CATALYSIS LETTERS
·
2021
·Vol. 151
·期 9
·2537-2546
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IF 3.186
Zhang Qianqian,Zheng Shanshan,Zhang Jinli,Li Wei,Fu Yan
NADH, composed of metal binding, charged and redox groups, acts as a “three-in-one” template to construct peroxidase mimicking Pd nanoclusters (NCs). The physicochemical properties of Pd NCs are highly dependent upon the molar ratio of [NADH]/[Na 2 PdCl 4 ], thereby contributing to distinct peroxidase mimicking activities. The ultrasmall Pd nanozyme that contains 30% metallic Pd 0 species, exhibits the K m of 0.063 mM toward TMB and 80.8 mM toward H 2 O 2 . Notably, addition of heparin enables the enhancement of its peroxidase-like activity in neutral media. A colorimetric assay was well-established at pH 6 for quantitatively monitoring heparin in aqueous solution and biological fluid. The linear response lies in the range of 0.5–25 µg mL −1 , with the limit of detection of 1.1 ng mL −1 . This work paves a promising pathway to manufacture highly active enzyme mimetics with desirable physicochemical properties. Graphic Abstract
研究领域:
生物化学
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纳米医学
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分析化学
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DOI
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 3
·1-8
·
IF 5.479
Zhong Qingmei,Chen Yuye,Qin Xiu,Wang Yilin,Yuan Chunling,Xu Yuanjin
Carbon quantum dots (CQDs) with peroxidase-mimicking activity were successfully prepared from litchi rind. A colorimetric method for glucose determination was developed based on etching of gold nanorods (GNRs) using CQDs as peroxidase mimetic. The glucose oxidase-catalyzed oxidation of glucose leads to the generation of H 2 O 2 which oxidizes added iodide under formation of elemental iodine under the catalytic action of CQDs. Iodine then etches the GNRs along the longitudinal direction due to the higher reaction activities at the tips of GNRs. This results in a stepwise decrease in the maximum absorption wavelength of the GNRs, from initially 953 nm to finally 645 nm. Under the optimized conditions, the shift in the maximum absorption wavelength decreases linearly in the 0.01–2.0 mM glucose concentration range, and the detection limit is 3.0 μM. Importantly, this method was applied to the determination of glucose in human serum. It is perceived that the CQDs are valuable peroxidase mimics due to their ease of preparation, low costs and stable catalytic activity.
Carbon quantum dots were prepared from litchi rind. They can induce the oxidation of gold nanorods in the presence of I − ions and H 2 O 2 . This finding was applied to design a colorimetric assay for glucose.
研究领域:
分析化学
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生物医学工程
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DOI
PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 71
·66963-66970
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IF 3.289
Haiguan Yang,Junqi Zha,Peng Zhang,Yuhao Xiong,Linjing Su,Fanggui Ye
CoS, which was prepared using a facile solvothermal method, and characterized using various analytical techniques, was demonstrated for the first time to exhibit intrinsic peroxidase-like activity. CoS catalyzed the oxidation of the peroxidase substrate 3,3′,5,5′-tetramethylbenzidine in the presence of H2O2 rendering the reaction solution blue, which is the principle used for H2O2 sensing. The mechanism of the CoS catalysis was also investigated. Under optimal conditions, the linearity of H2O2 detection ranged from 0.05 to 0.8 mM (R2 = 0.9965). More importantly, using the reducibility of glutathione and the strong affinity to thiol compounds exhibited by mercury ions (Hg2+), a novel “off–on” colorimetric sensor for Hg2+ determination was designed using CoS as a peroxidase mimics. The analytical platform for Hg2+ determination was developed, ranging from 0.25 to 3 μM (R2 = 0.9965). The limit of detection was 0.1 μM. CoS exhibited several advantageous features including good chemical stability, ease of preparation, simple purification, and excellent reproducibility.
研究领域:
生物化学
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分析化学
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材料科学
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DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
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2025
·
IF 19
Gufeng Liu,Xiaoyi Wu,Lei Tan,Tiange Cheng,Chengyu Yang,Xin Liu,Fengxiao He,Ziqiao Tang,Qi Yan,Liangliang Fu,Min Wang,Haibin Xia,Tao Wu
Peri-implantitis is an inflammatory disease characterized by progressive bone loss, challenging implant longevity and incurring significant treatment burden. Current reconstruction therapy involves mechanical scaling followed by artificial bone grafting. However, residual bacteria and inflammation memory often lead to recurrence, highlighting the critical need for the artificial bone substitute with sustained anti-inflammatory and antibacterial properties. By utilizing the phosphate groups of mesoporous hydroxyapatites for in situ loading of CeO 2 , followed by a reduction method to fabricate a CeO 2 -Cu heterojunction interface, this study develops Bonyzymes with anti-inflammatory, antibacterial, and osteogenic properties. The incorporation of Cu modulates the electronic environment of the CeO 2 surface, increasing superoxide dismutase and catalase activities of Bonyzymes. In vitro, the Bonyzymes effectively regulated oxidative stress by scavenging excess reactive oxygen species, exerting anti-inflammatory effects. Additionally, it exhibits antibacterial activity against Staphylococcus aureus , Escherichia coli and Porphyromonas gingivalis , and facilitates osteogenic differentiation in bone marrow stem cells. In vivo experiments in rat peri-implantitis models confirm its ability to promote bone regeneration in the infected peri-implant area. Overall, this work develops novel artificial bone substitutes, Bonyzymes, which not only promotes bone regeneration but also exhibits anti-inflammatory and antibacterial properties, offering a promising strategy for peri-implantitis treatment.
研究领域:
生物材料
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口腔医学
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纳米技术
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炎症调控
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DOI
高影响因子
MICROCHIMICA ACTA
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2024
·Vol. 191
·期 7
·1-12
·
IF 5.3
Zhou Peipei,Hong Yangluchen,Fan Ru,Wang Huili,Wang Xuedong,Mei He
To realize the reutilization of waste Myrica rubra in the analytical field, we synthesized Myrica rubra –based N-doped carbon dots (MN-CDs) and further anchored them onto the surface of Fe 3 S 4 to fabricate Fe 3 S 4 @MN-CD nanocomposites. The as-fabricated nanocomposites possessed higher peroxidase-mimetic activity than its two precursors, resulting from the synergistic effect between them, and could catalyze colorless 3,3′,5,5′-tetramethylbenzidine (TMB) into deep blue oxTMB with a strong 652-nm absorption. Under optimized conditions (initial solution pH, 3.5; incubation temperature, 35 ℃; Fe 3 S 4 @MN-CD concentration, 50 µg mL −1 , and 652-nm absorption), Fe 3 S 4 @MN-CDs were employed for colorimetric assay of p -aminophenol ( p -AP) with wide linear range (LR, 2.9–100 µM), low detection limit (LOD, 0.87 µM), and satisfactory recoveries (86.3–105%) in environmental waters. Encouragingly, this colorimetric assay provided the relative accuracy of 97.0–99.4% as compared with conventional HPLC-UV detection. A portable smartphone-based colorimetric application was developed by combining the Fe 3 S 4 @MN-CD-based visually chromogenic reaction with a “Thing Identify” APP software. Besides, we engineered an image-capturing device feasible for field use, in which the internal-compact sealing prevented external light source from entering photography chamber, thereby reducing light interference, and also the bottom light source enhanced the intensity of blue imaging. This colorimetric platform exhibited satisfactory LR (1–500 µM), low LOD (0.3 µM), and fortification recoveries (86.6–99.6%). In the chromogenic reaction catalyzed by Fe 3 S 4 @MN-CDs, ·O 2 − played a key role in concomitant with the participation of •OH and h + . Both the colorimetric assay and smartphone-based intelligent sensing show great promising in on-site monitoring of p -AP under field conditions. Graphical Abstract
研究领域:
分析化学
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环境科学
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材料科学
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2020
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IF 3.232
Qiulan Li,Dezhi Yang,Yaling Yang
The study presents a spectrofluorimetric method for the determination of Cr(III) based on 2,3-diaminophenazine (DAP) as fluorescent nano sensor. With the peroxidase-mimicking activity of copper-doped carbon dots (Cu-CDs), colorless o -phenylenediamine (OPD) was oxidized to fluorescent DAP in the presence of H 2 O 2 via generation of hydroxyl radicals . The Cr(III) was found to decrease the fluorescent intensity of the Cu-CDs-mediated OPD oxidation system. Cr(VI) species were reduced to Cr(III) by employing 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonate) (ABTS) as the reductant, and a novel spectrofluorimetric method for the highly sensitive detection of speciation Cr(VI) and Cr(III) was developed. The Cu-CDs nanozyme was prepared from ethylenediaminetetraacetic acid copper disodium salt (Na 2 [Cu (EDTA)]) by one-step pyrolysis method. Under optimal conditions, the fluorescence intensity of DAP is linearly proportional to the Cr(III) ion concentration in the range of 5 × 10 −6 to 1.5 × 10 −4 mol L −1 and the detection limit is 1.2 × 10 −7 mol L −1 . The method has a good anti-interference performance against some metal ions , such as, Hg 2+ , Co 2+ , Cu 2+ , Mg 2+ , Ba 2+ , K + , Mn 2+ , Ni 2+ , Al 3+ , and Fe 3+ . The proposed method was successfully applied in the determination of Cr(VI) and Cr(III) in water samples.
研究领域:
分析化学
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环境科学
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DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
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2022
·Vol. 46
·期 25
·12372-12380
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IF 3.925
Juan Chai,Luohao Yuan,Shiwei Wang,Tong Li,Mingxue Wu,Zhiwei Huang,Hongfeng Yin
Three metal–organic frameworks {[Cu6(L1)4(DMF)(H2O)2·5H2O]}n (1), {Cu3(L1)2(phen)2(H2O)}n (2) and {[Cu8(μ3-OH)3(L2)4(NO3)(H2O)5]·3H2O}n (3) utilizing nitrogen-containing heterocyclic carboxylate bifunctional ligands (L1 = 5-(4-carboxypyridin-3-yl)isophthalic acid and L2 = 2-(5-carboxypyridin-2-yl)terephthalic acid) were self-assembled hydrothermally and characterized. MOFs 1–3 possess 3D networks which consist of three different types of Cu clusters {Cu2O2(OCO)4}, {Cu2O2N4(OCO)2} and {Cu4O10(OCO)4}, respectively. MOFs 1–3 efficiently catalyze the oxidation of cycloalkanes under mild conditions. Besides, the synthesized MOFs 1–3 exhibited high peroxidase-like activity and could be utilized to catalyze the oxidation of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid ammonium salt) (ABTS) to produce green color in the presence of hydrogen peroxide (H2O2). The steady-state kinetics experiments of the oxidation of ABTS catalyzed by MOFs 1–3 were carried out, and the kinetic parameters were obtained. The results indicated that the affinity of MOFs 1–3 towards H2O2 and ABTS was higher than that of the natural horseradish peroxidase (HRP). The as-prepared MOF 1 can be applied for the colorimetric detection of H2O2 with a wide linear range of 0.5 to 200 μM and a limit of detection (LOD) of 0.097 μM. Therefore, the novel Cu-MOFs could be regarded as promising artificial enzyme mimics in catalysis, sensors and environment analysis.
研究领域:
材料科学
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生物化学
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分析化学
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DOI
高影响因子
ACS Sustainable Chemistry & Engineering
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2021
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IF 8.198
Xuefang Zheng,Qi Lian,Liya Zhou,Yanjun Jiang,Jing Gao
A simple and efficient strategy was developed to synthesize polyacrylonitrile-copper oxide (PAN-CuO) nanoflowers by growing copper oxide nanoneedles on the surface of polyacrylonitrile (PAN) nanoflowers under mild conditions. Fortunately, the obtained PAN-CuO nanoflowers exhibit excellent peroxidase-mimicking activity to catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to form a blue-colored solution with H2O2. The peroxidase-like mechanism originated from the generation of the •OH radical. The PAN-CuO-based colorimetric assay was used to detect H2O2 with a detection limit (LOD) of 0.12 μM in a linear range from 0.5 to 125 μM. The colorimetric method was simple, selective, and sensitive for ascorbic acid (AA) detection in the range of 1–180 μM with a detection limit (LOD) of 0.56 μM. Moreover, the proposed PAN-CuO-based colorimetric assay could be used in the real sample analysis. The peroxidase-like PAN-CuO nanoflowers could provide a versatile catalytic platform for the application of the colorimetric assay method and biomimetic enzyme catalytic systems, and open a new way for detection of AA in biological systems.
研究领域:
材料科学
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生物化学
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分析化学
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DOI
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
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2024
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IF 4.1
Samaneh Rashtbari,Gholamreza Dehghan,Yasin Orooji,Simin Khataee,Arezu Marefat,Leonid G. Voskressensky,Alireza Khataee
The extensive use of pesticides in modern agriculture has raised concerns about environmental contamination and adverse human health effects. Therefore, developing highly sensitive detection methods to identify pesticide residues is crucial for food safety and ecosystem protection. In this study, the Fe 2 AlB 2 MAX phase was synthesized and characterized. After evaluating its peroxidase-mimetic performance using a colorimetric method, a new, sensitive, and simple dual fluorescence-colorimetric sensor was developed for the quantification of two common pesticides, acetamiprid (ACP) and imidacloprid (IMP), using fluorescein (FL). The developed method is based on the inhibitory impact of ACP and IMP on the enzymatic performance of the Fe 2 AlB 2 MAX phase, specifically the inhibition of hydroxyl radical ( OH) generation, which enhances the absorption and emission intensities of FL. The results confirmed that OH generated through the breakdown of H 2 O 2 via the catalytic activity of the MAX phase can decrease the intrinsic absorption and emission intensities of FL. However, ACP and IMP inhibit the peroxidase-like activity of the MAX phase, leading to increased absorption and emission intensities of FL. The limit of detection values calculated for spectrophotometric and spectrofluorimetric quantifications were 2.8 μM and 0.051 μM for ACP and 1.72 μM and 0.013 μM for IMP, respectively. Furthermore, the proposed method was successfully utilized to accurately and reliably determine ACP and IMP in spiked real samples with satisfactory accuracy and precision. These developed methods offer several advantages, making them promising candidates for the direct, rapid screening of pesticide residues.
研究领域:
食品安全
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环境科学
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分析化学
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产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Ya Xu,Jihong Zhou,Peng Sun,Ping Li,Dongxue Han,Xiaohong Wang,Fang Chai
Artificial mimic enzymes have attracted wide attention for their superior characteristics to natural enzymes in extensive applications of diagnosis and therapy. In this paper, a nanozyme Cu-BDC@FeMo 6 was designed and fabricated by embedding polyoxometalate (NH 4 ) 3 [FeMo 6 O 18 (OH) 6 ]·6H 2 O (FeMo 6 ) into a copper-based metal–organic framework (Cu-BDC). The integration of FeMo 6 and Cu MOF based on the synergies of oxidability of FeMo 6 and oxygen-driven reversible Cu + /Cu 2+ enhanced the peroxidase mimicking activity, which accomplished the sensitive visual monitoring of H 2 O 2 and dopamine (DA). The detection of H 2 O 2 was driven by Cu-BDC@FeMo 6 catalyzing the oxidation of TMB with colorimetric evolution to blue, and consecutive monitoring DA via the reverse process of reduction of ox-TMB was further achieved. The limit of detection (LOD) of H 2 O 2 and DA were 10 μM and 2.27 μM, with excellent stability, and outstanding selectivity. The mechanism of the catalysis was further evaluated, and the generation of O 2 · - played a crucial role in the catalysis for oxidation. The logic gate design was constructed to illustrate the process and application. This work provides a feasible reference for the reasonable design of simulated enzyme in biosensor applications.
研究领域:
生物传感
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材料科学
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仿生催化
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产品关联
DOI
高影响因子
DALTON TRANSACTIONS
·
2019
·Vol. 48
·期 6
·1993-1999
·
IF 4.052
Shichao Lin,Yihong Zhang,Wen Cao,Xiaoyu Wang,Li Qin,Min Zhou,Hui Wei
Carbon nanozymes are catalytic carbon nanomaterials with intrinsic enzyme-like activities. They are advantageous over their natural counterparts in terms of higher stability, lower preparation cost, and better robustness. However, the peroxidase-like activities of the most developed carbon nanozymes were moderate due to the imperfection of active centers and limited tuning strategies. Herein, we designed a novel class of efficient peroxidase-mimicking carbon nanozymes with nitrogen atom doping. The N-doped carbon nanozymes were facilely synthesized by direct pyrolysis of different nucleobases at controlled temperatures without other treatments. A high ratio of nitrogen atoms was doped into the carbon skeleton. For example, 8.77 wt% of N remained in the guanine-derived carbon nanozyme with a pyrolysis temperature of 900 °C. The dominant graphitic N species greatly boosted the peroxidase-like activities of nucleobase-derived carbon nanozymes. Moreover, nucleobases are cheap, abundant, and environmentally friendly. We have demonstrated that nitrogen-rich nucleobases are ideal starting materials for the large-scale and cost-effective synthesis of N-doped carbon nanozymes. The carefully designed N-doped carbon nanozymes with superior activities were further used to construct effective biosensors for bioactive molecules (i.e., H2O2 and glucose). Highly sensitive and selective detection of H2O2 and glucose was achieved using the N-doped carbon nanozymes as efficient peroxidase mimics. This study offers an economical and sustainable approach for the scalable preparation of N-doped carbon nanozymes and creates a new path for the rational design of efficient peroxidase-mimicking carbon nanozymes by heteroatom doping.
研究领域:
生物化学
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材料科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
STARCH-STARKE
·
2017
·
IF 1.837
Linjing Su,Yuhao Xiong,Mei Wu,Zhenhua Duan,Zhi Zhang,Wei Xie,Dongjian Zhu,Yanghe Luo,Xingcun He
Researchers remain attracted to the development of more efficient and cost-effective materials for the degradation of organic dyes in wastewater. Ultra-small (sub-5 nm) Fe 2 O 3 nanocrystals embedding magnetic porous carbon, referred to as Fe 2 O 3 @C, in which starch is used as a carbon source, is demonstrated, and is synthesized by a facile microwave-enhanced high-temperature ionothermal method within 4 min. The Fe 2 O 3 @C obtained is used as a peroxidase-mimicking catalyst in heterogeneous Fenton-like reactions to degrade organic dyes (i.e., methylene blue) with aid from the green oxidant, hydrogen peroxide. Because of its unique structure and high specific surface area, Fe 2 O 3 @C displays enhanced treatment properties in the degradation of methylene blue. Moreover, Fe 2 O 3 @C demonstrates stability and resistance to a broad range of pH, recyclability, and convenient separation. This promising peroxidase-mimicking composite material may have potential applications in degradation of organic pollutants in the environmental cleaning field.
研究领域:
环境科学
·
材料科学
·
生物催化
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）