JOURNAL OF IMMUNOLOGY
·
2025
·
IF 3.4
Shi Zhaohui,Zhao Tianfeng,Wu Yongjin,Tang Zhiyuan,Wang Zaixing,Li Dingbo,Wang Chong
Allergic rhinitis (AR) is a respiratory airway disorder characterized by inflammation and barrier dysfunction. The transcription factor specificity protein 1 (SP1), a member of the Sp/Krüppel-like factor family, is known to be associated with inflammation. This study aimed to explore the impacts of SP1 on AR. An AR murine model was developed through multiple intranasal challenges with ovalbumin (OVA). Human nasal epithelial cells (HNEpCs) were cultured at the air–liquid interface (ALI) and treated with IL-13 to create a cell model with barrier damage. Bioinformation samples revealed that SP1 expression was upregulated in nasal epithelial cells of people with AR. High Sp1 expression was observed in AR mice nasal mucosa. Silencing Sp1 reduced nasal rubbing, sneezing, serum levels of IgE, histamine, and nasal swelling in AR mice. Additionally, Sp1 silencing inhibited goblet cell proliferation, eosinophilic infiltration, and levels of IL-4 and IL-13. Sp1 knockdown reversed the elevated fluorescein isothiocyanate–dextran 4 kDa permeability, nasal epithelial cell shedding, and decreased expression of E-cadherin and Claudin-1. In vitro, knocking down SP1 led to an increase in transepithelial electrical resistance and levels of E-cadherin and Claudin-1. Mechanistically, SP1 upregulated forkhead box C1 (FOXC1) expression through transcriptional activity, and FOXC1 overexpression reversed the protective effects of SP1 silencing on epithelial barrier damage. Overall, our findings suggest that SP1 aggravates AR, potentially through the transcriptional activation of SP1 to FOXC1.
研究领域:
免疫学
·
分子生物学
·
呼吸系统疾病
·
转录因子
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nutrients
·
2022
·Vol. 14
·期 11
·2312
·
IF 6.706
Shanshan Peng,Zhicheng Du,Yannan He,Feng Zhao,Yujing Chen,Shengchi Wu,Yuantao Hao,Li Cai
Findings on prenatal polyunsaturated fatty acids (PUFA) and offspring allergies have been inconsistent, and the majority of studies have focused on Western populations. This study aimed to investigate the associations between maternal erythrocyte PUFA and offspring allergies in the first 2 years in the Chinese population. We included 573 mother–infant pairs from a birth cohort. Based on the outpatient medical records, we identified the diagnosis and time of offspring allergic disease onset. We measured erythrocyte fatty acids by gas chromatography. Associations were examined using Cox regression. We found that higher maternal total PUFA levels (HR= 0.80; 95%CI: 0.68, 0.94), especially of arachidonic acid (AA) (HR= 0.79; 95%CI: 0.65, 0.97) andn-3 PUFA (HR= 0.77; 95%CI: 0.62, 0.97), were associated with reduced risk of offspring allergies. Similar results were found for eczema. Compared with children without a maternal allergy history, the associations of total PUFA (p= 0.028) andn-6 PUFA (p= 0.013) with offspring allergies were stronger in those with a maternal allergy history. Maternal erythrocyte total PUFA, especially AA, andn-3 PUFA were inversely associated with offspring allergies within 2 years of age. There was a significant interaction between maternal allergy history and maternal PUFA in offspring allergies.Keywords:allergic disease;polyunsaturated fatty acids;offspring;pregnancy
研究领域:
营养学
·
流行病学
·
儿科学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL IMMUNOPHARMACOLOGY
·
2025
·
IF 4.7
Longwei Yang,Wenhao Ge,Xi Lin,Ning Yu,Xiaodong Xu,Jianfa Zhang
Allergic asthma is an inflammatory airway disease in which mast cells play a key role in its pathogenesis. Riclinoctaose (Rios), an octasaccharide composed of glucose and galactose, has been reported to modulate macrophage polarization in renal ischemia-reperfusion injury. This study investigates the effects of nebulized Rios in an ovalbumin (OVA)-induced allergic asthma model. Nebulized Rios significantly reduced airway hyperresponsiveness (AHR), inflammatory cell infiltration, pulmonary fibrosis, and mucus production in OVA-treated mice. Additionally, Rios suppressed lung inflammatory cytokine production and protected against OVA-induced oxidative injury. In bronchoalveolar lavage fluid (BALF), Rios decreased the number of neutrophils and macrophages, as well as serum immunoglobulin E (IgE) and interleukin-4 (IL-4) levels. Metabolomics analysis using 1 H NMR showed significant changes in the lung metabolic profile of OVA-induced asthma mice, which were partially reversed by Rios treatment. Confocal laser microscopy (CLM) revealed that fluorescently labeled Rios specifically binds to mast cell membranes. In vitro, Rios reduced degranulation and inflammatory responses in mast cells RBL-2H3 following anti-Dinitrophenyl-Immunoglobulin E (anti-DNP-IgE) sensitization and anti-Dinitrophenyl-Bovine Serum Albumin (anti-DNP-BSA) stimulation. These results suggest that Rios has therapeutic potential for allergic asthma, likely through the modulation of mast cell degranulation and mediator release.
研究领域:
免疫学
·
呼吸病学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
IMMUNOLOGY LETTERS
·
2025
·
IF 2.8
Rui Kong,Jun Bai,Qing Yao,Xiaoqing Xu
Allergic asthma is characterized by persistent chronic airway inflammation, leading to mucus hypersecretion and airway hyperresponsiveness. Dual-specificity phosphatase 14 (DUSP14), a member of the DUSP family, is a key regulator in various biological processes. However, the function of DUSP14 in allergic asthma remains to be elucidated. In this study, we aim to explore the function and mechanism of DUSP14 in asthma-related airway inflammation. In an ovalbumin (OVA) asthma mouse model, DUSP14 was found to be significantly diminished. DUSP14 overexpression relieved airway inflammation and attenuated airway mucus production. In vitro , overexpression of DUSP14 attenuated IL-13-induced cellular inflammation and mucus hypersecretion in bronchial epithelial cells (BEAS-2B). Afterwards, we used the co-immunoprecipitation assay to confirm that DUSP14 interacted with TAK1. DUSP14 overexpression restrained the activation of TAK1 and NF-κB signaling pathway in vitro and in vivo . Taken together, our findings clearly showed that DUSP14 could alleviate airway inflammation by inhibiting TAK1 activity and NF-κB signaling pathway, positioning the DUSP14-TAK1-NF-κB regulatory axis as a potential therapeutic target for allergic asthma.
研究领域:
免疫学
·
呼吸病学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INFLAMMATION
·
2025
·Vol. 49
·期 1
·13
·
IF 5
Xiaoxu Ding,Hui Su,Tiancong Liu,Yu Chen,Zhao Gao,Ziwen Chang,Weiliang Bai
Allergic rhinitis (AR) is an inflammatory disease of the upper airway that primarily affects the nasal mucosa, with Th2 differentiation-driven inflammation as a key contributor. A bioinformatics analysis of dataset GSE52804 identified Six-transmembrane epithelial antigen of prostate 4 (STEAP4), a metalloreductase involved in inflammation regulation, as associated with AR progression, though its specific function remains unclear. Data obtained from nasal mucosal tissues from AR patients ( n = 13) and an ovalbumin (OVA)-induced AR mouse model demonstrated a marked upregulation of STEAP4. Subsequent loss-of-function experiments revealed that STEAP4 knockdown reduced Th1/Th2 imbalance-mediated inflammation, alleviating allergic symptoms in OVA-treated mice. Further investigations involved the purification of naïve CD4 + T cells from healthy murine splenocytes and their Th2 polarization. Consistently, STEAP4 knockdown inhibited Th2 differentiation and the production of Th2-related cytokines in vitro. Additionally, Guanine adenine thymine adenine sequence-binding protein 3 (GATA3), a transcription factor essential for Th2 differentiation, was predicted to bind to the STEAP4 promoter. CHIP-PCR and dual-luciferase assays confirmed the transcriptional regulation of STEAP4 by GATA3. More importantly, STEAP4 knockdown rescued the promoting effects of GATA3 overexpression on Th2 differentiation. In conclusion, STEAP4 functions downstream of GATA3 to promote AR development by promoting Th2 differentiation-mediated inflammation, suggesting its potential as a target for AR treatment.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Nanomedicine
·
2024
·
IF 6.6
Zhongxu Jing,Wenqing Li,Wei Liao,Ying Lv,Yuwei Liu,Haibo Jiang,Yufei Feng
Purpose The aim of the present study was to fabricate a Fructus Xanthii and Magnolia liliiflora volatile oils liposomes-loaded thermosensitive in situ gel (gel/LIP/volatile oil) for effectively treating allergic rhinitis via intranasal administration.Patients and Methods Particle size, polymer dispersity index (PDI), entrapment effectiveness, and cumulative drug permeation of the developed liposomes were assessed. Then, a thermoreversible in situ gel was created using the liposomes loaded with volatile oils of Fructus Xanthii and Magnolia liliiflora. The effectiveness of this treatment for allergic rhinitis was confirmed by evaluating nasal symptoms, and hematological results, after injecting the formulation into the ovalbumin (OVA)-sensitized mice, we conducted hematoxylin-eosin staining (HE) and immunohistochemistry to evaluate the outcomes. The effects of the gel/LIP/volatile oil formulation for nasal delivery of volatile oil in the treatment of rhinitis were then assessed.Results The average particle size was 95.1 ± 3.6 nm, and the encapsulation efficiencies of Fructus Xanthii and Magnolia liliiflora volatile oils were 70.42 ± 5.41% and 67.10 ± 6.08%, respectively. Drug loadings of Fructus Xanthii and Magnolia liliiflora volatile oils were 9.10 ± 0.98% and 16.10 ± 1.03%, respectively. The binary formulation produced a gel rapidly in the nasal cavity with a strong mucosal adherence at a temperature of delivering volatile oil to the nasal mucosa steadily and continuously. After nasal administration, the gel/LIP/volatile oil sustained the volatile oil delivery into the mucosa. In comparison to the monolithic formulations, the gel/LIP/volatile oil binary formulation exhibited superior performance in terms of drug delivery capability and pharmacodynamic effects.Conclusion This binary preparation displayed the ability to deliver drugs to the nasal mucosa and exhibited positive pharmacodynamic effects in treating OVA-induced rhinitis in mice. As a result, it has the potential to serve as a delivery platform for Traditional Chinese medicine in the treatment of allergic rhinitis.
研究领域:
药剂学
·
免疫学
·
中医药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Nutrition
·
2022
·
IF 6.59
Yajia Li,Qiangxiang Li,Ziqin Cao,Jianhuang Wu
Objectives: Previous studies have reported a potential association of polyunsaturated fatty acids (PUFAs) levels with allergic disease risk and the possible benefit of PUFAs supplementation on allergic disease prevention. This study was performed to estimate the genetic association between PUFAs and allergic diseases using the method of both univariable and multivariable two-sample Mendelian randomization (MR).Methods: As indicators of the PUFAs levels, we included the omega-3, omega-6, docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), linoleic acid (LA), and the ratio of omega-6 to omega-3 (omega-6:3). Summarized statistics of genome-wide association studies (GWASs) for these PUFAs were obtained from the United Kingdom Biobank and the Twins United Kingdom cohort. Genetic data relating to allergic diseases, including atopic dermatitis (AD), allergic rhinitis (AR), allergic conjunctivitis (AC), allergic urticaria (AU) and asthma, were accessed from the FinnGen biobank analysis. Odds ratios and 95% CIs were used to express the impact.Results: The MR results denoted a genetic association between the genetically determined increase in omega-3 levels and the decreased risk of some allergic diseases including AD (OR: 0.863; 95% CI: 0.785 to 0.949; p = 3.86E-03), AC (OR:0.720; 95% CI: 0.547 to 0.947; p = 1.87E-02) and AU (OR:0.821; 95% CI: 0.684 to 0.985; p = 3.42E-02), while omega-6 and DHA level was only found to have negatively correlation with risk of AC with ORs of 0.655 (95% CI: 0.445 to 0.964; p = 3.18E-02) and 0.671 (95% CI 0.490 to 0.918; p = 1.25E-02), respectively. Omega-6:3 were causally significantly associated with the increased risk of AD (OR:1.171; 95% CI: 1.045 to 1.312; p = 6.46E-03) and AC (IVW: OR:1.341; 95% CI: 1.032 to 1.743; p = 2.83E-02). After adjustment of age, economic level, BMI, smoking and alcohol behaviors in the multivariable MR analysis, a direct causal protective effect of omega-3 on AD and AC, as well as a direct causal association between DHA and AD were observed. Omega-6:3 was also found to be directly associated with an increased risk of AD and AC. No association was found of EPA or LA with allergic diseases.Conclusion: Higher PUFA concentrations (omega-3, omega-6, DHA) and lower omega-6:3 ratios were genetically associated with a lower risk of some allergic diseases.
研究领域:
免疫学
·
营养流行病学
·
遗传流行病学
·
过敏反应
·
公共卫生
✓ 已验证
DOI
PubMed
高影响因子
PLoS One
·
2012
·Vol. 7
·期 5
·e37654
·
IF 4.092
Mingjia Chen,Hongmei Zeng,Dewen Qiu,Lihua Guo,Xiufen Yang,Huaixing Shi,Tingting Zhou,Jing Zhao
Background
Magnaporthe oryzae , the rice blast fungus, might secrete certain proteins related to plant-fungal pathogen interactions.
Methodology/Principal Findings
In this study, we report the purification, characterization, and gene cloning of a novel hypersensitive response-inducing protein elicitor (MoHrip1) secreted by M. oryzae . The protein fraction was purified and identified by de novo sequencing, and the sequence matched the genomic sequence of a putative protein from M. oryzae strain 70-15 (GenBank accession No. XP_366602.1). The elicitor-encoding gene mohrip1 was isolated; it consisted of a 429 bp cDNA, which encodes a polypeptide of 142 amino acids with a molecular weight of 14.322 kDa and a pI of 4.53. The deduced protein, MoHrip1, was expressed in E. coli . And the expression protein collected from bacterium also forms necrotic lesions in tobacco. MoHrip1 could induce the early events of the defense response, including hydrogen peroxide production, callose deposition, and alkalization of the extracellular medium, in tobacco. Moreover, MoHrip1-treated rice seedlings possessed significantly enhanced systemic resistance to M. oryzae compared to the control seedlings. The real-time PCR results indicated that the expression of some pathogenesis-related genes and genes involved in signal transduction could also be induced by MoHrip1.
Conclusion/Significance
The results demonstrate that MoHrip1 triggers defense responses in rice and could be used for controlling rice blast disease.
研究领域:
植物病理学
·
分子生物学
·
农学
✓ 已验证
DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Jia-Hui Ma,Li-Qiu Ren,Tian-Xuan Tang,Yu-Yao Chen,Chen-Xi Zhang,Yu-Feng Ke,Yan Zhang,Mitchell N. Muskat,Xiang-Rong Cheng
This study investigated the effects of polyphenols from walnut pellicle on the structure and allergenicity of walnut globulin (WG). The allergenicity-guided assay revealed walnut globulin (WG) from walnut defatted powder without pellicle was the most allergenic protein fraction of walnut. Complexes of phenolic extracts from walnut pellicle (PEWP) and WG were prepared in different ratio and pH conditions to further investigate the effect of PEWP on the protein structure and allergenicity of globulin. The covalent interaction between PEWP and WG in pH 7 was lower than pH 11, according to the free amino groups' content and SDS-PAGE analysis. Comprehensive methods characterized the complexes, including UV–Visible, fluorescence, Fourier transform infrared, and circular dichroism spectroscopy, and indicated that combination of polyphenols with protein altered the secondary and tertiary structure of the protein, leading to the unfolding of the protein structure. Meanwhile, the combination with PEWP reduced the IgG-binding capacity of WG in a dose-dependent manner, reaching a minimum of 26.90 ± 4.31% (at pH 7), which was significantly (P < 0.05) lower compared to that at pH 11 (41.79 ± 1.25%). Correlation analysis revealed a significant correlation between IgG-binding capacity and protein structure, as well as the addition concentration of PEWP (r = −0.85, P < 0.01), and pH conditions may indirectly influence the structure and allergenicity of the protein by affecting the interaction between WG and PEWP. The interaction between WG and PEWP was characterized through fluorescence-quenching mechanism analysis, indicating that polyphenols from PEWP mainly interact with WG through hydrogen bond and hydrophobic interaction.
研究领域:
食品科学
·
免疫学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
Advanced Healthcare Materials
·
2023
·Vol. 12
·期 29
·2302059
·
IF 10
Shengnan Liu,Qianru Yu,Rui Guo,Kuntao Chen,Jiao Xia,Zhenhu Guo,Lu He,Qian Wu,Lan Liu,Yunxuan Li,Bozhen Zhang,Lin Lu,Xing Sheng,Jiahua Zhu,Lingyun Zhao,Hui Qi,Ke Liu,Lan Yin
Bioadhesive hydrogels have attracted considerable attention as innovative materials in medical interventions and human–machine interface engineering. Despite significant advances in their application, it remains critical to develop adhesive hydrogels that meet the requirements for biocompatibility, biodegradability, long-term strong adhesion, and efficient drug delivery vehicles in moist conditions. A biocompatible, biodegradable, soft, and stretchable hydrogel made from a combination of a biopolymer (unmodified natural gelatin) and stretchable biodegradable poly(ethylene glycol) diacrylate is proposed to achieve durable and tough adhesion and explore its use for convenient and effective intranasal hemostasis and drug administration. Desirable hemostasis efficacy and enhanced therapeutic outcomes for allergic rhinitis are accomplished. Biodegradation enables the spontaneous removal of materials without causing secondary damage and minimizes medical waste. Preliminary trials on human subjects provide an essential foundation for practical applications. This work elucidates material strategies for biodegradable adhesive hydrogels, which are critical to achieving robust material interfaces and advanced drug delivery platforms for novel clinical treatments.
研究领域:
材料科学
·
医学
·
药学
✓ 已验证
DOI
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Wenjun Xue,Xiaoyu Kuang,Xuanzhen Meng,Boyu Sun,Zijun Zhao,Guizhao Liang
Given the rising incidence rate of chronic allergic diseases and the side effects of traditional anti-allergic drugs, this study aimed to identify novel hyaluronidase inhibitory peptides from various food-derived collagen by an integrated computational screening strategy with in vitro inhibitory activity verification, and to elucidate the inhibition mechanism of peptides based on the simulated dynamic characteristics, quantum chemistry calculation, and weak interaction analysis. Two potent hyaluronidase inhibitory peptides with non-toxicity, non-allergenicity, good water solubility, and good simulate gastrointestinal stability, that is, Pro-Gly-Pro-Ala-Gly-Arg (PGPAGR) from Nile tilapia and Gly-Pro-Ser-Gly-Pro-Arg (GPSGPR) from rainbow trout, Nile tilapia, donkey, bovine, and domestic pig, were identified with IC 50 values of 11.98 ± 0.42 mM, 13.64 ± 0.21 mM, respectively. During the 100 ns dynamic interaction, two peptide-hyaluronidase complex systems both remained relatively stable. Additionally, the dynamic binding of these two peptides further enhanced the structural stability and compactness of hyaluronidase, and reduced the inverse movement between different residues of hyaluronidase. The results of residue free energy decomposition showed that residues Tyr75, Tyr202, Trp321, and Trp324 made prominent positive contributions to the binding of peptide GPSGPR, while residues Tyr202, Tyr247, and Arg265 made prominent positive contributions to the binding of peptide PGPAGR. The results of weak interaction analysis showed that hydrogen bond and van der Waals interactions were the dominant weak interaction types in the dynamic binding between the peptides and hyaluronidase. This study will provide theoretical basis and data support for the development of novel anti-allergic functional foods and drugs, and offer novel strategies for the prevention and improvement of allergic diseases.
研究领域:
生物化学
·
药理学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2025
·
IF 3.5
Guangliang Xing,Jia Liu,Ruyi Tang,Tianran Hui
BACKGROUND The escalating global prevalence of food allergies has intensified the need for hypoallergenic food products. Transglutaminase (TGase)-mediated crosslinking has garnered significant attention for its potential to reduce the allergenicity of food proteins. This study aimed to investigate the effects of TGase crosslinking on the potential allergenicity and conformational changes in a dual-protein system composed of β -lactoglobulin ( β -LG) and soy protein isolate (SPI) at varying mass ratios (10:0, 7:3, 5:5, 3:7 and 0:10 (w/w)). RESULTS TGase preferentially crosslinked the 7S and 11S subunits of soy protein, rather than β -LG. Crosslinking treatment reduced the allergenic potential of both soy protein and β -LG, with the degree of reduction depending on the protein ratio. The β -LG5:SPI5 and β -LG7:SPI3 mixtures showed the most significant reduction in antibody reactivity towards soy protein and β -LG, respectively. Additionally, TGase-mediated crosslinking significantly reduced the binding capacity of all dual-protein samples to serum immunoglobulin E from allergic patients, compared to the control group ( P < 0.05). The allergenicity reduction was accompanied by structural modifications, including a decrease in β -sheet content, an increase in β -turn and random coil structures, enhanced ultraviolet absorption and intrinsic fluorescence, reduced free sulfhydryl levels and altered intermolecular forces. These changes suggest that TGase-induced crosslinking may disrupt or mask allergenic epitopes, thus lowering allergenicity. CONCLUSION TGase crosslinking effectively reduced the allergenic potential of the dual-protein system by inducing structural changes. These findings underscore the potential of TGase in developing hypoallergenic dual-protein food products and provide a scientific foundation for future research and application in the food industry. © 2025 Society of Chemical Industry.
研究领域:
食品科学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）