MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Xuanxuan Hao,Zhimin Liu,Yaxin Li,Waner Hou,Tongtong Zheng,Leqian Hu
In this study, a near-infrared (NIR) electrochemiluminescence (ECL) emitter CdIn 2 S 4 was synthesized by a convenient hydrothermal strategy. The ECL properties of the obtained CdIn 2 S 4 were examined in the presence of potassium persulfate coreactant at an excitation potential of − 1.25 V. The low trigger voltage of CdIn 2 S 4 may be ascribed to its lower bandgap. This lower excitation potential could effectively reduce the side-effects induced by high voltages, while maintaining the biological activity of antibodides and antigens. The ECL features of CdIn 2 S 4 were also investigated, demonstrating that CdIn 2 S 4 exhibited excellent NIR characteristics, and surface state transitions led to the ECL emission. Importantly, CdIn 2 S 4 was introduced into ECL and electrochemical impedance spectroscopy (EIS) to establish a dual-mode immunosensing interface. Meanwhile, AuNPs@g-C 3 N 4 with excellent properties were used to promote electron transmission and increase the immobilization amount of zearalenone (ZEA) antibodies in the dual-mode sensing system. The developed immunosensing strategy displayed superior sensing performance in ZEA analysis. The detection limits of ZEA were 30 fg mL −1 (ECL model) and 10 fg mL −1 (EIS model), respectively. The desirable recovery results of ZEA in corn samples prove the application potential of the NIR emitter CdIn 2 S 4 in developing a dual-mode immunosensing system for early real-time use.
研究领域:
分析化学
·
生物医学工程
·
食品安全检测
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Huiping Lv,Ruizhong Zhang,Shanshan Cong,Jinna Guo,Mingzheng Shao,Wendong Liu,Libing Zhang,Xiaoquan Lu
Exploiting low cost, water-soluble, and near-infrared (NIR) emissive electrochemiluminophores (ECLphores) is significantly important for biological applications. In this study, bright and NIR electrogenerated chemiluminescence (ECL) emissive copper nanoclusters (Cu NCs) were synthesized through a facile one-pot wet chemical reduction method. ECL properties of obtained Cu NCs were examined in the presence of potassium persulfate, resulting in maximum intensity at 735 nm, at least 135 nm red-shifted with respect to all other Cu NCs. Electrochemistry, photoluminescence (PL), and spooling ECL spectroscopies were used to track NIR ECL emission of Cu NCs ascribed to the monomeric excited states. Due to the abundant binding sites of bovine serum albumin (BSA) to anchor target biomolecules, a sandwich-type ECL immunosensor was thus fabricated using such BSA-templated Cu NCs as tags and alpha fetoprotein antigen (AFP) as a model protein for the first time. Without assisting any signal amplification strategies, the proposed NIR ECL biosensor exhibited a wide linear range (1–400 ng mL–1) and low detection limit (0.02 ng mL–1) as well as superior selectivity and reproducibility and was successfully applied in real human serum sample determination. This work sets the stage for the development of novel non-noble metal nanoclusters for large-scale and emerging nanotechnology applications.
研究领域:
分析化学
·
生物医学工程
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Jie Wu,Ling Wu,Mengmeng Guo,Wen Hao,Lin Cai,Guozhen Fang,Shuo Wang
Enrofloxacin (ENR) residues in animal derived foods are harmful to human health. A molecularly imprinted electrochemiluminescence sensor was constructed for ENR detection utilizing a self-enhanced quasi-three-dimensional luminescent-composite of boron nitride quantum dots/nitrogen-doped mesoporous carbon crosslinked with copper nanowires (BNQDs/NMC-CuNWs). One-dimensional CuNWs with near-infrared electrochemiluminescence (NIR-ECL) properties were inserted into the electroactive layer of two-dimensional NMC to form quasi-three-dimensional NMC-CuNWs, which inhibited the aggregation of CuNWs and increased the electron and active ion transport rates. BNQDs were also embedded into the NMC-CuNWs network as co-reactant accelerators and antioxidants. A molecularly imprinted polymer containing bifunctional monomers enabled the specific detection of ENR. The detection range of ENR was 1.00 × 10 −9 –5.00 × 10 −5 mol L −1 , with a low limit of detection (3.33 × 10 −10 mol L −1 ). The recovery rate of ENR was 83.92 %–94.56 %, which is consistent with the high-performance liquid chromatography results. This work offers new ideas for detecting hazardous substances in the NIR-ECL field.
研究领域:
食品安全检测
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2025
·Vol. 14
·期 3
·538
·
IF 5.1
Jie Wu,Yuanjie Qin,Xiaoxin Mei,Lin Cai,Wen Hao,Guozhen Fang
Ciprofloxacin (CIP), a widely used broad-spectrum antibiotic, poses a serious threat to human health and environmental safety due to its residues. The complementary monomers molecularly imprinted electrochemiluminescence sensor (MIECLS) based on a polyvinylpyrrolidone-functionalized copper nanowires (CuNWs@PVP) luminescent probe was constructed for the ultra-sensitive detection of CIP. CuNWs with low cost and high conductivity exhibited near-infrared electrochemiluminescence (NIR ECL) properties, yet their self-aggregation and oxidation led to a weakened emission phenomenon. PVP with solvent affinity and large skeleton was in situ attached to CuNWs surface to avoid CuNWs sedimentation and aggregation, and self-enhanced ECL signals were achieved. The bifunctional monomers molecularly imprinted polymer (MIP) possessed complementary active centers that increased their affinity with CIP, enhancing the accurate and sensitive detection of the target substances. The linear range of CIP using MIECLS was 5.00 × 10 −9 –5.00 × 10 −5 mol L −1 with a low limit of detection (LOD) of 2.59 × 10 −9 mol L −1 , while the recovery rates of CIP in the spiking recovery experiment were 84.39% to 92.48%. The combination of bifunctional monomer MIP and NIR copper-based nano-luminescent probe provides a new method for the detection of CIP in food.
研究领域:
分析化学
·
食品安全
·
生物传感技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Li Fu,Bin Zhang,Xiaoyan Long,Kena Fu,Xuwen Gao,Guizheng Zou
Screening a novel electrochemiluminescence (ECL) system is crucial to ECL evolution. Herein, an efficient ECL system with less interference and environmental concern under physiological condition is developed via a unique internal Cu(I)/Cu(II) couple cycling amplified strategy by employing the glutathione- and citrate-capped copper indium sulfide (CIS)/ZnS nanocrystals (NCs) as electrochemiluminophore and N2H4·H2O as co-reactant. CIS/ZnS NCs can be electrochemically injected with valence band (VB) hole at 0.46 and 0.87 V (vs Ag/AgCl), and then achieve the same hole-injected states by relocalizing VB holes with the Cu(I) species inside of CIS/ZnS NCs to form internal Cu(II) defects, while each N2H4·H2O molecule can be successively oxidized to two more reducing species N2H3• and N2H2 around 0.10 V, and inject conduction band (CB) electron onto CIS/ZnS NCs for triple times. The internal Cu(I)/Cu(II) couple cycling involved radiative-charge recombination between these VB hole and CB electron eventually enables two efficient near-infrared ECL processes (around 731 nm) at 0.55 and 0.87 V, in which each single nanocrystal may participate in multiple ECL reaction cycles to produce multiple photons for amplified ECL, similar to the tris(bipyridyl)ruthenium(III) based ECL system. The low-triggering-potential ECL process at 0.55 V can be utilized to selectively determine Cu(II) with a wide linear range from 10 and 1500 nM and a limit of detection of 5 nM (S/N = 3). This work presents a NCs engineering and co-reactant selecting combined strategy for further ECL evolution.
研究领域:
分析化学
·
纳米技术
·
电化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2020
·
IF 6.785
Lei Yu,Qiao Zhang,Qi Kang,Bin Zhang,Dazhong Shen,Guizheng Zou
The design and development of novel electrochemiluminescence (ECL) systems with near-infrared (NIR) emission beyond 800 nm are promising for ECL evolution, especially for improving the throughput of the spectrum-resolved multiplexing ECL assay and biological imaging. Herein a biocompatible and environmentally friendly luminophore, that is, the methionine-stabilized Au nanoclusters (Met-Au NCs), are proposed to achieve efficient aqueous ECL of ∼835 nm with triethanolamine as coreactant. The Met-Au NCs not only demonstrate a 75 times enhanced ECL compared with the traditional Au NCs with bovine serum albumin as a capping agent but also can be employed as ECL tags to label proteins with a methionine linker and enable a highly sensitive NIR ECL bioassay. A sandwich-type NIR ECL immunosensor is constructed with the Met-Au NCs as tags and α-fetoprotein (AFP) as a model analyte and exhibits a wide linearity range from 3 fg·mL–1 to 0.1 ng·mL–1 with a limit of detection of 1 fg mL–1 (S/N = 3) as well as desired selectivity.
研究领域:
分析化学
·
生物化学
·
纳米技术
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Li Fu,Pengjie Dong,Zerui Liu,Qi Li,Yingshu Guo
Screening a novel electrochemiluminescence (ECL) system and lowering the ECL triggering potential are essential to ECL evolution. Herein, the near-infrared (NIR) ECL system with low-triggering potential ECL was constructed with weakly reductive tert-butylamine borane as coreactant and mercaptosuccinic acid/citrate (MSA/Cit)-capped Au (MSA/Cit@AuNCs) as luminophores. Toxic-element-free and dual-ligand MSA/Cit@AuNCs were prepared via ligand exchange and utilized as a model for developing unary metal NCs-based luminophores with more enhanced ECL performance than monoligand Au nanocrystals (AuNCs), which exhibited a two hole-injected process at around 0.48 and 0.80 V, respectively. Beneficial to the intrinsic low hole-injected potential of AuNCs, MSA/Cit@AuNCs exhibited similar low-triggering ECL potential at around 0.57 V with the participation of series coreactants or not, originating from the recombination of an internal prestored electron within the conduction band (CB) and electroinjected holes at around 0.25 V. Furthermore, the enhanced low-triggering potential around 0.57 V and NIR ECL around 835 nm of MSA/Cit@AuNCs was eventually obtained with the reductive tert-butylamine borane or N2H4·H2O containing a −C–N single-bond structure merely as coreactant. The low-triggering potential ECL of MSA/Cit@AuNCs/tert-butylamine borane system at 0.57 V can be harnessed to selectively determine a carcinoembryonic antigen (CEA) with one linear range spanning from 2 to 20000 fg/mL and a limit of detection of 0.33 fg/mL (S/N = 3). This study will contribute to a more comprehensive understanding of the ECL mechanism in terms of both regulating NCs and selecting coreactants.
研究领域:
分析化学
·
纳米技术
·
生物化学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）