JOURNAL OF DAIRY SCIENCE
·
2020
·
IF 3.333
Suhua Wang,Bolong Fang,Meifang Yuan,Zexiang Wang,Juan Peng,Weihua Lai
ABSTRACT In this study, a novel colorimetric and fluorescent dual-mode ELISA based on glucose oxidase (GOx)-triggered Fenton reaction was developed for the qualitative and quantitative detection of danofloxacin (DAN). In this system, streptavidin-linked biotinylated anti-DAN-monoclonal antibody (SA-Bio-mAb) and biotinylated GOx (Bio-GOx) form the immune complex mAb-Bio-SA-Bio-GOx. In the absence of DAN, the mAb-Bio-SA-Bio-GOx would be immobilized by combining with coated DAN-BSA and catalyzed glucose to generate H 2 O 2 . The Fenton reaction between H 2 O 2 and Fe 2+ generated hydroxyl radicals, which oxidized the o -phenylenediamine to 2,3-diamino-phenazine. A dual-signal immunoassay with colorimetry and fluorescence as the signal readout was established. In the presence of DAN, DAN and DAN-BSA competed with Bio-mAb, decreasing the connection between immune complexes and DAN-BSA and finally resulting in lower signal of colorimetry and fluorescence. Under optimal conditions, the limit of detection of the fluorescence immunoassay was 0.337 ng/mL and was 5.24-fold lower than that of traditional ELISA. The colorimetric immunoassay cut-off value was 30 ng/mL in milk. The average recoveries of the method for milk samples that are spiked with different concentrations of DAN were 91.1 to 128.3%, with a coefficient of variation of 0.7 to 8.2%. These results of the method exhibited good agreement with those of liquid chromatography-tandem mass spectrometry system (LC-MS/MS) method. In brief, this work provides an improved screening strategy with high sensitivity and accuracy for the qualitative or quantitative detection of DAN in milk monitoring.
研究领域:
分析化学
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食品安全
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生物化学
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兽医学
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PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Huanhuan Bao,Meifang Yuan,Chengui Xiao,Daofeng Liu,Weihua Lai
A signal-enhanced LFIA based on tyramine (TYR)-induced AuNPs aggregation has been developed for the sensitive detection of danofloxacin (DAN). In the model, the hydroxyl radical produced by HRP catalyzing H 2 O 2 can trigger the TYR-AuNPs to aggregate on the T or C line for enhancing the detection signal. The linear range of TYR-AuNPs LFIA was 0.25–5 ng mL −1 with the limit of detection (LOD) of 0.032 ng mL −1 , and the LOD was 8-fold lower than that of the traditional AuNPs LFIA (0.26 ng mL −1 ). The TYR-AuNPs LFIA could be used with the naked eyes to qualitatively detect DAN with a cut-off limit of 2.5 ng mL −1 , which was 4-fold lower than that of the traditional AuNPs LFIA (10 ng mL −1 ). The recoveries of TYR-AuNPs LFIA were 86.04–105.14% and 92.41–110.19%, with the coefficient of variation of 1.71–2.05% and 4.42–5.89% in chicken and pork, respectively.
研究领域:
分析化学
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纳米技术
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食品安全
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PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Xiang Li,Lei Jia,Yongxin Li,Bing Bi,Lina Zhang,Jun Xu,Tongqian Zhao
The residues of widely used fluoroquinolone antibiotics (FQs), including danofloxacin (DNX), enrofloxacin (ENR), norfloxacin (NFX), and pefloxacin (PEF), may accumulate in humans through contaminated water/food, posing health risks. Therefore, the rapid detection of FQs is of great research significance. In light of this, Tb/CdTe QD probes were developed in this study for the quantitative detection of multiple FQs, and a logic gate sensor array was constructed based on this probe to enable qualitative differentiation of these antibiotics. The probe possessed excellent anti-interference capability and remarkable stability. In real samples detection, such as lake water, tap water, milk, honey, and eggs, high recovery rates were successfully achieved. The detection limits for DNX, ENR, NFX, and PEF were 3.69 nM, 6.56 nM, 8.70 nM, and 4.72 nM, respectively. Furthermore, by integrating this ratio fluorescent probe with the color recognition software on a smartphone, visual semi-quantitative detection of FQs was successfully realized.
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ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Jing Xu,Si-Heng Luo,Chen-ru Xiong,Jia-hao Liu,Zeyu Zhao,Wei-qi Lin,Wei Zhang,Ping Guo,Cheng Pan,Quanlong Li,Zhong-Qun Tian,Bin Ren,Guo-Kun Liu
The overused quinolone antibiotics in animal husbandry and clinical medicine pose a growing threat for global health as they enter ecosystem via agricultural discharge and medical wastewater. Consequently, risk assessment for environmental and human exposure is highly demanded, which calls for an accurate, reliable and rapid qualitative and quantitative analysis of trace antibiotics in different matrices. Surface-enhanced Raman spectroscopy (SERS), providing fingerprint chemical information with near-single-molecule sensitivity, has received considerable attention. Herein, considering the common physicochemical properties of various quinolone antibiotics, we first developed sample pretreatment strategy consisting of solid-phase extraction (SPE), liquid–liquid extraction (LLE), salting-out assisted liquid–liquid extraction (SALLE), and NPs purification (NPs (p)). The modular SPE–LLE–SALLE–NPs (p) coupling with spectral separate network (SSNet) (a deep-learning based spectral unmixing algorithm) enabled a rapid (<8 min per sample), sensitive (μg L–1), on-site, and intelligent SERS analysis of 19 quinolone antibiotics in food, biological, and environmental matrices. Besides, the identification of each QN in a five-target mixture sample enabled SPE–LLE–SALLE–NPs (p)–SSNet–SERS to be a highly promising rapid detection strategy in blind sample screening qualitatively and quantitatively. The integrated SPE–LLE–SALLE–NPs (p)–SSNet–SERS offers a cross-media, full-process, and big data-enhanced rapid monitoring solution for antibiotic pollution within the “source-pathway-sink” framework.
研究领域:
环境科学
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分析化学
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光谱学
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化学中的人工智能
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食品安全
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药物污染
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深度学习应用
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水质监测
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抗生素耐药性
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同一健康
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高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2026
·
IF 9.6
Zhiming Chen,Jiabin Chen,Haoming Bao,Kaitao Liu,Pan Zeng,Fan Yang,Wei Cao,Dongqi Yang,An Cao,Tao Zhang,Yue Li
Surface-enhanced Raman scattering (SERS) has been one of the efficient strategies for trace molecular analysis, but the traditional substrates with sharp tips cannot effectively detecting the molecular isomers. Here, we develop a facile way to fabricate large-area, reproducible Ag/Au nanocavity arrays by the combination of microsphere-templated soft lithography and self-assembly. Compared to the flat and convex substrate, the Ag/Au units arranged on the concave surface exhibit a high-density of hotspots via spatial light confinement and multi-reflection-enhanced electromagnetic coupling, which enables highly sensitive detection of probe molecules down to10 −12 M. Moreover, by integrating machine learning algorithms, the test set shows a coefficient of determination (R 2 ) of 0.9896, achieving advanced training performance for classifying four structurally analogous quinolone antibiotics (ciprofloxacin, enrofloxacin, danofloxacin, and enoxacin) with a quantitative prediction of 10 −10 M. This work demonstrates a robust pathway for the controllable fabrication of high-performance SERS substrates for the development of intelligent, quantitative, and on-site sensing platforms in complex practical scenarios. Download: Download high-res image (179KB) Download: Download full-size image
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高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Guangxin Yang,Jingru Zhang,Junyu Zhang,Peng Wang,Wei Xia,Jun Wang,Xiaosheng Shen,Cong Kong
Antibiotic use in aquaculture leads residues in aquatic products, contributing to antimicrobial resistance and potential human toxicity. Complex sample matrices hinder detection. This study uses wolfberry biomass waste-derived biochar as a novel solid-phase extraction (SPE) sorbent to detect 26 antibiotic residues (14 fluoroquinolones, 12 sulfonamides) in aquatic products, effectively repurposing agricultural waste into a high-performance, eco-friendly material. The mesoporous structure of the biochar enables rapid diffusion of antibiotics into its interior, facilitating highly efficient adsorption. Adsorption kinetics follow a pseudo-first-order model, reaching equilibrium in 60 min. The superior adsorption performance enables the prepared biochar to serve as an SPE sorbent for drug residue analysis with stable performance over at least three reuse cycles. The SPE was used for real samples analysis based on HPLC-HRMS, and the detection limits was 1–4.5 μg·kg −1 , recoveries was 75–119 %. Validation against the national standard GB 31658.17–2021 confirms method practicality and reliability.
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高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2017
·Vol. 409
·期 19
·4559-4569
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IF 3.431
Xu Jia,Li Yunfang,Li Cao,Zhang Rui,Xiao Yuxiu
A hexafluoroisopropanol (HFIP)-mediated cloud point extraction (CPE) system was established. A small amount of HFIP (even 1%, v / v ) can dramatically reduce the cloud point of Triton X-100 (TX-100) aqueous solution (even to 1 °C) and make liquid-liquid two-phase separation (coacervate phase and aqueous phase) occur at room temperature over a wide range of TX-100 concentration (0.5∼10%, g/mL). HFIP-mediated coacervate phase has smaller volume (volume ratio is 1.8∼8.9% relative to the volume of the total solution with 1∼5% TX-100) and larger micelle aggregates (30∼80 nm in diameter) compared to temperature-induced coacervate phase (volume ratio at 2.8∼14.0%, the diameter of micelle aggregates at 5∼30 nm). HFIP-mediated CPE was coupled to high-performance liquid chromatography with ultraviolet detection (HPLC-UV) for the extraction and detection of organic pollutants in water, namely, polycyclic aromatic hydrocarbons (PAHs), fluoroquinolones (FQs), and sulfonamides (SAs) with different polarities, charges, and hydrogen-bonding properties. HFIP-mediated CPE provides much higher extraction rates (ERs) and enrichment factors (EFs) for FQs (91∼106%, 50∼59), PAHs (63∼90%, 33∼49), and SAs (26∼55%, 16∼34) compared with the temperature-induced one (ERs: 4∼8% for FQs, 25∼46% for PAHs, and 4∼37% for SAs; EFs: 1∼3 for FQs, 6∼12 for PAHs, and 8∼13 for SAs). The limit of detection ranges from 0.24 to 0.33 ng/mL for FQs, 0.04 to 0.38 ng/mL for PAHs, and 0.63 to 1.31 ng/mL for SAs. The proposed method was applied in the analysis of real water samples, and the recovery of 79.4∼110.8% and the relative standard deviation of 0.2∼16.3% were achieved for the three types of pollutants.
Schematic illustration of HFIP-mediated cloud point extraction
研究领域:
环境科学
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分析化学
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PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Di Zhao,Yanmei Shi,Chenqing Zhang,Zhihong Chen,Weixia Zhu,Shusheng Zhang,Kai Hu
A zwitterionic covalent organic framework was synthesized and applied as SPME blade coating for the extraction of fluoroquinolones in lake water and milk samples. The structure and functional groups of the sorbent were characterized via SEM, XPS, FT-IR, and XRD. Adsorption kinetic/isotherm analyses demonstrated chemisorption-driven monolayer adsorption with high affinity. Under the optimal SPME conditions, a high-throughput extraction method was established by incorporating zwitterionic covalent organic framework-coated blades into a 24-well plate system, which was subsequently coupled with LC-MS/MS for analysis. The proposed method exhibited good linearity (0.01–10 ng mL −1 , R 2 ≥ 0.9938), achieving LODs of 0.1–2.5 pg mL −1 in lake water and 0.2–2.8 pg mL −1 in milk, while recoveries were in the range of 72.3 %–124.7 % with RSD < 10.3 %. This approach provides an effective strategy for monitoring trace fluoroquinolones in environmental and food matrices, showcasing the potential of DBS-EB-COF-enhanced high-throughput SPME systems for applications in environmental and food safety surveillance.
研究领域:
分析化学
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环境科学
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食品安全
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材料科学
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药物分析
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高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Chang Liu,Yuan Chen,Hong Lin,Limin Cao,Kaiqiang Wang,Xiudan Wang,Jianxin Sui
ABSTRACT Low specificity and sensitivity often restrict the fluoroquinolones (FQs) detection when traditional IgG antibodies are used as recognition elements in immunoassay. Herein, shark-derived single-domain antibodies (ssdAbs) were used as new recognition elements to enhance the sensitivity and specificity for norfloxacin detection in immunoassay. Firstly, NOR-specific clones were isolated by phage display technology. Finally, five clones were successfully isolated from a NOR-immunized library. Secondly, a soluble expression system was constructed to produce ssdAbs. The 1N9 ssdAb exhibits the best affinity to the NOR complete antigen, with a limit of detection of 0.392 ng/mL and the cross-reaction rates of less than 8.240%, indicating its high sensitivity and specificity as a new recognition element in NOR detection. More importantly, the ELISA result proved that in NOR-negative fish matrix, no serious interference was observed, the 1N9 ssdAb could still recognize complete antigen NOR-OVA effectively. While in icELISA, 1N9 ssdAb could recognize NOR in different NOR-spiked concentrations between 10 to 1000 ng/mL, with the recovery rates between 93.66 - 116.49% with high reproducibility. Our study provides a new and effective recognition element with great potential for NOR detection.
研究领域:
免疫学
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分析化学
·
药学
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高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Chang Liu,Yuan Chen,Hong Lin,Limin Cao,Kaiqiang Wang,Xiudan Wang,Jianxin Sui
Background Due to the serious issue of ofloxacin (OFL) abuse, there is an increasingly urgent need for accurate and rapid detection of OFL. Immunoassay has become the “golden method” for detecting OFL in complex matrix beneficial to its applicability for a large-scale screening, rapidity, and simplicity. However, traditional antibodies used in immunoassay present challenges such as time-consuming preparation, unstable sensitivity and specificity, and difficulty in directional evolution. In this paper, we successfully developed an OFL detection method based on a shark-derived single-domain antibody (ssdAb) to address these issues. Results Using phage display technology and a heterologous expression system, OFL-specific clones 1O11, 1O13, 1O17, 1O19, 1O21, and 2O26 were successfully isolated and expressed in soluble form. Among all OFL-specific ssdAbs, the 1O17 ssdAb exhibited the highest binding affinity to OFL in a concentration-dependence manner. The limit of detection (IC 10 ) of 1O17 ssdAb was calculated as 0.34 ng/mL with a detection range of 3.40–1315.00 ng/mL, and its cross reactivity with other analogs was calculated to be less than 5.98 %, indicating high specificity and sensitivity. Molecular docking results revealed that 100Trp and 101Arg located in the CDR3 region of 1O17 ssdAb were crucial for OFL binding. In fish matrix performance tests, the 1O17 ssdAb did not demonstrate severe matrix interference in OFL-negative fish matrix, achieving satisfactory recovery rates ranging from 83.04 % to 108.82 % with high reproducibility. Significance This research provides a new and efficient OFL detection recognition element with significant potential in immunoassay applications, broadening the application scenarios of ssdAbs. It offers valuable insights into the structure-activity relationship between ssdAbs and small molecules, laying a theoretical foundation for the further directional modification and maturation of ssdAbs in subsequent applications.
研究领域:
分析化学
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分子生物学
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免疫学
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药理学
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PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2024
·
IF 4
Wenrui Li,Yike Dang,Ruihong Wang,Weizhong Jin,Xiangli Chen,Yafei Zhai,Zhuoqun Su,Guoliang Li
Fluoroquinolones (FQs) are widely used to treat various animal diseases. The high residue of veterinary drugs in animal-origin food are new pollutants that seriously threaten human health. In this work, Fe 3 O 4 @LDH/ZIF was prepared by the in situ growth of zeolitic imidazolium skeletons (ZIFs) on Fe 3 O 4 @Zn/Al-layered double hydroxides (Fe 3 O 4 @Zn/Al-LDH), which was used as the adsorbent of FQs for magnetic solid phase extraction (MSPE). The prepared materials were characterized by using FT-IR, XRD, TEM, SEM, VSM, zeta, and nitrogen adsorption-desorption analyses. Under the optimized conditions, the limit of detections (LODs) of proposed methods for FQs is determined as low as 0.1–0.6 μg·kg −1 with good linearity (R 2 ≥0.9972). Furthermore, the MSPE approach has been effectively utilized to extract of FQs from a range of meat varieties including fish, pork, chicken, bullfrog, and beef. The recoveries from the spiked samples ranged from 86.5 % to 107 %, with relative standard deviations (RSD) below 8.8 %. These results demonstrated that the prepared nanoparticles hold unique advantages for trace FQs analysis in foodstuffs. This study provides a magnetic adsorbent for food sample pretreatment, which can rapidly enrich compounds similar in structure to fluoroquinolones.
研究领域:
食品科学
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分析化学
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纳米材料科学
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DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Ruoqin Jia,Lei Jia,Xiaolei Zhao,Yuanyuan Huang,Lina Zhang,Dan Zhao,Jun Xu,Tongqian Zhao
Sensitive and selective detection of fluoroquinolones, especially from different sources, is challenging. This study reported an uncommon three-cage lanthanide metal-organic framework ( 1-Eu ) with a Eu 3+ cluster as its structural primitive using a C 2 -symmetric 5,5-(Pyrazine-2,6-diyl) diisophthalic acid ligand. The 1-Eu probe effectively detected four fluoroquinolones through distinct color changes and spectral emission bands, demonstrating excellent performance with low detection limits: moxifloxacin (LOD: 9.3 nM), danofloxacin (LOD: 33.7 nM), gatifloxacin (LOD: 67.9 nM), and ofloxacin (LOD: 238.6 nM). Mechanistic studies revealed that internal filtration and photoinduced electron transfer (a-PET) effects were key factors. Furthermore, 1-Eu was successfully used to detect fluoroquinolones in food samples. Additionally, portable paper-based sensors were developed to quickly semi-quantify analyte concentrations using a smartphone color recognition app, underscoring the practical potential of this probe. This study introduces a novel methodology for the identification and detection of fluoroquinolones to enhance food safety.
研究领域:
食品安全
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分析化学
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材料科学
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PubMed
高影响因子
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