Chemical Engineering Journal Advances
·
2022
·
IF 0
Na Yuan,Hongchao Li,Jieshu Qian
Determination of the concentration of peracetic acid (PAA) in the presence of H 2 O 2 is a great challenging problem due to the similar chemical behaviors of the two species. In this study, we developed a new method for simultaneous measurement of PAA (0.5 – 10.0 μM) and total peroxides (i.e., PAA and H 2 O 2 with a molar ratio of 10:6.5), based on the catalytic oxidation of ABTS by separately using Co(II) and peroxidase (POD) as the catalysts. Owing to the unique catalytic activity of Co(II) towards PAA, the coexistent H 2 O 2 exhibits negligible effect on the determination of PAA. The value of absorbance at 415 nm increases linearly with increasing the concentration of PAA or total peroxides, and the sensitivities for PAA and total peroxides are as high as 5.31 × 10 4 and 9.07 × 10 4 M −1 cm −1 , respectively. In addition, the proposed ABTS method shows excellent feasibility and applicability, which make it suitable to be employed for detection in complicated water matrices.
研究领域:
分析化学
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环境科学
✓ 已验证
DOI
CHEMOSPHERE
·
2017
·
IF 4.208
Huahua Cai,Xin Liu,Jing Zou,Junyang Xiao,Baoling Yuan,Fei Li,Qingfeng Cheng
In this study, a new spectrophotometric method was proposed for the measurement of hydrogen peroxide (H 2 O 2 ) in aqueous solutions. The method was based on the peroxidase (POD)-catalyzed reaction in which 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) was oxidized to form the stable green radical (ABTS + ). The generated ABTS + could be determined spectrophotometrically. The absorbance of the generated ABTS + at 415 nm, 650 nm, 732 nm and 820 nm were linear with H 2 O 2 concentrations in the range of 0–40 μM. The sensitivities of the proposed ABTS method for H 2 O 2 determination at 415 nm, 650 nm, 732 nm and 820 nm were 6.29 × 10 4 M −1 cm −1 , 2.00 × 10 4 M −1 cm −1 , 2.54 × 10 4 M −1 cm −1 and 1.89 × 10 4 M −1 cm −1 , respectively. The oxidation of ABTS to generate ABTS + in POD-catalyzed H 2 O 2 system at pH 6.0 was so fast that the determination time of the ABTS method was as short as 0.5 min. The stoichiometry of the reaction of H 2 O 2 and ABTS in the presence of POD was calculated as nearly 1:2 (1:1.92). The residual absorbance of the generated ABTS + was also found to be stable within 30 min in natural waters. Low H 2 O 2 concentration in rainwater could be both measured by the ABTS method and the DPD method with high accuracy, however, H 2 O 2 concentration in wastewater contained rhodamine B could only be accurately measured with the ABTS method at 732 nm. Moreover, waste solutions after H 2 O 2 analysis with the proposed ABTS method were non-hazardous towards E. coli .
研究领域:
分析化学
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环境科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2019
·Vol. 11
·期 14
·1930-1938
·
IF 2.378
Junyang Xiao,Mengyun Wang,Zijun Pang,Lin Dai,Jinfeng Lu,Jing Zou
Recently, increasing attention has been paid for using commercial peracetic acid (PAA) solution as an efficient disinfectant or a strong oxidant in water treatment. Simultaneous determination of PAA and hydrogen peroxide (H2O2) is vital because PAA solution is commonly available as a mixed solution containing the peroxides PAA and H2O2. In this study, a simple, rapid and reliable spectrophotometric method for the simultaneous determination of PAA and H2O2 was developed. This method was based on the rapid oxidation of excess potassium iodide by PAA and H2O2 in the presence of ammonium molybdate to yield yellow-colored triiodide ions (I3−) that could be quantitatively measured using an UV-Vis spectrophotometer. The absorption of generated I3− at 350 nm increased linearly (R2 > 0.999) with the concentrations of total peroxides and PAA in the range of 0–70 μM. The sensitivities were respectively measured to be as high as 2.596 × 104 M−1 cm−1 for total peroxides and 2.616 × 104 M−1 cm−1 for PAA, while the corresponding limits of detection were calculated to be as low as 0.19 μM and 0.18 μM. The concentration of the H2O2 coexisting in PAA solution was obtained by subtracting the concentration of PAA from the concentration of total peroxides. The concentrations of total peroxides, PAA and H2O2 in practical water samples measured with this proposed method were in good agreement with the values measured with the earlier reported spectrophotometric method using N,N-diethyl-p-phenylenediamine as the indicator. Therefore, this proposed spectrophotometric method could be considered as an alternative method for the simultaneous determination of PAA and H2O2 coexisting in PAA solution.
研究领域:
分析化学
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环境科学
✓ 已验证
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
·
2019
·
IF 6.97
Chao Yang,Zhenyang Xie,Hongping Zhang,Hua Li,Yanhua Cai,Jinhu Wang,Jiayan Du,Haili Yu,Yi He
The traditional nanozymes have low atom/ion utilization efficiency and are easily influenced by the size, morphology, as well as surface composition of nanomaterials. Herein, we report a template-free and valid approach to prepare a ferrous ion (Fe2+) anchored onto the surface of the MoO3 nanosheet (Fe2+/MoO3) through coordination interaction between Fe2+ and hydroxyl groups of MoO3. The resulting Fe2+/MoO3 shows the peroxidase-like activity, and it can be utilized for catalytic decomposition of hydrogen peroxide (H2O2) to generate hydroxyl radicals (•OH) that oxidize various chromogenic substrates, generating distinct color change and strong ultraviolet–visible absorption. The Fe2+/MoO3 peroxidase mimic exhibits a good thermal stability and works well at a high reaction temperature (95 °C) and has higher affinity for substrates than for natural horseradish peroxidase. Theoretical calculation results imply that the strong electrostatic field from the polarization charges between Fe2+ and the MoO3 nanosheet improves the reaction activity, which boosts the generation of •OH from H2O2. Using the Fe2+/MoO3 peroxidase mimic-involved chromogenic system, we construct visual colorimetric assays for H2O2 and triacetone triperoxide (TATP) explosives, which realize the visual detection of H2O2 and TATP at concentrations of 40 and 60 μM, respectively.
研究领域:
材料科学
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分析化学
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纳米技术
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2016
·
IF 5.31
Hefei Wang,Yongsheng Zhao,Tianyi Li,Zhen Chen,Yinan Wang,Chuanyu Qin
Calcium peroxide (CaO 2 ), as one of the solid peroxides, has been increasingly used in contaminated site remediation due to its ability to release oxygen (O 2 ) and hydrogen peroxide (H 2 O 2 ). Biodegradation can be enhanced by the existence of O 2 . Hydrogen peroxide can be used to for in situ chemical oxidation via the modified Fenton . Previous studies show inconsistent results about O 2 and H 2 O 2 release during CaO 2 decomposition. Additionally, the release characteristics of O 2 and H 2 O 2 are not evaluated in detail. A series of batch experiments was conducted to investigate the properties of CaO 2 decomposition and the release kinetics of O 2 and H 2 O 2 . O 2 and H 2 O 2 were released directly from CaO 2 dissolution and H 2 O 2 was not an essential intermediate in the conversion of CaO 2 to O 2 . The two release paths formed a parallel reaction system, with a competitive relationship between the two paths. The release of H 2 O 2 and O 2 followed a pseudo-zero-order kinetics pattern and a pseudo-first-order kinetics pattern, respectively. Increasing temperature and decreasing pH could accelerate both O 2 and H 2 O 2 release rate. However, increasing temperature could decrease the H 2 O 2 yield and increase the O 2 yield, which were similar to the impact of increasing pH.
研究领域:
环境科学
·
化学工程
✓ 已验证
DOI
高影响因子
ELECTROCHIMICA ACTA
·
2022
·
IF 7.336
Lu Yang,Nan Wu,Rongrong Bai,Minjiao Chen,Weiliang Dong,Jie Zhou,Min Jiang
We report a facile strategy for the detection of pyruvate in fermentation progress based on a non-enzymatic decarboxylation reaction of pyruvate with hydrogen peroxide (H 2 O 2 ), coupled with a horseradish peroxidase (HRP)-incorporating nanomaterial as an enzyme element to replace conventional pyruvate oxidase (POX). HRP-Cu 3 (PO 4 ) 2 •3H 2 O nanoflowers (HRP-CuP NFs) and thiol graphene (tG) were immobilized onto an Au disk electrode via layer-by-layer assembly to construct an improved amperometric H 2 O 2 biosensor. The as-prepared HRP-CuP NF/tG/Au electrode exhibited a high electrocatalytic performance toward H 2 O 2 , showing a sensitive response of 67.6 μA mM -1 cm -2 , as well as a linear range from 0.1-8.2 mM with a detection limit (LOD) of 0.06 mM (S/N=3). Furthermore, the resulting biosensor also demonstrated a linear range for pyruvate detection from 1.0 to 8.0 mM with LOD of 0.3 mM (S/N=3), under a working potential of -0.3 V. Finally, the biosensor was successfully used for the determination of pyruvate in real microbial fermentation processes without pretreatment, showing an excellent correlation coefficient compared to the standard HPLC assay with RSD values of 3.03-6.45%. This work provides a new approach for the design and preparation of portable sensors for indirect pyruvate detection with high performance, low cost, and easy operation.
研究领域:
生物化学
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传感器技术
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发酵工程
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产品关联
DOI
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 75
·47435-47447
·
IF 3.108
Donglin He,Huiping Zhang,Ying Yan
CuO over ZSM-5 zeolite membrane was prepared by chemical vapor deposition (CVD) for catalytic wet peroxide oxidation (CWPO) of phenol in a fixed bed reactor. Firstly, Cu-ZSM-5/PSSFs catalysts with Cu loading of 2 wt%, 4 wt% and 6 wt% were prepared and characterized by XRD, XPS, SEM, EDS, N2 adsorption–desorption and TPR (H2), respectively. Compared with CuO deposited directly onto PSSFs, the CuO supported on ZSM-5/PSSFs tended to be more regular with smaller crystal size. Then, several parameters affecting H2O2 consumption and phenol oxidation such as reaction temperature, Cu loading, and different supports were investigated in the CWPO of phenol with a high concentration (1 g L−1 phenol and 5.1 g L−1 H2O2). Experimental results revealed that Cu-ZSM-5/PSSFs (6%) showed better activity for the oxidation of phenol than Cu-ZSM-5/PSSFs catalysts with lower Cu loading and CuO/PSSFs with same theoretical loading. A complete reduction of phenol and a high TOC removal around 60% had been achieved over Cu-ZSM-5/PSSFs (6%) at the temperature of 80 °C, feed flow rate of 2 mL min−1 and catalyst bed height of 2 cm. Finally, the possible oxidation pathway of phenol was studied based on the by-products detected by high-performance liquid chromatography (HPLC).
研究领域:
环境科学
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化学工程
·
材料科学
✓ 已验证
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
·
2021
·
IF 8.198
Xuefang Zheng,Qi Lian,Liya Zhou,Yanjun Jiang,Jing Gao
A simple and efficient strategy was developed to synthesize polyacrylonitrile-copper oxide (PAN-CuO) nanoflowers by growing copper oxide nanoneedles on the surface of polyacrylonitrile (PAN) nanoflowers under mild conditions. Fortunately, the obtained PAN-CuO nanoflowers exhibit excellent peroxidase-mimicking activity to catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to form a blue-colored solution with H2O2. The peroxidase-like mechanism originated from the generation of the •OH radical. The PAN-CuO-based colorimetric assay was used to detect H2O2 with a detection limit (LOD) of 0.12 μM in a linear range from 0.5 to 125 μM. The colorimetric method was simple, selective, and sensitive for ascorbic acid (AA) detection in the range of 1–180 μM with a detection limit (LOD) of 0.56 μM. Moreover, the proposed PAN-CuO-based colorimetric assay could be used in the real sample analysis. The peroxidase-like PAN-CuO nanoflowers could provide a versatile catalytic platform for the application of the colorimetric assay method and biomimetic enzyme catalytic systems, and open a new way for detection of AA in biological systems.
研究领域:
材料科学
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生物化学
·
分析化学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
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2022
·
IF 9.231
Xin Jia-ying,Lu Xue-chun,Sun Li-rui,Zhang Jia-yu,Xia Chun-gu
Calcium peroxide is forbidden to be added to flour as brightener in many countries. A rapid and sensitive spectrophotometric method for the detection of calcium peroxide in flour was proposed. Methanobactin (Mb), a copper-binding small peptide of methanotrophs with excellent peroxidase-like activity, has been successfully applied for H 2 O 2 -mediated co-oxidation between phenol and 4-aminoantipyrine to give a colored product that can be detected at 505 nm. When the concentration of Mb-Cu was 6.5 × 10 −6 mol/L, the detection temperature was 50 ℃, and the detection time was 5 min, the linear range for quantification of calcium peroxide concentration was observed between 0.4 and 10.0 mg/L with R 2 = 0.99397. The limit of detection was 0.027 mg/L (3.34 mg/kg flour) and the average recovery of standard addition was 99.6%–100.5%. Mb was very stable and still maintained catalytic activity even at 50 ℃ in acidic medium, which gave the proposed method has simple process and rapid detection speed.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2020
·
IF 3.448
Chuankai Yang,Miaorong Zhang,Wei Wang,Yao Wang,Jianguo Tang
A colorimetric platform based on enzyme-inorganic hybrid nanoflowers was used for ultraviolet-visible detection of hydrogen peroxide (H 2 O 2 ). The hybrid nanoflowers were prepared via incubation at room temperature using horseradish peroxidase (HRP) and copper phosphate (Cu 3 (PO 4 ) 2 ) as the organic and inorganic components, respectively. HRP/Cu 3 (PO 4 ) 2 hybrid nanoflowers (HRP-HNFs) showed linear H 2 O 2 detection range from 100 nM to 100 μM, moreover, HRP-HNFs exhibited good selectivity for H 2 O 2 detection due to the specificity of HRP. In addition, HRP-HNFs have good reusability, excellent color stability and long-term storage stability, which indicate HRP-HNFs are promising for catalysis and biomedical applications.
研究领域:
生物化学
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分析化学
·
纳米材料科学
✓ 已验证
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2020
·Vol. 45
·期 4
·2030-2037
·
IF 3.288
Shuai Han,Xiaohan Chen,Yifei Fan,Yuexing Zhang,Zhongdong Yang,Xia Kong,Zhenxue Liu,Qingyun Liu,Xianxi Zhang
High catalytic activity is essential for constructing a rapid and facile colorimetric sensor to determine H2O2 and interrelated biomolecules. CuCo2O4 microspheres were successfully prepared using a facile hydrothermal and pyrolysis method. The excellent peroxidase-like performance of CuCo2O4 microspheres was evaluated by employing a chromogenic substrate, colorless 3,3′,5,5′-tetramethylbenzidine (TMB), which can be catalytically oxidized by H2O2 into a blue ox-TMB product within only 1 min. Fluorescence results reveal that the catalytic activity of CuCo2O4 is attributed to the increasing amount of ˙OH from the decomposition of H2O2 and theoretical simulations by Materials Studio proved that the existence of a large number of oxygen vacancies effectively enhanced the catalytic performance. Based on the high catalytic activity of CuCo2O4 microspheres, a rapid colorimetric sensor with high selectivity has been designed for detecting H2O2 and ascorbic acid. Under optimal reaction conditions, the linear response ranges of H2O2 and AA are 1.00–10.00 mM and 1.00–10.00 μM. The detection limits of H2O2 and AA are 132.70 μM and 0.57 μM, respectively.
研究领域:
生物化学
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材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 47
·期 39
·18476-18484
·
IF 3.3
Bin Liu,Haoliang Ruan,Chengyi Li,Jie Yao,Bei Wei,Lei Wang,Shurong Ban,Jun Xie
In this work, InCex nanoparticles with different In–Ce ratios were synthesized by a simple co-precipitation method and InCex nanoparticles could be used as a mimic of peroxidase to catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by H2O2 and produce the typical blue ox TMB. In addition, the prepared InCex nanoparticles showed excellent peroxidase-like catalytic properties compared with pure CeO2 and pure In2O3. InCe0.1 exhibited the best peroxidase-like activity, which was mainly attributed to the indium–cerium synergy, which accelerated the TMB and H2O2 electron transfer rate between them. Based on the above findings, a facile and sensitive H2O2 detection system with a sensitive response range of 10–100 μM and a detection limit of 5 μM for H2O2 was successfully proposed. This work synthesizes nanomaterials with peroxidase-like activity by modifying In2O3 through a simple co-precipitation method and offers new insights into the search for novel peroxidase mimics.
研究领域:
生物化学
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材料科学
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）