RSC Advances
·
2014
·Vol. 4
·期 33
·17387-17392
·
IF 3.708
Wengfeng Zhu,Jian Zhang,Zhenchao Jiang,Weiwei Wang,Xiaoheng Liu
In this paper, bright and high-quality carbon dots (CDs) were prepared by refluxing a membranous carbonized β-cyclodextrin in nitric acid. The structure and optical properties of the CDs were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR), fluorescence spectrophotometry (FL), X-ray diffraction (XRD) and Raman spectroscopy. The mechanism for the formation of the CDs is also discussed. As-prepared CDs could be used as peroxidase mimics for the detection of H2O2. The results indicate that the CDs are capable of rapid and sensitive detection of H2O2 in the linear range from 2 × 10−6 to 5 × 10−4 mol L−1 and the limit of detection of H2O2 is as low as 1 × 10−6 mol L−1. More importantly, the peroxidase-like activity of the CDs is expanded to the detection of some oxidative ions (such as Fe3+ and Ag+). The linear ranges for Fe3+ and Ag+ were 8 × 10−6 to 1 × 10−4 mol L−1 and 5 × 10−6 to 1 × 10−4 mol L−1 respectively, with corresponding detection limits of 8 × 10−7 mol L−1 and 5 × 10−7 mol L−1 respectively.
研究领域:
材料科学
·
分析化学
✓ 已验证
DOI
高影响因子
Journal of Material Cycles and Waste Management
·
2024
·
IF 2.7
Zhu Wenzhong,Zhu Xueting,Shen Weijian,An Nan,Wu Lina
Antibiotics are extensively employed in the treatment of human and animal ailments. However, their excessive use and poor biodegradability have led to a notable accumulation of antibiotics in the environment, presenting potential health hazards to humans. In this study, tetracycline (TC) was selected as a representative antibiotic, and a recyclable material, Mn-ZIF-67, was synthesized to degrade TC through adsorption and peroxidase-like activity. Mn-ZIF-67 significantly enhances the peroxidase-like activity of ZIF-67. The removal efficiency of TC was boosted by 300% compared to ZIF-67 within a 10-min timeframe. With its high catalytic activity and stability, Mn-ZIF-67 exhibits significant promise for the swift degradation and elimination of antibiotics from the environment.
研究领域:
环境科学
·
材料科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Yuting Zhuang,Mengqi Jian,Huiqi Yan,Xin Lv,Yue Zhang
Selenium is a nutritionally essential trace element for human health, but efficient or excessive intake both causes physiological diseases. Here, we propose that selenium as a brightening agent significantly increase the peroxidase (POD)-like activity of MXene@CeO 2 , which CeO 2 nanoparticles in-situ grow on MXene as the sensing platform. Based on the selenium-stimulated POD-like activity of MXene@CeO 2 , a simple, low-cost, and reliable colorimetric method is constructed for quantitative analysis of selenium. The great advantage of MXene@CeO 2 platform selecting selenium as brightening agent exhibits good selectivity for other 23 interfering ions, allowing the discrimination of selenium in practical samples rapidly and precisely even at 3.5 nM. Moreover, MXene@CeO 2 platform selectively catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) instead of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium (ABTS) under the same condition. This study not only offers a utility sensing platform for quick detection of selenium but also opens an avenue for the rational design of element sensors for point-of-care application.
研究领域:
生物化学
·
医学检测
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2017
·Vol. 142
·期 20
·3986-3992
·
IF 3.885
Hao-Hua Deng,Xiao-Qing Zheng,Yan-Yu Wu,Xiao-Qiong Shi,Xiu-Ling Lin,Xing-Hua Xia,Hua-Ping Peng,Wei Chen,Guo-Lin Hong
We herein report the intrinsic alkaline peroxidase-like activity exhibited by CuO nanoparticles when 3-(4-hydroxyphenyl)propionic acid was employed as a substrate. Based on this observation, a fluorometric assay method with a low detection limit of 0.81 μM was established for H2O2 determination under alkaline conditions. Notably, ammonia was found to inhibit the alkaline peroxidase-like activity of the CuO nanoparticles. Thus, a sensing platform for the determination of urea and urease was successfully constructed, with the limits of detection for urea and urease being 27 μM and 2.6 U L−1, respectively. This platform was then applied for the detection of urea in human urine and urease in soil, which yielded satisfactory results. These results suggest that it is possible to extend the catalytic potential of peroxidase and its mimetics from acidic and neutral conditions to include activity in alkaline media as well. Furthermore, this strategy is a novel method for the analysis of urea and urease. The assay developed in this work is facile, inexpensive, convenient, and highly selective and sensitive. Therefore, it is expected that this system can serve as a template for the development of similar enzyme nano-mimics.
研究领域:
生物化学
·
纳米生物学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
Molecular Catalysis
·
2017
·
IF 0
Li Liu,Ying Shi,Menglu Li,Chaoqun Sun,Yijuan Long,Huzhi Zheng
Recently, an important and intriguing property of fluorescein to function as an artificial peroxidase was found. Although, as a new class of small molecule enzyme mimics, the catalytic activities should be highly associated with the composition and structure, little is known about the mechanism underlying the functional groups mediated enzyme-like activities. Herein, we report for the first time that fluorescein derivatives, i.e. , carboxyfluorescein (CF) and aminofluorescein (AF) possess peroxidase-like activity, and investigate the impact of different functional groups on the catalytic performance. By systematically evaluating absorption intensity, electron spin resonance (ESR) signals, enzyme kinetic parameters and activation energies, we obtain evidence that the carboxyl group can enhance catalytic activity and may serve as substrate-binding sites, but the amino group can even inhibit the catalytic reaction. Correspondingly, the theoretical study further identified these experimental results in molecular-level. These observations pave the way for identifying highly effective functional groups that promote the overall performance of fluorescein-based artificial peroxidase. Meanwhile, this work should facilitate the design and construction of tailor-made small molecule peroxidase mimics.
研究领域:
生物化学
·
分子生物学
✓ 已验证
产品关联
DOI
Frontiers in Bioengineering and Biotechnology
·
2025
·
IF 4.8
Ye Yuan,Yuan Liu,Zhipeng Shen,Huidan Wu,Lantian Meng,Xiaoxiao Guo,Bing Jiang,Ling Fang
Background: Bacterial resistance remains a significant challenge, necessitating the development of new antibacterial strategies. This study introduces a rapidly synthesized L-histidine- Fe3O4 (L-His-Fe3O4) nanozyme with enhanced peroxidase (POD)-like activity, designed to improve antibacterial efficacy and accelerate the healing of bacteria-infected wounds.Methods: We successfully synthesized L-His-Fe3O4 using an ultrafast, room-temperature synthesis method, and observed its anti-infection effect and explored its anti-infection mechanism through in vivo and in vitro antibacterial experiments.Results: We produced L-His-Fe3O4 cost-effectively while preserving L-His, which was essential for its catalytic and antibacterial functions. The resulting nanozyme demonstrated exceptional antibacterial activity against both Gram-positive (S. aureus) and Gram-negative (E. coli) bacteria. In vivo experiments revealed that L-His-Fe3O4 outperformed vancomycin in reducing bacterial viability and effectively promoting wound healing, all while maintaining excellent biosafety with no adverse effects on blood or liver functions.Discussion: These findings highlight the potential of L-His-Fe3O4 for large-scale production and practical use in treating bacterial infections, offering a promising approach to combating antibiotic-resistant pathogens.
研究领域:
生物材料学
·
微生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2020
·Vol. 8
·期 30
·6451-6458
·
IF 5.344
Daomeng Liu,Daquan Wang,Xunan Jing,Xiaoping Zhao,Duo Xi,Dongfeng Dang,Lingjie Meng
Nanozymes are a new generation of artificial enzymes that address the limitations of natural enzymes, with numerous reports on the development of high performance nanozymes for various applications. Herein, the phase regulation of network-like MoSe2 from the metal 1T phase to semiconductor 2H phase was achieved under different reaction conditions by a facile but efficient microwave-assisted solvothermal method. The coexisting 1T/2H-MoSe2 with edge enriched and defective nanostructures showed great enhancement of the peroxidase-like properties. This high enzymatic activity may arise from optimized active sites of 1T/2H-MoSe2, effectively leading to a rapid charge transfer within the substrate. In addition, chitosan functionalization not only greatly improves the dispersion stability of MoSe2, but also significantly increases its peroxidase-like activity, probably due to its enhanced affinity to hydrogen peroxide (H2O2). Based on these results we have established a highly sensitive colorimetric assay for the detection of H2O2 and glucose in human serum.
研究领域:
材料科学
·
生物催化
·
医学检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Food Measurement and Characterization
·
2021
·Vol. 15
·期 2
·1747-1753
·
IF 2.431
Li Yi-Fang,Lin Zheng-Zhong,Hong Cheng-Yi,Huang Zhi-Yong
A facile colorimetric method for rapid detection of putrescine (Put) and cadaverine (Cad) based on the peroxidase-like activity of (Fe,Co) codoped-CDs was developed. With the enzymatic hydrolysis by diamine oxidase (EC 1.4.3.6, DAO), Biogenic amines (BAs) were decomposed to produce H 2 O 2 which oxidizes 3,3',5,5'-tetramethyl-benzidine with the catalysis of (Fe,Co)-codoped CDs. By means of principal component analysis, Put and Cad were differentiated from BAs and their total amounts were detected by measuring the spectral absorption at 652 nm with a linear range from 0.25 to 10 mg kg −1 . The colorimetric method was applied to the detection of Put and Cad in various fish samples with a detection limit ((3σ/k, n = 9)) of 0.06 mg kg −1 . Besides verifying with HPLC, the recoveries of the colorimetric method were also tested by spiking standards at three levels with the range of 98.2%–115.7%, indicating that the established colorimetric method was accurate and sensitive.
研究领域:
食品科学
·
生物化学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2016
·Vol. 183
·期 7
·2123-2129
·
IF 4.831
Li Xue-Jiao,Wang Yong-Sheng,Yang Sheng-Yuan,Tang Xian,Liu Lu,Zhou Bin,Wang Xiao-Feng,Zhu Yu-Feng,Huang Yan-Qin,He Shun-Zhen
We report on a photometric method for the determination of the metallothioneins (MTs). It is known that citrate capped gold nanoparticles (AuNPs) coated with traces of mercury possess peroxidase-like properties that can catalyze the oxidation of 2,2′-azino-bis(3-ethylbenzothiazoline- 6-sulfonate) (ABTS) to form a blue product in acetate buffer of pH 4.5. It is found that if the AuNPs are first aggregated by the cysteine-rich metallothioneins, the peroxidase-like properties of the resulting aggregates (AuNP-Hg-MTs) cause a largely accelerated oxidation of ABTS. The effect of adding MTs to such a solution is used to quantify the MTs by a kinetic assay. Changes in absorbance at 416 nm are linearly correlated to the concentration of MTs in the 4.3 to 49 nM range, and the detection limit is 1.3 nM. The method was successfully applied to the determination of MTs in (spiked) human urine. The strategy may pave the way for related detection platforms.
The gold nanoparticle (AuNP)-Hg-metallothionein (MT) hybrid possesses a stronger peroxidase-like activity than the AuNP-Hg complex. It can catalyze H 2 O 2 -mediated oxidation of ABTS along with the change of the absorbance of the assay system, which is directly proportional to the concentration of MTs in the linear range.
研究领域:
分析化学
·
生物医学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Yaqing Chang,Zhe Zhang,Jinhui Hao,Wenshu Yang,Jilin Tang
The detection of glyphosate (Glyp) in water is of increasing importance because of its potential danger to environmental and health. In this study, a simple and label free colorimetric method for Glyp detection is proposed based on the inhibition of peroxidase-like activity of Cu 2+ . Cu 2+ possesses the peroxidase-like activity that could catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to oxidized TMB (oxTMB) in the presence of H 2 O 2 , resulting in a color change of the solution. However, the peroxidase-like activity of Cu 2+ could be strongly hindered by Glyp due to the formation of Glyp–Cu 2+ complexes. The color change of the solution or the absorbance change of oxTMB is utilized to monitor the concentration of Glyp. The linear range of the proposed method is 2–200 μM and the detection limit is 1 μM. Furthermore, as low as 10 μM Glyp can be clearly distinguished by naked eyes. Also, this proposed method is successfully applied for screening Glyp in real samples and shows great promise for environmental monitoring owing to its simplicity, rapidness and high selectivity.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Materials Today Nano
·
2023
·
IF 10.3
Ting Chen,Baichuan Li,Bochu Wang,Xiaoyong Luo,Zhiyu Fan,He Zhao,Jie Yang,Biye Ren,Yuepeng Cai,Renfeng Dong
Glutathione (GSH) is an important reducing molecule in organisms, and its abnormal levels are closely related to certain diseases. Thus, developing a fast and simple strategy to detect GSH, especially in complex channels such as blood vessels, is of great significance for disease prevention and diagnosis. In this work, a novel peroxidase-like catalytic activity tubular microrocket (FeO x /Pt/PEDOT) with efficient propulsion and steerable motion has been successfully developed for GSH detection. The microrocket fueled by H 2 O 2 exhibits strong propulsion for solution self-stirring and effective reactive oxygen species (ROS) generation to GSH color reaction. Combining these advantages, the catalytically propelled microrocket-treated sample can greatly shorten a half time of detecting reactions and realize efficient GSH detection. In addition, the microrocket shows effective directional detection of GSH in a designed complex channel due to its excellent magnetically navigation, which is of great significance in GSH detection in the microenvironment. This attractive catalytic microrocket has strong propulsion, effective direction control and excellent GSH detection performance, which shows great potential in biodetection and biomedical applications, especially in visual detection of vessels microenvironment.
研究领域:
生物化学
·
医学诊断
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Miaomiao Li,Xiuying Peng,Yujie Han,Lifang Fan,Zhiguang Liu,Yujing Guo
MXenes are attractive for various biosensors owning to their tunable versatile properties. Herein, Ti 3 C 2 nanosheets (Ti 3 C 2 NSs) were found to possess intrinsic peroxidase-like activity, and which could be enhanced by single-stranded DNA (ssDNA) adsorbed on the nanosheets. Hence, a simple label-free sensing strategy was developed for colorimetric detection of biomolecules, based on Ti 3 C 2 NSs as a peroxidase mimic enzyme and ssDNA aptamer as an enzymatic activity enhancement factor along with a recognition substance for the target. In the presence of target biomolecules, the aptamers were desorbed from Ti 3 C 2 NSs due to the specific binding of targets to aptamers, thus bringing about the decrease of catalytic activity. Thrombin (TB) as the model was determined by this constructed biosensor with a linear range of 1.0 × 10 −11 to 1.0 × 10 −8 M, and evaluated subsequently in the real blood samples with satisfactory results. This work highlights Ti 3 C 2 MXenes as a nanomaterial mimic enzyme (called nanozyme) for monitoring biomolecules by target-specific recognition strategy.
研究领域:
生物传感
·
材料科学
·
医学检测
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）