and G6G alone reduced the cell colony-formation, induced apoptosis and arrested the cell-cycle at G2/M phase, while their combination achieved better effects and significantly arrested the growth at the G0/G1 phase. Collectively, our findings suggest enhancing the intake of jujube polysaccharides and G6G in a combinatorial approach for maintaining health and preventing cancer.
研究领域:
药理学
·
肿瘤学
·
营养保健品
·
传统中药
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FITOTERAPIA
·
2023
·
IF 3.4
Yajing Wang,Tao Zhang,Jie Liu,Xianfeng Huang,Xiaojing Yan
The clinical treatment of ulcerative colitis (UC) faces great challenges due to lifetime medication. In this study, Gingerol oil was extracted and purified by the process easily scale-up and cost effective, with productivity 2.72 ± 0.38% ( w /w, versus crude drugs). The quality control of gingerol oil was fully established by HPLC fingerprint with 4 common peaks identified as 6-gingerol, 8-gingerol, 6-shogaol and 10-gingerol. The similarities of 6 batches of gingerol oil are within 0.931–0.999. The protective effects of gingerol oil are equivalent to or even stronger than that of 6-gingerol on inflammation and oxidative stress of HT-29 cells induced by lipopolysaccharide and H 2 O 2 , as well as on UC in mice caused by dextran sulfate sodium salt (DSS). Our research conclusions coincide well with the holistic view of Traditional Chinese Medicine and network pharmacology . The absorption kinetics of gingerol oil were conducted using the in situ intestinal perfusion in rats and comparable absorption were achieved in the jejunum , ileum and colon segments within 2 h. Thus, gingerol oil colon targeting pellets were prepared by extrusion-spherization technique. The cumulative dissolution behaviors and mechanisms were observed and analyzed by fitting to dissolution model. Our studies provided reliable theoretical and experimental support for the gingerol oil as reliable therapeutic choice of UC.
研究领域:
药理学
·
胃肠病学
·
传统中药
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）