SENSORS AND ACTUATORS B-CHEMICAL
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2023
·
IF 8.4
Yiyu Chen,Huiting Lian,Bin Liu,Guangming Liu,Xiaofeng Wei
Food allergy diagnosis requires a highly sensitive detection of allergen-specific immunoglobulin E (sIgE) in the serum. Enzyme-linked immunosorbent assay (ELISA) is a classical method widely used for sIgE detection but had to rely on costly and time-consuming assay procedures, skilled technicians, and bulky equipment. Here, a multiplexed origami paper analytical device (moPAD) that is hexahistidine-tagged and integrated with a nitrilotriacetic acid-functionalized surface with nickel (Ni-NTA) was developed to achieve low-cost and high-efficiency immunosensing of multiple allergen-specific IgEs in serum. The major oyster allergens, Cra a 1, Cra a 2, and Cra a 4, were used to detect sIgE in human serum. The feasibility of the moPAD-based immunosensing strategy was successfully demonstrated by desirable sensitivities for multiple targets. The detection results of moPAD for 72 serum samples were 100% consistent with clinical test results, while 69 of these results showed no significant differences from ELISA results, demonstrating the accuracy and stability of this method. A fully integrated moPAD reduces detection time and achieves highly sensitive quantification using a simple smartphone-based readout. Efficient allergen modification of paper devices has great applicability in allergen component-resolved diagnostics. The implementation of moPAD can make the process of allergy diagnosis simpler for patients, physicians, and healthcare systems alike.
研究领域:
免疫学
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生物医学工程
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诊断技术
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过敏研究
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产品关联
DOI
高影响因子
International Journal of Environmental Research and Public Health
·
2013
·Vol. 10
·期 7
·2897-2905
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IF 1.998
Juan Peng,Shanshan Song,Liguang Xu,Wei Ma,Liqiang Liu,Hua Kuang,Chuanlai Xu
We have established a highly sensitive sandwich enzyme-linked immunosorbent assay (ELISA) based on two monoclonal antibodies (mAb) to measure the content of the major peanut allergen Ara h 1 in foods. Two mAbs were selected out of 12 murine hybridoma cells secreting Ara h 1-specific antibody. Using mAb 6 as the capture antibody and HRP-labelled mAb 4 as the detection antibody, the limit of detection (LOD) the assay was 0.34 ng/mL. Cross-reaction analysis showed that this method was strongly specific and had no cross-reactions with Ara h 2, pea protein or soy protein. Sample analysis showed that this ELISA was a useful tool to monitor peanut allergens in food products by measuring Ara h 1 content.Keywords:peanut allergen;Ara h 1;monoclonal antibody;sandwich ELISA
研究领域:
食品科学
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免疫学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Jiahui Li,Yuanyuan Zhang,Zhengshun Wen,Jiangbing Shuai,Xiaofeng Zhang,Jinru Zhou,Aiqian Ye,Linglin Fu
The delayed reporting of milk allergen residue detection results is a significant factor contributing to substantial losses for food production enterprises. Cumbersome operational procedures and high testing costs often lead to the abandonment of tests, resulting in frequent allergic reactions among milk-sensitive populations. Therefore, there is an urgent need for a novel, rapid, and low-cost detection method to mitigate enterprise losses and protect allergic individuals. This study investigates the formation mechanism of convection PCR, driven by Fast Taq polymerase in standard PCR tubes, and develops a fluorescence detection device for convection PCR. Through molecular docking and experimental validation of the trimmed β-LG aptamers, we elucidate their potential multi-site binding mechanism. Utilizing this mechanism, we designed a dual-strand hybridization magnetic bead detection system (dsA-SA-B), employing easily synthesized and amplified aptamer complementary strands as an indicator signal; Signal amplification was achieved within 15 min, while the entire detection process was completed in less than 50 min. This detection method allows for the rational selection of aptamer sequences based on actual testing needs, enabling the detection of hydrolyzed milk powder. The method demonstrated a linear detection range for β-LG from 5 to 100 μg/mL, with a detection limit as low as 0.25 μg/mL, effectively identifying hydrolyzed milk powder. This approach transforms protein detection into nucleic acid detection, and lays a solid foundation for multiplex PCR detection of eight major food allergens, facilitating the widespread detection of milk allergens and ultimately contributing to improved food safety and public health.
研究领域:
分子生物学
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食品安全
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免疫学
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生物技术
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分析化学
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DOI
高影响因子
Food Analytical Methods
·
2022
·Vol. 15
·期 6
·1535-1544
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IF 3.498
Li Linfang,Zhou Jinru,Wang Kuiwu,Chen Xiaojing,Fu Linglin,Wang Yanbo
Tropomyosin (TM), known as the major allergen in shellfish, has gained increasing concerns in industrialized countries, resulting in an urgent need for an accurate, simple, and sensitive method for TM detection in food products. Nucleic acid aptamers, short single-stranded oligonucleotides chains, can bind to various targets with a high affinity and specificity and hence have the potential to be used for the accurate detection of targets. Here, we proposed capillary electrophoresis-systematic evolution of ligands by exponential enrichment (CE-SELEX) for rapid selection and identification of specific aptamers against shellfish TM. A selected aptamer IX exhibited high specificity and high binding affinity against TM with K d determined (0.95 nM). In addition, the aptamer IX has great potential to be employed in the establishment of various aptasensors for TM detection in shellfish products. Furthermore, the CE-SELEX method may provide a universal and convenient method for the aptamer selection against more targets.
研究领域:
食品科学
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分子生物学
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产品关联
DOI
高影响因子
Food Bioscience
·
2023
·
IF 5.2
Jialin Hu,Xinxin Xu,Liguang Xu,Hua Kuang,Chuanlai Xu,Lingling Guo
Wheat gliadin is one of the most important causes of gluten sensitivity. This study aimed to develop a sandwich enzyme-linked immunosorbent assay (ELISA) and gold nanoparticle-based lateral flow (LFIA) strips to detect wheat allergen in food. Through cell screening, ten monoclonal antibodies (mAbs) against gliadin and after optimization were obtained, a pair of mAbs suitable for its detection (capture antibody: mAb 7; detection antibody : HRP-labeled mAb 6) were identified. Based on two of these antibodies, a sandwich ELISA with limit of detection (LOD) of 60 ng/mL in negative milk, and more importantly, negligible cross-reactivity to other allergens was developed. The average recoveries for gliadin in negative milk were 99.16%–100.07% using the sandwich ELISA. We also developed LFIA strips for the rapid detection of gliadin with visual limit of detection (vLOD) of 25 ng/mL and calculated LOD value of 6.56 ng/mL in negative milk. The test results of positive samples obtained from the LFIA strips were highly consistent with those of the sandwich ELISA. Thus, the developed LFIA strip is an effective and reliable tool for the rapid and on-site detection of wheat allergen in milk.
研究领域:
食品科学
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免疫学
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产品关联
DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2024
·
IF 3.1
HongXia Chen,Hao Zhou,Zhiwen Qi,Xingying Xue,ChengZhang Wang
Toxicodendron vernicifluum bark has been used for many years as a component in foods and as a traditional herbal medication. Unfortunately, the presence of urushiols, which induce allergies, limits its application. This study used a vortex-blending matrix solid-phase dispersion microextraction technique to extract urushiols from Toxicodendron vernicifluum bark. HPLC was used to evaluate the amounts of the extracted urushiols (15:0, 15:1, 15:2, and 15:3). The modified magnetic adsorbent was prepared through an in situ coprecipitation method and characterized using a variety of techniques. The optimized extraction conditions are as follows: using magnetic Zeolite Socony Mobil-Five as an adsorbent, a 1:2 sample/adsorbent ratio, 2.5 min of vortex-blending time, 4 mL of 0.1% (V/V) trifluoroacetic acid-methanol as the elution solvent and 8 min of ultrasound time. There was good linearity and high repeatability in the method. Furthermore, the limits of detection for the urushiols ranged from 0.20 to 0.50 μg/mL. Under the optimized conditions, 50 compounds were identified by ultra high performance liquid chromatography and quadrupole time-of-flight mass spectrometry. These compounds included 8 phenolic acids, 9 monomeric urushiols, 11 urushiol dimers, 10 other components, and 11 flavonoids. The suggested approach, which has the advantages of few stages and high extraction efficiency over existing extraction procedures, is a potentially useful method for obtaining and evaluating urushiols in raw materials or extracts.
研究领域:
植物化学
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分析化学
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药物化学
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产品关联
DOI
PubMed
高影响因子
Food Analytical Methods
·
2015
·Vol. 8
·期 10
·2605-2611
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IF 1.956
Peng Juan,Song Shanshan,Liu Liqiang,Kuang Hua,Xu Chuanlai
In order to develop highly sensitive immunoassays for detecting the major peanut allergen, Ara h 2, we prepared six monoclonal antibodies (mAb) with high affinity to Ara h 2. Using mAb 5 as a capture antibody and HRP-labeled mAb 1 as a detection antibody, a sandwich enzyme-linked immunoassay (ELISA) was established with a limit of detection (LOD) of 0.02 ng/mL. An immunochromatographic strip based on these two mAbs was also established with a LOD of 1 ng/mL. Both tests showed high specificity in cross-reactivity experiments. Sample analyses indicated that these two immunoassays were feasible, accurate, and highly sensitive for measuring allergen Ara h 2 of food.
研究领域:
食品科学
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免疫学
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生物化学
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产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2016
·
IF 2.857
Lingmei Li,Chengjian Wang,Shan Qiang,Jixiang Zhao,Shuang Song,Wanjun Jin,Bo Wang,Ying Zhang,Linjuan Huang,Zhongfu Wang
Glycosylation of many proteins has been revealed to be closely related with food allergies, and screening and structural analysis of related glycoproteins and glycoallergens are essential for studies in this field. Herein, we describe detailed N-glycoform analysis of all glycoprotein fractions of soybean protein isolate (SPI) separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) to disclose structural features of the glycan moieties of more soybean glycoproteins. SPI was fractionated by SDS-PAGE, and the generated protein bands were recovered and subjected to in-gel N-glycan release and labeling using a one-pot method newly developed by our group, followed by detailed analysis by electrospray ionization mass spectrometry (ESI-MS) and online hydrophilic interaction liquid chromatography coupled with electrospray ionization tandem mass spectrometry (HILIC-ESI-MS/MS). As a result, we found seven bands mainly containing oligomannose-type glycans; two mainly contain core α1,3-fucosylated glycans, and six have no glycans. This study is the first report that discovers core α1,3-fucosylated N-glycans in bands 1, 2, and 6 and discloses bands 3, 4, 5, and 7 as glycoproteins and their N-glycoforms. Therefore, it can expand our knowledge about soybean protein glycosylation and provide significant structural reference for research of soybean allergens.
研究领域:
食品科学
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蛋白质组学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2024
·
IF 4.9
Jinru Zhou,Yanbo Wang,Lei Zheng,Huan Li
Seafood allergy represents a critical public health challenge, necessitating the implementation of sensitive, precise, and quantitative detection methods for seafood allergens to guarantee accurate food labeling. Parvalbumin (PV) and tropomyosin (TM) are recognized as the primary allergens in fish and shellfish. Herein, we designed a fluorescence sensing system for the simultaneous detection of PV and TM that employs aptamer-initiated catalytic hairpin assembly (CHA). In this strategy, we utilized four hairpin dimers (H1, H2, H3, and H4) as foundational elements to create the sensing scaffolds. The CHA-based fluorescent aptasensor demonstrated an extensive linear range, ranging from 0.001 μg/mL to 100 μg/mL, with detection limits of 0.29 μg/mL for PV and 0.27 μg/mL for TM. This biosensor demonstrates exceptional selectivity and stability, in addition to good accuracy. Importantly, this biosensor has been effectively utilized to quantify PV and TM in four different commercial food products, achieving satisfactory accuracy. With these advantages, our proposed CHA-based aptasensor can offer a universal tool for sensitive and precise detection of food allergens in commercial foods throughout the supply chain.
研究领域:
生物化学
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食品安全检测
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分子诊断
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DOI
高影响因子
Toxics
·
2025
·Vol. 13
·期 5
·386
·
IF 4.1
Xiaolin Niu,Junji Wu,Yi Chen,Na Luo,Yanpeng Gao
Antimicrobial fragrances, commonly found in household and personal care products, are frequently detected in water bodies, yet their environmental fate and transformation mechanisms remain inadequately explored. This study investigates the photochemical transformation of cinnamaldehyde (CA), a representative antimicrobial fragrance, and its consequence for toxicological effects. The results showed that under UV irradiation, 94.6% CA was eliminated within 60 min, with a degradation rate of 0.059 min−1. Laser flash photolysis, quenching experiments, and electron paramagnetic resonance spectra identified O2•−and3CA*as the important species, contributing 29.4% and 33.6%, respectively, to the transformation process. Additionally, singlet oxygen (1O2), hydroxyl radicals (•OH), and solvated electrons (eaq−) were involved in mediating the oxidation reactions. These species facilitated photoionization and oxidation, resulting in the formation of five major transformation products, including cis-cinnamyl aldehyde, cinnamic acid, styrene, 1aH-indeno [1,2-b]oxirene), and 1-Oxo-1H-indene. Most of these products were persistent, and exhibited considerable ecotoxicological risks. Specifically, the cinnamic acid and 1-Oxo-1H-indene caused severe skin irritation, while cinnamic acid induced significant eye irritation. Notably, the transformation products demonstrated sensitizing effects on human skin. This study underscores the overlooked ecotoxicological risks associated with the photochemical transformation of antimicrobial fragrances, revealing their potential to generate potent allergens and other harmful byproducts.
研究领域:
环境科学
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毒理学
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化学
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
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2024
·
IF 4.8
Jialin Hu,Lingling Guo,Liqiang Liu,Liguang Xu,Hua Kuang,Chuanlai Xu,Xinxin Xu
α-Lactalbumin (α-La) is a common allergenic protein in dairy products, posing a potential hazard to human health. Therefore, there is an urgent need to develop an efficient and sensitive analytical method to assess the α-La content in food. After screening by cell fusion assay, we obtained a pair of monoclonal antibodies (mAbs) (mAb 1 and 9). The calculated limit of detection of the sandwich enzyme-linked immunosorbent assay (ELISA) was 3.99 ng/mL. By labeling mAbs with colloidal gold, we developed a sensitive and rapid immunochromatographic assay (ICA) strip method for the detection of α-La in dairy products. The established ICA strips showed high sensitivity and specificity for the detection of α-La, with a calculated limit of detection and a visual limit of detection of 10.33 and 50 ng/mL, respectively. The average recoveries for the determination of raw cow's milk and recombined milk using ICA strips ranged from 97.60% to 102.96%. The ICA strips showed good correlation with the high-performance liquid chromatography (HPLC) method when analyzing actual samples. The above results indicated that both methods can be used for the determination of α-La in dairy products.
研究领域:
食品安全检测
·
免疫学
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产品关联
DOI
高影响因子
Analytical Methods
·
2021
·Vol. 13
·期 27
·3059-3067
·
IF 2.896
Guohui Li,Yafeng Wei,Lizhou Ma,Yin Mao,Ran Xun,Yu Deng
β-Conglycinin, composed of three subunits (α′, α and β), is the main allergen of soy protein which can cause severe allergic reactions, such as diarrhea, decreased growth performance and even death. Among them, the β subunit is more stable and difficult to remove, being one of the main nutritional inhibitors, which can be used to evaluate the concentration of β-conglycinin. However, there is no effective, accurate method for its β subunit rapid detection. Herein, we have successfully selected a high affinity β subunit aptamer (Kd = 6.9 nM) and developed a highly sensitive aptasensor. The aptasensor displayed high specificity and the β subunit at a concentration of 70–350 nM could be detected with a detection limit of 4.48 nM (3S/N). In addition, the recoveries of β subunit were more than 90%, demonstrating its practical properties for complicated conditions such as food quality control and disease diagnosis, without requiring expensive and sophisticated equipment.
研究领域:
生物化学
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食品安全
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分子生物学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）